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Waters Xevo MRT Overview And Maintenance Manual

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Xevo MRT Mass Spectrometer
Overview and Maintenance Guide
715008899
© 2024 Waters Corporation
Version 02

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Summary of Contents for Waters Xevo MRT

  • Page 1 Xevo MRT Mass Spectrometer Overview and Maintenance Guide 715008899 © 2024 Waters Corporation Version 02...

  • Page 2: General Information

    This document is believed to be complete and accurate at the time of publication. In no event shall Waters Corporation be liable for incidental or consequential damages in connection with, or arising from, its use. For the most recent revision of this document, consult the Waters website (www.waters.com).

  • Page 3: Customer Comments

    To report any errors that you encounter in this document or to suggest ideas for otherwise improving it, reach us at tech_comm@waters.com. Contacting Waters Contact Waters with technical questions regarding the use, transportation, removal, or disposal of any Waters product. You can reach us through the Internet, telephone, or conventional mail. Contact method Information www.waters.com The Waters website includes contact information for Waters locations worldwide.

  • Page 4: Additional Resources

    (MRT) technology to a compact benchtop platform. The Xevo MRT is for both research and routine applications but is not intended for use in diagnostic applications. The Xevo MRT is intended for use in a laboratory environment only. It is not intended for use as an IVD medical device.

  • Page 5: Analyzing Samples From A Complex Matrix

    from day to day and run to run. Data collected when QC samples are out of range might not be valid. Do not report these data until you are certain that the instrument performs satisfactorily. Analyzing samples from a complex matrix When analyzing samples from a complex matrix such as soil, tissue, serum/plasma, whole blood, and other sources, note that the matrix components can adversely affect LC/MS results, enhancing or suppressing ionization.

  • Page 6: Ism Classification: Ism Group 1 Class A

    Milford, MA 01757 Safety considerations Some reagents and samples used with Waters instruments and devices can pose chemical, biological, or radiological hazards (or any combination thereof). You must know the potentially hazardous effects of all substances you work with. Always follow good laboratory practices and consult your organization’s standard operating procedures as well as your local requirements for...

  • Page 7: Applicable Symbols

    Applicable symbols The following symbols can be present on the device, system, or packaging. Symbol Definition Manufacturer Date of manufacture Confirms that a manufactured product complies with all applicable European Community directives UK Conformity Assessed marking confirms that a manufactured product is in conformity with the applicable requirements for products sold within Great Britain Australia EMC compliant...

  • Page 8: Safety Hazard Symbol Notice

    Symbol Definition For compliance with Waste Electrical and Electronic Equipment legislation, contact Waters Corporation for the correct disposal and recycling instructions For indoor use only No pushing Do not connect to an LC system Indicates the maximum load you can place on...

  • Page 9: Considerations Specific To The Device

    Solvent leakage hazard The source exhaust system is designed to be robust and leak-tight. Waters recommends that you perform a hazard analysis, assuming a maximum leak into the laboratory atmosphere of 10% LC eluate.

  • Page 10: Bottle Placement Prohibition

    Warning: To avoid risk of explosion, use tetrahydrofuran containing an inhibitor. Peroxides in inhibitor-free THF can spontaneously explode when concentrated by evaporation. Warning: To avoid risk of explosion, do not use tetrahydrofuran that has been open and exposed to air for an extended length of time. Use fresh tetrahydrofuran whenever possible.

  • Page 11: High-Voltage Hazard

    High-temperature hazard Warning: To avoid burn injuries, avoid touching the listed components when operating or maintaining the instrument. Xevo MRT high-temperature hazard Source ion block assembly Desolvation heater and source enclosure Hazards associated with removing an instrument from service Warning:...

  • Page 12: Safe Disposal

    Do not dispose of the instrument or return it to Waters for repair until the authority responsible for approving its removal from the premises specifies the extent of decontamination required and the level of residual contamination permissible.

  • Page 13: Safety Advisories

    For details on regional arrangements, see EU WEEE Compliance. • Some Waters instruments use batteries, mercury-containing lamps, or other replaceable components during the life span of the instrument. Handle such materials in accordance with local laws governing their processing and safe disposal.

  • Page 14: Table Of Contents

    General information......................ii Audience and purpose..........................ii Copyright notice............................ii Trademarks.............................ii Customer comments..........................iii Contacting Waters..........................iii Additional resources........................iv Intended use of the Xevo MRT......................iv Calibrating..............................iv Quality control............................iv Analyzing samples from a complex matrix..................v EMC considerations..........................v FCC radiation emissions notice....................... v Controlled electromagnetic environment..................v Canada spectrum management emissions notice................
  • Page 15 2.4 Leaving the mass spectrometer ready for operation..............38 2.5 Monitoring the mass spectrometer LEDs..................38 2.6 Tuning and calibration information....................39 2.7 Flow rates for the Xevo MRT system..................... 39 2.8 Restarting the mass spectrometer....................40 2.9 Emergency shutdown of the mass spectrometer................40 November 19, 2024, 715008899 Ver.
  • Page 16 3 Configuring the LockSpray II source...............41 3.1 Configuring the LockSpray II source....................41 3.2 ESI and APCI modes........................41 3.2.1 ESI mode..........................42 3.2.2 APCI mode..........................42 3.2.3 Configuring for ESI/APCI modes..................43 3.2.4 Installing the probe adapter....................46 3.2.5 Installing the probe assembly....................49 3.2.6 Optimizing the probe position....................
  • Page 17 5.4 Preparing the instrument for working on the source...............84 5.4.1 Using waters_connect software to prepare the instrument for operations on or inside its source............................84 5.5 Removing and refitting the source enclosure................. 85 5.5.1 Removing the source enclosure from the instrument............85 5.5.2 Fitting the source enclosure to the instrument..............
  • Page 18 5.16 Removing and fitting the sampling cone assembly retaining blocks.......... 130 5.16.1 Removing the sampling cone assembly retaining blocks..........130 5.16.2 Fitting the sampling cone assembly retaining blocks............133 5.17 Replacing the ion block source heater..................135 5.18 Cleaning or replacing the corona pin..................138 5.19 Cleaning the StepWave ion guide assembly................
  • Page 19 A.1.1 Specific warnings........................191 A.2 Notices............................193 A.3 Bottles Prohibited symbol......................193 A.4 Required protection........................193 A.5 Warnings that apply to all Waters instruments and devices............194 A.6 Warnings that address the replacement of fuses.................198 A.7 Electrical symbols........................199 A.8 Handling symbols.........................200 B External connections....................202 B.1 Mass spectrometer external wiring and vacuum connections.............
  • Page 20 B.13 Connecting the camera for the NanoLockSpray source............222 B.13.1 Installing the camera driver software................223 C Materials of construction and compatible solvents..........224 C.1 Preventing contamination......................224 C.2 Items exposed to solvent......................224 C.3 Solvents used to prepare mobile phases..................225 November 19, 2024, 715008899 Ver.

  • Page 21: Waters Xevo Mrt Overview

    This chapter describes the instrument, including its controls, sources, and the fluidics system. 1.1 Waters Xevo MRT MS The Xevo MRT is an orthogonal acceleration, time-of-flight (Tof) mass spectrometer controlled by Waters software (waters_connect Software Platform). Either of the following high-performance, ZSpray, dual-orthogonal API sources is fitted as standard equipment: •...

  • Page 22: Waters Reagent Delivery System (Wrds)

    Figure 1–1: Xevo MRT ion optics overview Analyte sprayer Pusher LockMass sprayer Multi-Reflecting Tof (MRT) region StepWave XS ion Detector guide Quadrupole Gridless mirrors Collision cell Focusing region To pump or pumps Isolation valve 1.2 Waters Reagent Delivery System (WRDS) 1.2.1 Waters Reagent Delivery System overview...
  • Page 23 Requirement: Wear clean, chemical-resistant, powder-free gloves when performing this procedure. The Waters Reagent Delivery System (WRDS) controls how calibrant or lock mass reference solutions are delivered to the MS source. See item three in system image Figure 1–6: ACQUITY UPLC/ Xevo MRT system (Page 29).
  • Page 24 Note: A Harvard Pump 11 Elite syringe pump can be used for infusing calibrant and lockmass reference solutions if a Waters RDS is not available. When using a syringe pump, observe the following warnings: November 19, 2024, 715008899 Ver. 02...

  • Page 25: Wrds Valve Connections

    Warning: To avoid any risk of a static-like electric shock: • Switch to power save before touching the syringe. • Use only non-metallic fittings for the syringe connection. Warning: To avoid risk of syringe breakage while using a syringe pump: •...

  • Page 26: System Operation

    1.3 Waters Xevo MRT diverter valve The Xevo MRT MS has a built-in diverter valve on the front of the instrument used for LC and probe connections. For standard LockSpray applications, the diverter valve connections are as follows: November 19, 2024, 715008899 Ver.

  • Page 27: Standard- And Nano-Flow Fluid Path Connections Overview

    Always insert a blank plug into port 3 of the diverter valve. 1.4 Standard- and nano-flow fluid path connections overview The Xevo MRT MS can be configured for both standard- and nano-flow operation. Standard flow operation (LockSpray source) The LC flow is connected to the ESI or APCI probe via the diverter valve. See,...

  • Page 28: Acquity Uplc/ Xevo Mrt Ms Systems

    The standard WRDS cannot deliver solutions at the low flow rates required for nano-flow. 1.5 ACQUITY UPLC/ Xevo MRT MS systems The Waters Xevo MRT is compatible with ACQUITY UPLC systems. If you are not using an ACQUITY UPLC system, refer to the documentation relevant to your LC system.

  • Page 29: Waters Acquity Uplc/ Xevo Mrt Ms System

    For additional information, see the ACQUITY UPLC System Operator's Guide (71500082502) or Controlling Contamination in LC/MS Systems (715001307). You can find these documents on www.waters.com. Click Support > Support Documents and Downloads. 1.5.3 Waters ACQUITY UPLC/ Xevo MRT MS System Figure 1–6: ACQUITY UPLC/ Xevo MRT system Solvent bottle...

  • Page 30: Acquity Uplc M-Class System

    The ACQUITY UPLC M-Class system includes a binary solvent manager, an auxiliary solvent manager if present, a sample manager, a column heater, a sample organizer, detectors, and a specialized ACQUITY UPLC M-Class column. Waters informatics software controls the system. For further information, see the ACQUITY UPLC M-Class System Guide (715003588) or Controlling Contamination in LC/MS Systems (715001307).

  • Page 31: Lockspray Source And Ionization Modes

    Configuring the LockSpray II source (Page 41)). Figure 1–8: Xevo MRT MS fitted with LockSpray source LockSpray source 1.6.1 Electrospray ionization (ESI) In ESI, a strong electrical charge is applied to the eluent as it emerges from a nebulizer. The droplets that compose the resultant aerosol undergo a reduction in size (solvent evaporation).

  • Page 32: Apci Mode

    solvent continues to evaporate, the charge density increases until the droplet surfaces eject ions (ion evaporation). The ions can be singly or multiply charged. To operate the LockSpray source in ESI mode, you fit the source enclosure with an ESI probe. The standard ESI probe capillary accommodates flow rates of up to 2 mL/min, making it suitable for LC applications in the range of 100 µL/min to 2 mL/min.
  • Page 33 Setting up the camera (Page 76). Figure 1–10: Xevo MRT MS fitted with NanoLockSpray source NanoLockSpray source Options shown in the following table are available for the spraying capillary: Table 1–1: Spraying-capillary options...

  • Page 34: Software And Data System

    • Printing data 1.8.1 waters_connect software waters_connect is Waters’ groundbreaking informatics solution that draws on our strong legacy of excellence in software innovation. It brings a wealth of new features, quality improvements, and access to new applications to help you meet data integrity and compliance regulations while benefiting from ultimate flexibility and usability.

  • Page 35: Automation

    1.5 mL of accumulated leaked liquid in its surrounding reservoir. At the same time, the software displays an error message alerting you that a leak has developed. Consult the Waters ACQUITY UPLC Leak Sensor Maintenance Instructions (71500082506) for complete details.

  • Page 36: Preparing The Mass Spectrometer For Operation

    This chapter describes how to start and shut down the mass spectrometer. 2.1 Preparing to start the mass spectrometer The Waters Xevo MRT is compatible with several types of ACQUITY UPLC systems. See ACQUITY UPLC/ Xevo MRT MS systems (Page 28) for details of compatible ACQUITY UPLC systems.

  • Page 37: Starting The Mass Spectrometer

    6. Navigate to the system control panel within the software. 7. From the Console Navigation pane on the left-hand side of the window, in the System pane, click the instrument ( Xevo MRT). 8. Wait for the vacuum to reach the required level.

  • Page 38: Verifying The Instrument's State Of Readiness

    Requirement: Before acquiring data, ensure that the instrument is pumped down (evacuated) for at least three hours and in Operate for at least 30 minutes. 2.3 Verifying the instrument’s state of readiness When the instrument is in good operating condition, the Power and Status LEDs show steady green.

  • Page 39: Tuning And Calibration Information

    2.7 Flow rates for the Xevo MRT system The Xevo MRT system can run at high flow rates. To optimize desolvation and sensitivity, run the system at appropriate gas flows and desolvation temperatures. Table 2–3: Flow rate versus temperature and gas flow...

  • Page 40: Restarting The Mass Spectrometer

    2.8 Restarting the mass spectrometer Restart the mass spectrometer when any of these conditions apply: • The software fails to establish communication or loses communication with the mass spectrometer. • You perform a software upgrade. • The main power supply is disrupted. To restart the mass spectrometer: 1.

  • Page 41: Configuring The Lockspray Ii Source

    3 Configuring the LockSpray II source This section explains how to configure the LockSpray II source for the following ionization modes: • ESI (electrospray ionization) • APCI (atmospheric pressure ionization) 3.1 Configuring the LockSpray II source The following table summarizes how you configure the LockSpray II source for the various ionization modes.

  • Page 42: Esi Mode

    Figure 3–1: LockSpray II source enclosure Reference sprayer port Vernier screw for horizontal adjustment of the probe adapter Probe adjuster assembly 3.2.1 ESI mode To operate in ESI mode, you must fit the ESI probe adapter to the source enclosure and install a probe assembly.

  • Page 43: Configuring For Esi/Apci Modes

    Figure 3–2: APCI mode APCI probe Corona pin Sample cone Hot gas from the APCI probe passes between the sample cone and the corona pin, which is typically operated with a discharge current of 5 µA. Mobile phase molecules rapidly react with ions generated by the corona discharge to produce stable reagent ions.
  • Page 44 Figure 3–3: Probe adapter types ESI probe adapter APCI probe adapter APCI identification label APCI probe heater ESI probe tip ESI identification label November 19, 2024, 715008899 Ver. 02 Page 44...
  • Page 45 Mode Probe adapter Install corona pin APCI APCI For more information on using each mode, see the Xevo MRT system online Help. The following sections explain how to complete the following tasks: • Installing the probe adapter (Page 46) •...

  • Page 46: Installing The Probe Adapter

    3.2.4 Installing the probe adapter Figure 3–5: Probe adapter parts Probe adapter cap removed from probe adapter Probe adapter cap tether Locking ring Probe adapter identification label Probe adapter cap release buttons Required materials • Chemical-resistant, powder-free gloves To install the probe adapter: Warning: To avoid personal contamination with biologically hazardous or toxic compounds, wear clean, chemical-resistant, powder-free gloves when performing this procedure.
  • Page 47 Notice: The probe tip is easily damaged. Take care not to bend, crush, or distort the probe tip. Fit the protective cap to the probe tip if you remove the probe adapter from the source. 1. For ESI probe adapters, remove the protective cap, if fitted, from the probe tip. Figure 3–6: ESI probe protective cap ESI probe protective cap 2.
  • Page 48 Figure 3–8: Locating the ESI probe adapter Location hole for probe location dowel Probe adjuster assembly Figure 3–9: Locating the APCI probe adapter Location hole for probe location dowel Probe adjuster assembly 3. Rotate the probe adapter locking ring clockwise to secure the probe adapter in place. November 19, 2024, 715008899 Ver.

  • Page 49: Installing The Probe Assembly

    Figure 3–10: Probe adapter mounted on the source enclosure Probe adapter cap Probe adapter locking ring Probe adjuster assembly Source enclosure High-voltage connector ESI probe adapter cable (ESI probe adapter only) 4. For ESI probe adapters, connect the ESI probe adapter’s cable to the high-voltage connector.
  • Page 50 Recommendation: To connect the probe assembly directly to the fluidics valve, use the 500-mm ESI or APCI probe assembly. Figure 3–11: Probe assembly Capillary Probe adapter PEEK fitting Warning label Identification label showing part number Fitting spring retainer Probe inlet fitting Notes: The high-voltage warning label does not appear on all probe assemblies.
  • Page 51 Warning: To avoid electric shock, do not insert any item into the probe cap aperture when the probe cap is fitted to the instrument. Warning: To avoid harmless, static-like electric shock, ensure that the mass spectrometer is in Standby mode before you touch any of its external surfaces that are marked with this high-voltage warning symbol.
  • Page 52 Figure 3–13: Inserting the probe assembly Probe assembly capillary Probe adapter 3. Screw the probe adapter fitting into the probe adapter, finger-tight only, until you hear a click. Figure 3–14: Probe assembly fitted to the probe adapter Probe adapter fitting Tip: The probe adapter fitting varies in size depending on the probe assembly type.
  • Page 53 the correct probe assembly. For example, the UniSpray probe assembly will not fit the tool-free probe adapter. 4. Tilt the probe adapter cap so that the ball bearing is located in the recess at the bottom of the aperture, and then insert the probe assembly tubing through the aperture. Figure 3–15: Probe adapter cap Probe cap aperture from the underside Probe cap aperture from the top...
  • Page 54 Figure 3–16: Fitting the probe adapter cap Inlet fitting Probe adapter cap Probe adapter cap tether Probe adapter Probe assembly capillary 5. Slide the probe adapter cap along the probe assembly, over the probe adapter inlet fitting. 6. Push the probe adapter cap onto the probe adapter until it clicks. Tips: November 19, 2024, 715008899 Ver.
  • Page 55 • Do not squeeze the probe adapter cap release buttons when fitting the probe adapter cap. • Ensure that the probe adapter cap is correctly seated and that both release buttons engage with the probe adapter, producing a click. • If you cannot fit the probe adapter cap fully, ensure that you are installing the correct probe assembly type.
  • Page 56 Figure 3–18: Inlet fitting holder Probe assembly capillary Probe inlet fitting Inlet fitting holder Warning: To avoid risk of electric shock, connect the ESI probe capillary to the diverter valve or, if connected directly to an LC system, to a grounded liquid outlet.
  • Page 57 Figure 3–19: Connecting the probe inlet fitting Diverter valve Probe assembly capillary November 19, 2024, 715008899 Ver. 02 Page 57...

  • Page 58: Optimizing The Probe Position

    Vernier probe adjuster (for horizontal adjustment of the probe) 3.2.6 Optimizing the probe position Before you begin using the Xevo MRT for ESI or APCI, you must visually inspect the initial probe position. You can then optimize the probe position using the manual tuning controls for your...
  • Page 59 Figure 3–21: Probe mounted on the Xevo MRT source Probe assembly adjuster Vernier screw for horizontal adjustment of the probe adapter Note: Position 6 on the vernier screw gives the best balance of sensitivity and robustness for the majority of compounds with typical LC flow rates. Spraying directly onto the instrument cone has an adverse effect on robustness.

  • Page 60: Removing The Probe Adapter

    Software system Settings then click Operate in the upper, right- hand corner of the window. The API and collision gas flows start. 3. View the probe tip through the source window, and use the probe assembly adjuster to adjust the capillary so that it protrudes by approximately 0.5 mm from the end of the probe tip.
  • Page 61 • Switching between ESI and APCI modes • Replacing the ESI probe tip or gasket (see Replacing the ESI probe tip and gasket (Page 156)) • Installing the Low-flow ESI probe (see the Low-flow ESI Probe Operator's Guide) Tip: You can replace the probe assembly without removing the ESI probe tip. Note: You can remove the probe adapter with or without the probe assembly installed.

  • Page 62: Installing And Removing The Corona Pin

    The corona pins can become misshapen in transit, or when the pin is fitted or removed. To avoid this and ensure that the tip of the corona pin is fitted in the optimum operating position, Waters recommends that you use the supplied corona pin alignment tool when you fit the corona pin.
  • Page 63 Figure 3–22: Inside the source enclosure Rear shield Corona pin mounting contact blanking plug Sampling cone assembly Front shield Isolation valve 3. Wait six minutes for the isolation valve, the sampling cone handle, and the source shield to cool. Warning: To avoid burn injuries, use caution when working with the isolation valve or the sampling cone handle.
  • Page 64 Figure 3–23: Fitting the corona pin alignment tool Corona pin alignment tool Corona pin alignment point Handle Corona pin mounting contact blanking plug 8. Grasp the corona pin alignment tool handle, and use it to rotate the corona pin alignment tool 90 degrees, moving the handle downward from the horizontal to the vertical position.
  • Page 65 Figure 3–25: Fitting the corona pin Corona pin 10. Bend the corona pin so that the tip is positioned within the depression at the tip of the corona pin alignment tool. Figure 3–26: Fitting the corona pin Corona pin alignment tool Corona pin 11.
  • Page 66 Figure 3–27: Source enclosure Source window Vernier probe adjuster 3.2.8.2 Removing the corona pin from the source Required materials Chemical-resistant, powder-free gloves To remove the corona pin from the source: Warning: To avoid personal contamination with biologically hazardous, toxic, and corrosive materials, wear chemical-resistant, powder-free gloves and protective eyewear when performing this procedure.
  • Page 67 Warning: To avoid burn injuries, take great care while working with the source enclosure open. • Open the source enclosure and wait six minutes for the isolation valve and sampling cone handles to cool before touching them. • Open the source enclosure and wait at least 30 minutes for the ion block to cool before removing it.
  • Page 68 Sampling cone assembly Front shield Isolation valve 7. Close the source enclosure. November 19, 2024, 715008899 Ver. 02 Page 68...

  • Page 69: Configuring The Nanolockspray Source

    4 Configuring the NanoLockSpray source The NanoLockSpray electrospray ion source enables the optimized co-introduction of sample and lock mass compound directly into the ion source. At low flow rates, this feature provides authenticated, exact-mass measurement in both MS and MS/MS modes. 4.1 Overview of the NanoLockSpray source Warning: To avoid electrical shock, never operate the Nanoflow source without the...
  • Page 70 Shield holding screw Y-position adjuster Thumbscrew Sprayer-platform adjuster assembly Thumbscrew (on left-hand side of sprayer platform) X-position adjuster Sprayer cover Clear sprayer shield (fitted) Z-position adjuster Reference probe The NanoLockSpray source enclosure holds two NanoFlow sprayers positioned orthogonally with respect to one another. The sample flows through one sprayer and the lock mass reference solution through the other.

  • Page 71: Sample Sprayer

    Sample inlet Reference inlet Spray indexing permits acquisition of sample and LockSpray data in separate data channels, and the baffle design ensures negligible cross-talk between the two sprays. The LockSpray data are used to calculate a correction factor for the mass scale calibration, which is then applied to the sample data, providing exact mass information.

  • Page 72: Installing The Nanolockspray Source

    4.2 Installing the NanoLockSpray source Required materials Chemical-resistant, powder-free gloves Warning: To avoid personal contamination with biologically hazardous, toxic, and corrosive materials, wear chemical-resistant, powder-free gloves and protective eyewear when performing this procedure. Warning: To avoid static-like electric shock, ensure that the instrument is prepared for working on the source before starting this procedure.
  • Page 73 Figure 4–3: Removing the stage Stop screw Retaining screw 4. Using both hands, fit the NanoLockSpray source enclosure to the two supporting studs on the source adapter housing. 5. Close the source enclosure door. 6. Connect a 1/16-inch PTFE tube between the mass-flow controller output (mounted beneath the stage on the front of the NanoLockSpray source) and your sprayer.

  • Page 74: Selecting And Configuring The Nanolockspray Source

    Figure 4–4: Connecting the high-voltage cable High-voltage cable 10. Close the instrument’s source interface door. 4.3 Selecting and configuring the NanoLockSpray source The Universal NanoFlow sprayer is installed as standard equipment on the NanoLockSpray source. For installation and maintenance details, see the Universal NanoFlow Sprayer Installation and Maintenance Guide (71500110107).

  • Page 75: Deploying The Sprayer Platform Adjuster Assembly

    4.4 Deploying the sprayer platform adjuster assembly 4.4.1 Moving the sprayer platform out of the source To move the sprayer platform out of the source: 1. Confirm that the sprayer cover is installed and in place over the sprayer (see Overview of the NanoLockSpray source (Page 69)).

  • Page 76: Setting Up The Camera

    • If you observe an electrical discharge between the sprayer tip and baffle, move the tip farther from the baffle or reduce the capillary voltage. The capillary voltage must be high enough to maintain a good spray. • Small adjustments to the sprayer position can make large differences to the source sensitivity.

  • Page 77: Optional Glass Capillary Sprayer

    Figure 4–5: Camera Control view of sprayers and sample cone Sample cone Baffle Sprayer tip Sample sprayer 4.7 Optional glass capillary sprayer The glass-capillary sprayer is designed for use with metal-coated borosilicate glass capillaries. The glass capillaries allow extremely low flow rates (less than 100 nL/min). You only use the glass capillaries for one sample, and then must discard them.

  • Page 78: Fitting A Borosilicate Glass Capillary (Nanovial)

    4.7.1 Fitting a borosilicate glass capillary (nanovial) Required materials • Chemical-resistant, powder-free gloves • Needle-nose pliers • Borosilicate glass capillary • Fused silica syringe needle or GELoader tip • Fused silica tubing cutter To fit a borosilicate glass capillary (nanovial): Warning: To avoid lacerations, puncture injuries, and possible contamination with biologically hazardous and toxic materials, do not touch the sharp end of the capillary.
  • Page 79 Figure 4–6: Unscrewing the union from the sprayer assembly Capillary Union 6. Remove the existing capillary from the sprayer. 7. Carefully remove the new borosilicate glass capillary from its case by lifting it vertically while pressing on the foam with two fingers. Figure 4–7: Removing the borosilicate glass capillary Foam Capillary...
  • Page 80 Recommendation: When using a GELoader tip, break the glass capillary in half, scoring it with a fused silica cutter so that the GELoader can reach the capillary’s tip. 9. Thread the knurled nut and approximately 5 mm of conductive elastomer over the blunt end of the capillary.
  • Page 81 14. In the MS Tune window, ensure that the Capillary parameter on the ES+/- Source tab is set to 0 kV. 15. Carefully push the stage back into the NanoLockSpray source enclosure, using the stop and handle. November 19, 2024, 715008899 Ver. 02 Page 81...

  • Page 82: Maintenance Procedures

    5 Maintenance procedures This section provides the maintenance guidelines and procedures necessary to maintain the instrument's performance. Keep to a maintenance schedule and perform maintenance as required and described in this section. 5.1 Maintenance schedule The following table lists periodic maintenance schedules that ensure optimum instrument performance.

  • Page 83: Spare Parts

    Replacing the mass spectrometer air filters. spectrometer’s air filters (Page 167). 5.2 Spare parts To ensure that your system operates as designed, use only Waters Quality Parts. Visit https:// www.waters.com/nextgen/nl/en/c/promo/spare-parts.html for information about Waters Quality Parts, including how to order them.

  • Page 84: Preparing The Instrument For Working On The Source

    1. Navigate to the system control panel within the software (waters_connect Hub > System Console). 2. From the Console Navigation pane on the left-hand side of the window, in the System pane, click the instrument ( Xevo MRT). November 19, 2024, 715008899 Ver. 02 Page 84...

  • Page 85: Removing And Refitting The Source Enclosure

    3. To put the instrument into Standby, click Summary > Summary, and then click in the upper right-hand corner of the window. Tip: You can also put the instrument into Standby from the Manual Optimization pane. To do this, from the Console Navigation pane, click Optimization > Manual Optimization, and then click in the upper right-hand corner of the pane.
  • Page 86 Wait until the hot components have sufficiently cooled before you handle them. 2. If the probe assembly is fitted, disconnect the probe inlet fitting from the Xevo MRT diverter valve. 3. Remove the probe adapter from the source (see Removing the probe adapter (Page 60)).

  • Page 87: Fitting The Source Enclosure To The Instrument

    Figure 5–1: Source enclosure removed from instrument Supporting stud Source enclosure Cable storage positions 9. Store the cables neatly by plugging them into the cable-storage positions on the rear of the source enclosure. 5.5.2 Fitting the source enclosure to the instrument Required materials Chemical-resistant, powder-free gloves To fit the source enclosure to the instrument:...

  • Page 88: Operating The Source Isolation Valve

    Warning: To avoid personal contamination with biologically hazardous, toxic, and corrosive materials, wear chemical-resistant, powder-free gloves and protective eyewear when performing this procedure. Warning: To avoid puncture wounds, take great care while working with the source enclosure open if one or both of these conditions apply: •...
  • Page 89 Warning: To avoid burn injuries, take great care while working with the source enclosure open. • Open the source enclosure and wait six minutes for the isolation valve and sampling cone handles to cool before touching them. Warning: To avoid puncture wounds, take great care while working with the source enclosure open if one or both of these conditions apply: •...

  • Page 90: Opening The Source Isolation Valve

    Figure 5–3: Closing the source isolation valve Isolation valve handle in closed position Notice: To avoid damaging the StepWave assembly, do not switch the instrument into Operate mode when the isolation valve is closed. Notice: To avoid damage, do not open the source isolation valve before fitting the sampling cone assembly to the ion block assembly.

  • Page 91: Removing O-Rings And Seals

    Figure 5–4: Source isolation valve opened Isolation valve handle in open position 2. Close the source enclosure. 5.7 Removing O-rings and seals You must remove O-rings or seals from instrument components when performing certain maintenance procedures. 5.7.1 O-ring removal kit Note: The O-ring removal kit (700005054) is available to order separately.

  • Page 92: Maintaining The Source Shield Assembly

    To remove an O-ring: Notice: To avoid damaging the component when removing an O-ring or seal from it, ensure that you do not scratch the component with the removal tool. Use the tools as aids to pull the O-ring or seal from its groove. Tip: If you do not plan to reuse the O-ring or seal, you can use the forked end of tool 1 to impale the O-ring or seal to remove it.
  • Page 93 Figure 5–6: Source shield assembly Spring-loaded securing clip Cut-out (for the instrument's source pressure sensor) Rear shield Hook Front shield (for drainage) PEEK handle Figure 5–7: Source shield assembly Rear shield Front shield (for drainage, and also takes excess spray impact) November 19, 2024, 715008899 Ver.

  • Page 94: Removing The Source Shield

    5.8.1 Removing the source shield If required, remove the source shield from the instrument to perform any of the following tasks: • Clean or replace the source shield. • Fit, remove, or clean the corona pin. • Remove the sampling cone assembly from the source. •...
  • Page 95 Figure 5–8: Removing the source shield Hook PEEK handle Front shield (for drainage) 3. Holding the PEEK handle, unhook and lift away the front shield from the rear shield, ensuring that you do not flick away any residual fluid that might be present. Figure 5–9: Removing the front source shield Hook 4.

  • Page 96: Cleaning The Source Shield Components

    Figure 5–10: Removing the rear source shield Spring-loaded securing clips Pumping block Rear shield Note: To clean the shield components, see Cleaning the source shield components (Page 96). 5.8.2 Cleaning the source shield components Note: To remove the source shield components, see Removing the source shield (Page 94).

  • Page 97: Fitting The Source Shield

    Warning: To avoid personal contamination with biologically hazardous, toxic, and corrosive materials, wear chemical-resistant, powder-free gloves and protective eyewear when performing this procedure. Warning: To avoid eye injury, use eye protection when performing this procedure. 1. Immerse the shield components in separate glass vessels containing 1:1 methanol/water. 2.
  • Page 98 Figure 5–11: Fitting the rear source shield Spring-loaded securing clip Cut-out (for the instrument's source pressure sensor) Pumping block Source pressure sensor Corona pin mounting contact 2. Referring to the following figure, position section 2 of the front shield onto section 1 of the rear shield, and then slide section 4 into section 3.

  • Page 99: Cleaning The Instrument Case

    3. Slide the front shield to the left and hook it over the rear shield, as shown in the following figure, to secure it. Figure 5–13: Fitting the front source shield Hook Figure 5–14: The source shield fitted 5.9 Cleaning the instrument case Notice: To avoid abrading the surfaces of the instrument, do not use abrasives or solvents when cleaning them.
  • Page 100 Figure 5–15: Nitrogen exhaust trap bottle To laboratory exhaust port From instrument pilot valve port Bottle support Nitrogen exhaust trap bottle From instrument exhaust connection One-way valve Required materials Chemical-resistant, powder-free gloves To empty the nitrogen exhaust trap bottle: 1. Stop the LC flow. 2.

  • Page 101: Cleaning The Source Components

    Warning: To avoid personal contamination with biologically hazardous, toxic, and corrosive materials, wear chemical-resistant, powder-free gloves and protective eyewear when performing this procedure. 3. Unscrew and remove the nitrogen exhaust trap bottle from the cap and associated fittings. Warning: To avoid spreading contamination with biologically hazardous, toxic, and corrosive materials, dispose of all waste materials according to local environmental regulations.
  • Page 102 Protective eyewear To remove the sampling cone assembly from the source: Warning: To avoid personal contamination with biologically hazardous, toxic, and corrosive materials, wear chemical-resistant, powder-free gloves and protective eyewear when performing this procedure. Warning: To avoid eye injury, use eye protection when performing this procedure. Warning: To avoid puncture wounds, take great care while working with the source enclosure open if one or both of these conditions apply:...
  • Page 103 Isolation valve 3. Wait six minutes for the isolation valve, the sampling cone handle, and the source shield to cool. Warning: To avoid burn injuries, use caution when working with the isolation valve or the sampling cone handle. The surfaces close to these components can be hot.

  • Page 104: Disassembling The Sampling Cone Assembly

    Figure 5–18: Removing the sampling cone assembly Ion block assembly Notice: To avoid damaging the StepWave assembly, do not switch the instrument into Operate mode when the isolation valve is closed. Notice: To avoid damage, do not open the source isolation valve before fitting the sampling cone assembly to the ion block assembly.
  • Page 105 Figure 5–19: Cone extraction tool location Combined 2.5-mm hex wrench and cone extraction tool 2. Slide the collar to the end of the tool. Figure 5–20: Cone extraction tool Collar 3. Insert the collar in the sample cone. Figure 5–21: Inserting the cone extraction tool Insert the collar Notice: To avoid damaging the sampling cone, which is fragile, do not place it...
  • Page 106 Figure 5–22: Removing the sample cone Rotate the tool Remove the sample cone 5. Remove the O-ring from the sample cone. Figure 5–23: O-ring removed from the sample cone Cone gas nozzle Sample cone O-ring Cone gas nozzle handle Warning: To avoid spreading contamination with biologically hazardous, toxic, and corrosive materials, dispose of all waste materials according to local environmental regulations.

  • Page 107: Cleaning The Sample Cone And Cone Gas Nozzle

    5.12.3 Cleaning the sample cone and cone gas nozzle Required materials • Chemical-resistant, powder-free gloves • Protective eyewear • Appropriately sized glass vessels in which to completely immerse components when cleaning. Use only glassware not previously cleaned with surfactants. • HPLC-grade (or better) methanol •...

  • Page 108: Assembling The Sampling Cone Assembly

    a. Rinse the components by immersing them in separate glass vessels containing water, and then placing the vessels in the ultrasonic bath for 20 minutes. b. Remove any residual water from the components by immersing them in separate glass vessels containing methanol, and then placing the vessels in the ultrasonic bath for 10 minutes.

  • Page 109: Fitting The Sampling Cone Assembly To The Source

    Figure 5–24: Sampling cone assembly O-ring Sample cone Cone gas nozzle Cone gas nozzle handle Note: The PEEK handle is removable on earlier models of the cone gas nozzle assembly. If you detached the handle from the cone gas nozzle for cleaning, replace the handle and tighten it.
  • Page 110 Notice: To avoid damage, do not open the source isolation valve before fitting the sampling cone assembly to the ion block assembly. 1. Ensure that the sampling cone assembly retaining blocks are fitted to the ion block (see Fitting the sampling cone assembly retaining blocks (Page 133)).

  • Page 111: Cleaning The Ion Block Assembly

    7. Close the source enclosure. 5.13 Cleaning the ion block assembly Clean the ion block assembly if cleaning the sample cone and cone gas nozzle fails to increase signal sensitivity. 5.13.1 Removing the ion block assembly from the source assembly Required materials •...
  • Page 112 Figure 5–27: Source enclosure door open Rear shield Corona pin mounting contact blanking plug Sampling cone assembly Front shield Isolation valve 4. Remove the source enclosure, and then wait 30 minutes for the source to cool (see Removing and refitting the source enclosure (Page 85)).

  • Page 113: Disassembling The Source Ion Block Assembly

    Figure 5–28: Ion block assembly securing screws Ion block assembly securing screws 8. Remove the ion block assembly from the PEEK ion block support. Figure 5–29: Removing the ion block assembly PEEK ion block support Ion block assembly 5.13.2 Disassembling the source ion block assembly Required materials •...
  • Page 114 To disassemble the ion block assembly: Warning: To avoid personal contamination with biologically hazardous, toxic, and corrosive materials, wear chemical-resistant, powder-free gloves and protective eyewear when performing this procedure. 1. Ensure that the source isolation valve is closed. Figure 5–30: Source ion block assembly Source isolation valve handle in closed position Sampling cone assembly retaining blocks Cone gas nozzle handle...
  • Page 115 Figure 5–31: Loosening the ion block cover plate captive screws Ion block cover plate securing screws Ion block cover plate 3. Remove the ion block cover plate. 4. Grasp the cone gas nozzle handle and use it to rotate the sampling cone assembly 90 degrees, moving the handle from the vertical position to the horizontal position.
  • Page 116 7. Use the O-ring removal kit to carefully remove the isolation valve O-ring (see Removing O-rings and seals (Page 91)). Warning: To avoid spreading contamination with biologically hazardous, toxic, and corrosive materials, dispose of all waste materials according to local environmental regulations.
  • Page 117 Figure 5–34: Removing the PEEK terminal block and ceramic heater mounting block Ceramic heater mounting block PEEK terminal block 11. Use the O-ring removal kit to carefully remove the cover seal from the ion block (see also Removing O-rings and seals (Page 91)).
  • Page 118 Warning: To avoid spreading contamination with biologically hazardous, toxic, and corrosive materials, dispose of all waste materials according to local environmental regulations. 13. If the cover seal or cone gas O-ring shows signs of deterioration or damage, dispose of it in accordance with local environmental regulations.

  • Page 119: Cleaning The Ion Block Components

    Figure 5–37: Removing the sampling cone assembly retaining block screws and wave springs Wave springs Sampling cone assembly retaining block screws 15. Remove the sampling cone assembly retaining blocks from the ion block. Figure 5–38: Removing the sampling cone assembly retaining blocks Sampling cone assembly retaining blocks 5.13.3 Cleaning the ion block components Required materials...
  • Page 120 • Appropriately sized glass vessels in which to completely immerse components when cleaning. Use only glassware not previously cleaned with surfactants. • HPLC-grade (or better) methanol • HPLC-grade (or better) water • Formic acid • Ultrasonic bath • Oil-free nitrogen gas for drying (air-drying optional) •...

  • Page 121: Assembling The Source Ion Block Assembly

    4. Carefully remove the components from the vessels and blow-dry them using inert, oil-free gas. 5. Inspect each component for persisting contamination. Requirement: If contamination is present, do as follows: a. Use the wash bottle containing 1:1 methanol/water to rinse the component over the large beaker.

  • Page 122: Fitting The Ion Block Assembly To The Source Assembly

    4. Use the combined 2.5-mm hex wrench and cone extraction tool to tighten the captive PEEK terminal block securing screw. 5. Ensure that the grooves for the cover seal, cone gas O-ring, and isolation valve O-ring are free from dirt and debris. Tip: If contamination is present, use 1:1 methanol/water, applied to a lint-free cloth, to carefully clean the grooves.

  • Page 123: Cleaning The Lockspray Baffle

    Notice: To avoid recontaminating the components, wear clean, chemical-resistant, powder-free gloves. 1. Fit the ion block assembly to the PEEK ion block support. 2. Use the combined 2.5-mm hex wrench and cone extraction tool to fit, and then slowly tighten, the four ion block assembly securing screws sequentially and in small increments. 3.
  • Page 124 1. To prepare the instrument for working on the source, stop solvent flow, ensure that the instrument is in Standby mode, and stop desolvation gas flow. For further details, see Preparing the instrument for working on the source (Page 84). 2.

  • Page 125: Cleaning The Baffle

    5.14.2 Cleaning the baffle Required materials • Chemical-resistant, powder-free gloves • Protective eyewear • Appropriately sized glass vessels in which to completely immerse components when cleaning. Use only glassware not previously cleaned with surfactants. • HPLC-grade (or better) methanol • HPLC-grade (or better) water •...

  • Page 126: Fitting The Baffle

    4. Carefully remove the baffle from the vessel and blow-dry it with inert, oil-free gas. 5. Inspect the baffle for persisting contamination. If contamination is present, do as follows: a. Use the wash bottle containing 1:1 methanol/water to rinse the baffle over the large beaker.
  • Page 127 Figure 5–40: Fitting the baffle Baffle post Baffle Baffle screw slot 3. Using the 2-mm hex wrench, tighten the baffle fixing screw. Figure 5–41: Tightening the baffle Baffle screw slot Baffle 4. Fit the source enclosure. For further details, see Fitting the source enclosure to the instrument (Page 87).

  • Page 128: Replacing The Probe Assembly

    5. Close the source door. 5.15 Replacing the probe assembly Replace probe assembly if it becomes irreversibly blocked, or if it becomes contaminated or damaged. 5.15.1 Removing the probe assembly Required materials • Chemical-resistant, powder-free gloves • Protective eyewear To remove the probe assembly: Warning: To avoid personal contamination with biologically hazardous, toxic, and corrosive materials, wear chemical-resistant, powder-free gloves and protective eyewear when performing...
  • Page 129 Tip: Removing the probe adapter cap disconnects the high-voltage supply and the gas flow to the probe, and stops the source nebulizer and desolvation gas flows. Figure 5–42: Removing the probe adapter cap Probe assembly Probe adapter cap Probe adapter cap release buttons Probe adapter 4.

  • Page 130: Removing And Fitting The Sampling Cone Assembly Retaining Blocks

    7. To install a new probe assembly, see Installing the probe assembly (Page 49). Important: If you change between ESI, ESCi, and APCI modes, ensure that you fit the correct probe adapter. 5.16 Removing and fitting the sampling cone assembly retaining blocks 5.16.1 Removing the sampling cone assembly retaining blocks Remove the sampling cone assembly retaining blocks if either of these conditions applies:...
  • Page 131 1. To prepare the instrument for working on the source, stop solvent flow, ensure that the instrument is in Standby mode, and stop desolvation gas flow. For further details, see Preparing the instrument for working on the source (Page 84). 2.
  • Page 132 6. Use the combined 2.5-mm hex wrench and cone extraction tool to carefully remove the screws and wave springs that secure the sampling cone assembly retaining blocks. Figure 5–45: The sampling cone assembly retaining block screws Sampling cone assembly retaining block screws Sampling cone assembly retaining blocks Figure 5–46: Removing the sampling cone assembly retaining block screws and wave spring washers...

  • Page 133: Fitting The Sampling Cone Assembly Retaining Blocks

    Figure 5–47: Removing the sampling cone assembly retaining blocks Sampling cone assembly retaining blocks To clean the sampling cone assembly retaining blocks, see Cleaning the ion block components (Page 119). 5.16.2 Fitting the sampling cone assembly retaining blocks Fit the sampling cone assembly retaining blocks if either of these conditions applies: •...
  • Page 134 Figure 5–48: Fitting the sampling cone assembly retaining blocks Sampling cone assembly retaining blocks Slots Guiding rods 2. To secure the retaining blocks, fit a wave spring onto each retaining block screw and then use the combined 2.5-mm hex wrench and cone extraction tool to tighten them to the ion block.

  • Page 135: Replacing The Ion Block Source Heater

    5.17 Replacing the ion block source heater Replace the ion block source heater if it fails to heat the ion block when the instrument is pumped-down (evacuated). Required materials • Chemical-resistant, powder-free gloves • Needle-nose pliers • Combined 2.5-mm hex wrench and cone extraction tool •...
  • Page 136 3. Use the combined 2.5-mm hex wrench and cone extraction tool to loosen the two captive screws that secure the ion block cover plate. Figure 5–51: Loosening the ion block cover plate captive screws Ion block cover plate securing screws Ion block cover plate 4.
  • Page 137 6. Carefully remove the PEEK terminal block and ceramic heater mounting block, complete with heater cartridge assembly, from the ion block. Tip: You can invert the ion block assembly to facilitate this process. Figure 5–53: Removing the PEEK terminal block and ceramic heater mounting block Heater wire securing screws PEEK terminal block Ceramic heater mounting block...

  • Page 138: Cleaning Or Replacing The Corona Pin

    Heater cartridge assembly Warning: To avoid spreading contamination with biologically hazardous, toxic, and corrosive materials, dispose of all waste materials according to local environmental regulations. 10. Dispose of the heater cartridge assembly in accordance with local environmental regulations. Notice: To avoid damaging the heater cartridge assembly wires, do not bend or twist them when removing the assembly and ceramic heater mounting block from the ion block.

  • Page 139: Cleaning The Stepwave Ion Guide Assembly

    • Lapping film • Corona pin To clean or replace the corona pin: Warning: To avoid personal contamination with biologically hazardous, toxic, and corrosive materials, wear chemical-resistant, powder-free gloves and protective eyewear when performing this procedure. Warning: To avoid eye injury, use eye protection when performing this procedure. Warning: To avoid burn injuries, take great care while working with the source enclosure open.

  • Page 140: Handling The Stepwave Ion Guide Assembly

    5.19.1 Handling the StepWave ion guide assembly Notice: To avoid damaging the StepWave ion guide assembly, handle it and its components carefully throughout the cleaning procedure. In particular, to avoid damaging the wiring on assemblies fitted with an externally wired printed circuit board (PCB), do not touch the wiring.
  • Page 141 Figure 5–55: PEEK ion block support Housing Securing screws StepWave assembly Securing screws PEEK ion block support 4. Remove the PEEK ion block support from the adapter housing. 5. Use the O-ring removal kit to carefully remove all the O-rings from the PEEK ion block support (see Removing O-rings and seals (Page 91)).

  • Page 142: Removing The Stepwave Assembly From The Source Assembly

    5.19.3 Removing the StepWave assembly from the source assembly Required materials • Chemical-resistant, powder-free gloves • Protective eyewear • Seal-breaker and locator tool • StepWave assembly removal and insertion tool Recommendation: When not in use, store the seal breaker and locator tool on the end of the StepWave assembly removal and insertion tool.
  • Page 143 Figure 5–56: Seal breaker and locator tool Handle November 19, 2024, 715008899 Ver. 02 Page 143...
  • Page 144 Figure 5–57: Seal breaker and locator tool positioned on the adapter housing Adapter housing Ion guide cap Seal breaker and locator tool 2. Push firmly on the seal breaker and locator tool’s handle, to lever the StepWave assembly slightly out of the adapter housing. Rationale: Moving the assembly in this manner releases it from a seal located inside the instrument.
  • Page 145 Figure 5–58: StepWave assembly removal and insertion tool Ion block support screw holes (2) Brown PEEK ion guide cap Slot Cutout Pins Notice: To avoid damage when removing the StepWave ion guide assembly from the adapter housing, handle only the brown PEEK ion guide cap. 4.

  • Page 146: Disassembling The Stepwave Ion Guide Assembly

    Rationale: Fitting and closing the source enclosure prevents debris entering the instrument while you are working on the StepWave ion guide assembly. 5.19.4 Disassembling the StepWave ion guide assembly Warning: To avoid personal contamination with biologically hazardous, toxic, and corrosive materials, wear chemical-resistant, powder-free gloves and protective eyewear when performing this procedure.
  • Page 147 Figure 5–60: StepWave ion guide assembly separated Second ion guide assembly First ion guide assembly 3. Remove the brown PEEK gasket from the second ion guide assembly. Figure 5–61: StepWave second ion guide assembly Second ion guide assembly Brown PEEK gasket 4.

  • Page 148: Cleaning The Stepwave Ion Guide Assembly

    Figure 5–62: StepWave second ion guide assembly Differential pumping aperture O-ring Warning: To avoid spreading contamination with biologically hazardous, toxic, and corrosive materials, dispose of all waste materials according to local environmental regulations. 5. If the O-ring shows signs of deterioration or damage, dispose of it in accordance with local environmental regulations.
  • Page 149 • Two lengths of PEEK, PTFE, or stainless steel tubing, appropriately sized for suspending the first ion guide and second ion guide assemblies in the glass vessels when cleaning. • HPLC-grade deionized water • Waters MS Cleaning Solution (186006846) • Waste container • HPLC-grade isopropyl alcohol •...
  • Page 150 Hook First ion guide PCB assembly 3. Add Waters MS Cleaning Solution to the glass vessel until the first ion guide PCB assembly is immersed completely. 4. Repeat step 1 through step 3 for the second ion guide PCB assembly, placing the hook through one of the support rod holes.

  • Page 151: Assembling The Stepwave Ion Guide Assembly

    Tip: You can reuse the cleaning solution for one subsequent cleaning. 7. Fill the vessel with deionized water, to rinse the first ion guide PCB assembly, and then discard the water. 8. Refill the vessel with deionized water to rinse the first ion guide PCB assembly a second time, and then discard the water.
  • Page 152 Figure 5–65: Fitting the new O-ring to the differential pumping aperture Differential pumping aperture O-ring 2. Fit the brown PEEK gasket to the second ion guide assembly. Important: Ensure that the gasket is oriented correctly. Figure 5–66: Fitting the brown PEEK gasket Second ion guide assembly Brown PEEK gasket 3.

  • Page 153: Fitting The Stepwave Assembly To The Source Assembly

    4. Use the combined 2.5-mm hex wrench and cone extraction tool to fit and tighten the two screws securing the first ion guide assembly to the second ion guide assembly. 5.19.7 Fitting the StepWave assembly to the source assembly Required materials •...
  • Page 154 Figure 5–67: Sliding the StepWave assembly into the StepWave removal and insertion tool StepWave assembly Pins (2) StepWave assembly removal and insertion tool Cutout 5. With the StepWave removal and insertion tool’s cutout uppermost, insert the StepWave removal and insertion tool’s pins into the ion block support screw holes above and below the aperture in the pumping block assembly.

  • Page 155: Fitting The Ion Block Support To The Source

    Figure 5–68: Fitting the seal breaker and locator tool StepWave assembly Adapter housing Inverted seal breaker and locator tool 9. Push firmly on the seal breaker and locator tool until the tool’s face contacts the adapter housing. Rationale: This fully locates the StepWave assembly in the adapter housing. 10.

  • Page 156: Replacing The Esi Probe Tip And Gasket

    To fit the PEEK ion block support to the source: Warning: To avoid personal contamination with biologically hazardous, toxic, and corrosive materials, wear chemical-resistant, powder-free gloves and protective eyewear when performing this procedure. 1. Ensure that the grooves for the PEEK ion block support O-rings are free from dirt and debris.
  • Page 157 Warning: To avoid eye injury, use eye protection when performing this procedure. Warning: To avoid burn injuries, take great care while performing this procedure. Warning: To avoid spreading contamination with biologically hazardous, toxic, and corrosive materials, dispose of all waste materials according to local environmental regulations. 1.

  • Page 158: Fitting The Esi Probe Tip And Gasket

    5.20.2 Fitting the ESI probe tip and gasket Required materials • Chemical-resistant, powder-free gloves • 10-mm open-end wrench • 7-mm open-end wrench • New metal gasket To fit the ESI probe tip and gasket: Warning: To avoid personal contamination with biologically hazardous materials, wear clean, chemical-resistant, powder-free gloves when performing this procedure.

  • Page 159: Cleaning The Apci Probe Tip

    3. Screw the probe tip onto the ESI probe adapter. 4. Tighten the probe tip using the 7-mm wrench and the 10-mm wrench, as shown in the following figure: Figure 5–71: Tightening the probe tip ESI probe adapter 7-mm wrench 10-mm wrench ESI probe tip Important:...

  • Page 160: Replacing The Apci Probe Heater

    5. Click Operate 6. Wait 10 minutes. Rationale: The high APCI probe heater temperature removes any chemical contamination from the probe tip. 7. Click Standby 5.22 Replacing the APCI probe heater Replace the APCI probe heater if it fails to heat the probe. 5.22.1 Removing the APCI probe heater Required materials Chemical-resistant, powder-free gloves...
  • Page 161 Figure 5–72: Probe heater Probe heater Notice: To avoid damaging the probe heater's electrical connections, do not twist the heater when removing it from or refitting it to the probe adapter. Warning: To avoid burn injuries, take great care while performing this procedure.

  • Page 162: Fitting The New Apci Probe Heater

    Warning: To avoid spreading contamination with biologically hazardous, toxic, and corrosive materials, dispose of all waste materials according to local environmental regulations. 3. Dispose of the probe heater in accordance with local environmental regulations. 5.22.2 Fitting the new APCI probe heater Required materials •...

  • Page 163: Replacing The Source Assembly Seals

    Figure 5–74: Fitting the probe heater Capillary sleeve Probe heater connections Notice: To avoid damaging the probe heater's electrical connections, do not twist the heater when removing it from or refitting it to the probe adapter. 2. Fit the probe adapter to the instrument (see Installing the probe adapter (Page 46)).

  • Page 164: Removing The Probe Adjuster Assembly Probe And Source Enclosure Seals

    Warning: To avoid spreading contamination, dispose of the O-ring or seal according to local environmental regulations. O-rings and seals can be contaminated with biohazardous or toxic materials. Warning: To avoid excessive leakage of biologically hazardous or toxic solvent vapor into the laboratory atmosphere, the seals listed below must be renewed, at intervals of no greater than one year, exactly as described in this section.
  • Page 165 See also: Removing O-rings and seals (Page 91). Figure 5–75: Probe adjuster assembly seals Probe adjuster nebulizer gas seal Probe adjuster assembly probe seal 3. Use the O-ring removal kit to carefully remove the following seals from the source enclosure: •...

  • Page 166: Fitting The New Source Enclosure And Probe Adjuster Assembly Probe Seals

    Nebulizer gas seal Warning: To avoid spreading contamination with biologically hazardous, toxic, and corrosive materials, dispose of all waste materials according to local environmental regulations. 4. Dispose of all the seals in accordance with local environmental regulations. 5.23.2 Fitting the new source enclosure and probe adjuster assembly probe seals Required materials •...

  • Page 167: Replacing The Mass Spectrometer's Air Filters

    Figure 5–77: Fitting the seal into the groove Seal Groove 4. Fit the following new seals to the probe adjuster assembly: • Probe seal • Nebulizer gas seal 5. Fit the source enclosure to the instrument (see Fitting the source enclosure to the instrument (Page 87)).
  • Page 168 Required materials • New air filters Figure 5–78: Xevo MRT lower front panel attached Left- and right-side drain tube guides Lower front panel To replace the air filters: 1. Remove the lower front panel from the instrument by pulling gently on both sides of it simultaneously to overcome the magnetic fixings on the drain tube guides holding it in place.

  • Page 169: Replacing The Lockspray Ii Reference Probe

    5.25 Replacing the LockSpray II reference probe The LockSpray II reference probe differs from the original LockSpray reference probe in that it comes as one complete unit, pre-built to length. The capillary end connects to the reference sprayer assembly within the LockSpray II source housing. Important: There is no LockSpray II retrofit option for the LockSpray source, but the front-end housings are interchangeable on the instrument.

  • Page 170: Removing The Existing Reference Probe

    Reference probe If your system is supplied with, or your application requires the use of a microfilter, fit it to the PEEK inlet of the reference probe. The microfilter is provided with the manufacturer’s fitting instructions in the spares kit for the reference probe assembly. Warning: To avoid risk of electric shock, switch the instrument to Standby before working on the reference sprayer connection.
  • Page 171 To remove the existing one-piece reference probe: 1. To prepare the instrument for working on the source, stop solvent flow, ensure that the instrument is in Power Save, and then stop desolvation gas flow. For further details, see Preparing the instrument for working on the source (Page 84).
  • Page 172 Warning: To avoid burn injuries, take great care while working with the probe and source; these components can be hot. Figure 5–83: Removal of the reference probe inlet connector from within the source housing Source housing Reference probe inlet connector 5.
  • Page 173 Reference sprayer assembly 6. Unscrew the finger-tight fitting counterclockwise to remove the capillary end of the reference probe from the reference sprayer assembly. Figure 5–85: Unscrewing the finger-tight fitting from the reference sprayer assembly Reference sprayer assembly Finger-tight fitting 7. Remove the reference sprayer probe capillary from the reference sprayer assembly. Figure 5–86: Removal of the reference sprayer capillary from the sprayer assembly Reference sprayer assembly Reference sprayer probe capillary...

  • Page 174: Installing The New Reference Probe

    Warning: To avoid spreading contamination with biologically hazardous, toxic, and corrosive materials, dispose of all waste materials according to local environmental regulations. 8. Dispose of the used reference sprayer probe in accordance with local environmental regulations. 5.27 Installing the new reference probe Required materials •...
  • Page 175 Figure 5–88: Securing the reference sprayer capillary Reference sprayer assembly Finger-tight fitting 3. Using the 4-mm open-end wrench, adjust the reference sprayer to ensure the correct capillary protrusion as required. 4. Insert the reference sprayer assembly into its support assembly with the reference sprayer tip pointing upward.
  • Page 176 Locating notch on sprayer assembly Reference sprayer assembly stem 5. Rotate the reference sprayer assembly 90 degrees counterclockwise to lock it in place. Figure 5–90: Locking the reference sprayer assembly 6. Rotate the reference probe inlet connector until its groove aligns with the source housing notch.
  • Page 177 Groove in reference probe PEEK inlet connector 8. Push the reference probe inlet connector upwards until the bayonet groove is above the notch. 9. Rotate the reference probe inlet connector until the bayonet groove aligns with the notch on the source housing. Figure 5–92: Alignment of notch and groove Source housing notch Bayonet groove...

  • Page 178: Replacing The Nanolockspray Reference Probe Tapertip Emitter Or Capillary

    5.28 Replacing the NanoLockSpray reference probe TaperTip emitter or capillary Replace the NanoLockSpray reference-probe TaperTip emitter or capillary if either is irreversibly blocked, contaminated, or damaged. 5.28.1 Removing the NanoLockSpray reference probe Required materials: • Chemical-resistant, powder-free gloves • Combined 2.5-mm hex wrench and cone extraction tool •...
  • Page 179 Figure 5–94: Removing the NanoLockSpray reference probe's fixing screws Fixing screw NanoLockSpray reference probe Warning: To avoid puncture wounds, handle the probe with care. The reference-probe tip is an exposed, fused-silica TaperTip emitter that is sharp and fragile. 7. Remove the NanoLockSpray reference probe from the probe adjuster assembly. 8.

  • Page 180: Installing The New Tapertip Emitter And Capillary

    Capillary Union TaperTip emitter TaperTip-emitter outlet Warning: To avoid eye injury from fused silica lines, wear protective eyewear. 9. Unscrew the capillary PEEK coupler, and remove the capillary from the union. 10. Where appropriate, remove the protective PEEK sleeve from the capillary for reuse. Warning: To avoid spreading contamination with biologically hazardous, toxic, and corrosive materials, dispose of all waste materials according to local environmental...
  • Page 181 • Loosen the set screw, using the 1.5-mm hex wrench. • Reposition the union so the surface is level with the bottom of the body holder. • Tighten the set screw, using the 1.5-mm hex wrench. Figure 5–96: NanoLockSpray reference probe Set screw Union Body holder...

  • Page 182: Maintaining The Valve Pods

    (Page 87)). 14. Using an F-130 "finger-tight" nut, connect the 75-µm sleeved fused-silica capillary to port 1 of the Xevo MRT diverter valve. 15. Connect the NanoLockSpray reference probe’s cable to the instrument’s high-voltage connector. 5.29 Maintaining the valve pods With prolonged use, the surfaces that are regularly exposed to the solutions within the instrument valves (the reference selector and diverter valves) can become damaged.
  • Page 183 Figure 5–97: Removing the valve pod locking ring Locking ring Selector valve pod (on the WRDS unit) Locking ring Diverter valve pod (on the Xevo MRT MS) 4. Remove the valve pod from the valve pod housing. November 19, 2024, 715008899 Ver. 02 Page 183...

  • Page 184: Installing The Valve Pod Into The Instrument

    Figure 5–98: Removing the valve pod Note: For illustrative purposes, the diverter valve pod is shown. Diverter valve pod housing Diverter valve pod 5. If you are replacing the valve pod, dispose of the old valve pod according to local environmental regulations.
  • Page 185 1. Before you install the valve pod, note the profile on the valve pod union and how it connects to the corresponding fitting inside the valve pod housing. Note: For illustrative purposes, the diverter valve pod is shown. Figure 5–99: Installing the valve pod Groove Diverter valve pod housing Valve pod union...

  • Page 186: Replacing The Pod's Isolation Seal And Rotor Seal

    Figure 5–100: Fitting the locking ring Locking ring 6. Fit and tighten the PEEK thumbscrews to the valve or valves of interest, and then attach the tubes as indicated by the tubing schematic on the instrument. 7. Purge the solvent line of interest through the valve pod at least twice. 5.29.3 Replacing the pod's isolation seal and rotor seal Required materials Chemical-resistant, powder-free gloves...
  • Page 187 2. Remove the valve pod of interest as described in Removing the valve pods from the instrument (Page 182). 3. Using the T20 TORX driver, loosen and remove the three stator screws. Note: For illustrative purposes, the diverter valve pod is shown. Figure 5–101: Valve pod stator screws Stator screws 4.
  • Page 188 Figure 5–102: Valve pod assembly Rotor seal mount Isolation seal Rotor seal Stator ring Stator face assembly Stator Stator screws 5. Remove the rotor seal and isolation seal from the rotor seal mount and dispose of them according to local environmental regulations. November 19, 2024, 715008899 Ver.
  • Page 189 6. Fit the new isolation seal to the rotor seal mount, and then fit the new rotor seal. Figure 5–103: Fitting the isolation seal and rotor seal Rotor seal mount Isolation seal Rotor seal 7. In this sequence, fit the stator ring, stator face assembly, and stator. 8.

  • Page 190: A Safety Advisories

    Heed all warnings when you install, repair, or operate any Waters instrument or device. Waters accepts no liability in cases of injury or property damage resulting from the failure of individuals to comply with any safety precaution when installing, repairing, or operating any of its instruments or devices.

  • Page 191: Specific Warnings

    (Risk of high-pressure gas release.) A.1.1 Specific warnings A.1.1.1 Burst warning This warning applies to Waters instruments and devices fitted with nonmetallic tubing. Warning: To avoid injury from bursting, nonmetallic tubing, heed these precautions when working in the vicinity of such tubing when it is pressurized: •...
  • Page 192 A.1.1.4 Biohazard warning The following warning applies to Waters instruments and devices that can process biologically hazardous materials. Biologically hazardous materials are substances that contain biological agents capable of producing harmful effects in humans.

  • Page 193: Notices

    Warning: To avoid personal contamination with biologically hazardous, toxic, or corrosive materials, you must understand the hazards associated with their handling. Guidelines prescribing the proper use and handling of such materials appear in the latest edition of the National Research Council's publication, Prudent Practices in the Laboratory: Handling and Management of Chemical Hazards.

  • Page 194: Warnings That Apply To All Waters Instruments And Devices

    A.5 Warnings that apply to all Waters instruments and devices When operating this device, follow standard quality-control procedures and the equipment guidelines in this section. Warning: Changes or modifications to this unit not expressly approved by the party responsible for compliance could void the user’s authority to operate the equipment.
  • Page 195 Warning: Use caution when working with any polymer tubing under pressure: • Always wear eye protection when near pressurized polymer tubing. • Extinguish all nearby flames. • Do not use tubing that has been severely stressed or kinked. • Do not use nonmetallic tubing with tetrahydrofuran (THF) or concentrated nitric or sulfuric acids.
  • Page 196 Avvertenza: Fare attenzione quando si utilizzano tubi in materiale polimerico sotto pressione: • Indossare sempre occhiali da lavoro protettivi nei pressi di tubi di polimero pressurizzati. • Spegnere tutte le fiamme vive nell'ambiente circostante. • Non utilizzare tubi eccessivamente logorati o piegati. •...
  • Page 197 • 非金属チューブには、テトラヒドロフラン (THF) や高濃度の硝酸または硫酸など を流さないでください。 • 塩化メチレンやジメチルスルホキシドは、非金属チューブの膨張を引き起こす場合 があり、その場合、チューブは極めて低い圧力で破裂します。 This warning applies to Waters instruments fitted with nonmetallic tubing or operated with flammable solvents. Warning: The user shall be made aware that if the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired.

  • Page 198: Warnings That Address The Replacement Of Fuses

    警告： 使用者必須非常清楚如果設備不是按照製造廠商指定的方式使用，那麼該設備 所提供的保護將被消弱。 경고 제조업체가 명시하지 않은 방식으로 장비를 사용할 경우 장비가 제공하는 보호 수단이 제대로 작동하지 않을 수 있다는 점을 사용자에게 반드시 인식시켜야 합니다. 警告 ユーザーは、製造元により指定されていない方法で機器を使用すると、機器が 提供している保証が無効になる可能性があることに注意して下さい。 A.6 Warnings that address the replacement of fuses The following warnings pertain to instruments and devices equipped with user-replaceable fuses.

  • Page 199: Electrical Symbols

    警告 火災予防のために、ヒューズを交換する場合は、装置ヒューズカバーの隣のパ ネルに記載されている種類および定格のヒューズをご使用ください。 Finding fuse types and ratings when that information does not appear on the instrument or device: Warning: To protect against fire, replace fuses with those of the type and rating indicated in the “Replacing fuses” section of the Maintenance Procedures chapter. Avertissement : Pour éviter tout risque d’incendie, remplacez toujours les fusibles par d’autres du type et de la puissance indiqués dans la rubrique «...

  • Page 200: Handling Symbols

    Symbol Description Electrical power off Standby Direct current Alternating current Alternating current (three phase) Safety ground Frame or chassis terminal connection Fuse Functional ground Input Output Indicates that the device or assembly is susceptible to damage from electrostatic discharge (ESD) A.8 Handling symbols The following handling symbols and their associated statements can appear on labels affixed to the packaging in which instruments, devices, and component parts are shipped.
  • Page 201 Symbol Description Fragile! Use no hooks! Upper limit of temperature Lower limit of temperature Temperature limitation November 19, 2024, 715008899 Ver. 02 Page 201...

  • Page 202: B External Connections

    To avoid damaging the mass spectrometer, observe the following precautions: • Contact Waters Technical Service before moving the instrument. • If you must transport the instrument, or remove it from service, contact Waters Technical Service for recommended cleaning, flushing, and packaging procedures.
  • Page 203 Figure B–1: Mass spectrometer rear panel connectors EXTERNAL CONNECTIONS EVENT GAS FAIL EVENT 1 EVENT 2 Vacuum port Power switch Backing Main connection ADC ref in Collision gas (Nitrogen) Video out Comms ADC in 1 ADC in 2 Manual Pump/Vent ADC trig in API gas (Nitrogen) External connections...

  • Page 204: Connecting The Dry Vacuum Pump

    Pilot valve RDS connector B.2 Connecting the dry vacuum pump Rough pumping of the Xevo MRT is provided by an EBARA EV-SA20 dry vacuum pump. For proper ventilation, the following minimum clearances must apply: Figure B–2: EBARA dry vacuum pump clearances...
  • Page 205 • NW25 center rings • NW25 clamps • NW40 center rings • NW40 clamps • NW40 elbow • NW40 tee piece • NW40 to NW25 reducer • 12.7-mm clear PVC exhaust tubing • PVC hose clamps • Vacuum hose To connect the dry vacuum pump: Warning: To avoid personal contamination with biologically hazardous or toxic compounds, wear clean, chemical-resistant, powder-free gloves when performing this procedure.
  • Page 206 Figure B–3: Vacuum pump tubing connection to the rear of the mass spectrometer VACUU NW 40 clamp Vacuum port November 19, 2024, 715008899 Ver. 02 Page 206...
  • Page 207 Figure B–4: Vacuum pump tubing connections to the EBARA pump NW40 clamp Vacuum hose (from the rear of the Xevo MRT) Exhaust tubing NW40 clamp Exhaust silencer Gas ballast plug NW40 clamp and exhaust filter (supplied with the EBARA vacuum pump...

  • Page 208: Making The Electrical Connections To The Dry Vacuum Pump

    To repair or replace the pump, contact Waters Technical Support. For more information, see Contacting Waters (Page iii). B.3 Making the electrical connections to the dry vacuum pump Figure B–5: Vacuum pump electrical connections to the rear of the Xevo MRT mass spectrometer FLUIDICS BACKING EXTERNAL CONNECTIONS +(1)

  • Page 209: Connecting To The Nitrogen Gas Supply

    B.4 Connecting to the nitrogen gas supply Required materials • Chemical-resistant, powder-free gloves • 6-mm PTFE tubing (included in the Xevo MRT Installation Kit) • Nitrogen regulator (not supplied) Warning: To prevent ignition of flammable solvents in the source enclosure, always use nitrogen of at least 95% purity as the API gas.

  • Page 210: Connecting The Nitrogen Exhaust Line

    B.6 Connecting the nitrogen exhaust line Required materials • Chemical-resistant, powder-free gloves • Utility knife • Nitrogen exhaust trap bottle • 4-mm PTFE tubing and 12-mm PTFE tubing (included in the Xevo MRT installation kit) November 19, 2024, 715008899 Ver. 02 Page 210...
  • Page 211 Waters recommends that the exhaust system include the capability to drain off any solvent accumulation, or that the exhaust system is designed to prevent condensation, for example, by being open and maintaining a gas flow of at least 5000 L/hour through the exhaust system.
  • Page 212 Figure B–6: Nitrogen exhaust trap bottle To laboratory exhaust port From instrument pilot valve port Bottle support Nitrogen exhaust trap bottle From instrument exhaust connection One-way valve Notice: To avoid gas leaks, use the tube cutter to cut the PTFE tubing squarely. 2.

  • Page 213: Connecting Liquid Waste Lines

    3. Connect waste lines to the diverter valve and the source waste outlet connections on the front of the instrument as shown in the image below. Note: The Xevo MRT waste line is routed to either the left- or right-hand side of the instrument. November 19, 2024, 715008899 Ver. 02...
  • Page 214 4. Route the waste lines to the waste container. If necessary, shorten the waste tubes so that their ends are above the surface of the waste solvent. Note: The waste line from the Waters RDS is routed externally, directly to the waste container (see ). (Page 22) November 19, 2024, 715008899 Ver.

  • Page 215: Connecting The Workstation (Systems With No Acquity Lc)

    Figure B–8: Positioning the drain tube Correct Incorrect B.8 Connecting the workstation (systems with no ACQUITY Before connecting the workstation to the instrument, set up the workstation according to its accompanying instructions. Locate the workstation within three meters (ten feet) of the mass spectrometer.

  • Page 216: Connecting Ethernet Cables (Systems With Acquity Lc)

    B.9 Connecting Ethernet cables (systems with ACQUITY LC) Requirement: Use shielded Ethernet cables with the mass spectrometer to ensure compliance with FCC limits. To make Ethernet connections: 1. Connect one end of one shielded Ethernet cable to the ACQUITY instrument’s network switch, and then connect the free end to the Ethernet card on the pre-configured ACQUITY workstation.
  • Page 217 Figure B–9: Input and output connector locations FLUIDICS BACKING EXTERNAL CONNECTIONS +(1) +(2) EVENT GAS FAIL EVENT 1 EVENT 2 External connectors External connections identification tables Figure B–10: Input/output signal connector configuration Table B–1: External connections Function Rating EVENT IN +(1) 5.4 V , <1 Ohm EVENT IN +(2) 5.4 V , <1 Ohm...

  • Page 218: Signal Connections

    Table B–1: External connections (continued) Function Rating EVENT 2 Out - 27 V, <1 Ohm B.10.1 Signal connections Table B–2: Mass spectrometer signal connections Signal connections Description Event IN +(1), Event IN +(2) Allows an external device to start data acquisition.
  • Page 219 Figure B–11: Attaching the signal cable leads to the connector Connector Signal cables 3. Slide the clamp (with the bend facing down) into the protective shield. 4. Insert the clamp and shield (with the bend facing down) into the connection cover and loosely tighten with one self-tapping screw.

  • Page 220: Connecting The Contact-Closure Cable To An Acquity Lc

    Figure B–13: Inserting the connector Cable leads Clamp 6. Place the second connection cover over the first cover and snap it into place. Figure B–14: Joining the covers Signal connector Connection cover B.11 Connecting the contact-closure cable to an ACQUITY LC The contact-closure cable connects the mass spectrometer’s Gas Fail Interlock signal connectors to the ACQUITY LC system’s Stop Flow In signal connectors.
  • Page 221 To connect the contact-closure cable: 1. Connect the leads at one end of the contact-closure cable to the Xevo MRT’s signal connectors, as shown in the following table. Table B–3: Xevo MRT contact-closure cable connections...

  • Page 222: Connecting To The Electricity Source

    HAR-type (or better) cords in Europe. The power cords must be replaced only with ones of adequate rating. For information regarding which cord to use in other countries, contact your local Waters distributor. 1. Connect the female end of the power cord to the receptacle on the rear panel of the mass spectrometer.

  • Page 223: Installing The Camera Driver Software

    Prerequisites: • You created an instrument system in the software. • You fitted a NanoLockSpray source to the Xevo MRT MS. To Install the camera driver software: 1. Connect the Video-to-USB converter to the PC.

  • Page 224: C Materials Of Construction And Compatible Solvents

    C.1 Preventing contamination For information on preventing contamination, refer to Controlling Contamination in LC/MS Systems (715001307), which is available at www.waters.com. C.2 Items exposed to solvent The following table lists the items that can be exposed to solvent. You must evaluate the safety issues if the solvents used in your application differ from the solvents typically used with these items.

  • Page 225: Solvents Used To Prepare Mobile Phases

    Table C–1: Items exposed to solvent (continued) Item Material O-rings Viton or PTFE-encapsulated Viton Probe adjuster bellows Viton Probe capillary Stainless steel and fused silica Probe shaft PEEK Pumping block Aluminum Source enclosure Aluminum Source enclosure view port Silica float glass Trap bottle Polypropylene Trap bottle push-in fittings...
  • Page 226 • Isopropanol • Propanol • Trifluoroacetic acid (≤0.1%) • Hexafluoroisopropanol (≤5%) • Triethylamine (≤0.1%) These solvents are not expected to adversely affect performance of the materials shown in the preceding table. November 19, 2024, 715008899 Ver. 02 Page 226...

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