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BD FACSAria II Quick Reference Guide
Use this quick reference guide once you are familiar with the procedures in
the BD FACSAria II User's Guide.
Starting Up the Cytometer
Follow these steps to start up your BD FACSAria™ II cytometer. For more
details, refer to Chapter 4 in the user's guide.
1
Start up the computer.
2
Turn on the cytometer main power and the lasers. Wait 30 minutes for
the lasers to warm up.
3
Start BD FACSDiva™ software and log in with your user
name and password.
4
Verify fluidics levels in the Cytometer window. Replenish
fluids or empty the waste, if needed.
5
Select Cytometer > Fluidics Startup.
6
Follow the instructions in the Fluidics Startup wizard to complete the
process.
Verify that the air and fluid lines are connected to the sheath tank, then
click Done.
main power
sheath waste
DI
bleach
Tip
If you are using temperature
control, start heating or cooling the
sample injection chamber or the
recirculating water bath while the
lasers are warming up.
ethanol
1
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Summary of Contents for BD FACSAria II
- Page 1 Use this quick reference guide once you are familiar with the procedures in the BD FACSAria II User’s Guide. Starting Up the Cytometer Follow these steps to start up your BD FACSAria™ II cytometer. For more details, refer to Chapter 4 in the user’s guide. Start up the computer.
- Page 2 Caution: Do not run fluidics startup with the air and fluid lines connected to the ethanol shutdown tank. This can cause damage to the system. Always verify the lines are connected to the sheath tank. Start the stream. Click the Sorting button ( ) on the Workspace toolbar to display the Breakoff and Side Stream windows.
- Page 3 Refer to Data Collection in Chapter 4 in the user’s guide. Install the required collection device and set up the side streams. Use BD FACS™ Accudrop to calculate the drop delay. Run the sample to be sorted. Use gating tools and subsetting methods to define the population(s) of interest.
- Page 4 If this is part of a daily shutdown, perform these steps. • Turn off the cytometer main power. • Exit BD FACSDiva software and shut down the computer. For instructions on running a complete fluidics shutdown, refer to the Fluidics Shutdown section in the BD FACSAria II User’s Guide.
- Page 5 Troubleshooting the Breakoff Normal stream Nozzle inserted Nozzle inserted Partial clog Wet or dirty Attenuation on image improperly improperly or strobe lens at the wrong orifice off pressure center Remove the Remove the Remove the Clean the lens Turn off nozzle and nozzle and nozzle, clean it,...
- Page 6 Toll Free 0120-8555-90 Toll Free 01-800-236-2543 help.biosciences@europe.bd.com Tel 81-24-593-5405 Tel (52) 55 5999 8296 Fax 81-24-593-5761 Fax (52) 55 5999 8288 BD, BD Logo and all other trademarks are property of Becton, Dickinson and Company. © 2007 BD 643246 Rev A...
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