Concentration 1/Diafiltration 1/Diafiltration 2/Concentration 2 Mode - REPLIGEN Spectrum KR2i User Manual And Operating Instructions

KR2i / KMPi TFF Systems
8.2.4

Concentration 1/Diafiltration 1/Diafiltration 2/Concentration 2 Mode

C/D/D/C Mode may be used to wash the solution with two different buffers.
Diafiltration Buffer Reservoir 1
Diafiltration Pump
Feed Scale
(Auxiliary Pump 1)
NOTE: In C/D/D/C Mode Auxiliary Pump 2 functions as Diafiltration Pump 2. For this functionality
test, prepare two buffer reservoirs that both lead into the Feed Reservoir, and have one line go
through Auxiliary Pump 1 and the other line go through the Auxiliary Pump 2.
Conc Factor 1
Diaf Volumes 1
Diaf Volumes 2
Conc Factor 2
44 400-12355-000 Rev. 03
Module
Feed Reservoir
KMPi Pump
Diafiltration Pump 2
(Auxiliary Pump 2)
Starting Weight
10X
Feed Wt After C1
7
8
20X Feed Wt After C2
System Functionality
Diafiltration Buffer Reservoir 1
Permeate Reservoir
Permeate Scale
20 kg
Perm Wt After C1
2 kg
Diaf Wt 1
Perm Wt After D1
Diaf Wt 2
Perm Wt After D2
1 kg
Feed Scale Permeate Scale
18 kg
14 kg
32 kg
16 kg
48 kg