Figure 26: Bleb Formation - BTX AgilePulse ID User Manual

AgilePulse ™
Needle Array Selection Guide
Item # Spacing
Between Rows
47-0040 4 mm
47-0050 4 mm
47-0060 6 mm
47-0043 4 mm
47-0070 6 mm
45-0121 (requires 5 mm
47-0090 or 47-0091
adapter and 45-0206
handle)
The electric field coverage is presented in Figure 25.
Figure 25: IDA-4-6-2 and IDA-6-6-2 electric field coverage
The recommended bleb placement for the IDA-4-6-2 and IDA-6-6-2 electrodes is shown in Figure 26.
Figure 26: Bleb Formation ID-4-6-2 and ID-6-6-2
The total bleb volume for each is approximately:
Model No. Area
47-0050 8.7 mm 2 x2
47-0060 15.6 mm 2
There is a significant tradeoff between wider row spacing and larger delivery volume and pulse amplitude. As pulse amplitude increases so
does pain of the applied pulse voltage.
22
ID Tutorial
Needles/ Needle
Row Diameter
4 0.3 mm
6 0.3 mm
6 0.3 mm
4 0.3 mm
6 0.7 mm
1 0.3 mm
4 mm
4 mm
Depth Volume (approx) Pulse Voltage V/d= 1000 V/cm
2 mm 50–90 µl
x2 2 mm 100 µl
AgilePulse ID In Vivo Gene Delivery System
Publication 015-101444 Rev 2.0 • www.btxonline.com
Needle Suggested Use
Length
2 mm Small animal, Intradermal
2 mm Small animal, Intradermal, large volume and surface area
2 mm Large animal, Intradermal
3 mm Small animal, Tibialis muscle
10 mm Large Animal, Tibialis
20 mm Small or large animal, Intradermal or Tibialis muscle
6 mm
6 mm
400
600
Pulse Voltage V/d= 1500 V/cm
600
900