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Thermo Scientific
Recommended Maximum Watts/Hour Limit
To prevent damage to the cassette or control unit, custom methods are limited to
25 watts/hour (when modifying pre-programmed methods and/or creating a new method). Any
voltage or amperage adjustments that result in exceeding the maximum 25 watts/hour will bring
up an alert triangle
on the Up arrow key. Continuing past this value will eventually result in
a Watt-Hour Limit warning window.
If the Watt-Hour Limit warning window appears, select the Okay button to return to the
previous screen and decrease appropriate number to return to a watt-hour value less than
25 watts/hour.
1. Pierce 1-Step Transfer Buffer is intended to rapidly transfer protein from gel to membrane
using a high transfer current (based on transfer area) and a short time (5-10 minutes).
a. Do not exceed 12-minute transfer times with rapid transfer protocols.
b. Do not exceed 22-23mA/cm
2. Conventional transfer buffers such as Towbin buffer (25mM Tris, 192mM glycine, 20%
methanol) are low ionic strength buffers and transfer time ranges from 45-60 minutes.
Current normally spikes and then quickly drops to a very low level, while voltage reaches
its maximum (25 volts).
(current/surface area) with rapid transfer protocols.
2
/ Thermo Scientific Pierce G2 Fast Blotter
Custom Methods
Section 4
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