Operation; Operation Overview; Starting The Instrument - GE HEALTHCARE AKTAprime plus Operating Instructions Manual

Compact liquid chromatography system designed for one-step purification of proteins at laboratory scale
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4 Operation

4
Operation
About this chapter
This chapter provides instructions for the use of ÄKTAprime plus.
4.1

Operation overview

Workflow
The typical workflow in ÄKTAprime plus, after turning on the system, can be divided into
a number of steps.
Step
Action
1
Prepare the system for a run
2
Start a run using a method
3
During a run - view and change parame-
ters
4
Procedures after a run
5
Evaluate the results
Liquid flow path
See
the liquid flow path in ÄKTAprime plus.
4.2

Starting the instrument

If the system is not already turned on:
1 Turn on the system using the Power switch at the rear panel. The system now per-
2 First the system name and software version number are displayed. Several messages
3 All parameters are automatically set to factory default values during the self-test.
32
Appendix A Connection diagram - Liquid flow path, on page 64
forms a self-test.
are then shown during the self-test. If an error is detected during the self-test, an
error message is shown.
Section
Section 4.3 Preparations before start, on page 33
Section 4.4 Performing a run, on page 38
Viewing the run, on page 39
Section 4.5 Procedures after a run, on page 40
See PrimeView user documentation.
ÄKTAprime plus Operating Instructions 28-9597-89 AC
for an illustration of

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