Hanna Instruments HI83303 Instruction Manual
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Hanna Instruments HI83303 Instruction Manual

Multiparameter photometer
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HI83303
Multiparameter Photometer
for Aquaculture

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Summary of Contents for Hanna Instruments HI83303

  • Page 1 HI83303 Multiparameter Photometer for Aquaculture...
  • Page 2 If you need additional technical information, do not hesitate to e‑mail us at tech@hannainst.com or view our worldwide contact list at www.hannainst.com. All rights are reserved. Reproduction in whole or in part is prohibited without the written consent of the copyright owner, Hanna Instruments Inc., Woonsocket, Rhode Island, 02895, USA.

  • Page 3: Table Of Contents

    TABLE OF CONTENTS 1. PRELIMINARY EXAMINATION ....................5 2. SAFETY MEASURES ......................5 3. SPECIFICATIONS ......................... 6 4. ABBREVIATIONS ........................ 7 5. DESCRIPTION ........................8 5.1. GENERAL DESCRIPTION & INTENDED USE ..............8 5.2. PRECISION & ACCURACY .................... 8 5.3. FUNCTIONAL DESCRIPTION ..................9 5.4.
  • Page 4 10. METHOD PROCEDURES ....................31 10.1. ALKALINITY ......................31 10.2. ALKALINITY, MARINE ..................... 33 10.3. AMMONIA LOW RANGE ..................35 10.4. AMMONIA MEDIUM RANGE ................... 38 10.5. AMMONIA HIGH RANGE ..................41 10.6. CALCIUM ......................44 10.7. CALCIUM, MARINE ....................47 10.8.

  • Page 5: Preliminary Examination

    1. PRELIMINARY EXAMINATION Remove the instrument and accessories from the packaging and examine it carefully. For further assistance, please contact your local Hanna Instruments Office or email us at tech@hannainst.com. Each HI83303 is delivered in a rugged carrying case and is supplied with: •...

  • Page 6: Specifications

    3. SPECIFICATIONS 5 x optical channels Measurement Channels 1 x digital electrode channel (pH measurement) Range 0.000 to 4.000 Abs Resolution 0.001 Abs Accuracy ±0.003 Abs @ 1.000 Abs Light source Light Emitting Diode Photometer Bandpass filter bandwidth 8 nm Bandpass filter wavelength accuracy ±1.0 nm Light detector Silicon photocell...

  • Page 7: Abbreviations

    4. ABBREVIATIONS Absorbance Good Laboratory Practice NIST National Institute of Standards and Technology US Environmental Protection Agency grams per liter (ppt) µg/L micrograms per liter (ppb) mg/L milligrams per liter (ppm) milliliter High Range Low Range Medium Range Ultra Low Range...

  • Page 8: Description

    With the CAL Check feature users are able to validate the performance of the instrument and apply a user calibration (if necessary). Hanna Instruments CAL Check cuvettes are made with NIST traceable standards. Probe mode uses a digital pH probe with a one or two-point calibration.

  • Page 9: Functional Description

    5.3. FUNCTIONAL DESCRIPTION 1. Splash-proof keypad 2. Liquid Crystal Display (LCD) 3. Indexing mark 4. Protective port covers 5. Light-blocking cover panel 6. Cuvette holder 7. ON/OFF power button 8. 3.5 mm TRRS (jack) input for digital electrodes 9. Standard USB host connector for data transfer to a USB flash drive 10.

  • Page 10: Principle Of Operation

    Keypad Description The keypad contains 12 direct keys and 3 functional keys with the following functions: Press the functional key to perform the function displayed above it on the LCD. Press to access the list of photometer methods. Press to move up in a menu or a help screen, to increment a set value or to access second level functions.

  • Page 11: Optical System

    5.5. OPTICAL SYSTEM Instrument Block Diagram The internal reference system (reference detector) of the HI83303 photometer compensates for any drifts due to power fluctuations or ambient temperature changes, providing a stable source of light for your blank (zero) measurement and sample measurement.

  • Page 12: General Operations

    Photometer and Probe. Photometer mode enables on-demand measurement of a cuvette using the integrated optical system. Probe mode enables continuous measurement using a Hanna Instruments digital electrode connected to the 3.5 mm port. To switch between Photometer mode and Probe mode, use the MODE key.

  • Page 13: General Setup

    6.3. GENERAL SETUP Press the SETUP key to enter in Setup menu, highlight desired option using the  keys and press Select. CAL Check (Photometer Mode Only) Press Select to enter the CAL Check screen. The date, time and values for the last CAL Check are displayed on the screen.
  • Page 14 Date & Time Press Modify to change the date and time. Press the functional keys or the  keys to highlight the value to be modified (year, month, day, hour, minute or second). Use the  keys to change the value.
  • Page 15 Language Option: Português, Deutsch, English, Español, Français, Italiano, Dutch Press Modify to change the language. Use the   keys to select the desired language. Press Select to change the language. Beeper Option: Enable or Disable When enabled, a short beep is heard every time a key is pressed. A long beep alert sounds when the pressed key is not active or an error is detected.

  • Page 16: Contextual Help

    ESC key to return to the Setup menu. 6.4. CONTEXTUAL HELP HI83303 offers an interactive contextual help mode that assists the user at any time. To access the help screen press the HELP key. The instrument will display additional information related to the current screen.

  • Page 17: Logging Data & Data Management

    7. LOGGING DATA & DATA MANAGEMENT The instrument features a data log function to help the user to keep track of measurements. The data log can hold 1000 individual measurements. Storing, viewing and deleting the data is possible using the LOG and RECALL keys. 7.1.

  • Page 18: Data Management

    Windows Explorer or Mac Finder) to move the file from the meter to the PC. Log data is exported as a single file (HI83303.csv) containing all logged photometer and probe data. The CSV file may be opened with a text editor or spreadsheet application.

  • Page 19: Photometer Mode

    8. PHOTOMETER MODE 8.1. METHOD SELECTION In order to select the desired method press the METHOD key and a screen with the available methods will appear. Press the  keys to highlight the desired method. Press Select. After the desired method is selected, follow the procedure described in the related section. Before performing a method, read all the instructions carefully.

  • Page 20: Cuvette Preparation

    Proper Use of Dropper Bottle 1. Tap the dropper on the table several times. 2. Remove the cap and wipe the outside of the tip with a cloth. 3. Keep the dropper bottle in a vertical position while dosing the reagent. Proper Use of Powder Packet 1.
  • Page 21 (b) Shaking the cuvette, moving the cuvette up and down. The movement may be gentle or vigorous. This mixing technique is indicated with “shake gently” or “shake vigorously”, and one of the following icons: shake gently shake vigorously (c) Swirl the cuvette gently to mix the solution. This mixing technique is indicated with one of the following icons: In order to avoid reagent leaking and to obtain more accurate measurements, close the cuvette first with the supplied High-Density Polyethylene (HDPE)

  • Page 22: Timers & Measurement Functions

    Discard the sample immediately after the reading is taken, or the glass might become permanently stained. All the reaction times reported in this manual are at 25 °C (77 °F). In general, the reaction time should be increased for temperatures lower than 20 °C (68 °F) and decreased for temperatures higher than 25 °C (77 °F).

  • Page 23: Meter Validation & Cal Check

    8.6. METER VALIDATION & CAL CHECK ® Warning: Do not validate the meter with standard solutions other than the Hanna Instruments CAL Check Standards. For accurate validation results, please perform tests at room temperature, 18 to 25 ° C (64.4 to 77.0 ° F).

  • Page 24: Absorbance Measurements

    4. Insert a cuvette filled with deionized water, then press Zero. 5. Insert the prepared sample cuvette, then press Read. ® Warning: Never use absorbance methods for validation using Hanna Instruments CAL Check cuvettes. The factory calibration corrections for CAL Check cuvettes are applied while in CAL Check...

  • Page 25: Probe Mode

    ® HI83303 can be used to perform direct pH measurements by connecting a Hanna Instruments digital pH electrode with a 3.5 mm TRRS connector. Connect the electrode to the 3.5 mm port marked with EXT PROBE located at the rear of the meter. If the meter is in Photometer mode, set the meter to Probe mode by pressing the MODE key.

  • Page 26: Ph Calibration

    9.2. pH CALIBRATION From the probe measurement screen, press Calibrate to begin the calibration process. During pH calibration, the display will show the current pH reading, temperature reading, selected buffer type and the buffer number (“Buffer: 1” for the first buffer, “Buffer: 2” for the second buffer). Press Clear to clear the current calibration.

  • Page 27: Ph Messages & Warnings

    9.3. pH MESSAGES & WARNINGS No Probe No probe is connected or the probe is broken. Connecting The meter has detected a probe and is reading the probe configuration and calibration information. Incompatible Probe The connected probe is not compatible with this device. Incompatible Calibration The probe’s current calibration is not compatible with this meter.

  • Page 28: Ph Glp

    Check Probe & Buffer There is a large difference between the pH measurement and the selected buffer value or the electrode slope is outside of the accepted slope limit. Clean the probe and confirm the correct buffer selection. Wrong Temperature The buffer temperature is outside of the acceptable window for the selected buffer value.

  • Page 29: Ph Electrode Conditioning & Maintenance

    9.5. pH ELECTRODE CONDITIONING & MAINTENANCE Remove the protective cap. Do not be alarmed if salt deposits are present, this is normal. Rinse the probe with water. Shake the electrode alike a clinical thermometer to eliminate any air bubbles inside the glass bulb. If the bulb and / or junction are dry, soak the electrode in HI70300 HI80300...
  • Page 30 Periodic Maintenance Inspect the electrode and the cable. The cable used for connection to the instrument must be intact and there must be no points of broken insulation on the cable, connectors must be perfectly clean and dry. If there are any scratches or cracks on the electrode stem or bulb, replace the electrode. For refillable electrodes, refill the reference chamber with fresh electrolyte (HI7082 HI8082...

  • Page 31: Method Procedures

    10. METHOD PROCEDURES 10.1. ALKALINITY SPECIFICATIONS Range 0 to 500 mg/L (as CaCO Resolution 1 mg/L Accuracy ±5 mg/L ±5% of reading at 25 °C Light Source LED with narrow band interference filter @ 610 nm Method Colorimetric Method REQUIRED REAGENTS Code Description Quantity...
  • Page 32 • Remove the cuvette. • Add 1mL of HI775S Alkalinity Reagent to the sample using a 1 mL syringe. 1 mL • Replace the plastic stopper and the cap. Invert 5 times. • Insert the cuvette into the holder and close the lid. •...

  • Page 33: Alkalinity, Marine

    10.2. ALKALINITY, MARINE SPECIFICATIONS Range 0 to 300 mg/L (as CaCO Resolution 1 mg/L Accuracy ±5 mg/L ±5% of reading at 25 °C Light Source LED with narrow band interference filter @ 610 nm Method Colorimetric Method REQUIRED REAGENTS Code Description Quantity HI755S...
  • Page 34 • Remove the cuvette. 1 mL • Add 1 mL of HI755S Alkalinity Reagent to the sample using a 1mL syringe. • Replace the plastic stopper and the cap. Invert 5 times. • Insert the cuvette into the holder and close the lid. •...

  • Page 35: Ammonia Low Range

    10.3. AMMONIA LOW RANGE SPECIFICATIONS Range 0.00 to 3.00 mg/L (as NH Resolution 0.01 mg/L Accuracy ±0.04 mg/L ±4% of reading at 25 °C Light Source LED with narrow band interference filter @ 420 nm Method Adaptation of the ASTM Manual of Water and Environmental Technology, D1426 Nessler Method REQUIRED REAGENTS Code...
  • Page 36 • Remove the cuvette. • Add 4 drops of HI93700A-0 Ammonia Low Range Reagent ×4 A. Replace the plastic stopper and the cap. Swirl to mix the solution. • Add 4 drops of HI93700B-0 Ammonia Low Range Reagent ×4 B. Replace the plastic stopper and the cap. Swirl to mix the solution.
  • Page 37 • Press Chem Frm to convert the result to mg/L of ammonia (NH ) and ammonium (NH • Press the  or  key to return to the measurement screen. INTERFERENCES Interference may be caused by: • Hardness above 1 g/L •...

  • Page 38: Ammonia Medium Range

    10.4. AMMONIA MEDIUM RANGE SPECIFICATIONS Range 0.00 to 10.00 mg/L (as NH Resolution 0.01 mg/L Accuracy ±0.05 mg/L ±5% of reading at 25 °C Light Source LED with narrow band interference filter @ 420 nm Method Adaptation of the ASTM Manual of Water and Environmental Technology, D1426, Nessler Method REQUIRED REAGENTS Code...
  • Page 39 • Remove the cuvette. ×4 • Add 4 drops of HI93715A-0 Ammonia Medium Range Reagent A. Replace the plastic stopper and the cap. Swirl to mix the solution. • Add 4 drops of HI93715B-0 Ammonia Medium Range Reagent B. ×4 Replace the plastic stopper and the cap.
  • Page 40 • Press Chem Frm to convert the result to mg/L of ammonia (NH ) and ammonium (NH • Press the  or  key to return to the measurement screen. INTERFERENCES Interference may be caused by: • Hardness above 1 g/L •...

  • Page 41: Ammonia High Range

    10.5. AMMONIA HIGH RANGE SPECIFICATIONS Range 0.0 to 100.0 mg/L (as NH Resolution 0.1 mg/L Accuracy ±0.5 mg/L ±5% of reading at 25 °C Light Source LED with narrow band interference filter @ 420 nm Method Adaptation of the ASTM Manual of Water and Environmental Technology, D1426, Nessler Method REQUIRED REAGENTS Code...
  • Page 42 • Press Zero. The display will show “-0.0-” when the meter is zeroed and ready for measurement. • Remove the cuvette. • Add 4 drops of HI93733A-0 Ammonia High Range Reagent A. ×4 Replace the plastic stopper and the cap. Swirl to mix the solution. •...
  • Page 43 • Press Chem Frm to convert the result to mg/L of ammonia (NH ) and ammonium (NH • Press the  or  key to return to the measurement screen. INTERFERENCES Interference may be caused by: • Hardness above 1 g/L •...

  • Page 44: Calcium

    10.6. CALCIUM SPECIFICATIONS Range 0 to 400 mg/L (as Ca Resolution 1 mg/L Accuracy ±10 mg/L ±5% of reading at 25 °C Light Source LED with narrow band interference filter @ 466 nm Method Adaptation of the Oxalate Method REQUIRED REAGENTS Code Description Quantity...
  • Page 45 • Replace the plastic stopper and the cap. Invert several times to mix. • Insert the cuvette into the holder and close the lid. • Press Zero. The display will show “-0.0-” when the meter is zeroed and ready for measurement. •...
  • Page 46 • After waiting 5 minutes, invert the cuvette 10 times to mix (about 15 seconds). • Insert the cuvette into the holder and close the lid. • Press Read to start the reading. The instrument displays the results in mg/L of calcium (Ca INTERFERENCES Interference may be caused by: •...

  • Page 47: Calcium, Marine

    10.7. CALCIUM, MARINE SPECIFICATIONS Range 200 to 600 mg/L (as Ca Resolution 1 mg/L Accuracy ±6% of reading at 25 °C Light Source LED with narrow band interference filter @ 610 nm Method Adaptation of the Zincon Method REQUIRED REAGENTS Code Description Quantity...
  • Page 48 • Press Zero. The display will show “-0.0-” when the meter is zeroed and ready for measurement. • Remove the cuvette. • Use the minipipette to add 0.1 mL of sample to the cuvette. 0.1 mL • Add one packet of HI7582 Calcium Reagent B.

  • Page 49: Chlorine, Free

    10.8. CHLORINE, FREE SPECIFICATIONS Range 0.00 to 5.00 mg/L (as Cl Resolution 0.01 mg/L Accuracy ±0.03 mg/L ±3% of reading at 25 °C Light Source LED with narrow band interference filter @ 525 nm Method Adaptation of the EPA DPD Method 330.5 REQUIRED REAGENTS POWDER Code...
  • Page 50 • Press Zero. The display will show “-0.0-” when the meter is zeroed and ready for measurement. • Remove the cuvette. POWDER REAGENT PROCEDURE • Add the content of one packet of HI93701-0 Free Chlorine Reagent. Replace the plastic stopper and the cap.
  • Page 51 LIQUID REAGENT PROCEDURE • To an empty cuvette add 3 drops of HI93701A-F Free Chlorine Reagent A and 3 drops of HI93701B-F Free Chlorine Reagent B. ×3 ×3 • Replace the plastic stopper and the cap. Swirl gently to mix. •...

  • Page 52: Chlorine, Total

    10.9. CHLORINE, TOTAL SPECIFICATIONS Range 0.00 to 5.00 mg/L (as Cl Resolution 0.01 mg/L Accuracy ±0.03 mg/L ±3% of reading at 25 °C Light Source LED with narrow band interference filter @ 525 nm Method Adaptation of the EPA DPD Method 330.5 REQUIRED REAGENTS POWDER Code...
  • Page 53 • Press Zero. The display will show “-0.0-” when the meter is zeroed and ready for measurement. • Remove the cuvette. POWDER REAGENT PROCEDURE • Add 1 packet of HI93711-0 Total Chlorine Reagent. Replace the plastic stopper and the cap. Shake gently for 20 seconds.
  • Page 54 LIQUID REAGENT PROCEDURE • To an empty cuvette add 3 drops of HI93701A-T Total Chlorine Reagent A, 3 drops of HI93701B-T ×3 ×3 ×1 Total Chlorine Reagent B and 1 drop of HI93701C-T Total Chlorine Reagent C. • Replace the plastic stopper and the cap. Swirl gently to mix.
  • Page 55 INTERFERENCES Interference may be caused by: • Bromine, Iodine, Oxidized forms of Chromium and Manganese, Ozone • Hardness greater than 500 mg/L CaCO , to remove the interference shake the sample for approximately 2 minutes after adding the powder reagent •...

  • Page 56: Copper Low Range

    10.10. COPPER LOW RANGE SPECIFICATIONS Range 0.000 to 1.500 mg/L (as Cu Resolution 0.001 mg/L Accuracy ±0.010 mg/L ±5% of reading at 25 °C Light Source LED with narrow band interference filter @ 575 nm Method Adaptation of the EPA Method REQUIRED REAGENTS Code Description...
  • Page 57 • Remove the cuvette. • Add one packet of HI95747-0 Copper Low Range Reagent. Replace the plastic stopper and the cap. Shake gently for about 15 seconds. • Insert the cuvette into the holder and close the lid. • Press Timer and the display will show the countdown prior to the measurement or wait 45 seconds and press Read.

  • Page 58: Copper High Range

    10.11. COPPER HIGH RANGE SPECIFICATIONS Range 0.00 to 5.00 mg/L (as Cu Resolution 0.01 mg/L Accuracy ±0.02 mg/L ±4% of reading at 25 °C Light Source LED with narrow band interference filter @ 575 nm Method Adaptation of the EPA Method REQUIRED REAGENTS Code Description...
  • Page 59 • Remove the cuvette. • Add one packet of HI93702-0 Copper High Range Reagent. Replace the plastic stopper and the cap. Shake gently for about 15 seconds. • Insert the cuvette into the holder and close the lid. • Press Timer and the display will show the countdown prior to the measurement or wait 45 seconds and press Read.

  • Page 60: Nitrate

    10.12. NITRATE SPECIFICATIONS Range 0.0 to 30.0 mg/L (as NO - N) Resolution 0.1 mg/L Accuracy ±0.5 mg/L ±10% of reading at 25 °C Light Source LED with narrow band interference filter @ 525 nm Method Adaptation of the Cadmium Reduction Method REQUIRED REAGENTS Code Description...
  • Page 61 • Remove the cuvette and add one packet of HI93728-0 Nitrate Reagent. • Replace the plastic stopper and the cap. Shake vigorously up and down for exactly 10 seconds. Continue to mix by inverting the cuvette gently for 50 seconds, while taking care not to induce air bubbles.
  • Page 62 • Press Chem Frm to convert the result to mg/L of nitrate (NO ¯). • Press the  or  key to return to the measurement screen. INTERFERENCES Interference may be caused by: • Ammonia and amines, as urea and primary aliphatic amines •...

  • Page 63: Nitrite, Marine Ultra Low Range

    10.13. NITRITE, MARINE ULTRA LOW RANGE SPECIFICATIONS Range 0 to 200 µg/L (as N0 Resolution 1 µg/L Accuracy ±10 µg/L ±4% of reading at 25 °C Light Source LED with narrow band interference filter @ 466 nm Method Adaptation of the EPA Diazotization Method 354.1 REQUIRED REAGENTS Code Description...
  • Page 64 • Remove the cuvette. • Add one packet of HI764-0 Nitrite Ultra Low Range Reagent. Replace the plastic stopper and the cap. Shake gently for about 15 seconds. • Insert the cuvette into the holder and close the lid. • Press Timer and the display will show the countdown prior to the measurement or wait 15 minutes and press Read.

  • Page 65: Nitrite Low Range

    10.14. NITRITE LOW RANGE SPECIFICATIONS Range 0 to 600 µg/L (as NO Resolution 1 µg/L Accuracy ±20 µg/L ±4% of reading at 25 °C Light Source LED with narrow band interference filter @ 466 nm Method Adaptation of the EPA Diazotization Method 354.1 REQUIRED REAGENTS Code Description...
  • Page 66 • Remove the cuvette. • Add one packet of HI93707-0 Nitrite Low Range Reagent. • Replace the plastic stopper and the cap. Shake gently for about 15 seconds. • Insert the cuvette into the holder and close the lid. • Press Timer and the display will show the countdown prior to the measurement or wait 15 minutes and press Read.

  • Page 67: Nitrite High Range

    10.15. NITRITE HIGH RANGE SPECIFICATIONS Range 0 to 150 mg/L (as NO Resolution 1 mg/L Accuracy ±4 mg/L ±4% of reading at 25 °C Light Source LED with narrow band interference filter @ 575 nm Method Adaptation of the Ferrous Sulfate Method REQUIRED REAGENTS Code Description...
  • Page 68 • Remove the cuvette. • Add one packet of HI93708-0 Nitrite High Range Reagent. Replace the plastic stopper and the cap. Shake gently until completely dissolved. • Insert the cuvette into the holder and close the lid. • Press Timer and the display will show the countdown prior to the measurement or wait 10 minutes and press Read.

  • Page 69: Oxygen, Dissolved

    10.16. OXYGEN, DISSOLVED SPECIFICATIONS Range 0.0 to 10.0 mg/L (as O Resolution 0.1 mg/L Accuracy ±0.4 mg/L ±3% of reading at 25 °C Light Source LED with narrow band interference filter @ 420 nm Method Adaptation of Standard Methods for the Examination of Water and Wastewater, 18 Edition, Azide Modified Winkler Method REQUIRED REAGENTS...
  • Page 70 • Let the sample stand for approximately 2 minutes to allow flocculating agent to settle. ×10 • When the upper half of the bottle is clear, add 10 drops of HI93732C-0 Dissolved Oxygen Reagent C. • Replace the cap and invert the bottle until the settled flocculating agent dissolves completely.
  • Page 71 • Insert the cuvette into the holder and close the lid. • Press Read to start the reading. The instrument will display the results in mg/L of oxygen (O INTERFERENCES Interference may be caused by: • Reducing and oxidizing materials...
  • Page 72 10.17. pH SPECIFICATIONS Range 6.5 to 8.5 pH Resolution 0.1 pH Accuracy ±0.1 pH at 25 °C Light Source LED with narrow band interference filter @ 525 nm Method Adaptation of the Phenol Red Method REQUIRED REAGENTS Code Description Quantity HI93710-0 pH Reagent 5 drops...
  • Page 73 • Remove the cuvette and add 5 drops of HI93710-0 ×5 Reagent Indicator. Replace the plastic stopper and the cap and mix the solution. • Insert the cuvette into the holder and close the lid. • Press Read to start the reading. The instrument displays the result in pH.

  • Page 74: Phosphate, Marine Ultra Low Range

    10.18. PHOSPHATE, MARINE ULTRA LOW RANGE SPECIFICATIONS Range 0 to 200 µg/L (as P) Resolution 1 µg/L Accuracy ±5 µg/L ±5% of reading at 25 °C Light Source LED with narrow band interference filter @ 610 nm Method Adaptation of Standard Methods for the Examination of Water and Wastewater, 20 Edition, Ascorbic Acid Method REQUIRED REAGENTS...
  • Page 75 • Insert the cuvette into the holder and close the lid. • Press Timer and the display will show the countdown prior to the measurement or wait 3 minutes and press Read. When the timer ends the meter will perform the reading. The instrument displays concentration in µg/L of phosphorus (P).

  • Page 76: Phosphate Low Range

    10.19. PHOSPHATE LOW RANGE SPECIFICATIONS Range 0.00 to 2.50 mg/L (as PO Resolution 0.01 mg/L Accuracy ±0.04 mg/L ±4% of reading at 25 °C Light Source LED with narrow band interference filter @ 610 nm Method Adaptation of the Ascorbic Acid Method REQUIRED REAGENTS Code Description...
  • Page 77 • Insert the cuvette into the holder and close the lid. • Press Timer and the display will show the countdown prior to the measurement or wait 3 minutes and press Read. When the timer ends the meter will perform the reading. The instrument displays concentration in mg/L of phosphate (PO ¯).

  • Page 78: Phosphate High Range

    10.20. PHOSPHATE HIGH RANGE SPECIFICATIONS Range 0.0 to 30.0 mg/L (as PO Resolution 0.1 mg/L Accuracy ±1.0 mg/L ±4% of reading at 25 °C Light Source LED with narrow band interference filter @ 525 nm Method Adaptation of Standard Methods for the Examination of Water and Wastewater, 18 Edition, Amino Acid Method REQUIRED REAGENTS...
  • Page 79 • Add 10 drops of HI93717A-0 Phosphate HR Reagent A. ×10 • Add one packet of HI93717B-0 Phosphate HR Reagent B to the cuvette. Replace the plastic stopper and the cap. Shake gently until completely dissolved. • Insert the cuvette into the holder and close the lid. •...
  • Page 80 • Press the  or  key to return to the measurement screen. INTERFERENCES Interference may be caused by: • Sulfide • Chloride above 150000 mg/L • Magnesium above 40000 mg/L CaCO • Calcium above 10000 mg/L CaCO • Iron (Ferrous) above 100 mg/L...

  • Page 81: Warnings & Errors

    There is an excess amount of ambient light reaching the detector. Make sure the lid is closed before performing any measurements. If the issue persists, please contact Hanna Instruments technical support. The sample and the zero cuvettes are inverted. Swap the cuvettes and repeat the measurement.
  • Page 82 The measured value cannot be calculated. Please check sample preparation and measurement procedure. Stored results of the CAL Check measurements have been lost. Please redo the CAL Check measurements to ensure accurate results. User settings have been lost. Please reset the values. If the issue persists, please contact Hanna Instruments technical support.
  • Page 83 Real time clock is not accurate. Some features are no longer available. Restart the meter. If the issue persists, please contact Hanna Instruments technical support. The device serial number can not be identified. Some features are no longer available. Restart the meter. If the issue persists, please contact Hanna Instruments technical support.

  • Page 84: Standard Methods

    12. STANDARD METHODS Description Range Method Alkalinity 0 to 500 mg/L (as CaCO Colorimetric Alkalinity, Marine 0 to 300 mg/L (as CaCO Colorimetric Ammonia LR 0.00 to 3.00 mg/L (as NH Nessler Ammonia MR 0.00 to 10.00 mg/L (as NH Nessler Ammonia HR 0.0 to 100.0 mg/L (as NH...

  • Page 85: Accessories

    13. ACCESSORIES 13.1. REAGENT SETS Code Description HI736-25 25 phosphate marine ULR tests HI755-26 25 alkalinity marine tests HI758-26 25 calcium marine tests HI764-25 25 nitrite marine ULR tests HI775-26 25 alkalinity fresh water tests HI93700-01 100 ammonia LR tests HI93700-03 300 ammonia LR tests HI93701-01...
  • Page 86 Code Description HI93715-03 300 ammonia MR tests HI93717-01 100 phosphate HR tests HI93717-03 300 phosphate HR tests HI93728-01 100 nitrate tests HI93728-03 300 nitrate tests HI93732-01 100 dissolved oxygen tests HI93732-03 300 dissolved oxygen tests HI93733-01 100 ammonia HR tests HI93733-03 300 ammonia HR tests HI937521-01...

  • Page 87: Ph Electrodes

    13.2. pH ELECTRODES Code Description Triple ceramic, double junction, low temperature glass, refillable pH HI10530 electrode with conical tip and temperature sensor Triple ceramic, double junction, high temperature glass, refillable pH HI10430 electrode with temperature sensor HI11310 Glass body, double junction, refillable pH/temperature electrode Glass body, double junction, refillable pH/temperature electrode with HI11311 enhanced diagnostics...

  • Page 88: Ph Solutions

    13.3. pH SOLUTIONS BUFFER SOLUTIONS Code Description HI70004P pH 4.01 buffer sachet, 20 mL (25 pcs.) HI70007P pH 7.01 buffer sachet, 20 mL (25 pcs.) pH 10.01 buffer sachet, 20 mL (25 pcs.) HI70010P HI7001L pH 1.68 buffer solution, 500 mL HI7004L pH 4.01 buffer solution, 500 mL HI7006L...
  • Page 89 ELECTRODE CLEANING SOLUTIONS Code Description HI70000P Electrode rinse sachet, 20 mL (25 pcs.) HI7061L General cleaning solution, 500 mL HI7073L Protein cleaning solution, 500 mL HI7074L Inorganic cleaning solution, 500 mL HI7077L Oil & fat cleaning solution, 500 mL HI8061L General cleaning solution in FDA approved bottle, 500 mL HI8073L Protein cleaning solution in FDA approved bottle, 500 mL...

  • Page 90: Other Accessories

    13.4. OTHER ACCESSORIES Code Description HI72083300 carrying case HI731318 cloth for wiping cuvettes (4 pcs.) HI731331 glass cuvette (4 pcs.) HI731335N cap for cuvette (4 pcs.) HI731340 200 µL automatic pipette HI731341 1000 µL automatic pipette HI731342 2000 µL automatic pipette HI740034P cap for 100 mL beaker (10 pcs.) HI740036P...
  • Page 91 Code Description HI83303-11 CAL Check cuvette kit for HI83303 Sample preparation kit consisting of activated carbon for 50 tests, demineralizer bottle for 10 L of water, 100 mL graduated beaker with cap, HI83300-100 170 mL graduated beaker with cap, 3 mL pipette, 60 mL syringe, 5 mL syringe, graduated cylinder, spoon, funnel, filtter paper (25 pcs.)

  • Page 92: Certification

    CERTIFICATION All Hanna Instruments conform to the CE European Directives. Disposal of Electrical & Electronic Equipment. The product should not be treated as household waste. Instead hand it over to the appropriate collection point for the recycling of electrical and electronic equipment which will conserve natural resources.

  • Page 93: Warranty

    Damage due to accidents, misuse, tampering or lack of prescribed maintenance is not covered. If service is required, contact your local Hanna Instruments Office. If under warranty, report the model number, date of purchase, serial number (engraved on the bottom of the meter) and the nature of the problem.
  • Page 94 MAN83303 01/21...
  • Page 95 Hanna Instruments reserves the right to modify the design, construction or appearance of its products without advance notice.
  • Page 96 World Headquarters Hanna Instruments Inc. Highland Industrial Park 584 Park East Drive Woonsocket, RI 02895 USA www.hannainst.com MAN83303 Printed in ROMANIA...

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