Agilepulse In Vivo/Ex Plant Electroporation Protocol; Figure 25: Electrical Field Coverage - BTX AgilePulse ID User Manual

AgilePulse ™

AgilePulse In Vivo/Ex Plant Electroporation Protocol

Tissue or Target: Animal Skin, Tibialis Muscle
Transfectant: Plasmid DNA, mRNA, or Protein, or other desired molecule of interest
Electrode Accessory: Please see page 22 for needle array selection guide by animal size and muscle type
Electroporation settings for 4 mm gap electrodes (small animal)
Group
Voltage Groups 1
2
3
Desired Field Strength: 1125 volts/cm
Electroporation settings for 5 mm gap electrodes (small animal, large animal)
Group
Voltage Groups 1
2
3
Desired Field Strength: 1125 volts/cm
Electroporation settings for 6 mm gap electrodes (large animal)
Group
Voltage Groups 1
2
3
Desired Field Strength: 1125 volts/cm
Note: These are recommended electroporation settings. Reducing the pulse interval time below 50 ms in group 2, can lead to error 19.
The interval time can be increased to 300 ms or more, if the error recurs. Please contact BTX technical support if an error persists.
AgilePulse Set Up
• Plug-in the needle array handle cable to the AgilePulse generator.
• Turn on AgilePulse™ system.
• Program the appropriate protocol for your electrode as outlined in tables above.
• Input animal ID and Transfectant ID information on AgilePulse instrument.
• Insert needle array electrode tip into needle array handle. Inspect the needle array electrode to ensure that the electrode needles are
not bent and all are the same length.
• Optional: For hands-free operation, connect foot pedal to one of the USB ports on the AgilePulse generator.
• Optional: Connect USB flash drive to one of the USB ports on the AgilePulse generator for copy of log data on flash drive. Log data is
also stored on Internal Memory on AgilePulse generator automatically.
23
ID Tutorial
Voltage
450 V
450 V
110 V
Voltage
560 V
560 V
140 V
Voltage
680 V
680 V
170 V
AgilePulse ID In Vivo Gene Delivery System
Publication 015-101444 Rev 2.0 • www.btxonline.com
Pulse Length Pulse Interval
0.05 ms 0.2 ms
0.05 ms 50 ms
10 ms 20 ms
Pulse Length Pulse Interval
0.05 ms 0.2 ms
0.05 ms 50 ms
10 ms 20 ms
Pulse Length Pulse Interval
0.05 ms 0.2 ms
0.05 ms 50 ms
10 ms 20 ms
No. Pulses
1
1
8
No. Pulses
1
1
8
No. Pulses
1
1
8