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B. Acquisition
1. Install your sample. To collect data uncheck the "Setup" box on the Acquisition Control
bar and click "Acquire". You can stop the collection by clicking "Abord" and the save
the current data by clicking "Save".
2. If you need to adjust the instrument settings as your cells are running through the
machine, check the "Setup" box on the Acquisition Control bar. Until that box is
unchecked, CellQuest will not collect any data.
3. You will hear a beep from the computer once the event count reaches the desired number
(usually 10-20,000 events). You can set this value under Acquire -> Acquisition and
Storage. This value will be saved with your template.
4. Repeat the procedure for the next sample.
C. After acquisition
1. Check sheath fluid level before starting the flushing procedure – it will be used during the
wash period.
2. Run the tube with 10% bleach for 3 minutes.
3. Repeat the same procedure with water to flush out the bleach.
4. Turn the fluid control to "Standby" and leave the nozzle in ~1ml (1cm) of water.
5. Vent the pressure by flipping the vent switch downwards.
Empty the waste tank and fill the sheath fluid reservoir if necessary (up to the red line).
6.
When completely finished, turn off the FACScan power if no other users are signed up
after you and then turn off the computer.
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