Applications; Certified Spectramax; M5 E -Htrf Readers; Optics - Molecular Devices SpectraMax M3 User Manual

Description
8

Applications

Endpoint, kinetic, spectrum, and multi-point well-scanning applications
combining absorbance and fluorescence in 6-well to 384-well
microplates, as well as endpoint, kinetic, and spectrum applications in
absorbance and fluorescence using cuvettes, can be run with little to no
optimization.
The extreme flexibility and high sensitivity of the SpectraMax Multi-
Mode Microplate Readers make them appropriate for applications within
the fields of biochemistry, cell biology, immunology, molecular biology,
and microbiology.
Typical applications include ELISA, nucleic acid, protein, enzymatic type
homogeneous and heterogeneous assays, microbial growth, endotoxin
testing, and pipettor calibration.

Certified SpectraMax

The SpectraMax M5
the M5 but is certified for use with Cisbio Bioassays' HTRF
(Homogeneous Time-Resolved Fluorescence) technology. HTRF is a
proprietary time-resolved fluorescence technology that overcomes
many of the drawbacks of standard Fluorescence Resonance Energy
Transfer (FRET) techniques, such as the requirements to correct for
autofluorescence and the fluorescent contributions of unbound
fluorophores.

Optics

The use of two holographic diffraction grating monochromators allows
for individual optimization of wavelengths for both excitation and
emission in fluorescence readings. Mirrored optics focus the light into
the sample volume, and cutoff filters are used to reduce stray light and
minimize background interference. The light source is a high-powered
Xenon flash lamp. Sensitivity or read-speed can be optimized by
varying the number of lamp flashes per read.

Dynamic Range

The dynamic range of detection is from 10
Variations in measured fluorescence values are virtually eliminated by
internal compensation for detector sensitivity, photomultiplier tube
voltage and sensitivity, as well as excitation intensity. The photometric
range is 0–4 ODs with a resolution of 0.001 OD.
M5 -HTRF Readers
® e
reader has the same performance specifications as
e
to 10 molar fluorescein.
-6 -12
0112-0115 F