Molecular Devices SpectraMax M3 User Manual page 4

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Contents
4
Chapter 2 Principles of Operation . . . . . . . . . . . . . . . . . 19
Absorbance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Optical Density. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Transmittance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Water Constant or Cuvette Reference? . . . . . . . . . . . . . 22
Background Considerations . . . . . . . . . . . . . . . . . . . . . 22
and Interfering Substances . . . . . . . . . . . . . . . . . . . . . 23
Normalizing Absorbance Measurements. . . . . . . . . . . . . 24
Fluorescence . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Luminescence . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Functional Description . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Temperature Regulation . . . . . . . . . . . . . . . . . . . . . . . 29
Read Types . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Endpoint Read . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Kinetic Read. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Spectrum Read. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Well Scan Read . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Automix . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Computer Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Chapter 3 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . 33
Unpacking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
Setting up the Instrument . . . . . . . . . . . . . . . . . . . . . . . 35
Installing the Drawer Adapter . . . . . . . . . . . . . . . . . . . . 36
Removing the Drawer Adapter . . . . . . . . . . . . . . . . . . . . 37
0112-0115 F

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