Sakura Tissue-Tek Xpress x120 Operating Manual
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Sakura Tissue-Tek Xpress x120 Operating Manual

Rapid tissue processor
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  • Page 1 x 120 Tissue-Tek Xpress ® ® Rapid Tissue Processor...
  • Page 4 © 2012 Sakura Finetek USA, Inc. All Rights Reserved Printed in U.S.A. Manufactured for: Sakura Finetek U.S.A., Inc. Torrance, CA 90501 U.S.A. 0004389-03 Rev.C...

  • Page 5: Table Of Contents

    TABLE OF CONTENTS Section Page INTRODUCTION Intended Use ..............................1.1 General Description ............................1.1 Safety Precautions ............................1.2 Location of Major Systems and Components ....................1.6 Specifications ............................. 1.12 Safety Standards ............................1.13 INSTALLATION Turning Instrument Power On or OFF ......................2.1 General Information ............................
  • Page 6 Rapid Tissue Processor Applications Manual Overview ............................... A.1 Preparation for Processing ........................... A.1 Use of Tissue-Tek Xpress x120 Pre-Processing Solution or Pre-Processing Fixative ......... A.1 Use of Tissue-Tek Molecular Fixative ......................A.2 Embedding of Tissue-Tek Xpress x120 Processed Tissue ................A.4 Microtomy ..............................

  • Page 7: Introduction

    Station for removal of the basket. The instrument escaping the instrument employs scheduling software to efficiently process and transfer sample baskets. The Tissue-Tek Xpress x120 instrument has the follow- ing features: • Instrument can be configured for either Manual or Automatic start-up •...

  • Page 8: Safety Precautions

    INTRODUCTION Safety Precautions NOTES, CAUTIONS, WARNINGS, and other safety related labeling are provided throughout this manual to indicate levels of potential hazards as defined below: NOTE Indicates a reminder or other helpful information. CAUTION Indicates a potential hazard in which failure to follow instruction may result in ®...
  • Page 9 Cabinet Front (Figure 1-A) Light On – indicates “access permitted” to the Loading Station access door; the door can be opened. The Loading Station Access Door ..— provides access to Loading Indicator is “on” when there is no basket in the the Loading Station to allow loading of tissue specimens Loading Station and the Transferring Arm is stationary.
  • Page 10 INTRODUCTION Unloading Indicator .. Fume Control System Access Door ..— provides an indication of the .. — provides status of the Unloading Station access door: access to the fume control system for purposes of replacing the fume filter. Light Off –...
  • Page 11 Internal Light Button ..— Turns the light inside the Tissue-Tek Xpress x120 on or off (Figure 1-D). The light must be manually turned off. Shutting down the instru- ment will not turn the light off.

  • Page 12: Location Of Major Systems And Components

    Unloading Station .. • Transfer System .. Components • Fume Control System .. • The Tissue-Tek Xpress x120 instrument is comprised of Reagent Cabinet .. • the following major systems and components (Figure 1-E): Paraffin Oven .. • Control Panel ..
  • Page 13 40 tissue cassettes, liters. Filing and draining of the retorts is accomplished by into the Tissue-Tek Xpress x120. The Loading Station a reagent flow circuit operating under software control. retort holds a maximum of approximately 1.8 liters of The flow control circuit incorporates an interlock that reagent (Preprocessing Solution, product code 7115).
  • Page 14 The reagent cabinet also interfaces • The upper sensor is used to prevent reagent with the Tissue-Tek Xpress x120 fume control system overflow. The sensor is actuated at approximately 2.5 (see “Fume Control System” on page 1.11 for details).
  • Page 15 Vacuum Stations (Retorts #3 and #4) Vacuum pumps are used to pump reagent (melted paraffin) into the Vacuum Station retorts. The vacuum NOTE: Retort #3 and Retort #4 are identical. pump for Vacuum Retorts applies vacuum for 80 seconds, These Vacuum Stations (Retorts #3 and #4) ( .. and then is off for 10 seconds (no vacuum applied).
  • Page 16 Figure 1-J The oven compartments are not removable, but slide in and out of the Tissue-Tek Xpress x120 cabinet for purposes of filling, draining, and maintenance. A spigot facilitates the draining of the reagents (see “Retort #3 and #4 Paraffin Replacement”...
  • Page 17 Transfer System Fume Control System (Figure 1-M) (Figure 1-N) The Transfer System performs the automated process of transferring baskets from the Loading Station to the Microwave and Vacuum Stations, and ultimately to the Unloading Station. The Transfer System is comprised of a motor-driven transfer arm ..

  • Page 18: Specifications

    INTRODUCTION Specifications Control Panel (Figure 1-O) The Control Panel ..provides the operating controls Models Covered By This Manual: and indicators necessary to operate the instrument, monitor system operation, configure system settings, and Product view status and historical reports. Code Name/Description ®...

  • Page 19: Safety Standards

    For Model 7721 – Storage: Temperature Range:-10°C to +65°C Japanese Pharmaceutical Law Relative Humidity: 30% to 95%, non-condensing Complies with: Relative Atmospheric Pressure: 70 to 106 kPa (525 to Quality Management System- ISO 13485: 2003 795 mmHg) Electrical Safety JIS C 1010-1: 2005 Dimensions: IEC61010:...
  • Page 20 INTRODUCTION 1.14 Revised 10/02/2012...

  • Page 21: Installation

    Read this operating manual carefully before attempting to operate the Tissue-Tek Xpress x120 instrument. Follow all instructions carefully. CAUTION: The Tissue-Tek Xpress x120 is a precision instrument and must be handled accordingly. Rough handling or dropping will disturb or damage internal components.

  • Page 22: Set Reagent Bottle

    INSTALLATION Set Reagent Bottle • Verify both reagent containers are full. Ensure both reagent containers are in their proper posi- 1. Place the new reagent container in the reagent cabinet tions in the cabinet, and that locking levers for in the appropriate position. both containers are in the locked position.

  • Page 23: Unpacking And Installation

    If any items are visibly damaged, or are missing, call 1-800-725-8723 or 1-310- Loading Station Container w/ Lid 972-7800 (U.S. Customers only). When located outside the (Product Code 7108) U.S., contact the nearest Sakura instrument distributor. Revised 10/02/2012...
  • Page 24 INSTALLATION Revised 10/02/2012...

  • Page 25: Customization Of Settings

    This section provides the procedures to perform initial setup of the Tissue-Tek Xpress x120 system settings. Factory default settings for each parameter are listed where applicable. Perform the following setup procedures before routinely using the Tissue-Tek Xpress x120 system.

  • Page 26: Configuring System Settings

    CUSTOMIZATION OF SETTINGS SOFTWARE VERSION – displays infor- for the instrument (selected parameters are indi- mation related to instrument software and cated by a “gray” background). firmware versions and serial number (see “Viewing the Software Version” on page 4.14 for details). SYSTEM SETUP –...
  • Page 27 10 feet of instrument). • OUTPUT –To determine method to export vari- The factory default setting for SOUND VOLUME ous data that Tissue-Tek Xpress x120 system is MIDDLE. recorded. NOTE: Touch the TEST CLICK, TEST ALARM, or Touch the desired option field, either PRINT or TEST END OF PROCESS button to test the selected FILEOUTPUT.
  • Page 28 Passwords determine the features and functions avail- 4. When all desired system settings/parameters have able to Tissue-Tek Xpress x120 users. Operator pass- been changed, touch the SAVE & EXIT button to words limit access to routine operations of the system.
  • Page 29 To access the PASSWORDS function: NOTE: Use the backspace key to edit a currently entered name. 1. From the UTILITIES screen, touch PASSWORDS button. 2. Touch the NAME field, then use the keyboard to enter • The PASSWORDS screen displays (Figure 3-D), the desired user name.
  • Page 30 CUSTOMIZATION OF SETTINGS Managing the User Defined File • The EDIT USER DEFINED ITEM screen displays (Figure 3-G). The USER DEFINED FILE function allows for creating “identifiers” that can be assigned to a basket or Paraform magazines on loading. The “identifiers” can then be used as sorting criteria for the run History File and Statistics.
  • Page 31 (ON) and automatic shutdown (OFF) time at which the instrument should be ready to begin times for the Tissue-Tek Xpress x120 for days on which processing) or “off” time (the time at which the instru- the system will normally be used (see “Setting Days Off”...
  • Page 32 CUSTOMIZATION OF SETTINGS To set A “Not In Use” day: To use the “days off” calendar: NOTE: “Not In Use” days should be set only for days on 1. Use the PREVIOUS and NEXT buttons at the top of which the instrument will not normally be operated. the screen to scroll the calendar to the desired month.

  • Page 33: Operating Instructions

    Section 4 OPERATING INSTRUCTIONS Initial Setup and Vacuum retorts, and Unloading Station, and provides tabular data related to the status of bas- Ensure that initial setup procedures have been per- kets currently being processed (see “Process formed in accordance with the requirements of Section 3, Monitoring”...

  • Page 34: Preparation For Processing

    2. Turn the handle clockwise until it is aligned with the Preparation for Processing recesses in the basket, then pull the handle free from the basket. The Tissue-Tek Xpress x120 instrument supports the use of standard cylindrical baskets, or Paraform maga- zines (for use in conjunction with the Tissue-Tek ®...

  • Page 35: System Start Up

    Manual Start Detailed instructions for preparing tissue samples prior to processing using the Tissue-Tek Xpress x120 instru- When the instrument is configured for Manual start, the ment are provided in Appendix A. instrument must be started manually.
  • Page 36 OPERATING INSTRUCTIONS System Warm Up (Figure 4-E) right-hand corner of the screen, and the name of the user is shown at the top of the screen. The system will not allow logging on to the instrument until the current user has logged off.

  • Page 37: System Operation

    Logging Off the System the ability to set the onboard preprocessing time between 0-60 minutes. Once a preprocessing NOTE: After loading a basket and initiating a processing cycle has been initiated, the preprocessing oper- cycle, it is recommended to log off the instrument if ation is performed automatically, under the con- individual passwords have been assigned to users.
  • Page 38 OPERATING INSTRUCTIONS Setting Onboard Preprocessing Loading Tissue Specimens and Times Initiating a Processing Cycle If this optional online preprocessing step is selected After the WARMING-UP SYSTEM cycle has completed, during setup, processing times will be altered accordingly. the Control Panel displays the PROCESS MONITOR A basket cannot move to the next retort until the set screen (see “Process Monitor Screen”...
  • Page 39 4. Place the basket or magazine set directly into the Loading Station retort (Figure 4-J). Close the Loading Station Access Door. Figure 4-L NOTE: If needed, use the backspace key to correct Figure 4-J errors when entering the number of cassettes. Touch the CAUTION: The Loading Station retort has slots ( ..
  • Page 40 OPERATING INSTRUCTIONS NOTE: Overuse of Pre-Processing Solution could lead to set preprocessing time and/or the availability of Retort #1. tissue artifacts on the stained slide. NOTE: If not processing additional baskets or magazine Process Monitoring sets at this time, logging off the instrument is recommend- ed (see “Logging Off the System”...
  • Page 41 • END TIME – shows the time at which the basket or magazine set is scheduled to be transferred into the Unloading Station, completing pro- Retort Icon (Ready) – indicates the associated cessing. retort is ready to accept a basket or magazine set. •...
  • Page 42 OPERATING INSTRUCTIONS NOTE: If REAGENT CONTROL is set to RUN, the 3. Remove the basket or magazine set from the number “40” will display in this field. Unloading Station and place the basket or magazine set on the transport tray for transportation to the par- Unloading Processed Specimens affin embedding system (Figure 4-P).
  • Page 43 NOTE: The heaters for Retorts #3 and #4, the paraf- fin oven, and all associated plumbing remain on to keep the paraffin in a melted state. Computer (Controller) • The computer switches to “sleep mode” after completing all reagent drainage. •...

  • Page 44: Special Conditions And Considerations

    OPERATING INSTRUCTIONS Special Conditions and 4. After the shutdown process has completed, replace all reagents (see “Pre-Processing Solution Replace- Considerations ment” on page 7.3 for details). On board Pre-Processing Solution must be replaced Understanding the Process on condition (after a specified number of hours) to ensure Scheduler proper preprocessing.

  • Page 45: Viewing Reports And Statistics

    Performing an Emergency Stop UTILITIES key to display the UTILITIES menu (Figure 4-S). The Process Monitor screen includes an emergency STOP button to stop the motion of the Transfer Arm and all processing actions in case of emergency. To perform an emergency stop: 1.
  • Page 46 OPERATING INSTRUCTIONS Viewing the Software Version The SOFTWARE VERSION screen displays infor- mation related to instrument software and firmware versions, and the instrument’s serial number. To view the software version: 1. From the UTILITIES screen, touch the SOFTWARE VERSION button. •...
  • Page 47 • Sort the RUN HISTORY screen chronologically by DATE, or alphabetically by OPERATOR, or LOCATION (User Defined File). To re-sort the RUN HISTORY screen, touch the column head chosen to use as a sort key. • Touch the EXIT button to return to the UTILITIES screen.
  • Page 48 OPERATING INSTRUCTIONS 2. Select the period for which to view statistics, either DAILY (for the prior seven days), WEEKLY (for the prior 7 weeks), or MONTHLY (for the prior 24 months). • The screen refreshes to display the selected DAILY STATISTICS (Figure 4-W), WEEKLY STATISTICS (Figure 4-X)
  • Page 49 SHOWN ON REPORT ITEM DEFINITION DAILY WEEKLY MONTHLY PERIOD The calendar date and day or week for which statistics are displayed. MONTH The month and year for which statistics are displayed. OPERATOR The operator with whom the statistics are associated. (The OPERATOR field is shown only when viewing statistics by operator.) User Defined File (“LOCATION”...
  • Page 50 OPERATING INSTRUCTIONS 3. If necessary, use the PERIOD (for DAILY or WEEKLY statistics) or MONTH (for MONTHLY statistics) drop- DATE & TIME The date and time at which the associated down menu to select the desired report period. error was logged. The identification number for the run that was 4.

  • Page 51: Recovery Process

    In this case, please contact the Sakura Technical Support Department at 800-725- 8723, option 2, (U.S. customers only), or the nearest authorized Sakura instrument distributor or representative.

  • Page 52: Starting A Processing Run In Retort 3 Or

    Retort 1 without compromising specimen integrity. removed from the system, and one of two op- NOTE: If the Tissue-Tek Xpress x120 will be down for longer tions, GO TO STANDBY or SHUTDOWN can be than 1 hour, place the tissue specimens in Molecular selected.
  • Page 53 Shows the time remaining until the TIME TO READY reagents for Retorts 3 and 4 are completely melted. If the instrument was shut down following an abort, 4:00 hours will be displayed (this is the amount of time required to completely melt the reagents).
  • Page 54 RECOVERY PROCESS Revised 10/02/2012...

  • Page 55: Consumables And Accessories

    Section 6 CONSUMABLES AND ACCESSORIES Consumable Materials Standard Accessory Items Product Product Code Description Code Description 1550 Paraffin Scraper 4295 Neutra-Pads (100/pk) 4785 Trimming Knife Blades, 130mm 4803 Grossing Fork (1.5mm) (50/pk)* 4804 Grossing Fork (2.0mm) 4807 Grossing Fork (2.5mm) 4786 Trimming Knife Handle* 4814...

  • Page 56: Maintenance And Service Items

    Accessories O71-697-01 Sight Tube Cleaning Brush If located within the United States, contact the Order Management Department of Sakura Finetek U.S.A, Inc., or by calling toll-free: 1-800-725-8723. Alternately, or in countries other than the United States, contact the nearest O71-491-00 Spill Tray authorized Sakura distributor.

  • Page 57: Care Of The Instrument

    Section 7 CARE OF THE INSTRUMENT General Information On Condition Maintenance This section provides information on how to care for Basket / Magazine Cleaning ® ® and maintain the Tissue-Tek Xpress x120. The system must be maintained correctly to ensure proper operation. CAUTION: Using baskets or magazines with paraffin Read this section carefully before attempting to perform residue will cause clogging of the reagent transfer line...
  • Page 58 (water temperature setting – HOT 100°C (212°F)). 3. Start the dishwasher. If an automatic dishwasher is not available for use in the laboratory, Tissue-Tek Xpress x120 processing 4. The cycle time will depend on the setting selections baskets, magazines, cover, and handle may be cleaned available on the dishwasher.
  • Page 59 Overflow Bottle Cleaning 3. Discard accumulated paraffin in the overflow bottles in accordance with local regulations for biohazardous The overflow bottles associated with Retort #3 and #4 waste. serve as overflow receptacles in the event excess reagent 4. Clean any paraffin residue in overflow bottles using (paraffin) is pumped into the associated retort.
  • Page 60 CARE OF THE INSTRUMENT Reagent Replacement Reagent must be replaced periodically (after a speci- fied number of runs or cassettes have been processed, or in the event an insufficient amount of reagent is available for processing) to ensure proper processing of tissue specimens.
  • Page 61 NOTE: Solely shaking the reagent bottle will not thorough- To replace paraffin: ly mix the solution. The bottle must be inverted. 1. Perform a system shutdown. 6. Remove the upper caps from the new reagent 2. Open the Paraffin Oven Access Door (Figure 7-F). containers, and install the caps on the used reagent containers.
  • Page 62 CARE OF THE INSTRUMENT 5. Using the shipping materials retained after the paraffin in accordance with local regulations for previous paraffin replacement, line the cardboard biohazardous waste. shipping carton with the plastic bags. Place the lined 10. Turn the locking knob on the lid of the Retort #3 cartons under the drain port for the Retort #3 paraffin paraffin oven clockwise, then lift the lid to open (Figure oven (Figure 7-H).

  • Page 63: Daily Maintenance

    Unloading Station Paraffin NOTE: The user must be logged on to the system as an Administrator in order to clear the reagent counter (see Replacement NOTE: The user must be logged on to the system as an If desired, paraffin can be used in the Unloading Sta- Administrator in order to clear the reagent counter (see tion retort container to ensure tissues are immersed in “Logging On”...

  • Page 64: Weekly Maintenance

    Exterior Cleaning To clean the retort mesh filters: Keep the exterior of the Tissue-Tek Xpress x120 cabi- 1. Open the Retort Access Door (Figure 7-N). The Access net free of dust and clutter. Generally, wiping with a clean Door supports will retain the door in the open position.
  • Page 65 CAUTION: Exercise care to avoid damaging the mesh 2. Press in on the paraffin oven to unlatch, then slowly filter during cleaning. Tears in the mesh may allow slide the oven out of the cabinet. debris to enter the reagent containers or paraffin 3.
  • Page 66 CARE OF THE INSTRUMENT Retort Sight Tube Cleaning remove debris from the wall of the sight tube (Figure 7-T). Retort Sight Tubes should be cleaned weekly to ensure reagent in Retorts #1 and #2 is filled and maintained at the appropriate levels for proper processing of tissue speci- mens, and to guard against reagent overflow.

  • Page 67: Monthly Maintenance

    Preheating Chamber Sight Tube 4. After cleaning, turn the sight tube access valve for Retort #1 preheating chamber clockwise to close the Cleaning valve. The Preheating Chamber sight tubes should be 5. Repeat steps 2 through 4 for the Retort #2 sight tube. cleaned weekly to ensure reagent in Retorts #1 and #2 is 6.
  • Page 68 CARE OF THE INSTRUMENT 1. Open the Fume Control System Access Door (Figure 4. Slide the fume filter out of the instrument (Figure 7-Z). 7-W). Figure 7-Z Figure 7-W 5. Install a new fume filter in the instrument. Lower the 2.

  • Page 69: Routine Maintenance Check List

    ® ® Tissue-Tek Xpress x120 Tissue Processor Routine Maintenance Check List, Daily Proper periodic maintenance of the Tissue-Tek ® Xpress ® x120 Tissue Processor is essential in maintaining the system in operational condition. Recom- mended periodic maintenance procedures and frequencies of performance are listed below. Keeping a running log of all routine maintenance preformed MONTH:__________________ ®...
  • Page 70 ® ® Tissue-Tek Xpress x120 Tissue Processor Routine Maintenance Check List, Weekly MONTH:__________________ Reagent Change Week 1 Beginning Date___________ Tues Thurs Lot# Lot# Lot# Lot# Lot# Lot# Lot# Replace On Board Reagent Set Cassette Count Clean Microwave Retort Mesh Filter Clean Microwave Chamber Sight Tube Clean Paraffin Retort Clean Paraffin Retort Mesh Filter...

  • Page 71: Troubleshooting

    Sakura Finetek Technical Support Representative for information or assistance. CAUTION: If any of the Access Doors are open, the Transfer System would be disabled and the baskets will not be transferred.

  • Page 72: Monitored Errors

    Tissue-Tek Xpress x120 display. To cancel (turn off) the alarm, press the EXIT button on the warning message.

  • Page 73: Service And Replacement Parts

    Where to Call for Service O71-169-01 Drip Tray If located within the United States, contact the Cus- O71-355-00 Reagent Bottle Tray tomer Support Department of Sakura Finetek U.S.A., Inc. D9-01-0102 Retort Cleaning Pipette by calling toll free: O71-692-00 Retort Mesh Filter Removal Tool...

  • Page 74: Optional Accessory Items

    Service Manual Where to Order: In the United States, the above Replacement Acces- sory Items may be ordered directly from: Order Management Sakura Finetek U.S.A., Inc. 1750 W. 214th Street Torrance, CA 90501 1-800-725-8723 Or contact the nearest authorized Tissue-Tek distribu- tor or representative.

  • Page 75: Overview

    XPRESS ® X120 RAPID TISSUE PROCESSOR APPLICATIONS MANUAL Overview Preparation for Processing The Tissue-Tek Xpress x120 can be configured for Tissue Processing on the Tissue- operation using one of two immersion time settings for ® Xpress x120 Rapid Tissue tissue processing based on the thickness of the grossed ®...

  • Page 76: Use Of Tissue-Tek Molecular Fixative

    NOTE: All tissue must be immersed in Pre-Processing Solution). Solution for a minimum of 15 minutes prior to being processed by the Tissue-Tek Xpress x120. CAUTION: Tissue specimens are to be held in 10% NBF until they are ready to start the preprocessing step.
  • Page 77 Sterile water come in contact with Formalin. RNase-free conditions of • Autoclave paraffin embedded tissue will yield intact RNA comparable to fresh tissue. If the Tissue-Tek Xpress x120 has been • ® RNaseZap Wipes (may be purchased from Ambion, used for processing Formalin-fixed tissue, the Tissue-Tek Inc, TX, USA www.ambion.com)*...

  • Page 78: Embedding Of Tissue-Tek Xpress X120 Processed Tissue

    WARNING: WEAR PROTECTIVE PERSONAL Paraffin embedded Tissue-Tek Xpress x120 tissue can EQUIPMENT WHEN CLEANING THE TISSUE-TEK be sectioned on a Microtome. The tissue blocks usually do XPRESS X120 PRIOR TO PROCESSING TISSUES FOR not require soaking. The blocks can be faced and allowed RNA STUDIES.

  • Page 79: Microscopic Evaluation

    Moderate swelling of red blood cells may be noted. Artifact - A retraction artifact that may be observed It is recommended that Tissue-Tek Xpress x120 rea- between epithelial cells and the underlying connective gents be replaced after processing up to approximately...

  • Page 80: Do's And Don'ts

    DO run RNA tissue after the processor is cleaned and solutions are all changed. • DO NOT process tissue with O.C.T. compound (used for Cryotomy) residue in the Tissue-Tek Xpress x120. • DO remove all O.C.T. compound (used for Cryotomy) from specimens by rinsing with water prior to placing in the Tissue-Tek Xpress x120.

  • Page 81: Tissue-Tek Xpress X120 Processing Flow Chart For Standard Program

    Tissue-Tek Xpress x120 Processing Flow Chart for Standard Program Tissues in cassettes sectioned at final thickness of 2 mm should be immersed for 1 hour in 10% NBF. Alternative step to address specific Immersion in PPS for 30 minutes. (offline) Pathologists’...
  • Page 82 Revised 10/02/2012...

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