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pyroscience FireStingGO2 Manual

Pocket oxygen meter
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Pocket Oxygen Meter
FireStingGO
2
M
ANUAL
www.pyroscience.com
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  • Page 1 Pocket Oxygen Meter FireStingGO ANUAL www.pyroscience.com...
  • Page 2 Document Version 1.21 Refers to FireStingGO Manager software version >1.0 FireStingGO is manufactured by PyroScience GmbH Hubertusstr. 35 52064 Aachen Germany Phone +49 (0)241 518322-10 +49 (0)241 518322-99 Email info@pyroscience.com Internet www.pyroscience.com Registered: Aachen HRB 17329, Germany...

  • Page 3: Table Of Contents

    ABLE OF ONTENT INTRODUCTION ..............6 SAFETY GUIDELINES .............. 8 OVERVIEW FIRESTINGGO2 ............ 11 ..........11 ORTS AND NTEGRATED ENSORS 3.1.1 Micro USB Port ..............12 3.1.2 Oxygen Port ..............12 3.1.3 Temperature Port .............. 12 3.1.4 Internal Air Sensor ............. 12 3.1.5...
  • Page 4 ..............31 TART OGGING 7.1.1 Manual Logging..............31 7.1.2 Continuous Logging ............32 ..............33 OGGING STANDBY MODE ..............35 PC MODE ................35 FIRESTINGGO2 MANAGER ............. 36 10.1 ............36 OFTWARE NSTALLATION 10.2 ........36 PERATION WITHOUT ONNECTED EVICE 10.3 ........
  • Page 5 10.3.7 Options ................. 50 10.4 ........52 NSPECTOR AND ANAGEMENT 10.4.1 File Management............52 10.4.2 Log Inspector Window ........... 53 WARNINGS ................56 11.1 ............. 56 XYGEN ENSOR ARNINGS 11.2 ..........57 EMPERATURE ENSOR ARNING CALIBRATION STANDARDS ........... 59 12.1 ..........

  • Page 6: Introduction

    1 Introduction FireStingGO2 is a hand-held fiber-optic oxygen meter based on the established FireSting technology featuring:  broad oxygen sensor portfolio (micro- and minisensors, robust probes, sensor spots, flow-through cells, respiration vials)  sensors for the full and the trace oxygen range ...
  • Page 7 The new pocket meter FireStingGO2 completes the portfolio of different fiber-optic oxygen meters from PyroScience, comprising PC-operated 1-, 2- or 4-channel FireStingO2 meters with a broad oxygen sensor portfolio and the 1-channel Piccolo2 meter for advanced applications of contactless sensors, e.g. in microfluidics.

  • Page 8: Safety Guidelines

    2 Safety Guidelines FireStingGO is a laboratory instrument to be used with fiber- optic oxygen sensors (optodes) from PyroScience. In order to guarantee an optimal performance of the FireStingGO , please follow these operation instructions and safety guidelines. If any problems or damage evolve, please turn the instrument off and disconnect it immediately.
  • Page 9 When used in the field, the environmental conditions (like high humidity, dust, exposure to direct solar radiation) may cause damage or interference of the FireStingGO , which is on the user's authority. Before using the oxygen meter FireStingGO2 and its sensors, read carefully the available instructions and user manuals.
  • Page 10 In case of problems or damage, turn the instrument off, disconnect and mark it to prevent any further use! Consult PyroScience for advice! There are no serviceable parts inside the device. Opening the housing will void the warranty! FireStingGO2 is not water-proof, is sensitive to corrosive conditions changes temperature causing condensation.

  • Page 11: Overview Firestinggo2

    3 Overview FireStingGO This chapter provides an overview about the principle components and general operation procedures of the pocket oxygen meter FireStingGO 3.1 Ports and Integrated Sensors FireStingGO has three ports and one integrated sensor:  micro USB port for charging and communication ...

  • Page 12: Micro Usb Port

    FireStingGO device. 3.1.2 Oxygen Port Fiber-optic oxygen sensors from PyroScience with ST-connectors are connected to the oxygen port. Please refer to the website of PyroScience concerning the broad range of available oxygen sensor types. Remove the protective caps from the oxygen port as well as from the ST-connector of the oxygen sensor.

  • Page 13: Power Button And Recharging The Battery

    USB port (with the included USB cable). Typically the battery is fully recharged within ca. 2 hours. 3.2 Stand Clamp FireStingGO2 can be optionally mounted on common lab stands with the included stand clamp. For this, remove the red silicone shell from the device and mount the stand clamp as shown in the image.

  • Page 14: User Interface

    3.3 User Interface The user interface of the FireStingGO consists of the display and four buttons. The high-contrast display features ultra-low power consumption. Therefore it is permanently activated without significantly reducing the run-time of the rechargeable battery. The four buttons allow for an intuitive stand-alone operation of the FireStingGO OK Button: If pressed for 1 second, the device is switched on or off.

  • Page 15: Operation Modes

    3.4 Operation Modes The user interface of the FireStingGO meter comprises five different operation modes: Live Mode, Menu Mode, Logging Mode, Standby Mode, and PC Mode. 3.4.1 Live Mode After switching on the FireStingGO , the device starts in the Live Mode. Here the device performs a measurement every 2 seconds and shows the results in altogether four sub-screens.

  • Page 16: Standby Mode

    revert to the Live Mode. For more details on the logging mode refer to chapter 7. 3.4.4 Standby Mode The device automatically enters the Standby Mode, if the user did not press any button within 5 minutes in the Live Mode. Here the measurements are stopped to prolong the lifetime of the connected oxygen sensor.
  • Page 17 FireStingGO Manager software has been started on an external computer (connected via the USB port). In the Live, PC or Logging Mode, an additional bar graph and a numerical value show the so-called "signal intensity". It provides important information on the actual condition and remaining lifetime of the connected oxygen sensor.

  • Page 18: Quick Start

    FireStingGO (chapter 3.1). Start FireStingGO2 meter by pressing the OK button for about 1 sec. Check the battery charge level of the device in the status line (chapter 3.5) and recharge the battery if necessary (chapter 3.1.5).

  • Page 19: Live Measurements

    Download and install the FireStingGO Manager software from PyroScience website to a Windows PC. Connect the FireStingGO device with the included USB cable, and start the software. Go to the File tab, select a log file and click on Download &...

  • Page 20: Live Mode

    5 Live Mode After power-up or after activation from standby the FireStingGO is in the Live Mode. Measurements are performed every 2 seconds. The different sub-screens accessed with the UP and DOWN buttons. Note, that the data are NOT logged in the Live Mode. 5.1 Top Screen Here, the current reading of the connected oxygen sensor and the...

  • Page 21: Menu Mode

    6 Menu Mode The menu is entered by pressing the OK button in the Live Mode, opening the main menu. 6.1 Main Menu The main menu has four entries:  Settings: opens the Settings Menu (chapter 6.2)  Calibration: opens the Calibration Menu (chapter 6.3) ...

  • Page 22: Medium

    6.2.2 Medium Adjust here the medium Gas or Water of your sample. 6.2.3 Oxygen Units Select here your preferred oxygen unit. Note, that the available oxygen units depend on the chosen medium. 6.2.4 Temperature Here the temperature of the sample has to be adjusted.

  • Page 23: Salinity

    If your sample is kept at a constant temperature, then the automatic temperature compensation can be deactivated by selecting Fixed. Now you must enter the fixed temperature of your sample used for the temperature compensation of the oxygen measurement. 6.2.5 Salinity The salinity of the sample only has to be adjusted for measurements in saline samples (e.g.

  • Page 24: Sensor Code

    the 0% calibration is taken from the factory calibration encoded in the Sensor Code. It is recommended to perform the calibration at conditions close to the environmental conditions during the measurements, especially regarding the temperature. 6.3.1 Sensor Code For security the Calibration menu repeats the Sensor Code entry, which is in fact identical to the Sensor Code entry given in the Settings menu (chapter 6.2.1).
  • Page 25 Now the Calibration Medium of the air calibration standard needs to be defined as Ambient Air or Water, depending on the environmental sample under investigation. It is recommended to perform the calibration in the same medium as the one in the sample. 6.3.2.1 Calibration in Ambient Air Calibration in ambient air is recommended, if the measurements on the sample are done in the gas phase.
  • Page 26 Now Wait for Steady State until the sensor readings are stable. Then press SET AIR and the current oxygen sensor reading is taken for the air calibration. After the completion of the air calibration, the system returns to the Calibration menu, showing now the date of the air calibration.

  • Page 27: 0% Calibration

    Then press SET AIR and the current oxygen sensor reading are taken for the air calibration. After the completion of the air calibration, the system returns to the Calibration menu, showing now the date of the air calibration. Note: SET AIR is only active if the oxygen sensor readings are within the expected range for the connected sensor type.

  • Page 28: Options Menu

    sensor readings are stable. Note: If using retractable needle-type sensors (e.g. OXR50, OXR230, OXR430), it is important that the sensor tip is extended! Then press SET 0% and the actual oxygen sensor reading is taken for the 0% calibration. After the completion of the 0% calibration, the system returns to the Calibration Menu, showing now the date of the 0% calibration.

  • Page 29: Temperature Offset

    6.4.2 Temperature Offset It is recommended to check the reading of the PT100 temperature sensor periodically in a water bath of known temperature. This is especially important if a concentration unit (like µmol/L or mg/L) was selected for the oxygen readings (see chapter 6.2.3).

  • Page 30: Advanced Menu

    Selecting Delete All will irrevocably delete all logged data on the internal device memory! 6.5 Advanced Menu The sub-menu Advanced includes advanced features only for advanced users with special instructions from PyroScience. It is not recommended to change these advanced parameters without the advice of PyroScience!

  • Page 31: Data Logging

    7 Data Logging Before starting data logging on the internal device memory, ensure that the following steps have been performed:  Adjustment of Time and Date (see chapter 6.4.1)  Adjustment of the sensor Settings (see chapter 6.2)  Calibration of the connected oxygen sensor (see chapter 6.3) 7.1 Start Logging In order to start the logging, select Start Logging in the main menu (see chapter 6.1) and confirm...

  • Page 32: Continuous Logging

    7.1.2 Continuous Logging Select the logging mode Continuous and press the OK button. In this mode data points are periodically logged with adjustable sample intervals and total logging duration. Select the Logging Interval (1 s, 2 s, 5 s, 10 s, 30 s, 1 min, 2 min, 5 min, 10 min, 30 min, 1 hour, 2 hours or 4 hours) and press NEXT.

  • Page 33: Logging Mode

    7.2 Logging Mode Each time a new logging is started, a new log file (LOGxxx) is generated and numbered sequentially upwards (e.g. LOG001, LOG002, LOG003 etc.). In this log file, each recorded data point is numbered sequentially upwards (#xxx). The log file and the last logged data point number are underneath the status line in the top screen of the Logging Mode.
  • Page 34 In sub-screen 2 the last 100 data points of the compensation temperature are displayed in a graph. The last logged compensation temperature is shown additionally as numerical value. Sub-screen 3 shows information concerning the Environment, including the readings of the temperature sensor (if connected), as well as the air pressure (mbar) and relative humidity (%RH) measured by the internal air sensor.

  • Page 35: Standby Mode

    8 Standby Mode If no buttons are pressed in the Live Mode within 5 min, the device enters the Standby Mode. Here the device clock is shown in the display. Standby Mode measurements are stopped in order to safe lifetime of the connected oxygen sensor. To return to the Live Mode, any button can be pressed, opening the top screen of the Live Mode with the actual readings of the connected sensors.

  • Page 36: Firestinggo2 Manager

    First connect the FireStingGO meter to the PC with the included micro-USB cable, now start the software FireStingGO Manager. The FireStingGO2 will switch into the PC mode, indicated by the "PC" symbol in the upper right corner of the LCD display.

  • Page 37: Live Graph Window

    The buttons in the bottom line open the Log Inspector and the Live Graph window (more details below). FireStingGO2 Manager allows full control on the device, offering most features similar to the LCD user interface of the FireStingGO2. Before starting live measurements or data logging, following steps need to be done: ...
  • Page 38 The Live Display shows numerical live readings of all sensors comprising oxygen, compensation temperature, temperature (if a temperature sensor is connected), and the readings of the internal air sensor (air pressure, relative humidity). For more details refer to chapter 5. Above the oxygen value, an additional bar graph and a numerical value show the so-...
  • Page 39 If data logging is active, the Log Name, the Start time of the logging and the Comment entered before the start of the logging, are displayed above the graph. As default shown in the Live Graph are the current readings for oxygen and for the compensation temperature (Comp.

  • Page 40: Settings

    Autoscroll button allows inspection of older data with the scroll bar. Smart Autoscale Y will trigger an autoscale of the y axes with "smart" ranges for better readability. Autoscale X & Y performs a classical autoscale of all x and y axes. Click on Adjust Scale in order to adjust all scales manually by entering the maximum, minimum, and the increment (tag spacing) for all x and y axes.
  • Page 41 position of the respective sensor code and click on OK. Changing the Sensor Code, e.g. in case of connecting a new sensor, requires a new calibration of the oxygen sensor! Then define the medium of your sample by clicking in the field Medium and selecting Gas or Water, depending on the environmental sample under investigation.

  • Page 42: Calibration

    For measurements in gas samples this value has no relevance (and is not shown). 10.3.3 Calibration The oxygen sensor is calibrated in the Calibration tab. If a sensor is not yet calibrated, a warning Not Calibrated replaces the oxygen unit in the live graph.

  • Page 43: Air Calibration

    Position the sensor tips in close proximity to the FireStingGO2 device. Ensure that the oxygen and the temperature sensor are completely dry; otherwise the relative humidity around the sensors will differ from the measured humidity inside the FireStingGO2.
  • Page 44 It is important that the sensor in the calibration standard is exposed to the same atmospheric pressure as the FireStingGO2. Note: If using retractable needle-type oxygen sensors (e.g. OXR50, OXR230, OXR430), it is important that the sensor tip is extended when the calibration value is taken.

  • Page 45: 0% Calibration

    (4) Wait for Steady State until the oxygen and temperature readings are stable by observing the Live Graph window (see 10.3.1). (5) Calibrate by clicking on Set Air. The current oxygen sensor reading is taken for the air calibration and the date of the last calibration is updated in the Calibration tab.
  • Page 46 NOTE: Ensure constant calibration conditions! If the calibration is performed with Fixed temperature, the temperature in the calibration standard must be measured and kept constant! (2) Put Sensors into Calibration Standard. Now insert the oxygen sensor into the calibration standard. If Sensor was selected for the calibration temperature (see above), then insert also the connected temperature sensor into the calibration standard.

  • Page 47: Data Logging

    Logging tab of the main window. First check the current device time (i.e. the time of the internal clock in the FireStingGO2) shown in this tab. The time and date information for the logged data are always related to...
  • Page 48 If it is incorrect, click on Set Device Time. This opens a window for adjusting the device time. Clicking on Synchronize will set it to the PC Time. Alternatively the time and date can be adjusted manually. For this, edit the time and date below "Set Manually", and click on Set.
  • Page 49 Sample. A single data point is then saved to the log file and added to the graph in the Live Graph window. FireStingGO2 performs in this logging mode additional intermediate measurements with 2 seconds interval which are displayed only in the Live Display (see 10.3.1) but NOT in the Live...

  • Page 50: Options

    The header of each log file contains this Device ID. This way each log file can be unequivocally assigned to a specific FireStingGO2 device. The following subsections describe the other feature within the Options tab.
  • Page 51 10.3.7.3 Advanced Mode The Advanced Mode comprises advanced features only for advanced users with special instructions from PyroScience. It is NOT recommended to change these advanced parameters without the advice of PyroScience!

  • Page 52: Log Inspector And File Management

    10.4 Log Inspector and File Management The actual data logging is always done on the internal device memory of the FireStingGO2. The File tab in the main window provides a list of all log files currently saved in the device memory.

  • Page 53: Log Inspector Window

    FireStingGO2 Device ID xxx within the default user document folder on the Windows PC. It is recommended to keep this default folder unchanged. During downloading, the progress of the download is indicated and can be stopped by clicking on the red stop button.
  • Page 54 Note: The default location of the download folder contains the Device ID (see chapter 10.3.7), which ensures that the downloaded files can be unequivocally assigned to a specific FireStingGO2 device. It is advised not to change this download location, especially if several devices are operated in parallel. Advanced users might choose a custom download folder by clicking on Change.
  • Page 55 FireStingGO2 Manager. It is recommended first to copy log files from this folder to another location, before processing them.

  • Page 56: Warnings

    FireStingGO2 (see chapter 3.5). If the FireStingGO2 is operated with the FireStingGO2 Manager software, the warnings are also shown in the Live Graph window (chapter 10.3.1). 11.1 Oxygen Sensor Warnings A reasonable oxygen sensor shows signal intensities well above 50 (typically 50-500) .

  • Page 57: Temperature Sensor Warning

    No T Sensor is shown in the top screen of the Live Mode (device) and in the Live Graph window of the FireStingGO2 Manager. While this warning is active, the device cannot provide any valid oxygen readings, as the compensation temperature is missing for calculating the oxygen value.
  • Page 58 Live Display of the FireStingGO2 Manager software in combination with the No T Sensor warning. However, the log file would still include the phase shift dphi, which is the fundamental raw data measured by the FireStingGO .

  • Page 59: Calibration Standards

    12 Calibration Standards 12.1 The Air Calibration Standard The Air Calibration standard can be  ambient air  water-vapor saturated air  air saturated water (100% air saturation) When inserting fragile needle-type oxygen sensors into the calibration standards, ensure that the sensor tips are not hitting against e.g.
  • Page 60 During the calibration of oxygen sensors, there are two possibilities to take the humidity into account: (1) The relative humidity and the temperature of the ambient air must be determined during the calibration. (2) The calibration standard is prepared in a closed vessel either filled with water or partly filled with e.g.

  • Page 61: Ambient Air

    Enclose wet cotton wool into a flask (e.g. DURAN flask) with a lid prepared with holes for the oxygen sensor and a temperature sensor from PyroScience. Typically about 1/3 to 1/2 of the flask volume is filled with the wet cotton wool, while the other volume fraction is left free for inserting the tip of the oxygen sensor, and optionally also the temperature sensor.

  • Page 62: The 0% Standard

    with an air stone connected to an air pump (available as commercial equipment for fish aquaria). Alternatively, if no air pump is available, fill water into the flask leaving >50% air in the headspace, close it with a lid and shake the flask strongly for about 1 min.

  • Page 63: Nitrogen Gas

    are completely immersed into the water and free of air bubbles. Perform the calibration as described in chapter 5.2.3. Do not store the sensors in this solution and rinse them carefully after calibration with demineralized water. Especially the retractable needle-type sensors (item no. OXR50, OXR230, OXR430) need to be rinsed very thoroughly, because salt crystallization within the needle might damage them irreversibly.

  • Page 64: Appendix

    13 Appendix 13.1 Specifications of the FireStingGO Dimensions 150 g Weight 52 x 97 x 20 mm Interface micro USB 2.0 Memory 4 GB (ca. 40 million data points) Battery rechargeable Li-Ion battery (typ. 2 h charging via micro-USB) System Requirements for Windows 7, 8, 10 FireStingGO Manager...

  • Page 65: Measuring Principle

    13.2 Measuring Principle REDFLASH technology is based on the oxygen-sensitive REDFLASH indicator showing excellent brightness. The measuring principle is based on the quenching of the REDFLASH indicator luminescence caused by collision between oxygen molecules and REDFLASH indicator immobilized on the sensor tip or surface. REDFLASH indicators are excitable with red light (more precisely: orange-red at a wavelength of 610-630 nm) and show an...

  • Page 66: Definition Of Oxygen Units

    13.3 Definition of Oxygen Units phase shift dphi The phase shift dphi is the fundamental unit measured by the optoelectronics in the FireStingGO . The measuring principle is based on a sinusoidally modulated red excitation light, resulting in a phase-shifted sinusoidally modulated emission in the NIR. This phase shift is measured and converted into oxygen units based on the Stern-Vollmer-Theory.
  • Page 67 with p100 = 0.2095 ( p – p (T) ) (T) = 6.112mbar x exp ( 17.62 T[°C] / (243.12 + T[°C])) : actual partial pressure : actual barometric pressure T: actual temperature (T): saturated water vapor pressure at temperature T Dissolved O2 concentration C µmol/L Definition:...

  • Page 68: Explanation Of The Sensor Code

    13.4 Explanation of the Sensor Code The oxygen sensors are delivered with a sensor code (written on the label attached to the sensor cable or the bag/stickers of contactless sensors), which must be entered in the Settings (see chapter 5.1.1). The following figure gives a short explanation about the information included in the sensor code.
  • Page 69 LED Intensity 100% Amplification 200x 400x C0 (Pre-Calibration at 0% O2) dphi0 = C0 / 10 C100 (Pre-Calibration at 100% O2) dphi100 = C100 / 10 The values of the pre-calibration are valid for the following calibration conditions: Partial Volume of Oxygen (% O2) 20.95 Temperature at both calibration points (°C) 20.0...

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