GE HEALTHCARE Biacore T100 Manuals

Manuals and User Guides for GE HEALTHCARE Biacore T100. We have 1 GE HEALTHCARE Biacore T100 manual available for free PDF download: Handbook

GE HEALTHCARE Biacore T100 Handbook (239 pages)

system for analysis of biomolecular interactions

Brand: GE HEALTHCARE | Category: Laboratory Equipment | Size: 4 MB

System Overview
9
Support for Use in Regulated Environments
10
Operational Modes
15
User Interface
16
On-Line Help
17
Edit Menu
19
Run Menu
21
Right-Click Menus
22
File Storage
23
Preparing for a Manual Run
25
Starting a Manual Run
26
Controlling a Manual Run
27
Ending a Manual Run
29
Wizard Templates
31
Running Wizards
32
Assay Setup
34
Injection Parameters
35
Sample and Control Sample Tables
36
System Preparations
37
Rack Positions
39
Prepare Run Protocol
43
Surface Preparation Wizards
44
Immobilization
46
Assay Development Wizards
52
Buffer Scouting
55
Surface Performance
58
Assay Wizards
59
Concentration Analysis
62
Kinetics/Affinity
66
Thermodynamics
70
Control Experiment Wizards
72
Mass Transfer Control
73
Evaluation of Control Experiments
74
Opening Methods
77
Method Structure
78
Method Overview
79
General Settings
80
Assay Steps
81
Base Settings
82
Number of Replicates
84
Assay Step Preparations
85
Commands
86
Variables
92
Report Points
95
Variable Settings
96
Verification
97
Variables
98
Cycle Run List
99
Rack Positions
100
Kinetics/Affinity
101
Other Requirements
102
User Interface
105
The Evaluation Explorer
106
Common Display Functions
107
Predefined Evaluation Items
109
Custom Report Points
110
Adding Report Points
111
Editing and Deleting Report Points
112
Keywords
113
Solvent Correction
115
When Solvent Correction Should be Used
116
Applying Solvent Correction
117
Evaluation Methods
119
Applying Evaluation Methods
120
Sensorgram Items
121
Selecting Sensorgrams for Display
122
Sensorgram Adjustment
123
Markers
124
Plot Items
125
Selector Functions
126
Table Functions
127
Sorting the Plot
128
Adjusting Plots for Controls
130
Ranking
132
Bar Chart Items
133
Display Options
134
Requirements for Concentration Evaluation
139
Evaluating Calibrated Concentration Analyses
140
Control Samples
142
Samples
143
Custom Models for Calibration Curves
144
Evaluating Calibration-Free Measurements
145
Selecting Samples
146
Removing Disturbed Data
149
Interpreting the Results
150
Fitting Model
151
Requirements for Kinetics and Affinity Evaluation
154
Evaluating Kinetics and Affinity in Single Mode
155
Multiple Ligand Densities
162
Batch Mode Evaluation
163
Quality Assessment for Kinetics Evaluation
164
Statistical Parameters
168
Components of the Fit
170
Quality Assessment for Affinity Evaluation
172
Summarizing Kinetics and Affinity Results
173
On-Off Rate Maps
176
Curve Fitting Principles
177
Local and Global Parameters
178
Predefined Models
179
Kinetics – 1:1 Binding
180
Kinetics – Bivalent Analyte
181
Kinetics – Heterogeneous Analyte
182
Kinetics – Heterogeneous Ligand
184
Kinetics – Two State Reaction
186
Affinity – Steady State 1:1
188
Interaction Models for Kinetics
189
Equation Models for Kinetics
194
Models for Steady State Affinity
195
Background
197
Transition State Thermodynamics
198
Performing Thermodynamic Analysis
199
Conventions and Background
203
Requirements for Affinity in Solution
204
Evaluation of Affinity in Solution
205
A.1 Exporting Data
209
A.2 Importing Data
210
A.2.2 Evaluation Software
213
B.1 Affinity in Solution
215
B.2 Calibration-Free Concentration Analysis
216
B.2.3 Variable Settings
217
B.2.4 Setup Run
218
B.3 GST Kinetics
219
B.4 Inject and Recover
221
B.5 Kinetics Heterogeneous Analyte
223
B.7 LMW Kinetics and LMW Screen
224
B.8 NTA Kinetics
226
B.9 Single-Cycle Kinetics
227
Index
229

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GE HEALTHCARE Biacore T100 Manuals

GE HEALTHCARE Biacore T100 Handbook (239 pages)

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