Log Retrieval; Pulse Protocol; Figure 20: Log Retrieval Screen; Figure 21: Pulse Protocol Modification - BTX AgilePulse ID User Manual

System Operation

Log Retrieval

All previous experimental delivery logs can be retrieved and saved
onto a USB Key or deleted from internal memory. The interface
consists of a series of chronological sub-folders from which the user
is allowed to double-tap on the touch screen to descend through
the hierarchy. The standard naming sequence is Year, Month, Day,
followed by special folders with the time of day. These time folder
icons show a hand underneath, meaning they contain all files
generated from a single vaccine/transfectante delivery. The " "
folders allow the user to return to the previous level of hierarchy.
Special time folders display basic information on the right hand
side of the screen when selected. Identification numbers, time of
day, and status completion indicators help the user find the correct
vaccine/transfectant delivery run to download.
When selected by a single tap on the touch screen icon, any
level of hierarchy can be saved to a USB Key inserted in the front
panel by pressing the SAVE button. The hierarchy structure will
be maintained and copied to the USB Key. The DELETE button
is also available to remove the hierarchy from internal memory.
This delete is permanent and the lost files cannot be recovered.
A maximum of 20,000 delivery logs can be stored on the internal
system memory.
DEPENDING ON THE NUMBER OF FILES TO BE DELETED, THIS
PROCESS CAN BE SLOW.

Pulse Protocol

The pulse protocol to be delivered at run time is set by pressing
the PROTOCOL button and adjusting parameters via the touch
screen interface. Parameters for three pulse groups are modifiable
by selecting a box and scrolling through the available values.
Subsequent groups are activated by selecting more than one pulse
in the number field and conversely deactivated by selecting zero
pulses in the number field.
The adjusted protocol is saved to internal memory when the HOME
button is pressed. Minimum and maximum parameter values are as
follows:
Amplitude (volts)
Minimum 50
Maximum 1000
* For Pulse Amplitudes greater than 300 V, the maximum allowable Pulse Width is 1 ms
** Pulse duty cycle is limited to 50%
IMPORTANT! The changes made on this screen will only be permanently saved when the HOME button is pressed
to go back to the Welcome Screen. If modifications are made and system power is turned off while still viewing the
screen, all changes will be lost.
Caution
16
Protocol Parameter Boundaries
Duration (ms) Interval (ms)
0.050 0.200 (5 kHz)
10* 1000 (1 Hz)
AgilePulse ID In Vivo Gene Delivery System
Publication 015-101444 Rev 2.0 • www.btxonline.com

Figure 20: Log Retrieval Screen

Figure 21: Pulse Protocol Modification

Pulse # Pulse Groups
1 1
10 3