Appendix B Tubing - GE HEALTHCARE AKTAprime plus Operating Instructions Manual

Compact liquid chromatography system designed for one-step purification of proteins at laboratory scale
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Appendix B
Tubing
Names in the Label column in
connection diagram, see
Table B.1: Tubing specifications for ÄKTAprime plus
Use
Inlet A11
Inlet A1
Inlet A2
Switch valve - Pump A3
Pump - Pressure monitor
Pressure monitor - Mixer
Mixer - Valve
Valve - UV (Column)
UV - Cond
Cond - Flow restrictor
Flow restrictor – Frac. coll.
Frac. tubing
Waste
ÄKTAprime plus Operating Instructions 28-9597-89 AC
Table B.1
Appendix A Connection diagram - Liquid flow path, on page
Label
Material
A1
FEP
A
FEP
B
FEP
AB
FEP
G1
PEEK
G2
PEEK
G3
PEEK
G4
PEEK
G5
PEEK
Union,
PEEK
1/16" male /
1/16" male
G6
PEEK
F1
PEEK
W1, W2, W3
PEEK
refer to tubing labels in the liquid flow path
Length
I.D.
(mm)
(mm)
1250
2.9
170
2.9
1250
2.9
510
1.6
150
0.75
120
0.75
300
0.75
650
0.75
250
0.75
38
0.50
550
0.75
410
0.75
1000
1.0
B Tubing
64.
Volume
(μl)
3
8.2 × 10
3
1.1 × 10
3
8.2 × 10
3
1.0 × 10
66
53
133
287
110
7
243
181
785
65

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