Pressure Curve Problems - GE HEALTHCARE AKTAprime plus Operating Instructions Manual

Error symptom
pH values vary with
varied back pressure
6.4

Pressure curve problems

Error symptom
Erratic flow, noisy
baseline signal, irregu-
lar pressure trace
ÄKTAprime plus Operating Instructions 28-9597-89 AC
Possible cause
Problem with the
electrode
Possible cause
Air bubbles passing
through or trapped in
the pump
Inlet or outlet check
valves not functioning
correctly
Piston seal leaking
Blockage or part
blockage of flow path
6 Troubleshooting
6.3 pH curve problems
Corrective action
Replace the pH electrode.
Corrective action
Check all connections for leaks.
Check that there is sufficient eluent
present in the reservoirs.
Use degassed solutions.
Purge the pump.
Follow the instructions in
ÄKTAprime plus User Manual.
Clean the valves according to
Pump P-920 User Manual.
Clean the valves according to
ÄKTAprime plus User Manual.
Replace the piston seal according
to the instructions in .ÄKTAprime
plus User Manual.
Flush through to clear blockage.
If necessary, replace tubing.
Check inlet tubing filter. It can be-
come clogged if unfiltered buffers
or samples are applied. See instruc-
tions for flushing through at the
end of the run in ÄKTAprime plus
User Manual.
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