Scanning An Image; Setting The Parameters For Scanning; Adjusting Scan Speed; Choosing The Dynamic Range - Zeiss LSM 700 Quick Manual

Scanning an image

Setting the parameters for scanning

Select the Acquisition Mode tool from the Left Tool Area (Fig. 20).
Select the Frame Size as predefined number of pixels or enter your own values (e.g. 300 x 600) in the
Acquisition Mode tool. Click on the Highest button for calculation of appropriate number of pixels
depending on objective N.A. and .
The number of pixels influences the image resolution!
Fig. 20 Acquisition Mode tool

Adjusting scan speed

Use the Scan Speed slider in the Acquisition Mode tool (Fig. 20) to adjust the scan speed.
A higher speed with averaging results in the best signal-to-noise ratio. Scan speed 8 usually produces
good results. Use speed 6 or 7 for superior images.

Choosing the dynamic range

Select the dynamic range 8 or 12 or 16 Bit (per pixel) in the Bit Depth pull-down in the Acquisition
Mode tool (Fig. 20).
8 Bit will give 256 gray levels; 12 Bit will give 4096 gray levels, 16 Bit will give 65536 gray levels.
Publication quality images should be acquired using 12 Bit data depth. 12 or 16 Bit is also recommended
when doing quantitative measurements or when imaging low fluorescence intensities.
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