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pyroscience FireStingO2 User Manual

Fiber-optic oxygen & temperature meter
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FireStingO2
F
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M
IBER
OPTIC
XYGEN
EMPERATURE
ETER
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M
SER
ANUAL
1

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Summary of Contents for pyroscience FireStingO2

  • Page 1 FireStingO2 & T IBER OPTIC XYGEN EMPERATURE ETER ANUAL...
  • Page 2 Document Version 3.081 Refers to Pyro Oxygen Logger Software version >3.3 FireStingO2 is manufactured by PyroScience GmbH Hubertusstr. 35 52064 Aachen Germany Phone +49 (0)241 5183 2210 +49 (0)241 5183 2299 Email info@pyroscience.com Internet www.pyroscience.com Registered: Aachen HRB 17329, Germany...

  • Page 3: Table Of Contents

    ABLE OF ONTENT OVERVIEW ................5 SAFETY GUIDELINES ............... 7 INTRODUCTION TO THE FIRESTING METER ......10 SOFTWARE INSTALLATION ........... 12 OPTICAL OXYGEN & TEMPERATURE SENSORS ..... 13 ............ 13 VERVIEW OF ENSOR YPES ............14 ONNECTING THE ENSORS ......15 LEANING AND AINTENANCE OF THE ENSORS...
  • Page 4 OXYGEN CALIBRATION STANDARDS ........68 ..........68 ALIBRATION TANDARD 8.1.1 Ambient Air ............... 70 8.1.2 Water-Vapor Saturated Air ..........71 8.1.3 Air Saturated Water ............71 0% O ............. 72 XYGEN TANDARD 8.2.1 Water Mixed with a Strong Reductant ........ 72 8.2.2 Water Flushed with Nitrogen Gas ........

  • Page 5: Overview

    1 Overview The compact USB-powered fiber-optic meter FireStingO2 with 1, 2, or 4 channels unifies several innovative technological improvements making it the new standard of oxygen sensing with fiber-optical oxygen sensors (optodes). The FireStingO2 utilizes a measuring principle based on red light excitation and lifetime...
  • Page 6 And several customized OEM solutions are available for application in industry and underwater. More information concerning our products can be found at www.pyroscience.com or contact us at info@pyroscience.com. Your PyroScience Team...

  • Page 7: Safety Guidelines

    (optodes) from PyroScience. In order to guarantee an optimal performance of the FireStingO2, please follow these operation instructions and safety guidelines. If any problems or damage evolve, disconnect the instrument immediately, mark it to prevent any further use and consult PyroScience for repair or maintenance service.
  • Page 8 When used in the field, the environmental conditions (like high humidity, dust, exposure to direct solar radiation) may cause damage or interference of the FireStingO2, which is on the user's authority.
  • Page 9 Before using the FireStingO2 and its sensors, read carefully the instructions and user manual for the oxygen meter FireStingO2. In case of problems or damage, disconnect the instrument and mark it to prevent any further use! Consult PyroScience advice! There are no serviceable parts inside the device. Please...

  • Page 10: Introduction To The Firesting Meter

    3 Introduction to the FireSting Meter FireStingO2 is an optical oxygen meter that is compatible with a broad range of optical oxygen and temperature sensors from PyroScience:  microsensors and minisensors (retractable needle-type, fixed needle-type, or bare fiber),  robust probes and ...
  • Page 11 FireStingO2 is a laboratory instrument. Use in the field is on the user´s authority and then it is recommended to protect the FireStingO2 from heating, moisture and corrosion. FireStingO2 comes with 1, 2, or 4 channels (connectors 1 to 4) for up to 4 fiber-optic sensors and one connector (T) for an external PT100 temperature probe.

  • Page 12: Software Installation

    4 Software Installation IMPORTANT: Do not connect the FireStingO2 to your PC before Pyro Oxygen Logger software has been installed. The software will install automatically the appropriate USB-drivers. System requirements:  PC with Windows 7 / 8 / 10 (but not Windows RT) and min.

  • Page 13: Optical Oxygen & Temperature Sensors

    5 Optical Oxygen & Temperature Sensors For an overview of available optical oxygen and temperature sensor types, please see http://www.pyro-science.com/products.html 5.1 Overview of Sensor Types Sensor Available Type Analyte Application Versions Fiber-Based stirred water, gas OXROB3 Robust & semi-solid Probes OXROB10 samples water, gas &...

  • Page 14: Connecting The Sensors

    Sensor Available Type Analyte Application Versions Contactless aqueous solutions - microfluidics, Nanoprobes OXNANO cultures, enzymatic react. water & gas* - OXSP5 measurements in Sensor Spots closed containers TPSP5* Temp. with transparent window water & gas - OXVIAL4 measurements in OXVIAL20 Respiration closed vials, of Vials...

  • Page 15: Cleaning And Maintenance Of The Sensors

    ST-connectors of the FireStingO2 (1 to 4) with a male fiber plug. First, remove the black caps from the plug of the sensor / fiber. Then remove the red caps from the sensor ports at the...
  • Page 16 A signal drift of the sensor can indicate photo-bleaching of the oxygen-sensitive REDFLASH indicator or the temperature sensitive THERMOGREEN/THERMOBLUE indicator depending on the ambient light intensity, as well as the intensity of the excitation light and the sample frequency. This can necessitate a new calibration of the sensor and possibly also a re-adjustment of the Sensor Settings (LED intensity;...

  • Page 17: The Software "Pyro Oxygen Logger

    FireSting Settings window opens automatically: Activate each connected sensor in the respective channel tab of the Settings window, corresponding to the channel number at the FireStingO2 meter (see chapter 3). Enter the Sensor Code and all relevant parameters (Units, Measuring Mode, Conditions in the Sample) into each channel tab before calibration and measurements, as described in detail in chapter 6.1.

  • Page 18: Firesting Settings

    6.1 FireSting Settings Each channel (1-4) of the connected FireStingO2 meter has its own tab (channel 1-4) in the FireSting Settings window. For all optical sensors, enter first the sensor code of each connected optical sensor into the field Sensor Code in the corresponding channel panel of the Settings window.

  • Page 19: Channel Tab: Optical Oxygen Sensors

    After the first start, the dialog window FireSting Settings opens automatically. For later adjustments, it can be opened by clicking on the Settings button in the Main Window. Settings can only be adjusted if data logging is not active (see chapter 6.2.3).
  • Page 20 the mouse along the scale, thereby changing the measuring time. An intermediate mode (3) is default. NOTE: Ensure that the correct sensor code has been entered. Advanced Settings (for advanced users/applications only) If Advanced Sensor Settings are chosen, more complex setting controls get visible.
  • Page 21 : Water (Dissolved Oxygen) or Gas Phase. TEMPERATURE compensation : by - an External Temperature Sensor (PT100) connected to the temperature port of the FireStingO2, - a Fixed Temperature (needs to be determined, adjusted manually and kept constant), or Optical Temperature sensor...
  • Page 22 For Gas measurements, temperature could be measured in principal also by the Internal Temperature Sensor of the FireStingO2, but this is NOT recommended! If External Temperature Sensor, Internal Temperature Sensor (in a Gas Phase) or Optical Temperature Channel is selected, automatic compensation of temperature changes on the respective oxygen sensor readings is activated (see chapter 10.3).
  • Page 23 If the actual atmospheric pressure cannot be determined on site, it is also possible to enter the - Elevation (m) above sea level. For this click on Elevation and enter the actual elevation in meters. This procedure will only calculate the average atmospheric pressure for this elevation; therefore this option is less precise than measuring the actual atmospheric pressure.

  • Page 24: Channel Tab: Optical Temperature Sensors

    6.1.2 Channel Tab: Optical Temperature Sensors To enter the Settings for an optical temperature sensor connected FireStingO2 channel, open the respective channel tab in the FireSting Settings window of the Oxygen Logger software. Enter the Sensor Code of the connected optical temperature sensor (see label on sensor).
  • Page 25 Both temperature sensors can be activated independently even if Fixed Temperature or Optical Temperature Sensor was selected for temperature compensation of all connected oxygen sensors (in this case the oxygen measurements are not affected by these temperature sensor measurements). The measured temperature is then displayed in the Overview panel (see chapter 6.3) of the main window and saved into the data file (see chapter 6.4).

  • Page 26: Options Tab

    TeX4 can be coupled to the FireStingO2, offering 4 ports for PT100 temperature probes. 6.1.4 Options Tab In the Settings tab Options, several internal sensors and an Analog In can be activated. The Internal Pressure Sensor and the Internal...
  • Page 27 Max. Data Points in Graphs (default: 10800). A change of the number will clear the graphs and high values (>>10000) might decrease the maximum sample rate. FireStingO2 offers four analog outputs (0-2.5V) at the extension port which can be configured by pressing the Analog Output button.

  • Page 28: Main Window

    1-4 at the FireStingO2 (see chapter 3). For the 1- or 2-channel version of the FireStingO2, only the respective panels will be visible. The default sensor readings show uncalibrated sensor readings (in raw value). For oxygen sensors this gives only qualitative information of the actual oxygen level.

  • Page 29: Chart Recorder

    6.2.1 Chart Recorder The color and appearance of each graph can be changed by clicking on the color-control (CC), opening a pop-up menu. With Common Plots, Color, Line Style, Line Width, Interpolation, and Point Style the chart appearance can be changed. Clicking on the small rectangular button allows hiding / showing the respective graph.
  • Page 30 Note that placing the mouse on many elements of the window will show a short description ("tool tip"). By clicking on the right mouse button and selecting "Description and Tip" a more detailed description might be available additionally. Clear Graph The button Clear Graph offers the options to clear only the graph of one channel (Clear Single Graph), or to Clear Graphs of all Channels &...
  • Page 31 The scales of the x-axis (Time) and of the y-axes (depending on connected sensor type, see above) can be adjusted by clicking on Adjust Scales, opening the respective pop-up window: The upper (Maximum) and lower limits (Minimum) and the Increment of the Y Scales and X Scale (Time) can be changed by clicking on the respective selector or by double-clicking directly onto the field and entering the values manually (changing these parameters will automatically deactivate the auto-scaling).

  • Page 32: Warnings

    LED intensity and / or the amplification in the Advanced Settings (see chapter 6.1.1) Bad reference  This indicates internal problems of the FireStingO2. Please contact PyroScience for support. NOTE: Do not continue with measurement if a warning is shown! Note: Exceptions are trace oxygen sensors.

  • Page 33: Measurement And Logging

    Refer also to the Troubleshooting in chapter 12.3. The actual Compensation Temperature (see chapter 6.1.1), i.e. the temperature used for calculating the oxygen values, is shown in the temperature display (TD) in units of degree Celsius (°C). When no external PT100 temperature sensor is connected, but activated in the Settings (see chapter 6.1), NaN is displayed and a warning Bad Temperature appears below the chart recorder (also for broken or nonfunctional temperature sensors).
  • Page 34 progress. Clicking again on it will stop the measurement (dark green arrow). The mode of Measurement can be chosen as single data point acquisition, continuous sampling (default setting) or as continuous sampling limited to a defined time interval. The duration of this time interval can be adjusted in the duration display (DUD) shown as hour (HH): minutes (MM): seconds (SS).
  • Page 35 Data Logging To activate data logging, click on the red start button (SB) of Log to File. Select a file name in the appearing file dialog. The saved data files are simple text-files with the file extension ".txt", which can be easily imported into common spreadsheet programs. Thereafter, the indicator Comment is shown additionally.
  • Page 36 Load Setup. This allows e.g. to switch between different laboratory setups with a single FireStingO2. This function might be also useful if different computers are used for the calibration and for the actual measurements. You might calibrate the sensors with the first computer, save the configuration with Save Setup.

  • Page 37: Raw Data Window

    FireStingO2. This allows measurements in different setups at the same time. The flashing of the logo (for ca. 1 sec after pressing the Flash Logo button) can help to assign a specific logger window to the corresponding FireStingO2 meter (more details in chapter 12.5).
  • Page 38 (Internal Temp. (°C)) in the FireStingO2 meter, the Pressure (mbar) and Humidity (%) measured by the internal sensors inside the FireStingO2, as well as the Analog In (mV) are also displayed. On the left side, the Status and different warnings can be...
  • Page 39 NOTE: While the Raw Data window is opened, all raw values are also saved into the data file in additional columns behind the standard data columns On the right-hand side of the channel tabs, a graph can be activated, showing the dphi (°) and Signal Intensity (mV) in the graph (default setting).

  • Page 40: Overview Panel

    6.3 Overview Panel In the panel Overview, the sensor readings of all activated oxygen sensors and the signals from the Analog In are displayed on the left y-axis. The readings of the optical, external and internal temperature sensors and of the internal pressure and humidity sensors are displayed on the right y-axis.

  • Page 41: Data File Panel

    6.4 Data File Panel In the Data File Panel, the current log file is shown and can be exported to Excel. By clicking on the Update Table button, the latest data are displayed in the table. By clicking on Export to Excel, the data file is exported to Excel®.

  • Page 42: Sensor Calibration

    "Pyro Oxygen Logger". Please note that the calibration modes described in this chapter can be only performed with a FireStingO2 device with firmware version >3.0 (having integrated humidity and pressure sensors) and a software version >3.3 of the Oxygen Logger.

  • Page 43: Optical Oxygen Sensor Calibration

    7.1 Optical Oxygen Sensor Calibration Before starting the calibration, ensure that the correct Sensor Code has been entered in the settings (refer to chapter 6.1.1) and prepare appropriate calibration standards (refer to chapter 8). For calibration of contactless sensors, please refer also to chapter 9. To calibrate a sensor click on the button Calibrate in the corresponding channel panel.
  • Page 44 saturated air (100% RH, see chapter 8.1.2) for precise measurements around 100% air saturation. 2-Point in Ambient Air: taking the 0% and the air calibration value from a manual calibration for precise measurements over the full range (0-21% O2). This mode uses the ambient air (see chapter 8.1.1) for determining the air calibration value.

  • Page 45: Calibration Mode: Factory

    For advanced users and applications, a Custom Mode can be selected, allowing the user to combine freely the possible calibration types for the air and the 0% calibration. Possible Air Calibration Types in the Custom Mode:  Air with 100% Humidity or Air Saturated Water, ...

  • Page 46: Calibration Mode: 1-Point In Ambient Air

    If the calibration mode Factory Calibration is chosen, ensure that the correct sensor code has been entered in the Settings of the corresponding channel (as displayed in 2. Adjust Calibration Conditions of the Oxygen Sensor Calibration window). If the sensor code displayed is not correct, click on Finish, go to the Settings, enter the correct Sensor Code and repeat the Factory Calibration.
  • Page 47 If the atmospheric pressure and the relative humidity of the ambient air are read from the internal Pressure and Humidity sensors, it is important that both the FireStingO2 device and the connected oxygen sensor are exposed to identical environmental conditions.
  • Page 48 FireStingO2. And even worse, the evaporation of water drops would cool down the sensor tips causing undefined temperatures. It is recommended that the device and the sensor are placed for >30 min.

  • Page 49: Calibration Mode: 1-Point In Water Or Humid Air

    Finally, click on Finish for returning to the main window. NOTE: If using retractable needle-type sensors (e.g. OXR50, OXR230, (TR)OXR430 or TPR430), it is important that the sensor tip is extended out of the needle when the calibration value is taken.
  • Page 50 Sensor, it is important that the oxygen sensor in the calibration standard (air saturated water or water-vapor saturated air) is exposed to the same atmospheric pressure as the FireStingO2 device (which is given in typical applications For compensation of the Temperature in the calibration standards...

  • Page 51: Calibration Mode: 2-Point In Ambient Air

    Wait now until the sensor reading is stable by observing the graph and the numerical display of the oxygen sensor reading. If External or Optical Temperature Sensor was selected, ensure also stable temperature readings indicated at Compensation Temperature (°C). Note that the button Set Air will be highlighted as soon as the oxygen readings are within the expected range for the connected sensor type.
  • Page 52 If the atmospheric pressure and the relative humidity of the ambient air are read from the internal Pressure and Humidity Sensors, it is important that both the FireStingO2 device and the connected oxygen sensor are exposed to identical environmental conditions.
  • Page 53 For the air calibration value (Ambient Air, see chapter 8.1.1), place the oxygen sensor and the external/optical temperature sensor (if used) close to the air holes at the backside of the FireStingO2. Ensure that the oxygen sensor and the temperature sensor (if used) are completely dry;...
  • Page 54 (1) Fixed Temperature can be adjusted manually (needs to be determined and kept constant) or the temperature is read from an (2) External (PT100) Temperature Sensor connected to the temperature port of the FireStingO2 or from an (3) Optical Temperature Sensor connected to a channel at the FireStingO2 meter (its respective channel number needs to be entered at Optical Temp.

  • Page 55: Calibration Mode: 2-Point In Water Or Humid Air

    Note that the button Set 0% will be highlighted as soon as the oxygen readings are within the expected range for the connected sensor type. If all readings have reached their steady-state, click on Set 0%, and the actual oxygen sensor reading is taken for the 0% calibration. If the oxygen reading seems to be out of the expected range, a warning will be shown offering the possibility to repeat the calibration (it is not recommended to continue without checking...
  • Page 56 Sensor, it is important that the calibration standards (air saturated water or water-vapor saturated air, de-oxygenated water) are exposed to the same atmospheric pressure as the FireStingO2 device (which is given in typical applications For Temperature compensation during oxygen sensor calibration...
  • Page 57 (3) Optical Temperature Sensor connected to a channel at the FireStingO2 meter (its respective channel number needs to be entered at Optical Temp. Channel). Set Air For the air calibration point (Air with 100% Humidity or Air Saturated Water), insert the oxygen and temperature sensor into the flask containing either water-vapor saturated air (see 8.1.2) or...
  • Page 58 (1) Fixed Temperature can be adjusted manually (needs to be determined and kept constant) or the temperature is read from an (2) External (PT100) Temperature Sensor connected to the temperature port of the FireStingO2 or from an (3) Optical Temperature Sensor connected to a channel at the FireStingO2 meter (its respective channel number needs to be entered at Optical Temp.

  • Page 59: Calibration Mode: Custom Mode

    NOTE: If using retractable needle-type sensors (e.g. OXR50, OXR230, (TR)OXR430 or TPR430), it is important that the sensor tip is extended out of the needle when the calibration value is taken. 7.1.6 Calibration Mode: Custom Mode The custom calibration mode is selected by clicking on the button Custom Mode.
  • Page 60 If the atmospheric pressure and the relative humidity of the ambient air are read from the internal Pressure and Humidity Sensors, it is important that both the FireStingO2 device and the connected oxygen sensor are exposed to identical environmental conditions. Especially the use of the internal humidity sensor...
  • Page 61 (1) Fixed Temperature can be adjusted manually (needs to be determined and kept constant) or the temperature is read from an (2) External (PT100) Temperature Sensor connected to the temperature port of the FireStingO2 or from an (3) Optical Temperature Sensor connected to a channel at the FireStingO2 meter (its respective channel number needs to be entered at Optical Temp.
  • Page 62 Consequently, the 0% calibration should be performed. If the calibration type 0% Factory Calibration was selected no further steps are necessary (refer also to chapter 7.1.1). If 0% calibration was selected, follow the instructions given in chapter 7.1.4. Click on Finish for reverting to the main window. NOTE: If using retractable needle-type sensors (e.g.
  • Page 63 This section is only for advanced users! For very advanced applications it is possible to manipulate all internal calibration parameters of the FireStingO2 manually. This option is accessible by selecting Custom Mode and subsequently clicking on Advanced: Adjust Manually, which opens a separate window showing all internal calibration parameters.
  • Page 64 These parameters are specific constants for the REDFLASH indicators, and are automatically adjusted for the selected Sensor Type in the settings. Unless otherwise communicated by PyroScience, it is strongly advised to leave these parameters at their default values.

  • Page 65: Optical Temperature Sensor Calibration

    Note that during data logging this button cannot be used until Log to File was stopped. In the dialog window Optical Temperature Calibration two calibration modes can be selected for 1-point calibration of the optical temperature sensor connected to a channel at the FireStingO2 device:  Calibrate with External Temp. ...
  • Page 66 For a calibration with the external temperature sensor (item no. TDIP15 or TSUB21), first check for a temperature offset of the connected PT100 sensor (see chapter 6.1.3). After adjusting this manual offset, the 1-point calibration of the optical temperature sensor can be performed subsequently in its calibration window. Ensure that the external temperature sensor is placed close to and exactly to the same temperature conditions as the optical temperature sensor.
  • Page 67 Note: It is strongly recommended to perform a calibration at conditions close to the environmental conditions during measurements. Ensure constant conditions during calibration! Wait until the temperature sensor readings are stable by observing the graph and the numerical display of the Optical Temperature (°C) sensor.

  • Page 68: Oxygen Calibration Standards

    8 Oxygen Calibration Standards 8.1 The Air Calibration Standard The Air Calibration standard can be  ambient air  water-vapor saturated air  air saturated water (100% air saturation) When inserting fragile needle-type oxygen sensors into the calibration standards, ensure that the sensor tips are not hitting against e.g.
  • Page 69 During the calibration of oxygen sensors, there are two possibilities to take the humidity into account: (1) The relative humidity and the temperature of the ambient air must be determined during calibration. The Pyro Oxygen Logger software calculates then automatically the real oxygen level under these conditions.

  • Page 70: Ambient Air

    And in case of a second generation FireStingO2 (with micro USB connector), the built-in humidity and pressure sensors together with the internal or external temperature sensor will measure these parameters automatically for most calibration types (see also chapter 7).

  • Page 71: Water-Vapor Saturated Air

    Enclose wet cotton wool into a flask (e.g. DURAN flask) with a lid prepared with holes for the oxygen sensor and a temperature sensor from PyroScience. Typically about 1/3 to 1/2 of the flask volume is filled with the wet cotton wool, while the other volume fraction is left free for inserting the tip of the oxygen sensor, and optionally also the temperature sensor.

  • Page 72: The 0% Oxygen Standard

    8.2 The 0% Oxygen Standard The 0% calibration standard can be  water mixed with a strong reductant  water flushed with nitrogen gas (N  nitrogen gas (N 8.2.1 Water Mixed with a Strong Reductant Fill an appropriate amount of water into a glass flask (e.g. Duran flask) with a lid prepared with holes for inserting the oxygen sensor and a temperature sensor.

  • Page 73: Water Flushed With Nitrogen Gas

    8.2.2 Water Flushed with Nitrogen Gas Fill water into a glass flask (e.g. Duran flask) with a lid prepared with holes for inserting the oxygen sensor and a temperature sensor. Close it and stream for about 10 minutes nitrogen gas through the water.

  • Page 74: Calibration Of Contactless Sensors

    (experienced by the FireStingO2). A falsely determined relative humidity gives a maximum relative error of 1% for the air calibration (refer also to chapter 8.1).

  • Page 75: Manual Background Compensation

    FireStingO2 with the contactless oxygen sensor. Based on the Fiber Length (m) entered in the Settings (see chapter 6.1.1), a background signal for compensation is estimated...
  • Page 76  one end of the Optical Fiber is connected to the corresponding channel of the FireStingO2  the other end of the Optical Fiber attached to the sensor spot (i.e. disconnect this end from the spot adapter, adapter ring or from the flow-through cell) Then wait for steady-state and press the button Take Actual Values.

  • Page 77: Temperature Measurement

    PT100 temperature sensors refer to chapter 12.1 and for the optical temperature sensors refer to their website: https://www.pyro-science.com/products.html 10.1 External (PT100) Temperature Sensors FireStingO2 provides one port for an external temperature sensor. PyroScience offers e.g. the dipping-probe temperature...

  • Page 78: Optical Temperature Sensors

    Temperature Sensor connected to the FireStingO2 or with an external thermometer. 10.3 Optical Temperature Sensors For precise temperature measurements and true automatic temperature compensation of the oxygen measurements, it is recommended to use optical temperature sensors for automatic temperature compensation. The optical temperature sensors are connected to the same ports as the optical oxygen sensors.
  • Page 79 TeX4. For true temperature compensation it is recommended to choose an optical temperature sensor connected to a channel of the FireStingO2. However, it is also possible to use the automatic temperature compensation e.g. only for a single oxygen channel, whereas the other oxygen channels can be used for measurements at a Fixed Temperature.
  • Page 80 authority to ensure that the sample is kept under this fixed temperature during the measurements. Even if measurements with all activated oxygen sensors are performed under a Fixed Temperature, the external and optical temperature sensor can be used for independent temperature measurements.

  • Page 81: Analog Output And Auto-Mode

    FireStingO2 via the USB interface (as described in this manual). This user-friendly operation mode is generally recommended, as it offers easy control over the full functionality of the FireStingO2. However, several advanced features are available in addition for integrating the FireStingO2 in customized setups.
  • Page 82 FireStingO2 performs a new measurement (independent whether FireStingO2 is operated via a PC with the Pyro Oxygen Logger software, or if it is operated autonomously in the auto-mode). The analog output can be configured by opening the Settings window clicking...
  • Page 83 voltage signals proportional to the measured value. The linear scaling of the voltage signal can be freely adjusted at 0 mV output corresponds to and 2500 mV output corresponds to. NOTE: Due to hardware restrictions, the very lower range of the analog output around 0-3 mV shows slight non-linearities.

  • Page 84: Auto-Mode

    NOTE: Some older micro-USB-charger do not fulfill the new common standard for micro-USB-chargers. Such an old micro- USB-charger might not be recognized by the FireStingO2, and thus the auto-mode is not started. This can be checked by observing the flashing frequency of the logo.
  • Page 85 Here the Auto-Mode Sample Interval (s) can be adjusted, defining the time interval between consecutive measurements in the auto- mode. As an advanced feature, the option Enable Data Transmission activates digital data transmission via the UART interface of the extension port (details on request). NOTE: Only sensors (e.g.

  • Page 86: Advanced Auto-Mode

    11.3 Advanced Auto-Mode NOTE: This section is only for advanced users! For advanced applications, the auto-mode can be configured even more flexible as described in the preceding chapter. First, it is possible to define an independent sample interval for each oxygen channel;...
  • Page 87 respective channel is only performed during the auto-mode if a trigger signal was detected at the trigger input of the extension port (see appendix 12.2). By checking Enable Serial Port Data Transmission, the results for the respective channel are additionally transmitted via the UART interface of the extension port.

  • Page 88: Appendix

    12 Appendix 12.1 Specifications of the FireStingO2 Dimensions 68 x 120 x 30 mm Weight 350 g Interface USB 2.0 Power Supply USB-powered (max 70mA at 5V) Supported operating systems Windows 7,8, 10 (but not Windows RT) Operating temperature 0 to 50ºC Max.
  • Page 89 Analog Output (4 channels) 0 to 2.5 VDC, 14 bit resolution Note: slight non-linearities around 0..5mV at extension port X2 Connector plug Phoenix Contact item no. 1778887 for extension port X1 Connector plug Phoenix Contact item no. 1778861 for extension port X2 Digital interface UART with 3.3V levels at extension port X1...

  • Page 90: Extension Port X1

    12.2 Extension Port X1 The extension port of the FireStingO2 consists of the two connectors X1 and X2 (fitting connector plugs can be obtained from Phoenix Contact item no. 1778887 and 1778861). 12.2.1 Connector X1 (Power, Digital Interface, Analog In) The pin configuration of the connector X1 is given in the table below.
  • Page 91 Name Function Description Power Ground Power Power supply, 3.5V to 5.0V DC max. 70 mA (typ 40 mA) /USB_DISABLE Disables USB interface Ground Digital Output Data transmission pin (0V or 3.3V) of the UART interface Digital Input Data receive pin of the (0V or 3.3V) UART interface (5V tolerant)
  • Page 92 The following illustrations show typical use cases for the extension port X1: USB-Powered Auto-Mode FireStingO2 is powered e.g. by a USB connection to a PC. By closing the blue switch between pin 6 and pin 1, the auto-mode is activated. Note, if the...
  • Page 93 1 and 2. The USB connector of the FireStingO2 is left unconnected. Note the additional connection between pin 1 and 3 powering down the internal USB interface of the FireStingO2. Full Control Mode via the UART Interface...
  • Page 94 (OEM applications). Note the additional connection between pin 1 and 3 powering down the internal USB interface of the FireStingO2. Otherwise unintended data communication via a potentially still connected PC might disrupt the UART communication. More information is available on request.

  • Page 95: Connector X2 (Analog Output)

    12.2.2 Connector X2 (Analog Output) The connector X2 provides 4 independent analog outputs with a range of 0-2.5V DC at a resolution of 14 bits (see table below). Refer to chapter 11.1 how to configure the analog outputs. Name Function Description Ground AO_A...

  • Page 96: Troubleshooting

    Sensor signal is too low: → check whether the sensor cable is connected → increase LED Intensity in the Advanced settings → replace sensor, the tip might be broken / bleached Bad Reference Internal problem of the electronics → contact PyroScience...

  • Page 97: Oxygen Measuring Principle

    12.4 Oxygen Measuring Principle The new REDFLASH technology is based on the unique oxygen- sensitive REDFLASH indicator showing excellent brightness. The measuring principle is based on the quenching of the REDFLASH indicator luminescence caused by collision between oxygen molecules and the REDFLASH indicator immobilized on the sensor tip or surface.
  • Page 98 The measuring principle is based on a sinusoidally modulated red excitation light. This results in a phase-shifted sinusoidally modulated emission in the NIR. The FireStingO2 measures this phase shift (termed "dphi" in the software). The phase shift is then converted into oxygen units based on the Stern-Vollmer-Theory.

  • Page 99: Operating Several Firestingo2 In Parallel

    70 mA); an external power supply for the USB-hub might be advisable. Pyro Oxygen Logger software has now to be started separately for each connected FireStingO2. So, if you want to operate e.g. 6 different FireStingO2 meters, you have to start the...

  • Page 100: Definition Of Oxygen Units

    Used in: gas and water phase For a calibrated sensor, the partial oxygen pressure p in units of hPa (equivalent to mbar) is the fundamental oxygen unit measured by the FirestingO2. partial pressure p Torr Definition: [Torr] = p [hPa] x 759.96 / 1013.25...
  • Page 101 % air saturation A % a.s. Definition: A[%a.s.] = 100% x p / p100 Used in: water phase with p100 = 0.2095 ( p – p (T) ) (T) = 6.112mbar x exp ( 17.62 T[°C] / (243.12 + T[°C])) : actual partial pressure : actual barometric pressure T: actual temperature...

  • Page 102: Table Of Oxygen Solubility

    12.7 Table of Oxygen Solubility The following Table shows the equilibrium oxygen concentration (T, P=1013mbar, S) in units of µmol/L at standard atmospheric pressure of 1013 mbar as a function of water temperature in units of °C and salinity in units of PSU (“practical salinity unit” ≈ g/L). In order to correct these values for the actual atmospheric pressure , the following formula has to be applied: (T,P,S) = C...
  • Page 103 Temp (°C) (PSU) 456.6 398.9 352.6 314.9 283.9 257.9 235.9 217.0 200.4 450.4 393.6 348.1 311.1 280.6 255.0 233.3 214.7 198.3 444.2 388.5 343.7 307.3 277.3 252.1 230.8 212.4 196.3 438.1 383.3 339.4 303.6 274.0 249.3 228.3 210.2 194.3 432.1 378.3 335.1 299.9...

  • Page 104: Explanation Of The Sensor Code

    12.8 Explanation of the Sensor Code The oxygen sensors are delivered with an attached sensor code which must be entered in the Settings (refer to chapter 6.1). The following figure gives a short explanation about the information given in the sensor code. XB7-532-205 Example Code: Sensor Type...
  • Page 105 LED Intensity 100% Amplification 200x 400x Oxygen Sensors C0 (Factory Calibration at 0% O2) dphi0 = C0 / 10 C100 (Factory Calibration at 100% O2) dphi100 = C100 / 10 The values of the factory calibration are valid for the following calibration conditions: Partial Volume of Oxygen (% O2) 20.95...

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