Scanning An Image - Zeiss LSM 5 MP Quick Manual

Scanning an image

Setting the parameters for scanning
" Select the Acquisition Mode tool from the Left Tool Area (Fig. 18).
" Select the Frame Size as predefined number of pixels or enter your own values (e.g. 300 x 600) in the
Acquisition Mode tool. Click on the Optimal button for calculation of appropriate number of pixels
depending on objective N.A. and %.
The number of pixels influences the image resolution!
Fig. 18 Acquisition Mode tool
Note: When using an Axioskop 2 FS
Acquisition Mode tool. This ensures correct calibration, calculation of pinhole and Z stack
optimization, etc. The Axioskop 2 FS
Adjusting scan speed
" Use the Scan Speed slider in the Acquisition Mode tool (Fig. 18) to adjust the scan speed.
A higher speed with averaging results in the best signal-to-noise ratio. Scan speed 8 usually produces
good results. Use speed 6 or 7 for superior images.
Choosing the dynamic range
" Select the dynamic range 8 or 12 Bit (per pixel) in the Bit Depth pull-down in the Acquisition Mode
tool (Fig. 18).
8 Bit will give 256 gray levels; 12 Bit will give 4096 gray levels. Publication quality images should be
acquired using 12 Bit data depth. 12 Bit is also recommended when doing quantitative measurements or
when imaging low fluorescence intensities.
08/2007
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, make sure you set the correct objective manually in the
MOT
does not automatically detect the objective lens.
MOT
15