Assembling The Upper Buffer Chamber - BIO RAD Mini-PROTEAN II Assembly Manual

Mini-PROTEAN II Cell Assembly Guide

Assembling the Upper Buffer Chamber

1. To insure a leakproof seal, make sure that the gray, U-shaped,
inner core gaskets are clean and that they are installed with
the notched side exposed for contact with the gel sandwich.
2. Lay the electrode assembly on a flat surface. With the glass
plates facing the inner core (and the clamp screws facing up),
place the top of the clamp assembly underneath the locator slots
on the electrode assembly and rest the bottom of the clamp
assembly on the base plate. Carefully slide the clamp assembly
forward, until the inner glass plate of the gel sandwich butts
against the notch in the U-shaped gasket.
N o t e : If the clamp is introduced at too steep an angle, the
inner glass plate will pinch the notched gasket and leaking will
result. Lubricate the raised portions of the gasket with a drop of
running buffer or water to help the glass plate sandwich slide
in properly. It is extremely important that the short glass plate is
firmly seated against the notch in the gasket, or buffer leaks will
occur. Visually inspect the glass plate/notch interface to insure
correct assembly.
3. When the inner plate is in position, push down on the base of the
clamp assembly until the latch engages each side.
4. Turn over the electrode assembly and attach another clamp
assembly to the other side in the same manner.
5. If only one gel is to be run, a buffer dam may be made by
inserting a short (inner) glass plate and a long (outer) glass plate
w i t h o u t spacers into the clamp assembly. Align the plates using
the alignment position of the casting stand, tighten the clamp
screws, and snap onto the electrode assembly as usual.
1
2a
2b
3