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Summary of Contents for BD FACSort
- Page 1 BD FACSort System After completing this module, you will be able to: • Describe the BD FACSort™ system and how it works. • Perform instrument startup and shutdown procedures. • Describe instrument maintenance procedures. BD FACSort System 337452 Rev. A...
- Page 2 BD FACSort System The BD FACSort™ system is an automated flow cytometer and cell sorter. It analyzes cells as they pass through a focused laser beam one at a time in a moving fluid stream. A BD FACSort flow cytometer can be used for routine research applications, immunophenotyping, and DNA cell-cycle analysis.
- Page 3 In this mode, back pressure is maintained to flush the sample injection tube. The system returns to RUN mode when you reinstall the sample tube. The pinch valve is opened and the laser returns to full power. BD FACSort System 337452 Rev. A...
- Page 4 This 4-liter container, located on the left, holds enough sheath fluid for approximately 3 hours of run time. It is equipped with a low-level fluid detector which indicates via software a near-empty condition. A metal bracket fits over the reservoir to secure the tank. BD FACSort System 337452 Rev. A...
- Page 5 The SIP is the area where the sample tube is installed. It consists of the sample injection tube through which the sample travels to the flow cell, the droplet containment system, and the tube support arm (Figure 3-2 on page 6). BD FACSort System 337452 Rev. A...
- Page 6 Its main function is to activate the droplet containment vacuum. The vacuum is activated when the arm is in the right or left positions and off when the arm is centered. BD FACSort System 337452 Rev. A...
- Page 7 flushing these tubings. Sort Line Purge Button The purge button located in the collection station automatically rinses the sort line when pressed. BD FACSort System 337452 Rev. A...
- Page 8 Turn on the computer. Choose your user ID and enter the password. Filling the Sheath Tank Recommended Sheath Fluid • BD FACSFlow™ sheath fluid (BD Biosciences) for nonsorting applications. • Phosphate-buffered saline (PBS) (Dulbecco’s Ca and Mg -free) for sorting applications.
- Page 9 Flip the vent valve toggle switch to pressurize the tank. Check the sheath tank to make sure it is properly pressurized. A properly pressurized sheath tank will not be able to move around under the bracket. BD FACSort System 337452 Rev. A...
- Page 10 The pressurized sheath fluid will force bubbles and sheath fluid out of the tubing. NOTICE Allow the laser to warm up for 5 minutes after turning on the instrument, before running samples. BD FACSort System 337452 Rev. A...
- Page 11 SIP with the support arm to the side (vacuum is on), and let it run for 1 minute. Use BD FACS clean solution or a 1:10 dilution of bleach in DI water as the bleach solution. BD FACS clean solution is a bleach-based cleaning agent for daily use in cytometer maintenance.
- Page 12 • Sample O-ring replacement The following section describes the monthly cleaning procedure. Other procedures can be found in the BD FACSort User’s Guide. Monthly Cleaning Perform overall system fluidics cleaning at least once a month or more frequently if you are running high volumes of samples or dyes such as propidium iodide (PI), acridine orange (AO), or thiazole orange (TO).
- Page 13 filter. Install a spare tank with 1 to 2 L of a bleach solution. Use BD FACS clean solution or a 1:10 dilution of bleach in DI water as the bleach solution. Press the HI sample flow rate button, then install a tube containing 3 mL of bleach solution on the SIP.
- Page 14 fluidic system until you use the instrument again. Contrad™ 70 is an excellent solution for removing clogs and wetting the fluidics. A 2% solution of Contrad 70 may be substituted for 10% bleach if necessary. BD FACSort System 337452 Rev. A...
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