Molecular Devices SpectraMax M3 User Manual page 68

Multi-mode microplate readers
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Specifications
68
Table A-1 Technical Specifications (cont'd)
Stray light
Photometric stabilization
Photometric drift
Calibration
Optical alignment
Light source
Average lamp lifetime
Photodetectors
Endpoint baseline noise (cuvette)
Endpoint kinetic noise (cuvette)
FLUORESCENCE INTENSITY PERFORMANCE
Sensitivity
Sensitivity (cuvette)
Wavelength range
Wavelength selection
Bandwidth (excitation, emission)
Number of excitation/emission pairs
per plate
Dynamic range
System validation
Light source
Average lamp lifetime
Detector
0.05% at 230 nm
Instantaneous
None — continuous referencing of
monochromatic input
Automatic before first kinetic read and
before every endpoint reading
None required
Xenon flash lamp (50 Watts)
1 billion flashes
Silicon photodiode
± 0.003 OD @190, 405, 850 nm
± 0.003 OD @190, 405, 850 nm
0.2 mOD/min and
Top Read
< 5 pM FITC, 1 fmol/200 µL(96),
< 20 pM, 2 fmol/100 µL(384)
Bottom Read
< 20 pM FITC(96)
< 15 pM fluorescein
250–850 nm
Monochromators, tunable in 1-nm
increments
9 nm, 15 nm
4
10
in 96-well black plates: auto gain
6
circuitry
Self-calibrating with built-in
fluorescence calibrators
Xenon flash lamp (1 joule/flash)
2 years normal operation
Photomultiplier (R3896)
0.2 mOD/min
0112-0115 F

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