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TANNER Scientific TN50 Operating Instructions Manual

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OPERATING
INSTRUCTIONS
INS2000GB
2018-10-003.00
Cryostat
TN50

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Summary of Contents for TANNER Scientific TN50

  • Page 1 OPERATING INSTRUCTIONS Cryostat TN50 INS2000GB 2018-10-003.00...
  • Page 2 Operating Instructions – TN50...

  • Page 3: Table Of Contents

    ............14 8. OPERATION OF MICROTOME ............20 9. OPERATION OF CRYOSTAT ............28 10. CLEANING AND MAINTENANCE ............33 11. SERVICE ............37 12. OPTIONAL ACCESSORIES ............38 13. WARRANTY ............39 14. DISPOSAL ............39 Operating Instructions – TN50...

  • Page 4: Intended Use

    1. INTENDED USE The floor standing open top cryostat TN50 is intended for rapid freezing and cutting thin sections of frozen specimens for professional use in routine and research laboratories in the fields of biology, medicine and industry. The system of knife advance operates very reliable from 0.5 to 100 µm with trimming function up to 750 µm.
  • Page 5 The hand wheel can be stopped in any position. For this purpose move the stop lever towards the centre. Hand wheel stop released. Movement. To release the stop please turn the lever towards the outside again. Hand wheel stop activated. No movement. Operating Instructions – TN50...
  • Page 6 3.2 FINGER PROTECTION Always use the finger protection (red lever)  Before you start any work with the blade or specimen  Before changing the specimen  During work break Finger protection released. Finger protection activated. Operating Instructions – TN50...
  • Page 7 Use a container that has guides to hold the blades rigid. Never try to catch a falling disposable blade. Always insert the specimen first and then the disposable blade. To avoid compression or knife marks, ensure that the blade is clean. Operating Instructions – TN50...

  • Page 8: Components

    4. COMPONENTS The Tanner Scientific open top cryostat TN50 is provided with the following standard components: TN50  Basic unit with refrigeration system  Semi-automatic rotary microtome  Operation manual  Brush clear-cut  Anti-roll plate Ready to use ...

  • Page 9: Specifications

    -5 °C chamber temperature Freezing bar Minimal temperature 10 K lower than chamber temperature Number of positions 24 (standard version) resp. 21 + 2 (version with quick freeze places) Quick freeze positions 2 (optional), to -55 °C (Peltier cooling) Operating Instructions – TN50...
  • Page 10 8° (x and y axes), 360° (z axes) Cryostat Dimensions (L x W x H) 680 mm x 760 mm x 1110 mm 27” x 30” x 44” Weight (without accessories) 127 kg / 280 lbs Operating Instructions – TN50...

  • Page 11: Unpacking And Installation

    Extend the two front feet to stabilize the cryostat in its position by turning the supporting screws counter clockwise. For repacking use the original cases. Keep the packing material. Operating Instructions – TN50...
  • Page 12 6.2 INSTALLATION SITE REQUIREMENTS The site for installation for TN50 should meet the following requirements:  A maximum ambient temperature of 22°C  No direct sunlight into the cooling chamber  Mains power supply within 3 m  No air circulation (for example by air conditioning) ...
  • Page 13  For folding the lever back, pull the horizontally orientated lever and fold it away. Hand wheel lever in folded position. Hand wheel lever unfolded. Operating Instructions – TN50...

  • Page 14: Initial Operation

    Cha: -20 °C (-22) QF: 0 quick-freeze unit (optional). 7.2 CONTROL PANEL Software settings and motor operation can be operated with the control panel on the left to side of the instrument. TN50 Control Panel. Operating Instructions – TN50...
  • Page 15 Retraction 5 µm Change entries by pressing Start defrost Store new settings by pressing Press to return to the main menu. CUT: 7 µm Trim: 19µm Cha: 22 °C (25) QF: 0 Operating Instructions – TN50...
  • Page 16 Select temperature increase end and set a ending Disinf. delay 10 h time for the automatic power saving mode. Incr. start 21:00 (e.g. end at 04:00 h in this example) > Incr. end 04:00 _ _ _ _ _ 5 6 Incr. days Operating Instructions – TN50...
  • Page 17 Incr. end 04:00 for 24 hours. > Incr. days _ _ _ _ 5 6 _ (e.g. Fridays and Saturdays in this example) 1-Monday 2-Tuesday 3-Wednesday 4-Thursday 5-Friday 6-Saturday 7-Sunday Operating Instructions – TN50...
  • Page 18 ON / OFF / AUTO The light can be set on or off. If AUTO has been selected, the light will switch off after the time being set under Light duration. Light duration 0 - 99 minutes Operating Instructions – TN50...
  • Page 19 Time A language can be selected out of a list of available Language languages. Display version Display of software version. Upon selecting Program settings the automatic Program settings defrost and power saving settings can be changed. Operating Instructions – TN50...

  • Page 20: Operation Of Microtome

    3 Loosen the blade fixation by turning the right lever counter clockwise. 4 Remove/Insert blade from one side. Loosening of blade fixation and insertion of new blade. Tighten the blade fixation by turning the right lever clockwise. Fixation of blade. Operating Instructions – TN50...
  • Page 21 (counter clockwise turn of the knob). Tighten the blade holder fixation by turning the right lever on the blade holder base clockwise. Release hand wheel lock for cutting. Fixation of blade holder with increased cutting angle. Operating Instructions – TN50...
  • Page 22 Please pay attention, that under all circumstances during operation, the fixation lever must not point downward, otherwise there is a risk of collision and damage. Release of object holder fixation; removal and/or insertion of object holder. Operating Instructions – TN50...
  • Page 23 Fix the sample orientation by turning the fixation lever on the left side of the sample holder clockwise. Release the handwheel lock to cut the samples. Fix the sample orientation. Levers must be up! Operating Instructions – TN50...
  • Page 24 QF: 0 Activation of trimming function is displayed as illustrated. If Trim latching mode is deactivated, trimming function is activated by continuously pressing Activation of trimming function is displayed as illustrated above. Turn hand wheel evenly clockwise Operating Instructions – TN50...
  • Page 25 Leave hand wheel with handle at its lowest point (specimen holder underneath the disposable blade). Transfer the section onto a slide. Leave brush always in the chamber to keep it cold. Operating Instructions – TN50...
  • Page 26 Horizontal adjustment of anti-roll plate For height adjustment of the anti-roll-plate, it is recommended to adjust the correct height during cutting. Operating Instructions – TN50...
  • Page 27 Rectal -13 to -20 Scrapings -16 to -25 Skin with fat -16 to -25 Skin without fat -10 to -16 Spleen -7 to -10 Testicle -10 to -13 Tongue -13 to -20 Uterine- -7 to -10 Operating Instructions – TN50...

  • Page 28: Operation Of Cryostat

    UV light without the addition of chemicals. The cryostat TN50 can be equipped with a UVC light source that can either request activation in defined periods (see section 7.3) or is manually activated.
  • Page 29 Picture 6: Picture 7: Once the plug is connected to the UV-lamp, the final step can be done now and the UV-lamp unit can clip into the retaining clips. Operating Instructions – TN50...
  • Page 30 9.2 QUICK FREEZE POSITION [only applicable to instruments equipped with this feature] For convenience of the user, the cryostat TN50 can be equipped with a quick freeze shelf with two positions located in the object freezing shelf. Upon activation the installed peltier element will cool down the two quick freeze position to approximately - 55 °C.
  • Page 31 Before switching on the instrument ensure that the instrument is completely dry, otherwise ice built up can damage mechanical parts During defrosting (automatic or manual) always leave front window open. Operating Instructions – TN50...
  • Page 32 The contents of the condensate bottle should be disposed according to laboratory regulations. To reduce the risk of contamination and infection, after emptying, is recommended to fill a disinfectant concentrate into the condensate bottle.. Operating Instructions – TN50...

  • Page 33: Cleaning And Maintenance

    Do not turn the instrument on before the cryochamber and the microtome are completely dry otherwise ice built up can damage mechanical parts. All components removed from the cryostat must be carefully dried before returning them to the cryochamber! Operating Instructions – TN50...
  • Page 34 Check of temperature sensor in cryochamber. Cleaning of cryostat ventilation (see section 10.4). Complete Service Yearly (performed by authorized Tanner Scientific service technician)  check of all functions  complete defrost  removing of microtome  oiling of movable parts ...
  • Page 35 Remove the pressure plate and clean the blade holder with a brush or cloth Remove the pressure plate Reassemble the pressure plate and the lever in reverse order and release the handwheel lock for cutting. Operating Instructions – TN50...
  • Page 36 8 screws (four on each side). Remove the dust with a vacuum cleaner. A cleaning of the cryostat ventilation is recommend-ded monthly. We suggest using a vacuum cleaner and a brush for cleaning. Operating Instructions – TN50...

  • Page 37: Service

    It must be returned in its original packing. If the instrument or parts thereof are sent back in a dirty or non-disinfected condition, Tanner Scientific reserves the right to return the parts to the debit of the customer. Operating Instructions – TN50...

  • Page 38: Optional Accessories

    12. OPTIONAL ACCESSORIES Disposable Blade Holder (low profile) Disposable Blade Holder (high profile) 2x Quick freezing positions UV disinfection Heat extractor block Cork plate (100 pcs.; Ø 20 mm) Brush clear-cut Anti-roll plate (disposable blade holder) Operating Instructions – TN50...

  • Page 39: Warranty

    13. WARRANTY Tanner Scientific guarantees that the product delivered has been subjected to a comprehensive quality control procedure, and that the product is faultless and complies with all technical specifications and/or agreed characteristics warranted. Tanner Scientific guarantees that the instrument is manufactured under an ISO 9001 quality management system.
  • Page 42 Tanner Scientific 12210 Rangeland Parkway 34211 Bradenton FL / US Tel. (888) 708-5233 sales@tannerscientific.com http://www.tannerscientific.com...

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