Application; Experimental Delivery Methods - BTX AgilePulse ID User Manual

AgilePulse ™
Applications
The AgilePulse™ system is used to deliver molecules of interest to skin or muscle tissuesn for research purposes only. The AgilePulse system
has been shown to increase DNA delivery to cells resulting in 100 to 1000 times higher protein expression compared to hypodermic needle
injection alone (Roos et al., 2006).
The AgilePulse system is typically used to deliver cancer vaccines in doses of 50 µg or more. The AgilePulse system includes a AgilePulse
voltage waveform generator and an Intradermal Array (IDA) electrode used to convert the voltage to electric fields which deliver the
vaccine directly into cells in the skin.
Transfectant Delivery Methods
There are several methods used to deliver transfectants of interest.
System Name Company
AgilePulse BTX
PMED ™ PowderMed
Biojector ® 2000 Bioject Medical
Technologies
ECM 830, BTX and others
Gemini X2,
and others
Many Many
17
ID Tutorial
Mechanism
Inject transfectant in dermis
or muscle with hypodermic
needle, insert needle array,
apply electric field to deliver
into cells
Microscopic particles coated
with DNA, delivered into cells
using compressed helium
Inject using CO pressure with
2
spacer (subcutaneous) or
without spacer (to muscle)
Inject into muscle insert
electrode and apply electric
field
Deliver viral vector with
hypodermic needle into
muscle
AgilePulse ID In Vivo Gene Delivery System
Publication 015-101444 Rev 2.0 • www.btxonline.com
Target Factors
(All pain ratings are estimates.)
Dermis/Tibialis muscle • Large dose
• Reduced pain compared to
• Pain 3 out of 10
• High cell transfection
Epidermis • Small doses
• Possible tattooing
• Pain 3 out of 10
• Low/Medium cell transfection
Skin or Muscle • Large doses
• Pain 3 out of 10
• Medium cell transfection
Muscle • Large dose
• Significant pain
• Pain 5 to 6 out of 10
• High cell transfection
Muscle • Immune system reacts to viral
• Pain 2 out of 10
other electroporation systems
vector, inefficient when boosting