Hanna Instruments HI83303 Instruction Manual
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Hanna Instruments HI83303 Instruction Manual

Aquaculture photometer
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HI83303
Aquaculture Photometer

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Summary of Contents for Hanna Instruments HI83303

  • Page 1 HI83303 Aquaculture Photometer...
  • Page 2 If you need additional technical information, do not hesitate to e‑mail us at tech@hannainst.com or view our worldwide contact list at www.hannainst.com. All rights are reserved. Reproduction in whole or in part is prohibited without the written consent of the copyright owner, Hanna Instruments Inc., Woonsocket, Rhode Island, 02895, USA.

  • Page 3: Table Of Contents

    1. PRELIMINARY EXAMINATION .................... 2. SAFETY MEASURES......................3. SPECIFICATIONS ......................4. DESCRIPTION........................4.1. GENERAL DESCRIPTION..................... 4.2. PRECISION AND ACCURACY..................4.3. FUNCTIONAL DESCRIPTION..................4.4. PRINCIPLE OF OPERATION..................4.5. OPTICAL SYSTEM...................... 5. GENERAL OPERATIONS..................... 5.1. POWER CONNECTION AND BATTERY MANAGEMENT............5.2.
  • Page 4 8. METHOD PROCEDURES....................8.1. ALKALINITY....................... 8.2. ALKALINITY, MARINE....................8.3. AMMONIA LOW RANGE....................8.4. AMMONIA MEDIUM RANGE..................8.5. AMMONIA HIGH RANGE..................... 8.6. CALCIUM........................8.7. CALCIUM, MARINE....................8.8. CHLORINE, FREE....................... 8.9. CHLORINE, TOTAL..................... 8.10. COPPER LOW RANGE....................8.11. COPPER HIGH RANGE....................8.12.

  • Page 5: Preliminary Examination

    Remove the instrument and accessories from the packaging and examine it carefully to make sure that no damage has occurred during shipping. Notify your nearest Hanna Customer Service Center if damage is observed. Each HI83303 is supplied with: • Sample Cuvette and Cap (4 pcs.) • Cloth for Wiping Cuvettes •...

  • Page 6: Specifications

    3. SPECIFICATIONS 5 x optical channels; Measurement Channels 1 x digital electrode channel (pH measurement) Range 0.000 to 4.000 Abs Resolution 0.001 Abs Accuracy ±0.003 Abs (at 1.000 Abs) Light Source light emitting diode Bandpass Filter Bandwidth 8 nm Absorbance Bandpass Filter Wavelength Accuracy ±1.0 nm Light Detector silicon photocell...

  • Page 7: Description

    4. DESCRIPTION 4.1.GENERAL DESCRIPTION HI83303 multiparameter photometer is compact and versatile meter with two measurement modes: Absorbance and pH/ mV. Absorbance mode include CAL Check feature and 25 different methods that cover a wide variety of applications, making it ideal for both benchtop and portable operation.

  • Page 8: Functional Description

    4.3. FUNCTIONAL DESCRIPTION Splash‑proof keypad Liquid Crystal Display (LCD) Indexing mark Protective port covers Light‑blocking cover panel Cuvette holder ON/OFF power button 3.5 mm TRRS (jack) input for digital electrodes Standard USB host connector for data transfer to a USB flash drive 10) Micro‑USB device connector for power or PC interface...

  • Page 9: Principle Of Operation

    Keypad Description The keypad contains 12 direct keys and 3 functional keys with the following functions: Press the functional keys to perform the function displayed above them on the LCD. Press to access the list of photometer methods. Press to move up in a menu or a help screen, to increment a set value, or to access second level functions.

  • Page 10: Optical System

    4.5. OPTICAL SYSTEM Instrument Block Diagram The internal reference system (reference detector) of the HI83303 photometer compensates for any drifts due to power fluctuations or ambient temperature changes, providing a stable source of light for your blank (zero) measurement and sample measurement.

  • Page 11: General Operations

    5. GENERAL OPERATIONS 5.1. POWER CONNECTION AND BATTERY MANAGEMENT The meter can be powered from an AC/DC adapter (included) or from the built‑in rechargeable battery. The meter will perform an auto‑diagnostic test when it is first powered on. During this test, the HANNA logo will appear on the LCD.
  • Page 12 Temperature Unit (pH Only) Option: °C or °F Press the functional key to select the desired temperature unit. Backlight Values: 0 to 8 Press the Modify key to access the backlight intensity. Use the functional keys or the   keys to increase or decrease the value.
  • Page 13 Date Format Press the Modify key to change the Date Format. Use the   keys to select the desired format. Press the Select key to confirm or ESC to return to the Setup menu without saving the new format. Decimal Separator Option: Comma ( , ) or Period ( .

  • Page 14: Using Hanna Digital Electrodes

    Press the Select key to view model number, serial number and firmware version for the connected probe. Press ESC to return to the Setup menu. 5.3. USING HANNA DIGITAL ELECTRODES HI83303 can be used to perform direct pH measurements by connecting a HANNA digital ®...

  • Page 15: Logging Data

    5.5. LOGGING DATA The instrument features a data log function to help you keep track of all your analysis. The data log can hold 1000 individual measurements. Storing, viewing and deleting the data is possible using the LOG and RECALL keys. Storing data: You can store only a valid measurement.

  • Page 16: Data Management

    The following functions are available during Text Entry: • Accept: Press to accept the current displayed text. • Arrow: Press to delete the last character. • Clear: Press to delete all characters. Press to discard all changes and return to the previous screen. 5.7.

  • Page 17: Contextual Help

    Log data is exported as a single file containing all logged photometer and probe data. The file name is: “HI83303.csv”. The CSV file (Comma‑Separated Values) may be opened with a text editor or spreadsheet application. 5.8. CONTEXTUAL HELP HI83303 offers an interactive contextual help mode that assists the user at any time.

  • Page 18: Collecting And Measuring Samples And Reagents

    6.2. COLLECTING AND MEASURING SAMPLES AND REAGENTS 6.2.1. PROPER USE OF SYRINGE (a) Push the plunger completely into the syringe and insert the tip into the solution. (b) Pull the plunger up until the lower edge of the seal is exactly on the mark for the desired volume.

  • Page 19: Cuvette Preparation

    6.3. CUVETTE PREPARATION Proper mixing is very important for reproducibility of the measurements. The proper mixing technique for each method is listed in the method procedure. (a) Invert the cuvette a couple of times or for a specified time: hold the cuvette in the vertical position.

  • Page 20: Timers And Measurement Functions

    Do not let the reacted sample stand too long after reagent is added. For best accuracy, respect the timings described in each specific method. It is possible to take multiple readings in a row, but it is recommended to take a new zero reading for each sample and to use the same cuvette for zeroing and measurement when possible.

  • Page 21: Chemical Formula / Unit Conversions

    ® Standards. For accurate validation results, please perform tests at room temperature (18 to 25 °C; 64.5 to 77.0 °F). Validation of the HI83303 involves absorbance measurements of certified HANNA CAL Check ® Standards (see “Accessories”). The “CAL Check” screen guides the user through the measurement of each CAL Check Standard and applies the factory calibration corrections to each measurement.

  • Page 22: Absorbance Measurements

    4. Press ESC to return in Setup menu. 6.7. ABSORBANCE MEASUREMENTS Raw absorbance measurements may be performed on the HI83303 for personal or diagnostic purposes. For example, you may monitor the stability of a reagent blank by occasionally measuring its absorbance versus deionized water.

  • Page 23: Probe Mode

    7. PROBE MODE 7.1. pH CALIBRATION Press MODE to enter in pH/ mV measurement mode. Press Calibrate to access electrode calibration functions. Calibration Mode While in pH Calibration Mode, the display will show the current pH reading, the current temperature reading, the current selected buffer, and the buffer number (“Buffer: 1” for the 1st buffer, “Buffer: 2”...
  • Page 24 Preparation Pour small quantities of the buffer solutions into clean beakers. If possible, use plastic beakers to minimize any EMC interferences. For accurate calibration and to minimize cross‑contamination, use two beakers for each buffer solution: one for rinsing the electrode and one for calibration. If you are measuring in the acidic range, use pH 7.01 or 6.86 as the first buffer and pH 4.01 as the second buffer.

  • Page 25: Ph Calibration Messages

    Wrong Temperature: The buffer temperature is too extreme for the selected buffer value. 7.3. pH MEASUREMENT HI83303 can be used to perform direct pH measurements by connecting a HANNA digital ® pH electrode with a 3.5 mm TRRS connector. To begin taking probe measurements, connect the electrode to the 3.5 mm port marked with EXT PROBE located at the rear of the meter.
  • Page 26 Press to switch to Photometer mode. Press to access the meter’s Setup menu. Press to log the current measurement. Press to review the meter’s log history. Press to view contextual help information. For high accuracy it is recommended to calibrate your electrode often. pH electrodes should be recalibrated at least once per week, but daily calibration is recommended.

  • Page 27: Ph Measurement Messages / Warnings

    7.4. pH MEASUREMENT MESSAGES / WARNINGS No Probe: No probe is connected or the probe is broken. Connecting: The meter has detected a probe and is reading the probe configuration and calibration information. Incompatible Probe: The connected probe is not compatible with this device. Incompatible Calibration: The probe’s current calibration is not compatible with this meter.

  • Page 28: Ph Glp

    7.5. pH GLP Good Laboratory Practice (GLP) refers to a quality control function used to ensure uniformity and consistency of sensor calibrations and measurements. To view the GLP information, press the GLP key from the Probe Measurement screen. The pH GLP screen displays the following information about the last pH calibration: •...

  • Page 29: Ph Electrode Conditioning And Maintenance

    7.6. pH ELECTRODE CONDITIONING AND MAINTENANCE Remove the protective cap of the pH electrode. DO NOT BE ALARMED IF SALT DEPOSITS ARE PRESENT. This is normal with electrodes. They will disappear when rinsed with water. During transport, tiny bubbles of air may form inside the glass bulb affecting proper functioning of the electrode.
  • Page 30 Storage Procedure To minimize clogging and ensure a quick response time, the glass bulb and the junction should be kept moist and not allowed to dry out. Replace the solution in the protective cap with a few drops of HI70300 HI80300 Storage Solution or, in its absence, Filling Solution...

  • Page 31: Method Procedures

    8. METHOD PROCEDURES 8.1. ALKALINITY SPECIFICATIONS Range 0 to 500 mg/L (as CaCO Resolution 1 mg/L Accuracy ±5 mg/L ±5% of reading at 25 °C Light Source LED with narrow band interference filter @ 610 nm Method Colorimetric Method. REQUIRED REAGENTS Code Description Quantity...
  • Page 32 • Remove the cuvette. Note: Any chlorine present in the sample will interfere with the reading. To remove the chlorine interference add one drop of HI93755-53 Chlorine Removal Reagent to the unreacted sample. • Add 1mL of HI775S Alkalinity Reagent to the sample using a 1 mL syringe.

  • Page 33: Alkalinity, Marine

    8.2. ALKALINITY, MARINE SPECIFICATIONS Range 0 to 300 mg/L (as CaCO Resolution 1 mg/L Accuracy ±5 mg/L ±5% of reading at 25 °C Light Source LED with narrow band interference filter @ 610 nm Method Colorimetric Method. REQUIRED REAGENTS Code Description Quantity HI755S...
  • Page 34 • Remove the cuvette. • Add 1 mL of HI755S Alkalinity Reagent to the sample using a 1mL syringe. • Replace the cap and gently invert 5 times. • Reinsert the cuvette into the instrument and close the lid. • Press Read to start the reading. The instrument displays the results in mg/L as calcium carbonate (CaCO •...

  • Page 35: Ammonia Low Range

    8.3. AMMONIA LOW RANGE SPECIFICATIONS Range 0.00 to 3.00 mg/L (as NH ‑N) Resolution 0.01 mg/L Accuracy ±0.04 mg/L ±4% of reading at 25 °C Light Source LED with narrow band interference filter @ 420 nm Method Adaptation of the ASTM Manual of Water and Environmental Technology, D1426 Nessler method.
  • Page 36 • Add 4 drops of HI93700A‑0 Ammonia Low Range Reagent A. Replace the cap and mix the solution. • Add 4 drops of HI93700B‑0 Ammonia Low Range Reagent B. Replace the cap and mix the solution. • Reinsert the cuvette into the instrument and close the lid. •...
  • Page 37 • Press  or  to access the second level functions. • Press the Chem Frm key to convert the result to mg/L of ammonia (NH ) and ammonium • Press  or  to return to the measurement screen. INTERFERENCE Interference may be caused by: Acetone...

  • Page 38: Ammonia Medium Range

    8.4. AMMONIA MEDIUM RANGE SPECIFICATIONS Range 0.00 to 10.00 mg/L (as NH ‑N) Resolution 0.01 mg/L Accuracy ±0.05 mg/L ±5% of reading at 25 °C Light Source LED with narrow band interference filter @ 420 nm Method Adaptation of the ASTM Manual of Water and Environmental Technology, D1426, Nessler method.
  • Page 39 • Remove the cuvette. • Add 4 drops of HI93715A‑0 Ammonia Medium Range Reagent A. Replace the cap and mix the solution. • Add 4 drops of HI93715B‑0 Ammonia Medium Range Reagent B. Replace the cap and mix the solution. •...
  • Page 40 • Press the Chem Frm key to convert the result in mg/L of ammonia (NH ) and ammonium • Press  or  to return to the measurement screen. INTERFERENCES Interference may be caused by: Acetone Alcohols Aldehydes Glycine Hardness above 1 g/L Iron Organic chloramines Sulfide...

  • Page 41: Ammonia High Range

    8.5. AMMONIA HIGH RANGE SPECIFICATIONS Range 0.0 to 100.0 mg/L (as NH ‑N) Resolution 0.1 mg/L Accuracy ±0.5 mg/L ±5% of reading at 25 °C Light Source LED with narrow band interference filter @ 420 nm Method Adaptation of the ASTM Manual of Water and Environmental Technology, D1426, Nessler method.
  • Page 42 • Press the Zero key. The display will show “‑0.0‑” when the meter is zeroed and ready for measurement. • Remove the cuvette. • Add 4 drops of HI93733A‑0 Ammonia High Range Reagent A. Replace the cap and swirl the solution. •...
  • Page 43 •Press the Chem Frm key to convert the result to mg/L of ammonia (NH ) and ammonium •Press  or  to return to the measurement screen. INTERFERENCES Interference may be caused by: Acetone Alcohols Aldehydes Glycine Hardness above 1 g/L Iron Organic chloramines Sulfide...

  • Page 44: Calcium

    8.6. CALCIUM SPECIFICATIONS Range 0 to 400 mg/L (as Ca Resolution 1 mg/L Accuracy ±10 mg/L ±5% of reading at 25 °C Light Source LED with narrow band interference filter @ 466 nm Method Adaptation of the Oxalate method. REQUIRED REAGENTS Code Description Quantity...
  • Page 45 • Replace the cap and invert several times to mix. • Place the cuvette into the holder and close the lid. • Press the Zero key. The display will show “‑0.0‑” when the meter is zeroed and ready for measurement. •...
  • Page 46 • After waiting 5 minutes, invert again the cuvette 10 times to mix (about 15 seconds). • Reinsert the cuvette into the instrument and close the lid. • Press Read to start the reading. The instrument displays the results in mg/L of calcium (Ca INTERFERENCES Interferences may be caused by: Acidity (as CaCO...

  • Page 47: Calcium, Marine

    8.7. CALCIUM, MARINE SPECIFICATIONS Range 200 to 600 mg/L (as Ca Resolution 1 mg/L Accuracy ±6% of reading at 25 °C Light Source LED with narrow band interference filter @ 610 nm Method Adaptation of the Zincon method. REQUIRED REAGENTS Code Description Quantity...
  • Page 48 • Press the Zero key. The display will show “‑0.0‑” when the meter is zeroed and ready for measurement. • Remove the cuvette. • Use the minipipette to add 0.1 mL of sample to the cuvette. • Add one packet of HI7582 Calcium Reagent B.

  • Page 49: Chlorine, Free

    8.8. CHLORINE, FREE SPECIFICATIONS Range 0.00 to 5.00 mg/L (as Cl Resolution 0.01 mg/L Accuracy ±0.03 mg/L ±3% of reading at 25 °C Light Source LED with narrow band interference filter @ 525 nm Method Adaptation of the EPA DPD method 330.5. REQUIRED REAGENTS POWDER: Code...
  • Page 50 • Press the Zero key. The display will show “‑0.0‑” when the meter is zeroed and ready for measurement. • Remove the cuvette. POWDER REAGENT PROCEDURE • Add the content of one packet of HI93701‑0 Free Chlorine Reagent. Replace the cap and shake gently for 20 seconds. •...
  • Page 51 • Swirl gently to mix. • Add 10 mL of unreacted sample (up to the mark). 10 mL Replace the cap and shake gently. • Insert the cuvette into the instrument and close the lid. • Press Read to start the reading. The instrument displays the results in mg/L of chlorine (Cl Note: Free and Total Chlorine have to be measured separately with fresh sample following the related procedure if both values are desired.

  • Page 52: Chlorine, Total

    8.9. CHLORINE, TOTAL SPECIFICATIONS Range 0.00 to 5.00 mg/L (as Cl Resolution 0.01 mg/L Accuracy ±0.03 mg/L ±3% of reading at 25 °C Light Source LED with narrow band interference filter @ 525 nm Method Adaptation of the EPA DPD method 330.5. REQUIRED REAGENTS POWDER: Code...
  • Page 53 • Press the Zero key. The display will show “‑0.0‑” when the meter is zeroed and ready for measurement. • Remove the cuvette. POWDER REAGENT PROCEDURE • Add 1 packet of HI93711‑0 Total Chlorine Reagent. Replace the cap and shake gently for 20 seconds. •...
  • Page 54 • Add 10 mL of unreacted sample (up to the mark). Replace the cap and shake gently. 10 mL • Insert the cuvette into the instrument and close the lid. • Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 2 minutes and 30 seconds and press Read.

  • Page 55: Copper Low Range

    8.10. COPPER LOW RANGE SPECIFICATIONS Range 0.000 to 1.500 mg/L (as Cu) Resolution 0.001 mg/L Accuracy ±0.010 mg/L ±5% of reading at 25 °C Light Source LED with narrow band interference filter @ 575 nm Method Adaptation of the EPA method. REQUIRED REAGENTS Code Description...
  • Page 56 • Remove the cuvette. • Add one packet of HI95747‑0 Copper Low Range Reagent. Replace the cap and shake gently for about 15 seconds. • Reinsert the cuvette into the instrument and close the lid. • Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 45 seconds and press Read.

  • Page 57: Copper High Range

    8.11. COPPER HIGH RANGE SPECIFICATIONS Range 0.00 to 5.00 mg/L (as Cu) Resolution 0.01 mg/L Accuracy ±0.02 mg/L ±4% of reading at 25 °C Light Source LED with narrow band interference filter @ 575 nm Method Adaptation of the EPA method. REQUIRED REAGENTS Code Description...
  • Page 58 • Remove the cuvette. • Add one packet of HI93702‑0 Copper High Range Reagent. Replace the cap and shake gently for about 15 seconds. • Reinsert the cuvette into the instrument and close the lid. • Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 45 seconds and press Read.

  • Page 59: Nitrate

    8.12. NITRATE SPECIFICATIONS Range 0.0 to 30.0 mg/L (as NO ‑ N) ‑ Resolution 0.1 mg/L Accuracy ±0.5 mg/L ±10% of reading at 25 °C Light Source LED with narrow band interference filter @ 525 nm Method Adaptation of the cadmium reduction method. REQUIRED REAGENTS Code Description...
  • Page 60 • Remove the cuvette and add one packet of HI93728‑0 Nitrate Reagent. • Replace the cap and shake vigorously up and down for exactly 10 seconds. Continue to mix by inverting the cuvette gently for 50 seconds, while taking care not to induce air bubbles. Powder will not completely dissolve.
  • Page 61 • Press the Chem Frm key to convert the result to mg/L of nitrate (NO ¯). •Press  or  to return to the measurement screen. INTERFERENCES Interference may be caused by: Ammonia and amines, as urea and primary aliphatic amines Chloride above 100 ppm Chlorine above 2 ppm Copper...

  • Page 62: Nitrite, Marine Ultra Low Range

    8.13. NITRITE, MARINE ULTRA LOW RANGE SPECIFICATIONS Range 0 to 200 µg/L (as N0 ‑N) ‑ Resolution 1 µg/L Accuracy ±10 µg/L ±4% of reading at 25 °C Light Source LED with narrow band interference filter @ 466 nm Method Adaptation of the EPA Diazotization method 354.1.
  • Page 63 • Reinsert the cuvette into the instrument and close the lid. • Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 15 minutes and press Read. When the timer ends the meter will perform the reading. The instrument displays concentration in µg/L of nitrite-nitrogen (NO -N).

  • Page 64: Nitrite Low Range

    8.14. NITRITE LOW RANGE SPECIFICATIONS Range 0 to 600 µg/L (as NO ‑N) ‑ Resolution 1 µg/L Accuracy ±20 µg/L ±4% of reading at 25 °C Light Source LED with narrow band interference filter @ 466 nm Method Adaptation of the EPA Diazotization method 354.1. REQUIRED REAGENTS Code Description...
  • Page 65 • Add one packet of HI93707‑0 Nitrite Low Range Reagent. Replace the cap and shake gently for about 15 seconds. • Reinsert the cuvette into the instrument and close the lid. • Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 15 minutes and press Read.
  • Page 66 INTERFERENCES Interference may be caused by the following ions: ferrous, ferric, cupric, mercurous, silver, antimonious, bismuth, auric, lead, metavanadate and chloroplatinate. Strongly reducing and oxidizing reagents. High levels of nitrate (above 100 mg/L) could yield falsely high readings due to a minute amount of reduction to nitrite that could occur at these levels.

  • Page 67: Nitrite High Range

    8.15. NITRITE HIGH RANGE SPECIFICATIONS Range 0 to 150 mg/L (as NO ‑ Resolution 1 mg/L Accuracy ±4 mg/L ±4% of reading at 25 °C Light Source LED with narrow band interference filter @ 575 nm Method Adaptation of the Ferrous Sulfate method. REQUIRED REAGENTS Code Description...
  • Page 68 • Add one packet of HI93708‑0 Nitrite High Range Reagent. Replace the cap and shake gently until completely dissolved. • Reinsert the cuvette into the instrument and close the lid. • Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 10 minutes and press Read.

  • Page 69: Oxygen, Dissolved

    8.16. OXYGEN, DISSOLVED SPECIFICATIONS Range 0.0 to 10.0 mg/L (as O Resolution 0.1 mg/L Accuracy ±0.4 mg/L ±3% of reading at 25 °C Light Source LED with narrow band interference filter @ 420 nm Method Adaptation of the Standard Methods for the Examination of Water and Wastewater, 18th edition, Azide modified Winkler method.
  • Page 70 • Let the sample stand for approximately 2 minutes to allow flocculating agent to settle. • When the upper half of the bottle is clear, add 10 drops of HI93732C‑0 Dissolved Oxygen Reagent C. • Replace the cap and invert the bottle until the settled flocculating agent dissolves completely.
  • Page 71 • Insert the cuvette into the holder and close the lid. • Press Read to start the reading. The instrument will display the results in mg/L of oxygen (O INTERFERENCES Interferences may be caused by reducing and oxidizing materials.
  • Page 72 8.17. pH SPECIFICATIONS Range 6.5 to 8.5 pH Resolution 0.1 pH Accuracy ±0.1 pH at 25 °C Light Source LED with narrow band interference filter @ 525 nm Method Adaptation of the Phenol Red method. REQUIRED REAGENTS Code Description Quantity HI 93710‑0 pH Reagent 5 drops...
  • Page 73 • Remove the cuvette and add 5 drops of HI93710‑0 pH Reagent Indicator. Replace the cap and mix the solution. • Reinsert the cuvette into the instrument and close the lid. • Press the Read key to start the reading. The instrument displays the result in pH.

  • Page 74: Phosphate, Marine Ultra Low Range

    8.18. PHOSPHATE, MARINE ULTRA LOW RANGE SPECIFICATIONS Range 0 to 200 µg/L (as P) Resolution 1 µg/L Accuracy ±5 µg/L ±5% of reading at 25 °C Light Source LED with narrow band interference filter @ 610 nm Method Adaptation of the Standard Methods for the Examination of Water and Wastewater, 20th edition, Ascorbic Acid method.
  • Page 75 • Reinsert the cuvette into the instrument and close the lid. • Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 3 minutes and press Read. When the timer ends the meter will perform the reading. The instrument displays concentration in µg/L of phosphorus (P).

  • Page 76: Phosphate Low Range

    8.19. PHOSPHATE LOW RANGE SPECIFICATIONS Range 0.00 to 2.50 mg/L (as PO 3‑ Resolution 0.01 mg/L Accuracy ±0.04 mg/L ±4% of reading at 25 °C Light Source LED with narrow band interference filter @ 610 nm Method Adaptation of the Ascorbic Acid method. REQUIRED REAGENTS Code Description...
  • Page 77 • Reinsert the cuvette into the instrument and close the lid. • Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 3 minutes and press Read. When the timer ends the meter will perform the reading. The instrument displays concentration in mg/L of phosphate (PO ¯).

  • Page 78: Phosphate High Range

    8.20. PHOSPHATE HIGH RANGE SPECIFICATIONS Range 0.0 to 30.0 mg/L (as PO 3‑ Resolution 0.1 mg/L Accuracy ±1.0 mg/L ±4% of reading at 25 °C Light Source LED with narrow band interference filter @ 525 nm Method Adaptation of the Standard Methods for the Examination of Water and Wastewater, 18th edition, Amino Acid method.
  • Page 79 • Add one packet of HI93717B‑0 Phosphate HR Reagent B to the cuvette. Replace the cap and shake gently until completely dissolved. • Reinsert the cuvette into the instrument and close the lid. • Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 5 minutes and press Read.

  • Page 80: Error Descriptions

    9. ERROR DESCRIPTIONS The instrument shows clear warning messages when erroneous conditions appear and when measured values are outside the expected range. These messages are described below. No Light: The light source is not functioning properly. Light Leak: There is an excess amount of ambient light reaching the detector.

  • Page 81: Standard Methods

    10. STANDARD METHODS Description Range Method Alkalinity 0 to 500 mg/L Colorimetric Alkalinity, Marine 0 to 300 mg/L Colorimetric Ammonia LR 0.00 to 3.00 mg/L Nessler Ammonia MR 0.00 to 10.00 mg/L Nessler Ammonia HR 0.0 to 100.0 mg/L Nessler Calcium 0 to 400 mg/L Oxalate...

  • Page 82: Accessories

    11. ACCESSORIES 11.1. REAGENT SETS Code Description HI736‑25 25 phosphate marine ULR tests HI755‑26 25 alkalinity marine tests HI758‑26 25 calcium marine tests HI764‑25 25 nitrite marine ULR tests HI775‑26 25 alkalinity fresh water tests HI93700‑01 100 ammonia LR tests HI93700‑03 300 ammonia LR tests HI93701‑01...
  • Page 83 Code Description HI93717‑01 100 phosphate HR tests HI93717‑03 300 phosphate HR tests HI93728‑01 100 nitrate tests HI93728‑03 300 nitrate tests HI93732‑01 100 dissolved oxygen tests HI93732‑03 300 dissolved oxygen tests HI93733‑01 100 ammonia HR tests HI93733‑03 300 ammonia HR tests HI937521‑01 50 calcium fresh water tests HI937521‑03...

  • Page 84: Ph Electrodes

    11.2 pH ELECTRODES Code Description HI10530 Triple ceramic, double junction, low temperature glass, refillable pH electrode with conical tip and temperature sensor HI10430 Triple ceramic, double junction, high temperature glass, refillable pH electrode with temperature sensor HI11310 Glass body, double junction, refillable pH/temperature electrode HI11311 Glass body, double junction, refillable pH/temperature electrode with enhanced diagnostics...

  • Page 85: Ph Solutions

    11.3 pH SOLUTIONS BUFFER SOLUTIONS Code Description HI70004P pH 4.01 Buffer Sachets, 20 mL (25 pcs.) HI70007P pH 7.01 Buffer Sachets, 20 mL (25 pcs.) HI70010P pH 10.01 Buffer Sachets, 20 mL (25 pcs.) HI7001L pH 1.68 Buffer Solution, 500 mL HI7004L pH 4.01 Buffer Solution, 500 mL HI7006L...
  • Page 86 ELECTRODE CLEANING SOLUTIONS Code Description HI70000P Electrode Rinse Sachets, 20 mL (25 pcs.) HI7061L General Cleaning Solution, 500 mL HI7073L Protein Cleaning Solution, 500 mL HI7074L Inorganic Cleaning Solution, 500 mL HI7077L Oil & Fat Cleaning Solution, 500 mL HI8061L General Cleaning Solution in FDA approved bottle, 500 mL HI8073L Protein Cleaning Solution in FDA approved bottle, 500 mL...

  • Page 87: Other Accessories

    11.4. OTHER ACCESSORIES Code Description HI72083300 carrying case HI731318 cloth for wiping cuvettes (4 pcs.) HI731331 glass cuvettes (4 pcs.) HI731335N cap for cuvette (4 pcs.) HI731340 200 µL automatic pipette HI731341 1000 µL automatic pipette HI731342 2000 µL automatic pipette HI740034P cap for 100 mL beaker (10 pcs.) HI740036P...
  • Page 88 Code Description HI83303‑11 CAL Check cuvette kit for HI83303 HI83300‑100 Sample preparation kit consisting of activated carbon for 50 tests, demineralizer bottle for 10 L of water, 100 mL graduated beaker with cap, 170 mL graduated beaker with cap, 3 mL pipette, 60 mL syringe, 5 mL syringe, graduated cylinder, spoon, funnel, filter paper (25 pcs.).

  • Page 89: Abbreviations

    12. ABBREVIATIONS EPA: US Environmental Protection Agency °C: degree Celsius °F: degree Fahrenheit µg/L: micrograms per liter (ppb) mg/L: milligrams per liter (ppm) g/L: grams per liter (ppt) milliliter good laboratory practice ultra high range ultra low range high range medium range low range PAN:...

  • Page 90: Recommendations For Users

    Recommendations Before using this product make sure that they are entirely suitable for your specific application and for the environment in which they for Users are used. Operation of these instruments may cause unacceptable interferences to other electronic equipment. Take all necessary steps to correct such interferences.

  • Page 91: Warranty

    Returned Goods Authorization (RGA) number from the Technical Service department and then send it with shipping costs prepaid. When shipping any instrument, make sure it is properly packed for complete protection. Hanna Instruments reserves the right to modify the design, construction or appearance of its products without advance notice.
  • Page 92 World Headquarters Hanna Instruments Inc. Highland Industrial Park 584 Park East Drive Woonsocket, RI 02895 USA www.hannainst.com Local Office Hanna Instruments USA 270 George Washington Highway Smithfield, RI 02917 Phone: 800.426.6287 Fax: 401.765.7575 e‑mail: tech@hannainst.com MAN83303 10/16‑1 Printed in ROMANIA...

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