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Sakura Tissue-Tek Xpress x120 Quick Reference Manual
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Sakura Tissue-Tek Xpress x120 Quick Reference Manual

Continuous rapid tissue processor
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Quick Reference Guide
Tissue-Tek® Xpress® x120
Start Up
The x120 can be programmed to automatically
initiate a four-hour system warm up so it is ready
for operation when the workday begins.
If the PPS On-Board function software is used make
sure the User Guide x120 On-Board PPS Timer
function is used as leading for System Operation.
Run preparation for processing
Pre-Start Checks
1.
Loading Station is filled with Pre-Processing
Solution and replaced every 4 hours, or filled
with Molecular Fixative and refreshed daily
depending on the software version.
2.
No baskets are loaded in the instrument.
3.
Transfer arm is free for moving.
4.
All retort lids are closed.
5.
The basket holds up to 40 cassettes but should
not exceed the maximum limit rim.
6.
Do not use cassettes that contain any metal
which can interfere with microwave; it can
cause small burning artifacts in the tissue, and
can as well damage the microwave oven.
Sakura Finetek Europe B.V. | Flemingweg 10a, 2408 AV Alphen a/d Rijn | NL
Phone: +31 (0) 88 592 00 00 | Fax: +31 (0) 88 592 00 01 | sakura@sakura.eu | www.sakura.eu
Loading station filled with Pre-
Processing Solution or
Molecular Fixative.
Access permitted Icon and loading indicator is illuminated
Basket, holds up to 40
cassettes

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Summary of Contents for Sakura Tissue-Tek Xpress x120

  • Page 1 Sakura Finetek Europe B.V. | Flemingweg 10a, 2408 AV Alphen a/d Rijn | NL Phone: +31 (0) 88 592 00 00 | Fax: +31 (0) 88 592 00 01 | sakura@sakura.eu | www.sakura.eu...

  • Page 2: System Operation

    Quick Reference Guide Tissue-Tek® Xpress® x120 System Operation System operation is comprised of three phases: Loading Tissue Specimens (phase 1): Verify if the Access Permitted Icon and the Loading Indicator are illuminated. Log on to the instrument. Open the loading station door and remove the lid from the loading station container.
  • Page 3 Pre-Processing step inside the Tissue- ® ® Xpress x120. Handling of the Pre-Processing Solution will be automated and standardized. Sakura Finetek Europe B.V. recommended protocol: all tissue types are immersed for 15 minutes in Pre-Processing Solution (PPS) loading station.
  • Page 4 Quick Reference Guide Tissue-Tek® Xpress® x120 Loading Station Icon & Solution The conditions for the Loading Station Icon Colors are as follows: Expiration Message Blue – Set Solution Expiration Time has not countdown reaches SOLUTION expired. EXPIRATION TIME PREPROCESSING Yellow – Set Solution Expiration Time has expired SETTINGS, the message window in the bottom and needs to be replaced.
  • Page 5 Quick Reference Guide Tissue-Tek® Xpress® x120 Loading Door Icon & TIME LEFT A basket is transferred to Station #1. It is possible to load a When the Loading Station is empty and if the program can satisfy basket, but the basket will stay in the Loading Station longer than the set preprocessing time, the “Green OK”...
  • Page 6 Quick Reference Guide Tissue-Tek® Xpress® x120 Time left display when PRE- PROCESSING SETTINGS are disabled The time displayed in TIME LEFT for the Loading Station indicates the waiting time required until the basket can be transferred to Station 1 after setting a basket into the Loading Station (in other words, the detention time of the basket in the Loading Station).

  • Page 7: Maintenance

    Quick Reference Guide Tissue-Tek® Xpress® x120 Maintenance After every run 1. Basket, magazines, lids and handles must cleaned once unloaded from instrument. Paraffin residues clog tubing. a. With the use of an industrial dish washer (temperatures ≤100°C). b. Cleaning cycle overnight Pipette processor as VIP-series.

  • Page 8: Service & Maintenance

    Overflow bottles Reprocessing of Tissue Tissue-Tek Xpress x120-processed tissue may be reprocessed, if necessary, without adverse effects on the tissue. Tissue that has been trimmed too thick for proper processing can be reprocessed using the following reprocessing procedure.
  • Page 9 Quick Reference Guide Tissue-Tek® Xpress® x120 Recovery process (section 5, Operating Manual) The recovery procedure enables the operator to abort and restart any basket that may have been suspended in station 3 and/or 4. Write down the displayed message. Note the time left for the basket in station 3 and/or 4.
  • Page 10 Quick Reference Guide Tissue-Tek® Xpress® x120 Do’s and Don’ts DO NOT use blue sponges for tissue processing. DO use embedding bags or biopsy cassettes (no mesh cassettes) DO NOT use metal lids on cassettes for processing DO run RNA tissue after the processor is cleaned DO NOT process tissue thicker than 2 mm on the Standard program.