Hanna Instruments HI 83749 Instruction Manual
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Hanna Instruments HI 83749 Instruction Manual

Turbidity & bentocheck
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HI 83749
Turbidity & Bentocheck

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Summary of Contents for Hanna Instruments HI 83749

  • Page 1 HI 83749 Turbidity & Bentocheck...
  • Page 2 If you need additional technical information, do not hesitate to e‑mail us at tech@hannainst.com or view our worldwide contact list at www.hannainst.com. All rights are reserved. Reproduction in whole or in part is prohibited without the written consent of the copyright owner, Hanna Instruments Inc., Woonsocket, Rhode Island, 02895, USA.

  • Page 3: Table Of Contents

    TABLE OF CONTENTS 1. PRELIMINARY EXAMINATION ....................5 2. GENERAL DESCRIPTION ....................... 6 2.1. BENTOCHECK ......................6 2.2. TAG IDENTIFICATION SYSTEM .................. 7 2.3. ABBREVIATIONS..................... 8 3. SPECIFICATIONS ......................... 9 4. FUNCTIONAL DESCRIPTION ....................10 4.1. INSTRUMENT DESCRIPTION .................. 10 4.2.
  • Page 4 12. SETUP ........................... 35 12.1. SET REFERENCE VALUE ..................35 12.2. ACTIVATING COMPARATIVE MODE ................ 36 12.3. SET EPA COMPLIANCE MODE ................37 12.4. SET BEEPER ....................... 38 12.5. SHOW / HIDE THE TIME ..................38 12.6. SET THE DATE ....................39 12.7.

  • Page 5: Preliminary Examination

    1. PRELIMINARY EXAMINATION Remove the meter from the packing material and examine it carefully. For further assistance, please contact your local Hanna Instruments office or email us at tech@hannainst.co. This HI83749 Turbidity & Bentocheck Meter is supplied complete with: • Six sample cuvettes and caps •...

  • Page 6: General Description

    2. GENERAL DESCRIPTION HI83749 is an auto diagnostic microprocessor meter that benefits from Hanna’s years of experience as manufacturer of analytical instruments. The meter is especially designed for wine analyses and, beside turbidity measurements, it allows to make tests to verify protein stability (e.g. Bentocheck). The instrument compensates wine color to guarantee accurate readings during the vinification process;...

  • Page 7: Tag Identification System

    If protein instability is detected, a next test can help defining the right amount of bentonite to be added for improving protein stability. It is important not to overdose bentonite, avoiding stripping wine flavour, body, and significant loss of color, especially in young red wines. Moreover, adding only the necessary amount of bentonite to obtain the desired protein stability is also costs saving.

  • Page 8: Abbreviations

    2.3. ABBREVIATIONS Nephelometric Turbidity Units Formazin Turbidity Units USEPA US Environmental Protection Agency Liquid Crystal Display Real Time Clock T.I.S. Tag Identification System iButton® is registered Trademark of “MAXIM/DALLAS semiconductor Corp.” Windows® is registered Trademark of “MICROSOFT Corporation” A light beam that passes through the sample is scattered in all directions. The intensity and pattern of the scattered light is affected by many variables like wavelenght of the incident light, particle size, shape, refractive index and color.

  • Page 9: Specifications

    3. SPECIFICATIONS Range 0.00 to 9.99 NTU 10.0 to 99.9 NTU 100 to 1200 NTU Range Selection Automatically Resolution 0.01 NTU from 0.00 to 9.99 NTU 0.1 NTU from 10.0 to 99.9 NTU 1 NTU from 100 to 1200 NTU Accuracy ±2% of reading plus 0.05 NTU Repeatibility...

  • Page 10: Functional Description

    4. FUNCTIONAL DESCRIPTION 4.1. INSTRUMENT DESCRIPTION 1. Cuvette lid 2. Cuvette holder 3. Backlit Liquid Crystal Display (LCD) 4. Splash proof keypad 5. Lamp connector 6. Lamp holder 7. Battery compartment 4.2. KEYPAD DESCRIPTION 8. ON/OFF - press to turn the instrument ON or to activate the back light. Keep the key pressed for three seconds to turn the instrument OFF.

  • Page 11: Connectors Description

    9. GLP - press to enter/exit GLP (Good Laboratory Practice) feature. In Setup this key is used to increase the set values. In Log Recall it is used to select a new record (scroll up). 10. AVG - press to select the AVG (Signal Average Mode) on and off. In Setup it is used to decrease the set values.

  • Page 12: Display Description

    4.4. DISPLAY DESCRIPTION Battery icon is displayed when the battery voltage is getting low. Hourglass icon is displayed when the instrument performs an internal checkup. Lamp and read status indicator. Main LCD display NTU measurement units. When average or continuous mode is selected, the “NTU” tag blinks for each new displayed value.

  • Page 13: General Tips For An Accurate Measurement

    5. GENERAL TIPS FOR AN ACCURATE MEASUREMENT The instructions listed below should be carefully followed to ensure best accuracy. • Always cap the cuvettes to avoid spillage of the sample into the instrument. • Always close the lid of the instrument during measurement. •...

  • Page 14: Removing Air Bubbles

    5.5. REMOVING AIR BUBBLES Air bubbles present in the sample will cause erroneous high-turbidity readings. • To remove air bubbles fill the cuvette with wine sample and tighten the cap. Shake the cuvette gently to create pressure. Allow the cuvette to stand for a few minutes and gently invert it several times.

  • Page 15: Calibration Procedure

    6. CALIBRATION PROCEDURE It is recommended to calibrate the meter only with Hanna ready-to-use calibration standard solutions. Alternatively, formazin standards can be used. The prepared formazin solutions should be close to the default calibration points. The first point must be close to 0 NTU, the second point between 10 and 20 NTU, the third point between 50 and 150 NTU and the fourth point between 400 and 600 NTU.
  • Page 16 Note: If you’re using different calibration standards, change the displayed value by pressing UP or DOWN keys until the display shows the desired value. • Remove the <0,10 NTU standard cuvette and place the 15.0 NTU standard cuvette into the holder. •...

  • Page 17: Out Of Calibration Range Function

    (500 NTU) and “CAL P.4” is displayed. If desired, the value can be changed using the UP or DOWN keys. Note: Press CAL/RCL to exit calibration. The instrument will memorize the three-point (<0.10, 15.0 and 100 NTU) calibration data and will return to measurement mode. •...

  • Page 18: Calibration Errors

    6.3. CALIBRATION ERRORS • If the read value during calibration is too far from the set value, the instrument will show “-LO-” or “-HI-” error. • If the calculated calibration coefficients are outside specifications, the “CAL Err” message is displayed. 6.4.

  • Page 19: Turbidity Measurement

    7. TURBIDITY MEASUREMENT Note: For wine analysis it is recommended to always work with the AVG mode on. • Press ON/OFF to turn the instrument on. • When the LCD displays “----”, the instrument is ready for measurement. The current time appears on the secondary LCD, if selected in Setup menu.

  • Page 20: Avg (Signal Average Mode)

    • Press READ and “----” will blink on the display. • At the end of the measurement, the instrument directly displays turbidity in NTU. 7.1. AVG (SIGNAL AVERAGE MODE) It is recommanded to select this measurement mode when you work with unstable samples that contain suspended particles of different dimensions.

  • Page 21: Bentocheck (Protein Stability Test)

    8. BENTOCHECK (PROTEIN STABILITY TEST) • Turn the instrument ON by pressing ON/OFF. When the LCD displays “----”, the instrument is ready. Note: On the secondary LCD the current time appears, if selected in Setup menu. • Select the AVG mode by pressing the AVGkey. The AVG icon will appear on the display. •...
  • Page 22 • Press READ and “----” will blink on the display. After a few seconds the instrument displays the first reading. The meter continues updating the readings until the “Lamp and Read Status” indicator turns off. The final displayed value is the averaged reading. •...
  • Page 23 • If “T2<T1+2” the wine can be considered stable. Otherwise the wine needs to be stabilized. Note: to get more representative results of long term protein stability, HANNA Instruments® recommends to filter the wine sample first through a 0.45 micron filter disc before analysis.

  • Page 24: Determination Of Bentonite Requirement

    9. DETERMINATION OF BENTONITE REQUIREMENT 9.1. SAMPLE PREPARATION • Fill four HANNA vials with 25 mL of unfiltered wine sample. • Prepare a bentonite suspension of 2.5%. Note: Always use a bentonite suspension with the same wetting degree as the suspension that is used in production.
  • Page 25 • Close the glass vials tightly with their caps and mix thoroughly. • Wait for 15 minutes to allow suspended matter to settle. • Fold a filter disc twice as shown in the figure. • Separate one side from the other three to form a cone. Insert the folded filter disc in the funnel. •...

  • Page 26: Reading Procedure

    • Prepare fresh filters and repeat the filtration for all treated wine samples (#2, #3,#4). 9.2. READING PROCEDURE • Turn the instrument ON by pressing ON/OFF. When the LCD displays “----”, the instrument is ready. • Select the AVG mode by pressing the AVGkey. The AVG icon will appear on the display. •...
  • Page 27 • Remove cuvette #1 and open the cap. Use the 1000 µL automatic pipette to add exactly 1 mL HI83749-0 Bentocheck reagent to the cuvette. For a correct use of the automatic pipette please follow the related Instruction Sheet. • Replace the cap. Invert several times to mix and wait for 1 minute. •...

  • Page 28: Comparative Mode

    • Repeat the reading procedure for all samples (#2, #3, #4) and record all T1 and T2 values. • For each sample verify if “T2<T1+2”: if so, the wine can be considered stabilized. Compare the results. It is recommanded to choose the lowest dosage of bentonite necessary to stabilize the wine. •...

  • Page 29: Logging

    10. LOGGING HI83749 has a log space for up to 200 records. With each measurement, the date, time and tag ID is stored. In this way, each record is fully characterized and can be easily analyzed when downloading data on the PC application (HI92000). The log function is active after a valid measurement is obtained (no errors).

  • Page 30: View Logged Data

    • If the log memory is full, the “LoG FULL” message will appear for a few seconds on the LCD and the instrument will return to measurement mode without storing the new record. To store a new record, delete one or more records. 10.1.
  • Page 31 • The hexadecimal string of the tag for the sampling location ID. Note: If the logged sample value is an over range reading, the maximum value (1200) will be displayed blinking. Note: If the ID data are missing, “----” is displayed instead. •...
  • Page 32 Delete Last Record To delete the last record, scroll through the log menu until the delete last record panel is displayed. • To delete the last record, press SETUP/DEL when the “Delete last records” panel is displayed. • The instrument asks for confirmation. Press the LOG/CFM key to confirm the last record is deleted. To abort the delete function, press READ...

  • Page 33: Good Laboratory Practice (Glp)

    11. GOOD LABORATORY PRACTICE (GLP) The GLP feature allows the user to view last calibration data. Also the user calibration can be deleted. • Press GLP to enter or exit GLP data consulting. Several functions are available when in GLP menu.
  • Page 34 • Fourth calibration point (if available). • Delete calibration panel. To delete calibration: press SETUP/DEL when the instruments displays the “Delete calibration” panel. The user calibration will be deleted and the factory calibration will be restored. The instrument will enter automatically in idle mode.

  • Page 35: Setup

    12. SETUP The Setup mode allows the user to view and modify the instrument parameters. The blinking “CAL” tag appears during setup mode suggesting to press CAL for editing parameters. • To enter/exit SETUP, press SETUP/DEL. • To select the parameter to be edited, press UP or DOWN keys until the desired panel is displayed. 12.1.

  • Page 36: Activating Comparative Mode

    Note: To exit without saving the reference value, press SETUP/DEL and the meter will return to the measurement panel. • To activate the Comparative mode, press the AVG key and set ON the Comparative mode. • Press SETUP/DEL to return to Measurement mode. The display will show the reference value. •...

  • Page 37: Set Epa Compliance Mode

    • Press the UP or DOWN keys to set the Comparative mode on or off. • Press LOG/CFM to save the setting. The new selected option will be displayed on the LCD. Alternatively, press CAL/RCL to exit without saving the new settings. Note: when Comparative mode is set on, in the Measurement mode, the secondary display will show the reference value 12.3.

  • Page 38: Set Beeper

    Press LOG/CFM to save the setting. The new selected option of the parameter will be displayed on the LCD. Alternatively, press CAL to exit without saving the new settings. 12.4. SET BEEPER HI83749 has a built-in beeper that signals the read tag, the pressed key and the error conditions. The beeper can be selected to be on or off.

  • Page 39: Set The Date

    • Press the UP or DOWN keys to set LCD / hide for time. • Press LOG/CFM to save the change. The new selected option will be displayed on the LCD. Alternatively, press CAL/RCL to exit without saving the changes. 12.6.

  • Page 40: Set The Time

    • Press LOG/CFM or READto start editing the day value. The day value will start blinking. • Press the UP or DOWN keys to set the day value. Note: to edit the year again, after the day was set, press READ. •...

  • Page 41: Set Instrument Id

    • Press the UP or DOWN keys to set the minutes value. Note: To edit the hour again, after the minutes were edited, press READ. • Press LOG/CFM to save the new time. The new set time will be displayed. Alternatively, press CAL/ RCL to exit without saving the changes.

  • Page 42: Set Baud Rate

    12.9. SET BAUD RATE HI83749 has a RS232 and a USB link. When the USB connection is used, the RS232 connection becomes inactive. To successfully communicate with the PC, the same baud rate must be selected on the instrument and on the PC application. The available baud rates are 1200, 2400, 4800 and 9600. •...

  • Page 43: Tag Installation

    13. TAG INSTALLATION The tag is housed in a rugged metal that can withstand harsh environments. However, it is better to protect the tag from direct rain. Place the tag near a sampling point. Fix it securely with the provided screws, in such a way that the metallic iButton®...

  • Page 44: Lamp Replacement

    14. LAMP REPLACEMENT The instrument tungsten lamp has a life longer than 100,000 measurements. In case of lamp failure, the defective lamp can be easily replaced. When the lamp is broken, the instrument displays “no L” error message. To replace the lamp follow the next steps: •...

  • Page 45: Battery Management

    15. BATTERY MANAGEMENT For field measurements, HI83749 is powered by four AA batteries. The battery life is enough for 1500 normal measurements. When you turn the instrument on, the remaining battery life is estimated and reported in percents. To save the battery life, the instrument will turn off after 15 minutes of non use. The backlight will be turned off after 25 seconds since the last key was pressed.

  • Page 46: Batteries Replacement

    15.1. BATTERIES REPLACEMENT To replace the batteries follow the next steps: • Turn OFF the instrument by pressin ON/OFF. • Open the batteries cover by pressing the locking clip. • Take out the used batteries and insert four new 1.5 AA size batteries, paying attention to the correct polarity as indicated on the battery compartment.

  • Page 47: Error Codes

    16. ERROR CODES HI83749 has a powerful diagnostic system. Common errors are detected and reported for easy diagnostic and maintenance. ERROR DESCRIPTION ACTION Err1 – Err3; Critical errors. The instrument beeps and Contact your nearest HANNA Err6; Err7; Err8 shuts down. Customer Service Center Err4 The instrument beeps shortly twice and...

  • Page 48: Pc Interface

    HI92000 application for successful data transfer. To allow our users access to the latest version of Hanna Instruments PC compatible software, we made the products available for download at http://software.hannainst.com. Select the product code and click Download Now. After download is complete, use the setup.exe file to install the software.

  • Page 49: Accessories

    18. ACCESSORIES HI83749-20 Bentocheck (100 mL) HI93703-58 Silicone oil (15 mL) HI83749-11 Calibration cuvettes kit HI731331 Glass cuvettes (4 pcs.) HI731335N Caps for cuvettes (4 pcs.) HI93703-50 Cuvette cleaning solution (230 mL) HI731318 Tissue for wiping cuvettes (4 pcs.) HI740220 25 mL glass vial with cap (2 pcs.) HI731341 Automatic pipette 1000 µL...

  • Page 50: Certification

    If the instrument is to be returned to Hanna Instruments, first obtain a Returned Goods Authorization number from the Technical Service Department and then send it with shipping costs prepaid. When shipping any instrument, make sure it is properly packed for complete protection.
  • Page 51 Hanna Instruments reserves the right to modify the design, construction or appearance of its products without advance notice.
  • Page 52 World Headquarters Hanna Instruments Inc. Highland Industrial Park 584 Park East Drive Woonsocket, RI 02895 USA www.hannainst.com MAN83749 Printed in ROMANIA...

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