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Waters Xevo TQ-XS IVD Overview And Maintenance Manual

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Waters Xevo TQ-XS IVD Mass
Spectrometer
Overview and Maintenance Guide
715004990IVD
Copyright © Waters Corporation 2019
Revision A
All rights reserved

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Summary of Contents for Waters Xevo TQ-XS IVD

  • Page 1 Waters Xevo TQ-XS IVD Mass Spectrometer Overview and Maintenance Guide 715004990IVD Copyright © Waters Corporation 2019 Revision A All rights reserved...
  • Page 2 General information Copyright notice © 2019 WATERS CORPORATION. PRINTED IN THE UNITED STATES OF AMERICA AND IN IRELAND. ALL RIGHTS RESERVED. THIS DOCUMENT OR PARTS THEREOF MAY NOT BE REPRODUCED IN ANY FORM WITHOUT THE WRITTEN PERMISSION OF THE PUBLISHER.
  • Page 3 All other trademarks are property of their respective owners. Contacting Waters Contact Waters with enhancement requests or technical questions regarding the use, transportation, removal, or disposal of any Waters product. You can reach us via the Internet, telephone, fax, or conventional mail. Waters contact information...
  • Page 4 For information regarding which cord to use in other countries, contact your local Waters distributor. Solvent leakage hazard The source exhaust system is designed to be robust and leak-tight. Waters recommends that you perform a hazard analysis, assuming a maximum leak into the laboratory atmosphere of 10% LC eluate.
  • Page 5 Flammable solvents hazard Warning: To prevent the ignition of flammable solvent vapors in the enclosed space of a mass spectrometer’s ion source, ensure that these conditions are met: • Nitrogen flows continuously through the source. • A gas-fail device is installed to interrupt the flow of LC solvent should the nitrogen supply fail.
  • Page 6 Do not dispose of the instrument or return it to Waters for repair until the authority responsible for approving its removal from the premises specifies the extent of decontamination required and the level of residual contamination permissible.
  • Page 7 Equipment misuse notice If equipment is used in a manner not specified by its manufacturer, the protection provided by the equipment may be impaired. Safety advisories Consult the "Safety advisories" appendix in this publication for a comprehensive list of warning advisories and notices.
  • Page 8 Intended use Waters designed the Xevo TQ-XS IVD for use with the ACQUITY UPLC I-Class/Xevo TQ-XS IVD system to perform mass spectrometry for in vitro diagnostic use.
  • Page 9 Directive 98/79/EC. Important: The Xevo TQ-XS IVD is fitted with a Waters ESI source. Do not fit the Xevo TQ-XS IVD with other Waters or third-party sources. The Xevo TQ-XS IVD does not comply with the European Union In Vitro Diagnostic Device Directive 98/79/EC when fitted with other sources.
  • Page 10 • Use one or more internal standard compounds, preferably isotopically labeled analytes. • To ensure data integrity, Waters recommends that you run QC samples before and after test samples within a single analysis. Also, you can periodically run known or PT/EQA samples as unknowns to verify assay results for a single analysis.
  • Page 11 To avoid difficulties in ensuring electromagnetic compatibility, if the instrument's pump control cable is attached to the vacuum hose, ensure that the cable is grounded to the mass spectrometer. Manufacturing information Manufacturer: Waters Corporation 34 Maple Street Milford, MA 01757 Australian sponsor information Australian Sponsor...
  • Page 12 Manufacturing information ........................xi Australian sponsor information ......................xi 1 Waters Xevo TQ-XS IVD Overview ................17 1.1 IntelliStart technology........................17 1.2 ACQUITY UPLC I-Class/Xevo TQ-XS IVD System ..............18 1.2.1 ACQUITY UPLC I-Class IVD system .................. 18 1.2.2 Software and data system ....................20 1.3 Electrospray ionization (ESI)......................21 September 11, 2019, 715004990IVD Rev.
  • Page 13 1.4 IntelliStart fluidics system......................21 1.4.1 Overview..........................21 1.4.2 System components ......................22 1.4.3 System operation......................... 22 1.5 Ion optics............................23 1.6 MS operating modes ........................24 1.7 MS/MS operating modes....................... 24 1.7.1 Product (daughter) ion spectrum ..................25 1.7.2 Precursor (parent) ion spectrum ..................26 1.7.3 MRM mode .......................... 26 1.7.4 Constant neutral loss mode ....................
  • Page 14 2.10 Leaving the mass spectrometer ready for operation..............38 2.11 Emergency shutdown of the mass spectrometer ................ 39 3 Installing and removing the ESI probe ..............40 3.1 Installing the probe adapter......................40 3.2 Installing the probe assembly......................43 3.3 Removing the probe adapter......................49 4 Maintenance procedures ..................51 4.1 Maintenance Schedule........................
  • Page 15 A.2 Notices............................112 A.3 Bottles Prohibited symbol ......................112 A.4 Required protection ........................112 A.5 Warnings that apply to all Waters instruments and devices ............113 A.6 Warnings that address the replacement of fuses................ 117 A.7 Electrical symbols ........................118 September 11, 2019, 715004990IVD Rev. A...
  • Page 16 A.8 Handling symbols ........................119 B External connections.....................121 B.1 External wiring and vacuum connections ................... 121 B.2 Connecting the EBARA roughing pump ..................122 B.3 Making the electrical connections to the Ebara oil-free roughing pump ........126 B.4 Connecting to the nitrogen gas supply ..................127 B.5 ...
  • Page 17 Waters Xevo TQ-XS IVD Overview The Xevo TQ-XS IVD is a tandem quadrupole, atmospheric pressure ionization (API) mass spectrometer. It is designed for routine UPLC/MS/MS analyses in quantitative and qualitative applications and can operate at fast acquisition speeds compatible with UltraPerformance LC.
  • Page 18 Click Services & Support > Support Library. In Waters documents, the term “fluidics” refers to the IntelliStart Fluidics system, which is the instrument’s onboard system that delivers sample and solvent to the probe of the mass spectrometer. It can also denote plumbing components and fluid pathways within and between system modules.
  • Page 19 Figure 1–2: ACQUITY UPLC I-Class/Xevo TQ-XS IVD system Bottle tray Access door to the fluidics valve Removable panel for ACQUITY arm Probe high voltage connector Source interface sliding door Probe adapter Source enclosure Source enclosure release September 11, 2019, 715004990IVD Rev. A...
  • Page 20 Xevo TQ-XS IVD Binary Solvent Manager IVD (BSM) Sample Manager FTN IVD Column Heater 1.2.2 Software and data system MassLynx software controls the mass spectrometer and the TargetLynx XS and IonLynx application managers are included as standard software. MassLynx software acquires, analyzes, manages, and distributes mass spectrometry data and enables these major operations: •...
  • Page 21 1.3 Electrospray ionization (ESI) In electrospray ionization (ESI), a strong electrical charge is applied to the eluent as it emerges from a nebulizer. The droplets that compose the resultant aerosol undergo a reduction in size (solvent evaporation). As solvent continues to evaporate, the charge density increases until the droplet surfaces eject ions (ion evaporation).
  • Page 22 Reservoir C Reservoir B Reservoir A Pump Wash bottle, located in solvent tray To waste system Column Diverter valve Probe 7-port selector valve 1.4.2 System components The onboard system incorporates a 7-port selector valve, a multi-position diverter valve, a pump, and three sample reservoirs.
  • Page 23 1.5 Ion optics The mass spectrometer’s ion optics operate as follows: Samples from the LC or instrument’s solvent delivery system are introduced at atmospheric pressure into the ionization source, where they are ionized. The ions pass through the sample cone into the vacuum system. The resulting ion beam passes through the source sampling orifice, undergoing a certain amount of expansion.
  • Page 24 Quadrupole 1 (MS1) T-Wave/ScanWave collision cell Quadrupole 2 (MS2) Detector assembly - Conversion dynode Detector assembly - Detector Z-Spray ion source - Source sampling orifice Z-Spray ion source - Isolation valve 1.6 MS operating modes The following table shows the MS operating modes. Table 1–1: MS operating modes: Operating mode Collision cell...
  • Page 25 Table 1–2: MS/MS operating modes: Operating mode Collision cell Product (daughter) ion Static (at precursor Fragment precursor Scanning spectrum mass) ions and pass all masses Precursor (parent) ion Scanning Static (at product spectrum mass) Static (at precursor Static (at product mass) mass) Constant neutral loss...
  • Page 26 1.7.1.1 Typical applications You typically use product ion spectrum for the following applications: • Method development for MRM screening studies: • Identifying product ions for use in MRM transitions. • Optimizing CID tuning conditions to maximize the yield of a specific product ion to be used in MRM analysis.
  • Page 27 Figure 1–7: MRM mode MS1–Static (at precursor mass) Collision cell–Fragment precursor ions and pass all masses MS2–Static (at product mass) 1.7.3.1 Typical application You typically use MRM mode to quantify known analytes in complex samples: • environmental studies; for example, pesticide and herbicide analysis •...
  • Page 28 MS1–Scanning (synchronized with MS2) Collision cell–Fragment precursor ions and pass all masses MS2–Scanning (synchronized with MS1) 1.7.4.1 Typical application You typically use constant neutral loss mode to screen mixtures for a specific class of compound that is characterized by a common fragmentation pathway, indicating the presence of compounds containing a common functional group.
  • Page 29 1.5 mL of accumulated leaked liquid in its surrounding reservoir. At the same time, the software displays an error message alerting you that a leak has developed. Consult the Waters ACQUITY UPLC Leak Sensor Maintenance Instructions (71500082506) for complete details.
  • Page 30 Figure 1–10: Mass spectrometer rear panel LA N External C onnections1 Service Bus EPC Com Port External C onnections 2 Video Output OUT - External Connections 1 External Connections 2 Auxiliary 10MB Activity /100MB Shielded Ethernet Video connection (not used) Event inputs and outputs Power connection Roughing pump connections...
  • Page 31 Preparing the mass spectrometer for operation This chapter describes how to start and shut down the mass spectrometer. 2.1 Preparing to start the mass spectrometer Notice: To avoid causing severe damage to the instrument, use only compatible solvents. See also: For more details, refer to the following sources: •...
  • Page 32 2.2 Starting the mass spectrometer Starting the mass spectrometer comprises powering-on the workstation, logging in, powering-on the mass spectrometer and all other instruments, and then starting the software. Requirements: • Ensure that you have prepared the mass spectrometer. See Preparing to start the mass spectrometer.
  • Page 33 • In the Instrument Console, the System Ready indicator shows green when the instrument is fully pumped-down (evacuated). • Expect the Analyzer Penning gauge readback to show less than 1e-5 mbar vacuum. Warning: To prevent the ignition of flammable solvent vapors in the enclosed space of a mass spectrometer’s ion source, ensure that these conditions are met: •...
  • Page 34 The ACQUITY UPLC system runs at high flow rates. To optimize desolvation and sensitivity, run the ACQUITY Xevo TQ-XS IVD system at appropriate gas flows and desolvation temperatures. When you specify a flow rate, IntelliStart software automatically specifies the settings shown in the following table.
  • Page 35 2.7 Preparing the IntelliStart Fluidics system For additional information, see Connecting liquid waste lines. Prohibited: To avoid equipment damage caused by spilled solvent, do not place reservoir bottles directly atop an instrument or device or on its front ledge. Instead, place the bottles in the bottle tray, which serves as secondary containment in the event of spills.
  • Page 36 2.7.2 Installing the low-volume vials Warning: To avoid personal contamination with biologically hazardous, toxic, or corrosive materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when performing this procedure. To install the low-volume vials: If a standard reservoir bottle is fitted, remove it. Screw the low-volume adapters into the manifold and finger-tighten them.
  • Page 37 To adjust the position of a solvent delivery tube: Open the access door to the fluidics pump (see ACQUITY UPLC I-Class/Xevo TQ-XS IVD system). Loosen the finger-tight fitting for the solvent delivery tube you are adjusting. Finger-tight fitting Solvent delivery tube Move the solvent delivery tube so that its end is close to, but does not touch, the bottom of the reservoir bottle or low volume vial.
  • Page 38 • The software fails to establish communication or loses communication with the mass spectrometer. • You perform a software upgrade. To reboot the mass spectrometer: Ensure that the mass spectrometer software is closed. Open the mass spectrometer’s front, left-hand door. Insert a short length (7.5 cm) of PEEK tubing, or similar object, into the reset button aperture to operate the reset button.
  • Page 39 2.11 Emergency shutdown of the mass spectrometer Warning: To avoid electric shock, observe the following procedure to isolate the instrument from the main power supply. The instrument's power switch does not isolate it from the main power supply. Notice: To avoid losing data, use the following procedure only in an emergency. To reboot the mass spectrometer, follow the procedure in the section "Rebooting the mass spectrometer".
  • Page 40 Installing and removing the ESI probe The following sections explain how to install and remove an ESI probe. For further details on running ESI applications, see Electrospray ionization (ESI). 3.1 Installing the probe adapter Figure 3–1: Probe adapter parts Probe adapter cap removed from probe adapter Probe adapter cap tether Locking ring Probe adapter identification label...
  • Page 41 Required materials • Chemical-resistant, powder-free gloves To install the probe adapter: Warning: To avoid personal contamination with biologically hazardous or toxic compounds, wear clean, chemical-resistant, powder-free gloves when performing this procedure. Warning: To avoid puncture wounds, handle sharp parts and materials with care. Remove the protective cap, if fitted, from the probe tip.
  • Page 42 Figure 3–4: Locating the ESI probe adapter Location hole for probe location dowel Probe adjuster assembly Rotate the probe adapter locking ring clockwise to secure the probe adapter in place. Figure 3–5: Probe adapter mounted on the source enclosure Probe adapter cap Probe adapter locking ring Probe adjuster assembly Source enclosure...
  • Page 43 Connect the ESI probe adapter’s cable to the high-voltage connector. Install the probe assembly. See Installing the probe assembly. 3.2 Installing the probe assembly Requirements: • Ensure that the probe adapter is installed on the source, with the probe adapter cap removed. Installing the probe adapter.
  • Page 44 Required materials • Chemical-resistant, powder-free gloves To install the probe assembly: Warning: To avoid personal contamination with biologically hazardous or toxic compounds, wear clean, chemical-resistant, powder-free gloves when performing this procedure. Warning: To avoid electric shock, do not insert any item into the probe cap aperture when the probe cap is fitted to the instrument.
  • Page 45 Figure 3–8: Inserting the probe assembly Probe assembly capillary Probe adapter Screw the probe adapter fitting into the probe adapter, finger-tight only, until you hear a click. Figure 3–9: Probe assembly fitted to the probe adapter Probe adapter fitting Tip: The probe adapter fitting varies in size depending on the probe assembly type.
  • Page 46 the correct probe assembly. For example, the Unispray probe assembly will not fit the tool- free probe adapter. Tilt the probe adapter cap so that the ball bearing is located in the recess at the bottom of the aperture, and then insert the probe assembly tubing through the aperture. Figure 3–10: Probe adapter cap Probe cap aperture from the underside Probe cap aperture from the top...
  • Page 47 Figure 3–11: Fitting the probe adapter cap Probe adapter cap Probe adapter cap tether Probe adapter Probe assembly tubing Slide the probe adapter cap along the probe assembly, over the probe adapter inlet fitting. Push the probe adapter cap onto the probe adapter until it clicks. Tips: •...
  • Page 48 Figure 3–12: Seating the probe adapter cap Probe adapter cap seated incorrectly: edge does not align with the edge of the probe adapter Probe adapter cap seated correctly: edge aligns with the edge of the probe adapter Note: If you fit the probe adapter cap when the source enclosure is closed and the mass spectrometer is in Operate mode, the high-voltage supply to the probe turns on and the instrument performs a pressure test.
  • Page 49 Figure 3–13: Inlet fitting holder Probe inlet fitting Inlet fitting holder Probe assembly tubing Connect the probe inlet fitting to the inlet system. 3.3 Removing the probe adapter Remove the probe adapter before replacing the ESI probe tip or gasket (see Replacing the ESI probe tip or gasket).
  • Page 50 To remove the probe adapter: Warning: To avoid personal contamination with biologically hazardous or toxic compounds, wear clean, chemical-resistant, powder-free gloves when performing this procedure. Warning: To avoid harmless, static-like electric shock, ensure that the mass spectrometer is in Standby mode before you touch any of its external surfaces that are marked with this high-voltage warning symbol.
  • Page 51 Maintenance procedures This section provides the maintenance guidelines and procedures necessary to maintain the mass spectrometer's performance. Keep to a maintenance schedule, and perform maintenance as required and described in this section. 4.1 Maintenance Schedule The following table lists periodic maintenance schedules that ensure optimum instrument performance.
  • Page 52 4.2 Spare parts Waters recommends that you replace only the parts mentioned in this document. For spare parts details, see the Waters Quality Parts Locator on the Waters Web site’s Services & Support page (http://www.waters.com/waters/en_US/Spare-Parts/nav.htm?cid=511444). 4.3 Preparing the instrument for working on the source...
  • Page 53 In the Instrument Console system tree, expand Xevo TQ-XS IVD Detector, Interactive Fluidics. Click Control Select Waste as the flow state. In the Instrument Console, click Standby and confirm that the Operate indicator is not illuminated. Wait three minutes to allow the desolvation gas flow to cool the probe and source.
  • Page 54 Warning: To avoid puncture wounds, handle the probe with care. Pull the source enclosure release (located on the bottom, right-hand side) outward and swing open the enclosure. Use both hands to grasp the source enclosure and lift it vertically off the two supporting studs on the source adapter housing.
  • Page 55 To fit the source enclosure to the instrument: Warning: To avoid personal contamination with biologically hazardous, toxic, or corrosive materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when performing this procedure. Use both hands to fit the source enclosure to the two supporting studs on the source adapter housing.
  • Page 56 Warning: To avoid puncture wounds, handle the probe with care. Pull the source enclosure release (located on the bottom, right-hand side) outward and swing open the enclosure. Close the source isolation valve by turning its handle counterclockwise to the vertical position.
  • Page 57 Figure 4–3: Source isolation valve opened Isolation valve handle in open position Close the source enclosure. 4.6 Removing O-rings and seals You must remove O-rings or seals from instrument components when performing certain maintenance procedures. An O-ring removal kit is provided with the instrument. Figure 4–4: O-ring removal kit Tool 1 Tool 2...
  • Page 58 Use the tools as aids to pull the O-ring or seal from its groove. Tip: If you do not plan to reuse the O-ring or seal, you can use the forked end of tool 1 to impale the O-ring or seal to remove it. Warning: To avoid spreading contamination with biologically hazardous, toxic, and corrosive materials, dispose of all waste materials according to local environmental regulations.
  • Page 59 To laboratory exhaust port From instrument pilot valve port Bottle support Nitrogen exhaust trap bottle From instrument exhaust connection One-way valve Required materials Chemical-resistant, powder-free gloves To empty the nitrogen exhaust trap bottle: In the instrument console, click Stop Flow Pull the source enclosure release (located at the bottom, right-hand side) outward, and swing open the enclosure.
  • Page 60 4.9 Maintaining the EBARA oil-free roughing pump There are no user maintenance procedures for the EBARA EV-SA30 pump. To replace the pump, contact Waters Technical Support. For more information, see Contacting Waters. 4.10 Cleaning the source components Clean the sample cone and cone gas nozzle when these conditions apply: •...
  • Page 61 Warning: To avoid puncture wounds, take great care while working with the source enclosure open if an ESI probe is fitted; the ESI probe tip is sharp. Warning: To avoid burn injuries, take great care while working with the source enclosure open.
  • Page 62 Notice: To avoid damage, do not open the source isolation valve before fitting the sampling cone assembly to the ion block assembly. 4.11.2 Disassembling the sampling cone assembly Required materials • Chemical-resistant, powder-free gloves • Combined 2.5-mm hex wrench and cone extraction tool To disassemble the sampling cone assembly: Warning: To avoid personal contamination with biologically hazardous, toxic, or corrosive...
  • Page 63 Figure 4–9: Cone extraction tool Collar Insert the collar in the sample cone. Figure 4–10: Inserting the cone extraction tool Insert the collar Notice: To avoid damaging the sampling cone, which is fragile, do not place it on its tip. Always place it on its flanged base. Rotate and lift the tool and collar to remove the sample cone from the cone gas nozzle.
  • Page 64 Rotate the tool Remove the sample cone Remove the O-ring from the sample cone. Figure 4–12: O-ring removed from the sample cone Cone gas nozzle Sample cone O-ring Cone gas nozzle handle Warning: To avoid spreading contamination with biologically hazardous, toxic, and corrosive materials, dispose of all waste materials according to local environmental regulations.
  • Page 65 • HPLC-grade (or better) water • Formic acid • Ultrasonic bath • Oil-free argon gas or Oil-free nitrogen gas for drying (air-drying optional) • Wash bottle containing HPLC-grade (or better) 1:1 methanol/water • Large beaker To clean the sample cone and cone gas nozzle: Warning: To avoid personal contamination with biologically hazardous, toxic, or corrosive materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean,...
  • Page 66 Carefully remove the components from the vessels and blow dry them with inert, oil-free gas. Inspect each component for persisting contamination. If contamination is present, do as follows: Use the wash bottle containing 1:1 methanol/water to rinse the component over the large beaker.
  • Page 67 O-ring Sample cone Cone gas nozzle Cone gas nozzle handle Note: The PEEK handle is removable on earlier models of the cone gas nozzle assembly. If you detached the handle from the cone gas nozzle for cleaning, replace the handle and tighten it.
  • Page 68 Figure 4–14: Fitting the sampling cone assembly Ion block assembly Sampling cone assembly Grasp the cone gas nozzle handle and use it to rotate the sampling cone assembly 90 degrees, moving the handle downward from the horizontal to the vertical position. Open the source isolation valve (see Opening the source isolation valve).
  • Page 69 To remove the ion block assembly: Warning: To avoid personal contamination with biologically hazardous, toxic, or corrosive materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when performing this procedure. Vent and power-down the mass spectrometer (see the mass spectrometer’s online Help for details).
  • Page 70 Ion block assembly securing screws Remove the ion block assembly from the PEEK ion block support. Figure 4–16: Removing the ion block assembly PEEK ion block support Ion block assembly 4.12.2 Disassembling the source ion block assembly Required materials • Chemical-resistant, powder-free gloves •...
  • Page 71 Figure 4–17: Source ion block assembly Source isolation valve handle in closed position Sampling cone assembly retaining blocks Cone gas nozzle handle Grasp the cone gas nozzle handle, and use it to rotate the sampling cone assembly through 90 degrees. Slide the sampling cone assembly out of the ion block assembly.
  • Page 72 Figure 4–18: Source ion block cover plate Ion block cover plate securing screw Ion block cover plate Remove the ion block cover plate. Grasp the isolation valve, and pull it out of the ion block. Figure 4–19: Removing the isolation valve from the ion block Isolation valve O-ring Use the O-ring removal kit to carefully remove the isolation valve O-ring (see...
  • Page 73 Warning: To avoid spreading contamination with biologically hazardous, toxic, and corrosive materials, dispose of all waste materials according to local environmental regulations. If the isolation valve O-ring shows signs of deterioration or damage, dispose of it in accordance with local environmental regulations. Use the combined 2.5-mm hex wrench and cone extraction tool to loosen the captive PEEK terminal block securing screw.
  • Page 74 Figure 4–21: Removing the PEEK terminal block and ceramic heater mounting block Ceramic heater mounting block PEEK terminal block 11. Use the O-ring removal kit to carefully remove the cover seal from the ion block (see also Removing O-rings and seals).
  • Page 75 Warning: To avoid spreading contamination with biologically hazardous, toxic, and corrosive materials, dispose of all waste materials according to local environmental regulations. 13. If the cover seal or cone gas O-ring shows signs of deterioration or damage, dispose of it in accordance with local environmental regulations.
  • Page 76 Rinse the components by immersing them separately in glass vessels containing water, and then placing the vessels in the ultrasonic bath for 20 minutes. Remove residual water from the components by immersing them in separate glass vessels containing methanol, and then placing the vessels in the ultrasonic bath for 10 minutes.
  • Page 77 Notice: To avoid damaging the heater cartridge assembly wires, do not bend or twist them when removing the assembly and ceramic heater mounting block from the ion block. Carefully fit the PEEK terminal block and ceramic heater mounting block, complete with heater cartridge assembly, to the ion block.
  • Page 78 To fit the ion block assembly to the source assembly: Warning: To avoid personal contamination with biologically hazardous, toxic, and corrosive materials, wear chemical-resistant, powder-free gloves when performing this procedure. Warning: To avoid puncture wounds, take great care while working with the source enclosure open if an ESI probe is fitted;...
  • Page 79 4.13.2 Removing the ion block support from the source assembly Required materials • Chemical-resistant, powder-free gloves • 3-mm hex wrench • O-ring removal kit To remove the ion block support from the source assembly: Warning: To avoid personal contamination with biologically hazardous, toxic, and corrosive materials, wear chemical-resistant, powder-free gloves when performing this procedure.
  • Page 80 Housing Securing screws StepWave assembly Securing screws PEEK ion block support Remove the PEEK ion block support from the adapter housing. Use the O-ring removal kit to carefully remove all the O-rings from the PEEK ion block support (see Removing o-rings and seals).
  • Page 81 Position the seal breaker and locator tool so that it engages as follows: • Its pin with the hole on the adaptor housing directly under the end of the StepWave ion guide assembly. • Its lip behind the StepWave ion guide assembly’s ion guide cap. Figure 4–24: Seal breaker and locator tool: Handle September 11, 2019, 715004990IVD Rev.
  • Page 82 Figure 4–25: Seal breaker and locator tool positioned on the adaptor housing: Adaptor housing Ion guide cap Seal breaker and locator tool Push firmly on the seal breaker and locator tool’s handle, to lever the StepWave assembly slightly out of the adaptor housing. Rationale: Moving the assembly in this manner releases it from a seal located inside the instrument.
  • Page 83 Figure 4–26: StepWave assembly removal and insertion tool Ion block support screw holes (2) Brown PEEK ion guide cap Slot Cutout Pins Notice: To avoid damage when removing the StepWave ion guide assembly from the adapter housing, handle only the brown PEEK ion guide cap. Inserting your thumbs through the slots in the StepWave removal and insertion tool, pull the StepWave ion guide assembly from the pumping block assembly and into the StepWave removal and insertion tool.
  • Page 84 Using both hands, fit the source enclosure to the two supporting studs on the source adaptor housing. Close the source enclosure. Rationale: Fitting and closing the source enclosure prevents debris entering the instrument while you are working on the StepWave ion guide assembly. 4.13.4 Disassembling the StepWave ion guide assembly Warning: To avoid personal contamination with biologically hazardous, toxic, and corrosive...
  • Page 85 Second ion guide assembly Securing screw First ion guide assembly Separate the first and second ion guide assemblies. Figure 4–28: StepWave ion guide assembly separated Second ion guide assembly First ion guide assembly Remove the brown PEEK gasket from the second ion guide assembly. Figure 4–29: StepWave second ion guide assembly September 11, 2019, 715004990IVD Rev.
  • Page 86 Second ion guide assembly Brown PEEK gasket Remove the O-ring from the differential pumping aperture on the second ion guide assembly (see Removing O-rings and seals). Figure 4–30: StepWave second ion guide assembly Differential pumping aperture O-ring Warning: To avoid spreading contamination with biologically hazardous, toxic, and corrosive materials, dispose of all waste materials according to local environmental regulations.
  • Page 87 • Two lengths of PEEK, PTFE, or stainless steel tubing, appropriately sized for suspending the first ion guide and second ion guide assemblies in the glass vessels when cleaning. • HPLC-grade deionized water • Waters MS Cleaning Solution (186006846) • Waste container • HPLC-grade isopropyl alcohol •...
  • Page 88 First ion guide PCB assembly Hook Add Waters MS Cleaning Solution to the glass vessel until the first ion guide PCB assembly is immersed completely. Repeat step 1 through step 3 for the second ion guide PCB assembly, placing the hook through one of the support rod holes.
  • Page 89 Carefully pour the cleaning solution from the vessel holding the first ion guide PCB assembly into the holding container, retaining the ion guide in the vessel. Tip: You can reuse the cleaning solution for one subsequent cleaning. Fill the vessel with deionized water, to rinse the first ion guide PCB assembly, and then discard the water.
  • Page 90 To assemble the StepWave ion guide assembly: Notice: To avoid damaging the StepWave ion guide assembly, handle it and its components carefully throughout the cleaning procedure. In particular, to avoid damaging the wiring on assemblies fitted with an externally wired printed circuit board (PCB), do not touch the wiring.
  • Page 91 Figure 4–34: Fitting the brown PEEK gasket Second ion guide assembly Brown PEEK gasket Align the first ion guide assembly with the second ion guide assembly. Use the combined 2.5-mm hex wrench and cone extraction tool to fit and tighten the two screws securing the first ion guide assembly to the second ion guide assembly.
  • Page 92 Disconnect the probe adjuster and options cables from the instrument's connectors. Pull the source enclosure release outward (located at the bottom, right-hand side) and swing open the enclosure. Using both hands, grasp the source enclosure and lift it vertically off the two supporting studs on the source adapter housing.
  • Page 93 Notice: To avoid damage when removing the StepWave ion guide assembly from the adapter housing, handle only the brown PEEK ion guide cap. Inserting your thumbs through the slots in the StepWave removal and insertion tool, firmly push the StepWave ion guide assembly into the pumping block assembly. Tip: You will detect some resistance to motion when the StepWave assembly encounters the seal inside the instrument.
  • Page 94 Rationale: This fully locates the StepWave assembly in the adapter housing. 10. Remove the seal breaker and locator tool. 4.13.8 Fitting the ion block support to the source Required materials • Chemical-resistant, powder-free gloves • 3-mm hex wrench • New seals and O-rings To fit the PEEK ion block support to the source: Warning: To avoid personal contamination with biologically hazardous, toxic, and corrosive...
  • Page 95 4.14.1 Removing the probe assembly Required materials • Chemical-resistant, powder-free gloves To remove the probe assembly: Warning: To avoid personal contamination with biologically hazardous, toxic, and corrosive materials, wear chemical-resistant, powder-free gloves when performing this procedure. Warning: To avoid puncture wounds, handle sharp parts and materials with care. If the probe assembly is fitted, disconnect it from the inlet system.
  • Page 96 Unscrew the finger-tight PEEK fitting. Figure 4–38: Probe assembly PEEK fitting PEEK fitting Remove the probe assembly. Warning: To avoid spreading contamination with biologically hazardous, toxic, and corrosive materials, dispose of all waste materials according to local environmental regulations. Dispose of the probe assembly in accordance with local environmental regulations. To install a new probe assembly, see Installing the probe assembly.
  • Page 97 To remove the ESI probe tip and gasket: Warning: To avoid personal contamination with biologically hazardous materials, wear clean, chemical-resistant, powder-free gloves when performing this procedure. Warning: To avoid burn injuries, take great care while performing this procedure. Warning: To avoid puncture injuries, handle sample needles, syringes, fused silica lines, and borosilicate tips with extreme care.
  • Page 98 Figure 4–40: Removing the metal gasket Metal gasket Warning: To avoid spreading contamination with biologically hazardous, toxic, and corrosive materials, dispose of all waste materials according to local environmental regulations. Dispose of the metal gasket in accordance with local environmental regulations. If the probe tip is damaged, dispose of it in accordance with local environmental regulations.
  • Page 99 Fit the new metal gasket into the probe tip. Figure 4–41: Inserting the gasket Metal gasket ESI probe tip Stainless steel tube Fit the probe tip and screw the tip onto the probe assembly. Use the 7-mm wrench in conjunction with the 10-mm wrench to tighten the probe tip. Important: To avoid gas leakage, fully tighten the probe tip.
  • Page 100 Remove the ion block assembly from the instrument (see Removing the ion block assembly from the source assembly). Ensure that the isolation valve is closed. Figure 4–42: Source isolation valve closed Source isolation valve handle in closed position Sampling cone assembly retaining blocks Cone gas nozzle handle Use the combined 2.5-mm hex wrench and cone extraction tool to loosen the two captive screws securing the ion block cover plate.
  • Page 101 Remove the ion block cover plate. Use the combined 2.5-mm hex wrench and cone extraction tool to loosen the captive PEEK terminal block securing screw. Figure 4–44: PEEK terminal block securing screws Heater cartridge assembly wires PEEK terminal block securing screw Notice: To avoid damaging the heater cartridge assembly wires, do not bend or twist them when removing the assembly and ceramic heater mounting block from...
  • Page 102 Heater wire securing screws PEEK terminal block Ceramic heater mounting block Use the combined 2.5-mm hex wrench and cone extraction tool to loosen the two screws securing the heater wires to the PEEK terminal block. Disconnect the heater cartridge wires from the PEEK terminal block. Use the needle-nose pliers to gently grasp the heat-shrink tubing on the heater cartridge assembly, and then slide the assembly out of the ceramic heater mounting block.
  • Page 103 15. Use the combined 2.5-mm hex wrench and cone extraction tool to tighten the captive PEEK terminal block securing screw. 16. Fit the ion block cover plate to the ion block assembly, and then use the combined 2.5-mm hex wrench and cone extraction tool to tighten the two captive screws securing the ion block cover plate.
  • Page 104 Remove the source enclosure from the instrument (see Removing the source enclosure from the instrument). Use the O-ring removal kit to carefully remove the following seals from the probe adjuster assembly: • Probe seal • Nebulizer gas seal See also: Removing O-rings and seals.
  • Page 105 Figure 4–48: Source enclosure seals Nebulizer gas seal Desolvation gas seal Source enclosure seal Warning: To avoid spreading contamination with biologically hazardous, toxic, and corrosive materials, dispose of all waste materials according to local environmental regulations. Dispose of all the seals in accordance with local environmental regulations. 4.17.2 Fitting the new source enclosure and probe adjuster assembly probe seals Required materials...
  • Page 106 To fit the new source enclosure and probe adjuster assembly probe seals: Warning: To avoid personal contamination with biologically hazardous, toxic, or corrosive materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when performing this procedure. Ensure that all the grooves for seals are free from dirt and debris.
  • Page 107 4.18 Replacing the air filter inside the front door Required materials • Needle-nose pliers • New filter To replace the air filter inside the front door: Open the access door to the fluidics pump. Unscrew the captive thumbscrew on the filter cover. Figure 4–50: Unscrewing the captive thumbscrew Thumbscrew Filter cover...
  • Page 108 Filter Filter cover Lift the filter, vertically, from its slot in the instrument. Tip: If necessary, use the needle-nose pliers to grasp the filter. Figure 4–52: Removing the filter Dispose of the filter. Fit the new filter into the instrument. Fit the filter cover to the instrument.
  • Page 109 Heed all warnings when you install, repair, or operate any Waters instrument or device. Waters accepts no liability in cases of injury or property damage resulting from the failure of individuals to comply with any safety precaution when installing, repairing, or operating any of its instruments or devices.
  • Page 110 (Risk of high-pressure gas release.) A.1.1 Specific warnings A.1.1.1 Burst warning This warning applies to Waters instruments and devices fitted with nonmetallic tubing. Warning: To avoid injury from bursting, nonmetallic tubing, heed these precautions when working in the vicinity of such tubing when it is pressurized: •...
  • Page 111 LC solvent should the nitrogen supply fail. A.1.1.4 Biohazard warning The following warning applies to Waters instruments and devices that can process biologically hazardous materials. Biologically hazardous materials are substances that contain biological agents capable of producing harmful effects in humans.
  • Page 112 Warning: To avoid personal contamination with biologically hazardous, toxic, or corrosive materials, you must understand the hazards associated with their handling. Guidelines prescribing the proper use and handling of such materials appear in the latest edition of the National Research Council's publication, Prudent Practices in the Laboratory: Handling and Management of Chemical Hazards.
  • Page 113 A.5 Warnings that apply to all Waters instruments and devices When operating this device, follow standard quality-control procedures and the equipment guidelines in this section. Warning: Changes or modifications to this unit not expressly approved by the party responsible for compliance could void the user’s authority to operate the equipment.
  • Page 114 Warning: Use caution when working with any polymer tubing under pressure: • Always wear eye protection when near pressurized polymer tubing. • Extinguish all nearby flames. • Do not use tubing that has been severely stressed or kinked. • Do not use nonmetallic tubing with tetrahydrofuran (THF) or concentrated nitric or sulfuric acids.
  • Page 115 Avvertenza: fare attenzione quando si utilizzano tubi in materiale polimerico sotto pressione: • Indossare sempre occhiali da lavoro protettivi nei pressi di tubi di polimero pressurizzati. • Spegnere tutte le fiamme vive nell'ambiente circostante. • Non utilizzare tubi eccessivamente logorati o piegati. •...
  • Page 116 • 非金属チューブには、テトラヒドロフラン(THF)や高濃度の硝酸または硫酸などを流さないでくださ い。 • 塩化メチレンやジメチルスルホキシドは、非金属チューブの膨張を引き起こす場合があり、その場 合、チューブは極めて低い圧力で破裂します。 This warning applies to Waters instruments fitted with nonmetallic tubing or operated with flammable solvents. Warning: The user shall be made aware that if the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired.
  • Page 117 警告： 使用者必須非常清楚如果設備不是按照製造廠商指定的方式使用，那麼該設備所 提供的保護將被消弱。 경고: 제조업체가 명시하지 않은 방식으로 장비를 사용할 경우 장비가 제공하는 보호 수단이 제대로 작동하지 않을 수 있다는 점을 사용자에게 반드시 인식시켜야 합니다. 警告: ユーザーは、製造元により指定されていない方法で機器を使用すると、機器が提供している 保証が無効になる可能性があることに注意して下さい。 A.6 Warnings that address the replacement of fuses The following warnings pertain to instruments and devices equipped with user-replaceable fuses. Information describing fuse types and ratings sometimes, but not always, appears on the instrument or device.
  • Page 118 警告: 火災予防のために、ヒューズ交換では機器ヒューズカバー脇のパネルに記載されているタイプお よび定格のヒューズをご使用ください。 Finding fuse types and ratings when that information does not appear on the instrument or device: Warning: To protect against fire, replace fuses with those of the type and rating indicated in the “Replacing fuses” section of the Maintenance Procedures chapter. Avertissement : pour éviter tout risque d'incendie, remplacez toujours les fusibles par d'autres du type et de la puissance indiqués dans la rubrique "Remplacement des...
  • Page 119 Symbol Description Electrical power off Standby Direct current Alternating current Alternating current (three phase) Safety ground Frame or chassis terminal connection Fuse Functional ground Input Output Indicates that the device or assembly is susceptible to damage from electrostatic discharge (ESD) A.8 Handling symbols The following handling symbols and their associated statements can appear on labels affixed to the packaging in which instruments, devices, and component parts are shipped.
  • Page 120 Symbol Description Fragile! Use no hooks! Upper limit of temperature Lower limit of temperature Temperature limitation September 11, 2019, 715004990IVD Rev. A Page 120...
  • Page 121 To avoid damaging the mass spectrometer, observe the following precautions: • Contact Waters Technical Service before moving the instrument. • If you must transport the instrument, or remove it from service, contact Waters Technical Service for recommended cleaning, flushing, and packaging procedures.
  • Page 122 Shielded Ethernet Video camera connection (not used) Event inputs and outputs Power Roughing pump connectors Roughing pump grounding connnection Nitrogen exhaust Nitrogen inlet Pilot valve port Turbo vacuum Source vacuum Collision cell gas inlet B.2 Connecting the EBARA roughing pump For proper ventilation, the following minimum clearances must apply: Figure B–2: EBARA oil-free roughing pump clearances September 11, 2019, 715004990IVD Rev.
  • Page 123 Right side minimum clearance is 50 mm (2 inches) Top minimum clearance is 100 mm (4 inches) Front minimum clearance is 100 mm (4 inches) Left side minimum clearance is 50 mm (2 inches) Rear minimum clearance is 100 mm (4 inches) Required materials •...
  • Page 124 Use elbow connectors for fitting the vacuum and exhaust tubing to the pump to reduce the height so it fits under a workbench. Figure B–3: Roughing pump tubing connections to rear of the Xevo TQ-XS IVD mass spectrometer NW40/NW25 reducer...
  • Page 125 Figure B–4: Roughing pump tubing connections to the EBARA pump NW40 clamp Vacuum hose (from the rear of the Xevo TQ-XS IVD) Exhaust tubing NW40 clamp Exhaust silencer NW40 clamp and exhaust filter (supplied with the EBARA roughing pump installation...
  • Page 126 B.3 Making the electrical connections to the Ebara oil-free roughing pump Figure B–5: Roughing pump electrical connections to rear of the Xevo TQ-XS IVD mass spectrometer Pump override switch (for use by Waters engineers only) Electronics ON/OFF switch (for use by Waters engineers only) Backing pump control connector September 11, 2019, 715004990IVD Rev.
  • Page 127 B.4 Connecting to the nitrogen gas supply Required materials • Chemical-resistant, powder-free gloves • 6-mm PTFE tubing (included in the Xevo TQ-XS IVD Installation Kit) • Nitrogen regulator (not supplied) To connect the nitrogen gas supply: Connect one free end of the 6-mm PTFE tubing to the nitrogen inlet port on the rear of the instrument.
  • Page 128 Collision cell gas inlet Source vacuum Nitrogen exhaust Nitrogen inlet port Pilot valve port Turbo vacuum Attach a nitrogen regulator to the nitrogen supply. Requirement: The nitrogen must be dry and oil-free, with a purity of at least 95%. Connect the free end of the 6-mm PTFE tubing to the nitrogen regulator. Set the nitrogen regulator to 690 kPa (6.9bar, 100 psi).
  • Page 129 • Chemical-resistant, powder-free gloves • Utility knife • Nitrogen exhaust trap bottle • 4-mm PTFE tubing and 12-mm PTFE tubing (included in the Xevo TQ-XS IVD installation kit) To connect the nitrogen exhaust line: Warning: To prevent the escape of exhaust gases into the laboratory, carrying biologically hazardous, toxic, or corrosive LC solvents, you must use a source exhaust line connected to either a laboratory fume hood, or to an active laboratory exhaust system.
  • Page 130 Figure B–7: Nitrogen exhaust trap bottle: To laboratory exhaust port From instrument pilot valve port Bottle support Nitrogen exhaust trap bottle From instrument exhaust connection One-way valve Notice: To avoid gas leaks, use the tube cutter to cut the PTFE tubing squarely. Cut a length of 4-mm tubing long enough to connect the instrument to the nitrogen exhaust trap bottle.
  • Page 131 Connect one end of the tubing to the exhaust port on the instrument’s rear panel. Connect the free end of the tubing to the inlet port on the nitrogen exhaust trap bottle. Notice: To avoid gas leaks, use the tube cutter to cut the PTFE tubing squarely. Cut a second length of 12-mm tubing long enough to connect the nitrogen exhaust trap bottle to the exhaust vent.
  • Page 132 Figure B–8: Connecting liquid waste lines Drain cup Barbed fitting Drain line Warning: To prevent leakage of biologically hazardous, toxic, or corrosive materials: • Do not crimp or bend drain lines. A crimp or bend can impede flow to the waste container.
  • Page 133 Correct Incorrect B.8 Connecting the workstation (systems with no ACQUITY Before connecting the workstation to the instrument, set up the workstation according to its accompanying instructions. Locate the workstation within three meters (ten feet) of the mass spectrometer. Requirement: Use shielded network cables with the mass spectrometer to ensure compliance with FCC limits.
  • Page 134 B.9 Connecting Ethernet cables (systems with ACQUITY LC) Requirement: Use shielded network cables with the mass spectrometer to ensure compliance with FCC limits. To make Ethernet connections: Connect one end of one shielded Ethernet cable to the ACQUITY instrument’s network switch, and then connect the free end to the Ethernet card on the preconfigured ACQUITY workstation.
  • Page 135 Figure B–10: Input and output connector locations LA N External C onnections 1 Service Bus EPC Com Port External C onnections2 Video Output OUT - External Connections 1 External Connections 2 System Activity External connections identification tables External connections 1 External connections 2 Figure B–11: Input/output signal connector configuration Table B–1: External connections 1:...
  • Page 136 HAR-type (or better) cords in Europe. The power cords must be replaced only with ones of adequate rating. For information regarding which cord to use in other countries, contact your local Waters distributor. September 11, 2019, 715004990IVD Rev. A Page 136...
  • Page 137 Connect the female end of the power cord to the receptacle on the rear panel of the mass spectrometer. Connect the male end of the mass spectrometer power cord to a suitable 200 to 240 V AC wall outlet. September 11, 2019, 715004990IVD Rev. A Page 137...
  • Page 138 C.1 Preventing contamination For information on preventing contamination, refer to Controlling Contamination in LC/MS Systems (715001307). You can find this document on www.waters.com by clicking Services & Support > Support Library > Support Library. C.2 Items exposed to solvent The items that appear in the following table can be exposed to solvent.
  • Page 139 Table C–1: Items exposed to solvent: (continued) Item Material Probe adjuster bellows Viton Probe shaft PEEK Pumping block Aluminium and Xylan Solvent waste/leak management Tygon tubing 2375, polyurethane Source enclosure Aluminium Source enclosure view port Silica float glass Trap bottle Polypropylene Trap bottle push-in fittings Nitrile butadiene rubber, stainless steel,...
  • Page 140 Strong solvents used to prepare mobile phases for normal-phase LC/MS (for example, hexane or tetrahydrofuran (THF)) will adversely affect the performance of the materials shown in the table Items exposed to solvent and must not be used. You must evaluate the safety issues if these solvents are used as additives to the mobile phases at reduced concentration, or as sample diluents.
  • Page 141 Support > Support Library > Support Library. D.2 Plumbing schematic For further information, refer to the diagram on the inside of the fluidics valve access door (see ACQUITY UPLC I-Class/Xevo TQ-XS IVD system). Requirement: Ensure that the end of the tubing is fully submerged in the solvent in the wash reservoir.
  • Page 142 Selector valve Diverter valve ACQUITY system Source Waste Wash Vial C Vial B Vial A Analyte pump D.3 Tubing specifications The following table gives the internal diameter (ID), external diameter (ED), color, length, and quantity for the IntelliStart Fluidics tubing. Table D–1: Replacement tubing specifications: Connection ID (inches)