Thermo Scientific Dionex IonPac CS14 Guard Product Manual page 5

IonPac CS14 Manual
SECTION 6 - TROUBLESHOOTING GUIDE ............................................................................... 42
6.1 High Back Pressure ............................................................................................................................................... 43
6.1.1 Finding the Source of High System Pressure ............................................................................................................ 43
6.1.2 Replacing Column Bed Support Assemblies ............................................................................................................. 44
6.2 Preparation of Eluents ............................................................................................................................................ 45
6.3 A Contaminated Guard or Analytical Column ........................................................................................................ 45
6.4 High Background or Noise ..................................................................................................................................... 45
6.5 A Suppressor Not Suppressing Properly ............................................................................................................... 46
6.6 Poor Peak Resolution ............................................................................................................................................. 47
6.6.1 Loss of Peak Efficiency throughout the Chromatogram ............................................................................................ 47
6.6.2 Loss of Resolution Throughout the Chromatogram Due to Shortened Retention Times ......................................... 47
6.6.3 Loss of Early Eluting Peak Resolution ...................................................................................................................... 48
6.7 Spurious Peaks ....................................................................................................................................................... 48
APPENDIX A - QUALITY ASSURANCE REPORT ....................................................................... 49
APPENDIX B - COLUMN CARE .................................................................................................. 52
Recommended Operating Pressures ................................................................................................................................ 52
Column Start-Up ................................................................................................................................................................ 52
Column Storage ................................................................................................................................................................. 52
Column Conditioning ......................................................................................................................................................... 52
Column Cleanup ................................................................................................................................................................ 52
Column Cleanup Procedure for Polyvalent Cations and Acid-Soluble Contaminants or Transition Metals ........................ 53
Hydrophobic Cations and Organic Contaminants ............................................................................................................... 53
Doc. No. 034848-10 Page 4 of 54