Experiment Delivery Screen Functions And Operation; Figure 12: Delivery Screen - Initialization; Figure 13: Delivery Screen - Load Estimation; Figure 14: Delivery Screen - High Voltage Power Supply Charge - BTX AgilePulse ID User Manual

System Operation
Experiment Delivery Screen Functions
and Operation
Figure 12: Delivery Screen — Initialization
The purpose of the delivery screen is to initiate pulses to deliver
transfectant to tissue. The procedure is as follows.
1. The researcher injects the transfectant forming a bleb under
the skin of the animal, and inserts the electrode
according to the approved protocol.
2. The researcher determines if the electrode is inserted
properly by touching the LOAD button or by depressing
the foot pedal. A number will appear in the Est. Load
box. LOAD button will change to a green color. Estimated
load in ohms is displayed. This number is the effective
resistance of the animal's skin and will continue to be
updated every second.
Figure 13: Delivery Screen — Load Estimation
3. If the LOAD Reading is within range (Less than 3500
ohms for skin) then the next step is to turn on the high
voltage power supply by touching the READY button or
depressing the foot pedal. The READY button will flash
green until fully charged and then will stay solid green.
The high voltage reading will appear in the System
message box. If the researcher wants to abort the process,
the electrode should be removed and the DONE button
pressed. In this case, an early termination message will
appear in the data log and the system reset.
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4. If the researcher determines that the system voltage is
5. When the system reaches a shutdown state either by
AgilePulse ID In Vivo Gene Delivery System
Publication 015-101444 Rev 2.0 • www.btxonline.com
Figure 14: Delivery Screen — High Voltage Power Supply Charge
correct, pulsing is initialized by touching START or
depressing the foot pedal. The START button turns a
solid blue while pulsing is active and solid green when
pulsing is completed.
Figure 15: Delivery Screen — Pulse Waveform Active
successful waveform delivery or by error, all buttons will
turn solid green and pulse delivery data will be
displayed in the System message text box. The load
message box will change from the estimated load value
to the actual high voltage pulse monitored value.