TANNER Scientific TN50 Operating Instructions Manual page 27

8.12 TIPS AND TRICKS
It is common knowledge that the temperature of the cryostat plays an important role in
the quality of the tissue sections obtained. Each type of tissue needs a different
temperature as shown in the following list. The temperature ranges indicated in the
table below are approximate values and may require adjustments for individual tissues.
Recommended temperature range at
Type of Tissue
the blade edge [°C]
Bone marrow
Brain
Breast with fat
Breast without fat
Cartilage
Fat
Heart
Intestinal
Kidney
Larynx
Lip
Liver
Lung
Lymph-Node
Lymphatic
Lymphoid
Muscle
Nose
Rectal
Scrapings
Skin with fat
Skin without fat
Spleen
Testicle
Tongue
Uterine-
Operating Instructions – TN50
-16 to -25
-7 to -10
-25 to -30
-16 to -20
-13 to -20
-30 to -40
-20 to -25
-13 to -20
-13 to -20
-13 to -16
-10 to -20
-7 to -13
-13 to -20
-13 to -20
-13 to -20
-13 to -20
-13 to -20
-13 to -20
-13 to -20
-16 to -25
-16 to -25
-10 to -16
-7 to -10
-10 to -13
-13 to -20
-7 to -10
For tissue which has to
i
be cut considerably
below normal chamber
temperature (e.g. fatty
tissues) and in order to
avoid cooling artefacts,
an additional bench top
CO freezer is available.
2
Exceptionally freezing
spray can be used.
27