Ph Curve Problems - GE HEALTHCARE AKTAprime plus Operating Instructions Manual

6 Troubleshooting
6.2 Conductivity curve problems
Error symptom
Incorrect or unstable
reading
6.3

pH curve problems

Error symptom
No response to pH
changes
Small response to pH
changes
52
Possible cause
Loose connection of
conductivity flow ca-
ble
Incorrect pump and
valves function
Incorrect temperature
compensation factor
Dirty or incorrectly
equilibrated column
Incorrect mixer func-
tion
Possible cause
Faulty electrode con-
nection
Damaged electrode
Incorrectly connected
pH monitor
Dirty pH electrode
ÄKTAprime plus Operating Instructions 28-9597-89 AC
Corrective action
Check that the conductivity flow
cell cable is connected properly.
Check that the pump and valves
operate correctly.
If temperature compensation is
being used, check that the temper-
ature sensor is calibrated, and that
the correct temperature compen-
sation factor is in use.
Check that the column is equilibrat-
ed. If necessary clean the column.
Check the operation of the mixer.
The mixer function is checked by
placing a stirrer bar on top of the
mixer housing. The stirrer bar
should rotate when the system is
in Run mode. The mixer function
can also be checked by running
the installation test.
Corrective action
Check that the electrode cable is
connected properly.
The electrode glass membrane
may be cracked. Replace the elec-
trode.
Check that the pH monitor is cor-
rectly connected according to the
ÄKTAprime plus User Manual.
Clean the pH electrode as detailed
in Monitor pH/C-900 User Manual.
If the problem remains, replace the
pH electrode.