Grow master basic for nutrient analyses (51 pages)
Summary of Contents for Hanna Instruments HI83326
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HI83326 Multiparameter Photometer for Pool & Spa...
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If you need additional technical information, do not hesitate to e‑mail us at tech@hannainst.com or view our worldwide contact list at www.hannainst.com. All rights are reserved. Reproduction in whole or in part is prohibited without the written consent of the copyright owner, Hanna Instruments Inc., Woonsocket, Rhode Island, 02895, USA.
1. PRELIMINARY EXAMINATION Remove the instrument and accessories from the packaging and examine it carefully. For further assistance, please contact your local Hanna Instruments Office or email us at tech@hannainst.com. Each HI83326 is delivered in a rugged carrying case and is supplied with: •...
4. ABBREVIATIONS Absorbance Good Laboratory Practice NIST National Institute of Standards and Technology US Environmental Protection Agency grams per liter (ppt) µg/L micrograms per liter (ppb) mg/L milligrams per liter (ppm) milliliter High Range Low Range...
With the CAL Check™ feature users are able to validate the performance of the instrument and apply a user calibration (if necessary). Hanna Instruments CAL Check™ cuvettes are made with NIST traceable standards. Probe mode uses a digital pH probe with a one or two-point calibration.
5.3. FUNCTIONAL DESCRIPTION 1. Splash-proof keypad 2. Liquid Crystal Display (LCD) 3. Indexing mark 4. Protective port covers 5. Light-blocking cover panel 6. Cuvette holder 7. ON/OFF power button 8. 3.5-mm TRRS (jack) input for digital electrodes 9. Standard USB host connector for data transfer to a USB flash drive 10.
Keypad Description The keypad contains 12 direct keys and 3 functional keys with the following functions: Press the functional key to perform the function displayed above it on the LCD. Press to access the list of photometer methods. Press to move up in a menu or a help screen, to increment a set value or to access second level functions.
5.5. OPTICAL SYSTEM Instrument Block Diagram The internal reference system (reference detector) of the HI83326 photometer compensates for any drifts due to power fluctuations or ambient temperature changes, providing a stable source of light for your blank (zero) measurement and sample measurement.
Zero measurement). If a photometer measurement is on the screen, an auto-log is created before shutdown. 6.2. MODE SELECTION HI83326 has two operational modes: Photometer and Probe. Photometer mode enables on-demand measurement of a cuvette using the integrated optical system.
6.3. GENERAL SETUP Press the SETUP key to enter in Setup menu, highlight desired option using the keys and press Select. CAL Check (Photometer Mode Only) Press Select to enter the CAL Check screen. The date, time and values for the last CAL Check are displayed on the screen.
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Date & Time Press Modify to change the date and time. Press the functional keys or the keys to highlight the value to be modified (year, month, day, hour, minute or second). Use the keys to change the value.
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Language Option: Português, Deutsch, English, Español, Français, Italiano, Dutch Press Modify to change the language. Use the keys to select the desired language. Press Select to change the language. Beeper Option: Enable or Disable When enabled, a short beep is heard every time a key is pressed. A long beep alert sounds when the pressed key is not active or an error is detected.
ESC key to return to the Setup menu. 6.4. CONTEXTUAL HELP HI83326 offers an interactive contextual help mode that assists the user at any time. To access the help screen press the HELP key. The instrument will display additional information related to the current screen.
7. LOGGING DATA & DATA MANAGEMENT The instrument features a data log function to help you keep track of all your analysis. The data log can hold 1000 individual measurements. Storing, viewing and deleting the data is possible using the LOG and RECALL keys.
Explorer or Mac Finder) to move the file from the meter to the PC. Log data is exported as a single file (HI83326.csv) containing all logged photometer and probe data. The CSV file may be opened with a text editor or spreadsheet application.
8. PHOTOMETER MODE 8.1. METHOD SELECTION In order to select the desired method press the METHOD key and a screen with the available methods will appear. Press the keys to highlight the desired method. Press Select. After the desired method is selected, follow the procedure described in the related section. Before performing a method, read all the instructions carefully.
Proper Use of Dropper Bottle 1. Tap the dropper on the table several times. 2. Remove the cap and wipe the outside of the tip with a cloth. 3. Keep the dropper bottle in a vertical position while dosing the reagent. Proper Use of Powder Packet 1.
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(b) Shaking the cuvette, moving the cuvette up and down. The movement may be gentle or vigorous. This mixing technique is indicated with “shake gently” or “shake vigorously”, and one of the following icons: shake gently shake vigorously (c) Swirl the cuvette gently to mix the solution. This mixing technique is indicated with one of the following icons: In order to avoid reagent leaking and to obtain more accurate measurements, close the cuvette first with the supplied High-Density Polyethylene (HDPE)
Discard the sample immediately after the reading is taken, or the glass might become permanently stained. All the reaction times reported in this manual are at 25 °C (77 °F). In general, the reaction time should be increased for temperatures lower than 20 °C (68 °F) and decreased for temperatures higher than 25 °C (77 °F).
8.6. METER VALIDATION & CAL CHECK ® Warning: Do not validate the meter with standard solutions other than the Hanna Instruments CAL Check Standards. For accurate validation results, please perform tests at room temperature, 18 to 25 ° C (64.4 to 77.0 ° F).
4. Insert a cuvette filled with deionized water, then press Zero. 5. Insert the prepared sample cuvette, then press Read. ® Warning: Never use absorbance methods for validation using Hanna Instruments CAL Check cuvettes. The factory calibration corrections for CAL Check cuvettes are applied while in CAL Check...
® HI83326 can be used to perform direct pH measurements by connecting a Hanna Instruments digital pH electrode with a 3.5 mm TRRS connector. Connect the electrode to the 3.5 mm port marked with EXT PROBE located at the rear of the meter. If the meter is in Photometer mode, set the meter to Probe mode by pressing the MODE key.
9.2. pH CALIBRATION From the probe measurement screen, press Calibrate to begin the calibration process. During pH calibration, the display will show the current pH reading, temperature reading, selected buffer type and the buffer number (“Buffer: 1” for the first buffer, “Buffer: 2” for the second buffer). Press Clear to clear the current calibration.
9.3. pH MESSAGES & WARNINGS No Probe No probe is connected or the probe is broken. Connecting The meter has detected a probe and is reading the probe configuration and calibration information. Incompatible Probe The connected probe is not compatible with this device. Incompatible Calibration The probe’s current calibration is not compatible with this meter.
Check Probe & Buffer There is a large difference between the pH measurement and the selected buffer value or the electrode slope is outside of the accepted slope limit. Clean the probe and confirm the correct buffer selection. Wrong Temperature The buffer temperature is outside of the acceptable window for the selected buffer value.
9.5. pH ELECTRODE CONDITIONING & MAINTENANCE Remove the protective cap. Do not be alarmed if salt deposits are present, this is normal. Rinse the probe with water. Shake the electrode down as you would do with a clinical thermometer to eliminate any air bubbles inside the glass bulb.
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Periodic Maintenance Inspect the electrode and the cable. The cable used for connection to the instrument must be intact and there must be no points of broken insulation on the cable, connectors must be perfectly clean and dry. If there are any scratches or cracks on the electrode stem or bulb, replace the electrode. For refillable electrodes, refill the reference chamber with fresh electrolyte (HI7082 HI8082...
10. METHOD PROCEDURES 10.1. ALKALINITY SPECIFICATIONS Range 0 to 500 mg/L (as CaCO Resolution 1 mg/L Accuracy ±5 mg/L ±5% of reading at 25 °C Light Source LED with narrow band interference filter @ 610 nm Method Colorimetric Method REQUIRED REAGENTS Code Description Quantity...
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• Remove the cuvette. • Add 1mL of HI775S Alkalinity Reagent to the sample using a 1 mL syringe. 1 mL • Replace the plastic stopper and the cap. Invert 5 times. • Insert the cuvette into the holder and close the lid. •...
10.2. BROMINE SPECIFICATIONS Range 0.00 to 8.00 mg/L (as Br Resolution 0.01 mg/L Accuracy ±0.08 mg/L ±3% of reading at 25 °C Light Source LED with narrow band interference filter @ 525 nm Method Adaptation of Standard Methods for the Examination of Water and Wastewater, 18 Edition, DPD Method REQUIRED REAGENTS...
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• Remove the cuvette. • Add one packet of HI93716-0 Bromine Reagent. Replace the plastic stopper and the cap. Shake gently for about 20 seconds to dissolve most of the reagent. • Insert the cuvette into the holder and close the lid. •...
10.3. CHLORINE DIOXIDE SPECIFICATIONS Range 0.00 to 2.00 mg/L (as ClO Resolution 0.01 mg/L Accuracy ±0.10 mg/L ±5% of reading at 25 °C Light Source LED with narrow band interference filter @ 575 nm Method Adaptation of the Chlorophenol Red Method REQUIRED REAGENT Code Description...
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• Add 0.5 mL of HI93738A-0 Chlorine Dioxide Reagent A to each cylinder (#1 & #2), using a 1 mL syringe, cap them and invert several times to mix. #1 & #2 0.5 mL 0.5 mL • Add one packet of HI93738B-0 Chlorine Dioxide Reagent B to one of the two cylinders (#1), cap and invert it several times until it is totally dissolved.
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• Add 0.5 mL of HI93738D-0 Chlorine Dioxide Reagent to each cylinder (#1 & #2), using a 1 mL syringe, cap them and invert several times to mix. Cylinder #2 is the reacted sample. #1 & #2 0.5 mL 0.5 mL •...
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• Press Read to start the reading. The instrument displays the results in mg/L of chlorine dioxide (ClO INTERFERENCES Interference may be caused by: • Strong oxidants...
10.4. CHLORINE DIOXIDE, RAPID METHOD SPECIFICATIONS Range 0.00 to 2.00 mg/L (as ClO Resolution 0.01 mg/L Accuracy ±0.10 mg/L ±5% of reading at 25 °C Light Source LED with narrow band interference filter @ 525 nm Method Adaptation of Standard Methods for the Examination of Water and Wastewater, Edition, 4500 ClO REQUIRED REAGENT Code...
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MEASUREMENT PROCEDURE • Select the Chlorine Dioxide (Rapid) method using the procedure described in the METHOD SELECTION section. 10 mL • Fill the cuvette with 10 mL of unreacted sample (up to the mark). • Add 5 drops of HI96779A-0 Chlorine Dioxide Reagent A.
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• Remove the cuvette. • Add one packet of HI96779B-0 Chlorine Dioxide Reagent B. • Replace the plastic stopper and the cap. Shake gently for 20 seconds. • Insert the cuvette into the holder and close the lid. • Press Timer and the display will show the countdown prior to the measurement or wait 1 minute and press Read.
10.5. CHLORINE, FREE SPECIFICATIONS Range 0.00 to 5.00 mg/L (as Cl Resolution 0.01 mg/L Accuracy ±0.03 mg/L ±3% of reading at 25 °C Light Source LED with narrow band interference filter @ 525 nm Method Adaptation of the EPA DPD Method 330.5 REQUIRED REAGENTS POWDER Code...
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• Press Zero. The display will show “-0.0-” when the meter is zeroed and ready for measurement. • Remove the cuvette. POWDER REAGENT PROCEDURE • Add the content of one packet of HI93701-0 Free Chlorine Reagent. Replace the plastic stopper and the cap.
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LIQUID REAGENT PROCEDURE • To an empty cuvette add 3 drops of HI93701A-F Free Chlorine Reagent A and 3 drops of HI93701B-F Free Chlorine Reagent B. ×3 ×3 • Replace the plastic stopper and the cap. Swirl gently to mix. •...
10.6. CHLORINE, TOTAL SPECIFICATIONS Range 0.00 to 5.00 mg/L (as Cl Resolution 0.01 mg/L Accuracy ±0.03 mg/L ±3% of reading at 25 °C Light Source LED with narrow band interference filter @ 525 nm Method Adaptation of the EPA DPD Method 330.5 REQUIRED REAGENTS POWDER Code...
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• Press Zero. The display will show “-0.0-” when the meter is zeroed and ready for measurement. • Remove the cuvette. POWDER REAGENT PROCEDURE • Add 1 packet of HI93711-0 Total Chlorine Reagent. Replace the plastic stopper and the cap. Shake gently for 20 seconds.
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LIQUID REAGENT PROCEDURE • To an empty cuvette add 3 drops of HI93701A-T Total Chlorine Reagent A, 3 drops of HI93701B-T ×3 ×3 ×1 Total Chlorine Reagent B and 1 drop of HI93701C-T Total Chlorine Reagent C. • Replace the plastic stopper and the cap. Swirl gently to mix.
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INTERFERENCES Interference may be caused by: • Bromine, Iodine, Oxidized forms of Chromium and Manganese, Ozone • Hardness greater than 500 mg/L CaCO , to remove the interference shake the sample for approximately 2 minutes after adding the powder reagent •...
10.7. COPPER HIGH RANGE SPECIFICATIONS Range 0.00 to 5.00 mg/L (as Cu Resolution 0.01 mg/L Accuracy ±0.02 mg/L ±4% of reading at 25 °C Light Source LED with narrow band interference filter @ 575 nm Method Adaptation of the EPA Method REQUIRED REAGENTS Code Description...
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• Remove the cuvette. • Add one packet of HI93702-0 Copper High Range Reagent. Replace the plastic stopper and the cap. Shake gently for about 15 seconds. • Insert the cuvette into the holder and close the lid. • Press Timer and the display will show the countdown prior to the measurement or wait 45 seconds and press Read.
10.8. CYANURIC ACID SPECIFICATIONS Range 0 to 80 mg/L (as CYA) Resolution 1 mg/L Accuracy ±1 mg/L ±15% of reading at 25 °C Light Source LED with narrow band interference filter @ 525 nm Method Adaptation of the Turbidimetric Method REQUIRED REAGENTS Code Description...
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• Fill a beaker with 25 mL sample. • Add one packet of HI93722-0 Cyanuric Acid Reagent and mix to dissolve. • Fill a second cuvette with 10 mL of the reacted sample (up to the mark). Replace the plastic stopper and the cap. 10 mL •...
10.9. HARDNESS, CALCIUM SPECIFICATIONS Range 0.00 to 2.70 mg/L (as CaCO Resolution 0.01 mg/L Accuracy ±0.11 mg/L ±5% of reading at 25 °C Light Source LED with narrow band interference filter @ 525 nm Method Adaptation of Standard Methods for the Examination of Water and Wastewater, 18 Edition, Calmagite Method REQUIRED REAGENTS...
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×1 • Add 1 drop of HI93720C-0 Calcium Hardness Reagent C to one cuvette (#1). • Replace the plastic stopper and the cap. Invert the cuvette several times to mix. This is the blank. • Insert the blank (#1) into the holder and close the lid. •...
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• Press the or key to access the second level functions. • Press Chem Frm to convert the results to French degrees (°f), German degrees (°dH) and English degrees (°E). • Press the or key to return to the measurement screen. Note: This test will detect any calcium contamination in the beaker, measuring syringes or sample cells.
10.10. IRON HIGH RANGE SPECIFICATIONS Range 0.00 to 5.00 mg/L (as Fe) Resolution 0.01 mg/L Accuracy ±0.04 mg/L ±2% of reading at 25 °C Light Source LED with narrow band interference filter @ 525 nm Method Adaptation of Standard Methods for the Examination of Water and Wastewater, 23 Edition, 3500-Fe B, Phenanthroline Method REQUIRED REAGENTS...
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• Remove the cuvette and add the content of one packet of HI93721-0 Iron High Range Reagent. Replace the plastic stopper and the cap. Shake until powder is completely dissolved. • Insert the cuvette into the holder and close the lid. •...
10.11. NITRATE SPECIFICATIONS Range 0.0 to 30.0 mg/L (as NO - N) Resolution 0.1 mg/L Accuracy ±0.5 mg/L ±10% of reading at 25 °C Light Source LED with narrow band interference filter @ 525 nm Method Adaptation of the Cadmium Reduction Method REQUIRED REAGENTS Code Description...
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• Remove the cuvette and add one packet of HI93728-0 Nitrate Reagent. • Replace the plastic stopper and the cap. Shake vigorously up and down for exactly 10 seconds. Continue to mix by inverting the cuvette gently for 50 seconds, while taking care not to induce air bubbles.
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• Press Chem Frm to convert the result to mg/L of nitrate (NO ¯). • Press the or key to return to the measurement screen. INTERFERENCES Interference may be caused by: • Ammonia and amines, as urea and primary aliphatic amines •...
10.12. OZONE SPECIFICATIONS Range 0.00 to 2.00 mg/L (as O Resolution 0.01 mg/L Accuracy ±0.02 mg/L ±3% of reading at 25 °C Light Source LED with narrow band interference filter @ 525 nm Method Colorimetric DPD Method REQUIRED REAGENTS Code Description Quantity HI93757-0...
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• Remove the cuvette. • Add one packet of HI93757-0 Ozone Reagent. Replace the plastic stopper and the cap. Shake gently for 20 seconds. • Insert the cuvette into the holder and close the lid. • Press Timer and the display will show the countdown prior to the measurement or wait 2 minutes and press Read.
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• Press Zero. The display will show “-0.0-” the meter is zeroed and ready for measure. • Remove the cuvette. • Add one packet of the HI93703-52-0 Glycine Powder. Replace the plastic stopper and the cap. Shake gently until the powder is completely dissolved. •...
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• To determine the mg/L ozone (O ) concentration in sample containing chlorine, subtract value B (additional measurement procedure) from value A (standard measurement procedure). INTERFERENCES Interference may be caused by: • Bromine, Chlorine Dioxide, Iodine • Hardness greater than 500 mg/L CaCO , shake the sample for approximately 2 minutes after adding the powder reagent •...
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10.13. pH SPECIFICATIONS Range 6.5 to 8.5 pH Resolution 0.1 pH Accuracy ±0.1 pH at 25 °C Light Source LED with narrow band interference filter @ 525 nm Method Adaptation of the Phenol Red Method REQUIRED REAGENTS Code Description Quantity HI93710-0 pH Reagent 5 drops...
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• Remove the cuvette and add 5 drops of HI93710-0 ×5 Reagent Indicator. Replace the plastic stopper and the cap and mix the solution. • Insert the cuvette into the holder and close the lid. • Press Read to start the reading. The instrument displays the result in pH.
10.14. PHOSPHATE LOW RANGE SPECIFICATIONS Range 0.00 to 2.50 mg/L (as PO Resolution 0.01 mg/L Accuracy ±0.04 mg/L ±4% of reading at 25 °C Light Source LED with narrow band interference filter @ 610 nm Method Adaptation of the Ascorbic Acid Method REQUIRED REAGENTS Code Description...
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• Insert the cuvette into the holder and close the lid. • Press Timer and the display will show the countdown prior to the measurement or wait 3 minutes and press Read. When the timer ends the meter will perform the reading. The instrument displays concentration in mg/L of phosphate (PO ¯).
There is an excess amount of ambient light reaching the detector. Make sure the lid is closed before performing any measurements. If the issue persists, please contact Hanna Instruments technical support. The sample and the zero cuvettes are inverted. Swap the cuvettes and repeat the measurement.
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The measured value cannot be calculated. Please check sample preparation and measurement procedure. Stored results of the CAL Check measurements have been lost. Please redo the CAL Check measurements to ensure accurate results. User settings have been lost. Please reset the values. If the issue persists, please contact Hanna Instruments technical support.
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Real time clock is not accurate. Some features are no longer available. Restart the meter. If the issue persists, please contact Hanna Instruments technical support. The device serial number can not be identified. Some features are no longer available. Restart the meter. If the issue persists, please contact Hanna Instruments technical support.
ELECTRODE CLEANING SOLUTIONS Code Description HI70000P Electrode rinse sachet, 20 mL (25 pcs.) HI7061L General cleaning solution, 500 mL HI7073L Protein cleaning solution, 500 mL HI7074L Inorganic cleaning solution, 500 mL HI7077L Oil & fat cleaning solution, 500 mL HI8061L General cleaning solution in FDA approved bottle, 500 mL HI8073L Protein cleaning solution in FDA approved bottle, 500 mL...
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USB power adapter, European plug HI75110/220U USB power adapter, USA plug HI76404A electrode holder HI83326-11 CAL Check cuvette kit for HI83326 Sample preparation kit consisting of activated carbon for 50 tests, demineralizer bottle for 10 L of water, 100 mL graduated beaker with cap,...
Damage due to accidents, misuse, tampering or lack of prescribed maintenance is not covered. If service is required, contact your local Hanna Instruments Office. If under warranty, report the model number, date of purchase, serial number (engraved on the bottom of the meter) and the nature of the problem.
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Hanna Instruments reserves the right to modify the design, construction or appearance of its products without advance notice.
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World Headquarters Hanna Instruments Inc. Highland Industrial Park 584 Park East Drive Woonsocket, RI 02895 USA www.hannainst.com MAN83326 Printed in ROMANIA...