Related Manuals for Waters ThermaBeam Mass
Summary of Contents for Waters ThermaBeam Mass
- Page 1 Waters ThermaBeam Mass Detector Installation and Maintenance Guide 34 Maple Street Milford, MA 01757 71500032004, Revision A...
- Page 2 This document is believed to be complete and accurate at the time of publication. In no event shall Waters Corporation be liable for incidental or consequential damages in connection with, or arising from, the use of this document.
- Page 3 If you observe a change in the retention of a particular compound, in the resolution between two compounds, or in peak shape, immediately determine the reason for the changes. Until you determine the cause of a change, do not rely on the separation results. Note: The Installation Category (Overvoltage Category) for this instrument is Level II.
- Page 4 Caution: Use caution when working with any polymer tubing under pressure: • Always wear eye protection when near pressurized polymer tubing. • Extinguish all nearby flames. • Do not use Tefzel tubing that has been severely stressed or kinked. • Do not use Tefzel tubing with tetrahydrofuran (THF) or concentrated nitric or sulfuric acids.
- Page 5 Precauzione: prestare attenzione durante le operazioni con i tubi di polimero sotto pressione: • Indossare sempre occhiali da lavoro protettivi nei pressi di tubi di polimero pressurizzati. • Estinguere ogni fonte di ignizione circostante. • Non utilizzare tubi Tefzel soggetti a sollecitazioni eccessive o incurvati. •...
- Page 7 Caution: The user shall be made aware that if the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired. Attention : L’utilisateur doit être informé que si le matériel est utilisé d’une façon non spécifiée par le fabricant, la protection assurée par le matériel risque d’être défectueuses.
- Page 8 Caution: To protect against fire hazard, replace fuses with those of the same type and rating. Attention : Remplacez toujours les fusibles par d’autres du même type et de la même puissance afin d’éviter tout risque d’incendie. Vorsicht: Zum Schutz gegen Feuergefahr die Sicherungen nur mit Sicherungen des gleichen Typs und Nennwertes ersetzen.
- Page 9 Caution: To avoid possible electrical shock, power off the instrument and disconnect the power cord before servicing the instrument. Attention : Afin d’éviter toute possibilité de commotion électrique, mettez hors tension l’instrument et débranchez le cordon d’alimentation de la prise avant d’effectuer la maintenance de l’instrument.
- Page 10 Commonly Used Symbols Direct current Courant continu Gleichstrom Corrente continua Corriente continua Alternating current Courant alternatif Wechselstrom Corrente alternata Corriente alterna Protective conductor terminal Borne du conducteur de protection Schutzleiteranschluss Terminale di conduttore con protezione Borne del conductor de tierra...
- Page 11 Commonly Used Symbols (Continued) Frame or chassis terminal Borne du cadre ou du châssis Rahmen- oder Chassisanschluss Terminale di struttura o telaio Borne de la estructura o del chasis Caution or refer to manual Attention ou reportez-vous au guide Vorsicht, oder lesen Sie das Handbuch Prestare attenzione o fare riferimento alla guida Actúe con precaución o consulte la guía Caution, hot surface or high temperature...
- Page 12 Commonly Used Symbols (Continued) Caution, risk of electric shock (high voltage) Attention, risque de commotion électrique (haute tension) Vorsicht, Elektroschockgefahr (Hochspannung) Precauzione, rischio di scossa elettrica (alta tensione) Precaución, peligro de descarga eléctrica (alta tensión) Caution, risk of needle-stick puncture Attention, risques de perforation de la taille d’une aiguille Vorsicht, Gefahr einer Spritzenpunktierung Precauzione, rischio di puntura con ago...
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Page 13: Intended Use
ThermaBeam Mass Detector Information Intended Use ® ™ The Waters ThermaBeam Mass Detector can be used for forensic and metabolic diagnostic testing to analyze many compounds, including diagnostic indicators and therapeutically monitored compounds. When you develop methods, follow the “Protocol for the Adoption of Analytical Methods in the Clinical Chemistry Laboratory,”... -
Page 14: Table Of Contents
1.3.6 Cold Trap (Optional)........... 55 1.3.7 Isolation Valves ............56 1.3.8 Exhaust Trap Bucket ..........58 Chapter 2 Control Systems in the ThermaBeam Mass Detector ..... 59 2.1 Overview ................59 2.2 LC Flow Rate Considerations ..........63 Table of Contents... - Page 15 2.5.5 Cold Cathode Vacuum Gauge ........72 2.5.6 Vacuum Interlock Gauge ........... 73 2.6 MS Software Control ............. 73 2.6.1 Tuning the ThermaBeam Mass Detector ....74 2.6.2 Optimizing the ThermaBeam Interface ...... 75 2.6.3 Acquiring and Processing Data ......... 75 2.6.4 Reporting MS Data ............
- Page 16 3.4.6 Connecting the Oil Mist Filters........95 3.4.7 Connecting the Gas Ballast/Oil Return...... 96 3.4.8 Connecting the Exhaust Hoses ......... 99 3.4.9 Connecting the Exhaust Trap Bucket ....... 101 3.4.10 Connecting the Vacuum Hoses ......102 3.4.11 Installing the Cold Trap (No Longer Available)..106 3.5 Handling Mobile Phases .............
- Page 17 Chapter 6 Maintenance .................. 182 6.1 Performing Routine Maintenance........182 6.1.1 Shutting Down the ThermaBeam Mass Detector ..183 6.1.2 Starting Up the ThermaBeam Mass Detector ..184 6.2 Maintaining the Vacuum Pumps.......... 185 6.2.1 Checking the Vacuum Pump Oil ......187...
- Page 18 6.2.2 Changing the Vacuum Pump Oil......187 6.2.3 Emptying the Exhaust Trap Bucket ......190 6.2.4 Cleaning the Pirani Gauge........191 6.2.5 Changing the Oil Mist Filter Elements ..... 193 6.3 Changing the Oil Wick Cartridge ........197 6.4 Defrosting the Optional Cold Trap ........200 6.5 Cleaning LC/MS Interface Components......
- Page 19 Conceptual View of the Integrity System ........34 ThermaBeam Mass Detector (Front View) ........35 ThermaBeam Mass Detector (Rear View)........36 Internal Components of the ThermaBeam Mass Detector .... 37 ThermaBeam LC/MS Interface and Vacuum Chamber Processes ..................38 ThermaBeam LC/MS Interface Components......... 40 Thermal Pneumatic Nebulization...........
- Page 20 ThermaBeam Mass Detector............61 Helium Flowmeter................66 Diverter Valve Assembly ..............70 Pirani Gauge.................. 72 Cold Cathode Vacuum Gauge and Vacuum Interlock Gauge..72 Run Samples ................. 76 Vacuum Pump Configuration ............83 Vacuum System Fittings ..............85 Oil Filler Plugs................86 Filling the Oil ..................
- Page 21 3-30 Connecting the Union to the Nebulizer Bracket ......121 3-31 Diverter Valve Port Connections..........122 3-32 Side Panel of ThermaBeam Mass Detector ........ 123 3-33 Helium Inlet Port ................125 3-34 Connecting the Helium Supply to the Nebulizer ......125 3-35 Helium Flowmeter Knob ..............
- Page 22 4-11 Run Samples ................157 4-12 Manual Tune Window..............158 4-13 TMD System Status Dialog Box ..........159 Service Signal Connector ............161 Chamber Pressure Nomograph ........... 164 Switching Filaments in the Manual Tune Window......169 Verifying Helium Flow in the Manual Tune Window ..... 171 Vacuum System Fittings ..............
- Page 23 6-23 Removing the Ion Volume............212 6-24 Cleaning the Ion Volume.............. 213 6-25 Disconnecting the Ionizer Wiring Harness........214 6-26 Removing the Ionizer..............215 6-27 Cleaning the Ionizer..............216 6-28 Cleaning the Extractor Lens ............217 6-29 Disconnecting the Quadrupole Mass Filter........219 6-30 Removing the Quadrupole Mass Filter ........
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Page 24: List Of Tables
Troubleshooting with the Front Panel Indicator Lights ....145 System Status Information ............ 155 Cold Cathode Vacuum Gauge Calibration Data ......162 Troubleshooting the ThermaBeam Mass Detector ....165 Troubleshooting the Vacuum System ........173 Major Components of Air............176 Troubleshooting IEEE-488 Bus Communications .... - Page 25 Power Specifications ..............233 Communications Specification............ 233 Regulatory Compliance .............. 233 Spare Parts ................. 235 Integrity System Tubing .............. 238 List of Tables...
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Page 26: Preface
Preface The Waters ThermaBeam Mass Detector Installation and Maintenance Guide describes the procedures for unpacking, installing, operating, maintaining, and troubleshooting the ® Waters ThermaBeam™ Mass Detector. It also includes appendixes that list specifications and spare parts. This guide is intended for use by individuals who need to install, operate, maintain, and/or troubleshoot the Waters ThermaBeam Mass Detector. - Page 27 The following documentation is provided with your Integrity System. Integrity System Electronic Documents With the purchase of your Integrity System, Waters supplies an electronic copy of all Integrity System documents on a CD-ROM. To install and view these documents on your computer, refer to the Readme file on the CD-ROM.
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Page 28: Documentation Conventions
Adobe Acrobat Reader lets you easily print pages, page ranges, or the entire document by selecting File > Print. For optimum print quantity, Waters recommends that you specify a ® PostScript printer driver for your printer. Ideally, use a printer that supports 600 dpi print resolution. - Page 29 Convention Usage (Continued) Underlined Blue Indicates hypertext cross-references to a specific chapter, section, subsection, or sidehead. Clicking this topic using the hand symbol brings you to this topic within the document. Right-clicking and selecting Go Back from the shortcut menu returns you to the origi- nating topic.
- Page 30 Cautions Cautions provide information essential to the safety of the operator. For example: Caution: To avoid burns, turn off the lamp at least 30 minutes before removing it for replacement or adjustment. Caution: To avoid electrical shock and injury, turn off the detector and unplug the power cord before performing maintenance procedures.
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Page 31: Chapter 1 Introduction
• ThermaBeam Mass Detector • Vacuum system (with two vacuum pumps and an optional cold trap) • Waters 2690 or 2695 Separations Module (with column heater, seal wash, integrated vacuum degasser, solvent bottle tray, and internal column bypass module (CBM) •... -
Page 32: Configuration Of The Integrity System With Cold Trap (No Longer Available)
996/2996 Detector ThermaBeam Empower Mass Personal Solvent Bottle Detector Workstation Tray 2690/2695 Separations Module with Internal CBM Exhaust Vent Cold Trap Exhaust Trap Stage 1 Stage 2 (Optional) Bucket Vacuum Pump Vacuum Pump Cold Trap Stage 1 Stage 2 Exhaust Trap (Optional) Vacuum Pump Vacuum Pump... -
Page 33: Configuration Of The Integrity System Without Cold Trap
996/2996 PDA Detector Empower ThermaBeam Mass Personal Solvent Bottle Detector Workstation Tray 2690/2965 Separations Module with Internal CBM Exhaust Vent Stage 1 Stage 2 Exhaust Trap Vacuum Pump Bucket Vacuum Pump TP01442A Figure 1-2 Configuration of the Integrity System without Cold Trap Figure 1-3 conceptually describes the sequence and locations of the separating, analyzing, and reporting functions of the Integrity System. -
Page 34: Thermabeam Mass Detector Overview
Data System Reports Figure 1-3 Conceptual View of the Integrity System For more information about the Integrity System, see the Waters Integrity System Getting Started Guide. 1.2 ThermaBeam Mass Detector Overview The ThermaBeam Mass Detector is a quadrupole mass spectrometer specifically designed to interface with an HPLC system using patented particle beam technology. -
Page 35: Thermabeam Mass Detector (Front View)
The ThermaBeam Mass Detector can be used to acquire data for compounds that produce ions with mass-to-charge (m/z) ratios from 10 to 1000. The instrument is tuned and calibrated to detect singly charged positive ions that result from the vaporization and ionization of analytes from an LC eluent stream. -
Page 36: Thermabeam Mass Detector (Rear View)
IEEE-488 DIP Switch IEEE-488 Connector Service Signal Connector User Interface Connector Fuse Helium Receptacle Inlet TP01188 Figure 1-5 ThermaBeam Mass Detector (Rear View) Introduction... -
Page 37: Internal Components Of The Thermabeam Mass Detector
Figure 1-6 shows the internal components of the ThermaBeam Mass Detector. Ionizer Quadrupole Mass Filter Vacuum Chamber Vacuum Chamber Ion Detector Expansion Region Turbomolecular and Momentum Pump Separator Helium Flowmeter Nebulizer Fixed Leak Vent Valve Diverter Valve Opening for Front Vacuum... -
Page 38: Thermabeam Lc/Ms Interface And Vacuum Chamber Processes
5. Mass Sorting 6. Ion Detection Quadrupole Mass Filter Electron Multiplier Filament 4. Ionization Extractor Lens – – Signal – Ion Volume – + + + – Preamp 3. Momentum Separation Momentum Exit Conversion Separator Dynode 2. Desolvation Turbomolecular 1. Nebulization Expansion Region Pump Helium Flow... -
Page 39: Thermabeam Lc/Ms Interface
Rotary-vane vacuum pumps and a turbomolecular pump provide the high vacuum required for the operation of the ThermaBeam Mass Detector. For more information about the vacuum system, see Section 1.3, Vacuum System Overview. 1.2.1 ThermaBeam LC/MS Interface In the ThermaBeam LC/MS interface, the sample undergoes: •... -
Page 40: Thermabeam Lc/Ms Interface Components
Momentum Stage 2 Separator Stage 1 Expansion Region +28 V Interlock Magnet O-Ring +28 V Interlock Magnet Sensor Fused Silica Capillary Orifice Nebulizer Helium Connection Fused Silica Capillary LC Connection TP01195 Figure 1-8 ThermaBeam LC/MS Interface Components As shown in Figure 1-9, in the nebulizer, LC effluent passes through a heated fused silica capillary and emerges as a very fine aerosol in the expansion region. -
Page 41: Thermal Pneumatic Nebulization
Empower MS software. Particles from the nebulizer enter the heated expansion region where desolvation continues. The temperature of the expansion region is also controlled through the Empower MS software. ThermaBeam Mass Detector Overview... -
Page 42: Ionizer
Momentum Separation Two successive vacuum stages in the momentum separator (see Figure 1-8) reduce the pressure, causing further separation of the analyte particles from the vaporized solvent. The pressure reduction that occurs at the apertures between successive vacuum stages forces the aerosol-vapor mixture to expand and accelerates it toward the ionizer. About 90% of the solvent is carried off by the Stage 1 vacuum pump. -
Page 43: Electron Impact Ionization
Figure 1-12. Prequad Filters The prequad filters (Figure 1-12) focus the ions into the aperture of the quadrupole mass filter. They also slow down the ions and eliminate fringe field effects. ThermaBeam Mass Detector Overview... -
Page 44: Quadrupole Mass Filter
Prequad Filters TP01251 Figure 1-12 Prequad Filters 1.2.3 Quadrupole Mass Filter The quadrupole mass filter (Figure 1-13) sorts the ionized molecular fragments produced in the ion volume by their mass-to-charge (m/z) ratio. The quadrupole mass filter consists of two pairs of precisely parallel rods that are controlled by a combination of radio frequency (rf) and direct current (dc) potentials. -
Page 45: Ion Detector
1.3 Vacuum System Overview This section describes the system that provides the high vacuum required for the operation of the ThermaBeam Mass Detector. Vacuum System Overview... - Page 46 The rotary-vane vacuum pumps can be connected to either the front or the rear of the ThermaBeam Mass Detector. For information about the Pirani gauge, see Chapter 2, Control Systems in the ThermaBeam Mass Detector. For information about how to install the vacuum system, see Chapter 3, Installing the ThermaBeam Mass Detector. Introduction...
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Page 47: Rotary-Vane Vacuum Pumps
Cold Cathode Gauge Vacuum Chamber Vacuum Interlock Turbomolecular Pump Fixed Leak Vent Valve Oil Mist To Exhaust Filters Vent Vacuum Hoses Isolation Valves Pirani Gauge Cold Trap Stage 1 Stage 2 Exhaust Trap Vacuum Pump (Optional; Vacuum Pump Bucket TP01436A no longer available) Figure 1-15 Vacuum System Configuration with Optional Cold Trap (No Longer Available) -
Page 48: Rotary-Vane Vacuum Pump (Top And Left-Side View)
Inlet Outlet Oil Filler Plugs Power Switch Gas Ballast Drain Plug Control Port Mode Selector Control Sight Glass TP01309 Figure 1-16 Rotary-Vane Vacuum Pump (Top and Left-Side View) The right side of the rotary-vane vacuum pump is shown in Figure 1-17. - Page 49 change the voltage settings for the vacuum pumps, see Section 3.4.3, Checking the Vacuum Pump Voltage Setting. Mode Selection The Rotary-Vane vacuum pumps have a mode selector control knob (see Figure 1-16) that can be used to select a high-throughput mode or a high-vacuum mode. Set both vacuum pumps to run in the high-throughput mode (turn the mode selector control knob fully counterclockwise).
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Page 50: Oil Mist Filters And Gas Ballast/Oil Returns
• Use only pump oil approved by Waters. Refer to Appendix B for the part number. • Check the level of the oil and its appearance daily. The condition of the oil can change quickly and the performance and life of the pump can deteriorate rapidly if the oil is not maintained properly. -
Page 51: Turbomolecular Pump
1.3.3 Turbomolecular Pump An internal, high-vacuum turbomolecular pump (Figure 1-19) maintains the necessary vacuum in the vacuum chamber. Attention: To prevent damage to the turbomolecular pump, avoid mechanical shock STOP to the ThermaBeam Mass Detector during operation. Vacuum System Overview... -
Page 52: Turbomolecular Pump
Turbomolecular Pump TP01326 Figure 1-19 Turbomolecular Pump The turbomolecular pump removes from the vacuum chamber: • Nonionized solvent • Nonionized analytes • Ionization by-products Vacuum Chamber The vacuum chamber houses the ionizer, the quadrupole mass filter, and the ion detector (Figure 1-20). -
Page 53: Fixed Leak Assembly
Pump Figure 1-20 View of the Vacuum Chamber Components 1.3.4 Fixed Leak Assembly The ThermaBeam Mass Detector has been designed with a fixed leak to prevent oil and solvent contaminants from entering the vacuum chamber. Background All oil-sealed mechanical vacuum pumps backstream oil to some extent. As the pressure falls and the oil becomes hot from the operation of the pump, oil vapors develop and work their way back up the vacuum tubing and into the vacuum chamber. -
Page 54: Vent Valve
Description The fixed leak is a stainless steel coiled tube and filter that allows a small amount of air to flow into the Stage 1 pumping line. This helps to prevent oil from backstreaming from the Stage 1 pump (Figure 1-21). -
Page 55: Cold Trap (Optional)
Stage 2 pump and is controlled by the ac power supply. Vent time is approximately 2 minutes. 1.3.6 Cold Trap (Optional) Note: Waters no longer offers the cold trap. The cold trap information in this guide is for information only, to support existing installations. The optional cold trap (Figure... -
Page 56: Isolation Valves
Isolation Valves Flange Seal Power Switch Temperature Indicator Light Drain Vacuum Port Knob TP01253 Drain Outlet Figure 1-23 Cold Trap (No Longer Available) The cold trap, therefore, helps to increase the time between oil changes and prevents damage to the Stage 1 vacuum pump. The cold trap does not collect highly volatile solvents, such as methanol, which also pass easily through the pump. -
Page 57: Isolation Valve On Vacuum Pump
Isolation Valve TP01185 Figure 1-24 Isolation Valve on Vacuum Pump The isolation valves are useful when you want to do maintenance on parts of the system without having to power off the vacuum pumps. Note: Do not power off the vacuum pumps unless you are doing maintenance on the pumps. -
Page 58: Exhaust Trap Bucket
1.3.8 Exhaust Trap Bucket The exhaust trap bucket (Figure 1-25) captures condensed solvent vapors and oil from the two vacuum pumps. This prevents solvent and oil from plugging the exhaust hoses. It is important to position the exhaust hoses so that condensation flows down into the bucket. -
Page 59: Control Systems In The Thermabeam Mass Detector
4500 psi (31.43 mPa). Tuning You need to check the tuning and calibration of the ThermaBeam Mass Detector daily to ensure the accuracy and reproducibility of your data. For procedures on how to tune the TMD, refer to Chapter 2 of the Waters Integrity System Getting Starting Guide. -
Page 60: Mobile Phase Compounds Compatible With The Integrity System
Compound Name Solvents Acetone Acetonitrile Ethanol Ethylacetate Isopropanol Methanol Tetrahydrofuran (THF) Water Acids Acetic Butyric Carbonic Formic Trichloroacetic acid (TCA) Trifluoroacetic acid (TFA) Bases Acetate Ammonium hydroxide Bicarbonate Carbonate Dibutylamine Diethylamine Formate Propionate Triethylamine Control Systems in the ThermaBeam Mass Detector... -
Page 61: Thermabeam Mass Detector
LC/MS Interface Operating Parameters Figure 2-1 shows representative pressures, temperatures, and voltages maintained during solvent flow and helium flow operation of the ThermaBeam Mass Detector. Extractor Prequad Lens (–36 V) Filters (–19 V) Exit Lens Electron Multiplier (–800 to –2500 V) –100 V... -
Page 62: Opening And Shutting Valves With Cold Trap
V2 SHUT Filaments The ThermaBeam Mass Detector has two filaments; one filament is used as a backup. If the operational filament burns out, the other filament can be activated manually from the MS software without opening the vacuum chamber (see Section 5.2.1, Switching to the... -
Page 63: Lc Flow Rate Considerations
• Larger-than-standard-diameter (ID) fused silica capillary For a list of high-flow-rate configurations and components, see Table A-1. Attention: To prevent damage to the system, the ThermaBeam Mass Detector –4 STOP automatically shuts down if the vacuum chamber pressure rises above 5.0 x 10 torr. -
Page 64: Configuring Operating Conditions For Lc Flow Rates
If your flow rate is between 0.6 and 1.0 mL/min, a cold trap (no longer offered as a Waters option) is required as part of the vacuum system. Achieving optimum TMD performance at a desired LC flow rate depends on a variety of parameters that must be compatible with each other. -
Page 65: Setting The Helium Flow Rate
Table 2-4 indicates the values of the parameters that you must use to achieve optimal TMD performance at specific LC flow rates. Table 2-4 Operating Conditions and Flow Rates Fused Expansion Column Helium Nebulizer LC Flow Rate Silica Region Flow Rate Temperature (mL/min) Capillary... -
Page 66: Helium Flowmeter
The pressure gauge on the helium supply tank regulator must be set between 80 and 85 psi (552 and 586 kPa). Control Systems in the ThermaBeam Mass Detector... -
Page 67: Hardware Control
• Helium valve • Pirani gauge • Cold cathode vacuum gauge • Vacuum interlock gauge 2.5.1 Internal Electronics The ThermaBeam Mass Detector contains the following major electronic subassemblies: • Power supplies (dc and ac) • Controller printed circuit board Hardware Control... -
Page 68: Dc Power Supply
The analog printed circuit board provides supply voltages to the following TMD circuitry: • Filament • Ion optics • High voltage dc raw (unfiltered) • Detector high voltage • Radio frequency and dc resolution (for the quadrupole controller) Control Systems in the ThermaBeam Mass Detector... -
Page 69: Diverter Valve Assembly
Turbomolecular Pump Controller The turbomolecular pump controller contains circuitry to control the turbomolecular pump, which maintains the vacuum in the vacuum chamber. Quadrupole Controller The quadrupole controller generates the high voltage rf potentials required to operate the quadrupole mass filter. Nebulizer Power Supply The nebulizer power supply powers the nebulizer heater and the expansion region heater and thermocouples. -
Page 70: Fail-Safe Operation
The helium solenoid within the diverter valve turns helium flow on and off to the nebulizer assembly. Fail-Safe Operation To protect the ThermaBeam Mass Detector, the diverter valve is set automatically To Waste when helium is being supplied to the valve and any of the following conditions also occur: •... -
Page 71: Helium Valve
When any of these conditions occurs and the diverter valve is in the To Interface position, the MS OK contacts on the rear panel of the ThermaBeam Mass Detector close, signalling the LC flow (from the Waters 2690/2695 Separations Module) to stop. -
Page 72: Cold Cathode Vacuum Gauge
You need to clean or rebuild the cold cathode vacuum gauge periodically as described in Section 6.7.5, Rebuilding the Cold Cathode Vacuum Gauge. Vacuum Interlock Cold Cathode Top Rear Gauge Vacuum Gauge Inside View TP01258 Figure 2-5 Cold Cathode Vacuum Gauge and Vacuum Interlock Gauge Control Systems in the ThermaBeam Mass Detector... -
Page 73: Vacuum Interlock Gauge
The MS software performs the following functions: • Defines control and data acquisition parameters for the ThermaBeam Mass Detector. • Allows you to tune and optimize the ThermaBeam Mass Detector for optimum performance. • Allows you to create an instrument method, a processing method, and a report method. -
Page 74: Tuning The Thermabeam Mass Detector
2.6.1 Tuning the ThermaBeam Mass Detector Before you run samples through the ThermaBeam Mass Detector, you need to tune the detector. Tuning the ThermaBeam Mass Detector involves setting: • The lens element voltages for maximum sensitivity • The mass resolution •... -
Page 75: Optimizing The Thermabeam Interface
System, based on a thorough understanding of mass spectrometry. For a detailed description of how to use Manual Tune, see the tutorial provided in the Waters Integrity System Getting Started Guide, and the “Running Manual Tune” topic in the Empower Help. -
Page 76: Reporting Ms Data
Figure 2-6 Run Samples For detailed information about using Run Samples, see the “Acquiring Data in Run Samples” topic in the Empower Help, and in the Waters Integrity System Getting Started Guide. 2.6.4 Reporting MS Data Once you have acquired data and processed your results, you can create reports. You can customize how your reports are generated and how they look to conform to internal or regulatory standards. -
Page 77: Installing The Thermabeam Mass Detector
The Integrity System is shipped in several containers on pallets. This section describes how to unpack and inspect the ThermaBeam Mass Detector. For a description about how to unpack and inspect other components in the Integrity System, see the manuals that come with each component. -
Page 78: Installation Site Requirements
Save the shipping cartons should you need to transport or ship any of these components in the future. Inspecting Inspect the ThermaBeam Mass Detector and all related parts for physical damage. For information about reporting shipping damage, see the guide Waters Licenses, Warranties, and Support. -
Page 79: Integrity System Installation Site Requirements
Requirement Site (benchtop) Surface capable of supporting ThermaBeam Mass Detector (150 pounds [68 kg]) plus the combined weight of the Waters 2690/2695 Separations Module, 996/2996 Photodiode Array Detector, and the computer (300 pounds [136 kg]). Smooth, flat surface of approximately the following dimensions: •... -
Page 80: Required Materials For Installation
To prevent overheating of the vacuum pumps, ensure that the ambient STOP temperature does not exceed 40 °C (104 °F). 3.3 Required Materials for Installation You need the following materials to install the ThermaBeam Mass Detector and the vacuum system: • Startup Kits for: – ThermaBeam Mass Detector... -
Page 81: Installing The Vacuum System
– Vacuum Pump – 2690/2695 Separations Module – 2690/2695 Column Heater – 996/2996 Photodiode Array Detector – Column Bypass Module – Computer • Tools: – 5/16-inch open-end wrench – Small knife – Wire cutters ® – ChannelLock Pliers – Allen wrenches (3-mm and 4-mm) ®... - Page 82 Figure 3-1 shows how the vacuum pumps and the (optional) cold trap appear when they are installed and connected to the front of the ThermaBeam Mass Detector. The TMD can be installed and configured using the rear vacuum hose connections also.
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Page 83: Vacuum Pump Configuration
Cold Trap (Optional; No Longer Available) TP01440 Figure 3-1 Vacuum Pump Configuration Note: The procedure for connecting the signal cable from the ThermaBeam Mass Detector to the Pirani gauge is described in Section 3.8.2, Connecting the Pirani Gauge Signal Cable. -
Page 84: Setting Up The Vacuum Pumps
• Acceptable level of noise in the working environment. (For example, inside a well ventilated cabinet directly below the ThermaBeam Mass Detector, or through a partition into another room.) • Sufficiently isolated from the ThermaBeam Mass Detector to protect the detector from the heat and the vibration produced by the pumps. Safety Precautions Note the following with regard to the rotary-vane vacuum pumps: •... -
Page 85: Adding Oil To The Vacuum Pumps
• Hose clamps Swing Clamp Vacuum Hose O-Ring Hose Clamp NW25 Butt Flange NW25 Center Ring TP01342 Figure 3-2 Vacuum System Fittings Tight seals are critical to proper vacuum system performance. When you make connections in the vacuum system, ensure that there are no leaks and that the O-rings are not over-compressed, nicked, or scratched. -
Page 86: Required Materials
• Vacuum pump oil (shipped with the vacuum pumps) • Funnel • Rubber gloves • Pliers Attention: Use only oil recommended by Waters for the vacuum pumps; refer to STOP Appendix B for part numbers. When handling oil, wear rubber gloves to avoid skin contact with the oil. -
Page 87: Checking The Vacuum Pump Voltage Setting
MAX Oil Level Sight Glass MIN Oil Level TP01199 Figure 3-4 Filling the Oil Note: The oil level may drop about 10% after you power on the pump. This is a normal occurrence. 3. Install the oil filler plug and finger-tighten. 4. -
Page 88: Changing The Voltage Setting
(Figure 3-5). 4. Remove the cover plate (Figure 3-5). Cover Plate Power Switch TP01309 Figure 3-5 Removing the Cover Plate of the Vacuum Pump 5. Lift out the voltage indicator molding (Figure 3-6). Installing the ThermaBeam Mass Detector... -
Page 89: Checking The Rotation Of The Vacuum Pump Motor
Voltage Selector Switch Voltage Indicator Top View Molding of Vacuum Pump with Cover Plate Removed TP01319 Figure 3-6 Voltage Indicator Molding 6. Press the voltage selector switch to the opposite position (see Figure 3-6). 7. Reinstall the voltage indicator molding (it will fit only one way depending on how the switch is set). -
Page 90: Installing The Isolation Valves And The Pirani Gauge
Figure 3-7 Checking the Rotation of the Vacuum Pump Motor 3. If the fan does not rotate in the correct direction, power off the vacuum pump immediately with the power switch and contact your Waters Technical Service Representative. For more information about how to check the rotation of the pump motor, see the pump manufacturer’s manual. - Page 91 Required Materials You need the following materials from the Vacuum Pump Startup Kit to install the isolation valves and the Pirani gauge: • Isolation valve • Pirani gauge • NW25 elbow (2 each) • NW25 tee (2 each) • NW25 center rings with Viton O-rings (4 each) •...
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Page 92: Installing An Isolation Valve And The Pirani Gauge On The Stage 2 Vacuum Pump
To connect vacuum hoses to the outlet on the isolation valve NW25 tee, see Section 3.4.10, Connecting the Vacuum Hoses. Figure 3-9 shows the isolation valve and the Pirani gauge after installation on the Stage 2 vacuum pump. Installing the ThermaBeam Mass Detector... -
Page 93: Pirani Gauge And Isolation Valve On The Stage 2 Vacuum Pump
NW25 Tee Isolation Valve Pirani Gauge NW25 Elbow NW25 Tee TP01434 Figure 3-9 Pirani Gauge and Isolation Valve on the Stage 2 Vacuum Pump Stage 1 Pump Connections To install an isolation valve on the Stage 1 vacuum pump: 1. Use an inlet filter (with O-ring) and a swing clamp to attach an NW25 tee to the inlet port on the Stage 1 vacuum pump (Figure 3-10). -
Page 94: Installing The Isolation Valve On The Stage 1 Vacuum Pump
Ensure that the total length of the hose that connects the Stage STOP 1 pump to the ThermaBeam Mass Detector does not exceed 6 feet (1.83 m). Ensure that the hoses from the inlet and the outlet on the cold trap (if installed) to the Stage 1 pump do not each exceed 3 feet (0.91 m). -
Page 95: Connecting The Oil Mist Filters
To connect a vacuum hose from the outlet on the cold trap (if installed) to the NW25 tee connected below the isolation valve, see Section 3.4.11, Installing the Cold Trap (No Longer Available). 3.4.6 Connecting the Oil Mist Filters The vacuum pumps feature automatic oil recovery systems. A length of tubing that connects the oil mist filter to the gas ballast/oil return system returns the condensed oil to the pump reservoir. -
Page 96: Connecting The Gas Ballast/Oil Return
Required Materials You need the following materials from the Pump Startup Kit to install the gas ballast/oil return assembly: • Gas ballast/oil return • O-ring assemblies (as required) • Drain adapter and bonded seal Installing the ThermaBeam Mass Detector... -
Page 97: Gas Ballast Control Knob
• Oil return tubing • Inline restrictor • Hose clamp Procedure To install the gas ballast/oil return assembly: 1. Turn the gas ballast control knob on the Stage 1 vacuum pump to position (Figure 3-12). 2. Push down on the knob (against the spring) while turning the knob counterclockwise. -
Page 98: Installing The Gas Ballast/Oil Return On A Vacuum Pump
Secure the tubing with a hose clamp. 11. Repeat steps 1 through 10 for the Stage 2 pump. Figure 3-14 shows a vacuum pump with the gas ballast/oil return and oil mist filter assembly installed. Installing the ThermaBeam Mass Detector... -
Page 99: Connecting The Exhaust Hoses
To maintain proper vacuum, ensure that the length of vacuum hose between each pump and the ThermaBeam Mass Detector does not exceed 6 feet (1.83 m). Also ensure that the length of exhaust hose for a vacuum pump does not exceed 20 feet (6.1 m). Avoid sharp bends and low areas in the vacuum and exhaust hoses that could collect fluid. -
Page 100: Installing Exhaust Hoses
5. Repeat steps 1 to 4 for the Stage 2 pump. 6. Use nylon tie wraps and tie wrap bases to secure the exhaust hoses to the underside of the bench (or other suitable area) so that the exhaust hoses do not drop downward. Installing the ThermaBeam Mass Detector... -
Page 101: Connecting The Exhaust Trap Bucket
To Y-Connector into Exhaust Trap Inlet Port Exhaust Hose Exhaust Hose Stage 1 Vacuum Pump Stage 2 Vacuum Pump TP01434 Figure 3-16 Vacuum Pump Exhaust Hose Connections 3.4.9 Connecting the Exhaust Trap Bucket The inlet port of the exhaust trap bucket is connected to the oil mist filter of each vacuum pump to collect condensed solvent vapors and oil. -
Page 102: Connecting The Vacuum Hoses
The ThermaBeam Mass Detector is connected to each external vacuum pump through a vacuum hose. These vacuum hoses connect to the ThermaBeam Mass Detector at outlet ports on either the front or the rear of the ThermaBeam Mass Detector, as shown in Figure 3-18. -
Page 103: Vacuum Pump Outlet Ports
Front View Stage 1 Pirani Gauge Stage 2 Signal Cable Connector Turbo Back Rear View Helium Line Turbo Back Pirani Gauge Stage 2 Signal Cable Connector Stage 1 Figure 3-18 Vacuum Pump Outlet Ports Required Materials You need the following materials from the Vacuum Pump Startup Kit to connect the vacuum hoses: •... - Page 104 3. Insert a stainless steel tube with an attached O-ring into each outlet port. Make sure that each tube is seated firmly in the outlet port. 4. Place the compression plate over the three stainless steel tubes and screw the plate evenly into place. Installing the ThermaBeam Mass Detector...
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Page 105: Vacuum Outlet Port Connections (Front)
5. To connect a vacuum hose between the Stage 1 vacuum pump and the ThermaBeam Mass Detector: a. Cut a length of vacuum tubing long enough to connect between the vacuum port on the ThermaBeam Mass Detector and the corresponding vacuum pump inlet port. Attention:... -
Page 106: Installing The Cold Trap (No Longer Available)
Repeat steps 5a through 5e to connect a vacuum hose to each of the Stage 2 outlets of the ThermaBeam Mass Detector. b. Use an NW25 center ring and a swing clamp to connect the butt flange end of the... - Page 107 Attention: Before you install a cold trap, verify that the cold trap is configured for the STOP required voltage. For more information, see the cold trap manufacturer’s manual. Note: To ensure that the cold trap reaches operational temperature, provide sufficient distance (approximately 18 inches [45.7 cm]) between the cold trap and the vacuum pumps.
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Page 108: Connecting Isolation Valves To The Cold Trap
Center Ring Outlet Port Inlet Port Acrylic Flange Baffle Tube Flange Seal Figure 3-21 Connecting Isolation Valves to the Cold Trap Figure 3-22 shows the isolation valves installed on the acrylic flange of the cold trap. Installing the ThermaBeam Mass Detector... -
Page 109: Isolation Valves On The Cold Trap
Vacuum Hoses Isolation Isolation Valve Valve Outlet (V2) Inlet (V3) Acrylic Flange Baffle Tube TP01324 Figure 3-22 Isolation Valves on the Cold Trap Labeling the Isolation Valves 1. Use the marker set number 2 to label the cold trap outlet isolation valve (see Figure 3-22). -
Page 110: Cold Trap (No Longer Available)
2. Use an NW25 center ring, NW25 butt flange, and a hose clamp to connect the other end of the vacuum hose to the isolation valve on outlet port of the cold trap (Figure 3-24). Installing the ThermaBeam Mass Detector... -
Page 111: Connecting The Cold Trap To The Stage 1 Pump
To Exhaust Trap Bucket Inlet Port Outlet Port Upper NW25 Tee Lower NW25 Tee Stage 1 Vacuum Cold Trap Pump TP01431 Figure 3-24 Connecting the Cold Trap to the Stage 1 Pump 3. As shown in Figure 3-21, use an NW25 center ring, NW25 butt flange, and a hose clamp to connect a vacuum hose to the NW25 tee below the isolation valve on the Stage 1 pump (Figure... -
Page 112: Handling Mobile Phases
Table 2-1. 3.6 Making Fluidic Connections This section describes how to connect the LC fluid lines to the ThermaBeam Mass Detector. The topics covered include: • Installing tubing and compression fittings Installing the ThermaBeam Mass Detector... -
Page 113: Installing Tubing
• Connecting LC fluid lines • Installing the fused silica capillary • Installing other LC fluid lines 3.6.1 Installing Tubing Guidelines When you install tubing and compression fittings, use good laboratory practices and note the following: • Handle tubing carefully. Do not nick or sharply bend the tubing. This restricts flow and repeated bending causes tubing failure. -
Page 114: Fluidic Connections Overview
Make sure that you connect the tubing carefully to ensure a low-dispersion connection. Figure 3-26 is a schematic of all the LC fluidic connections in the Integrity System. For a list of the tubing shown in Figure 3-26, see Section B.2, Integrity System Tubing. Installing the ThermaBeam Mass Detector... -
Page 115: Installing The Fused Silica Capillary
Waste Module Low Volume Flow Cell Vent Transducer In-line Sample Filter Loop ThermaBeam Mass Detector Injector In-line 25 µl N e b u l i z e r Filter Syringe A s s e m b l y Seal Union... - Page 116 • Open-end wrenches, 5/16-inch and 9/16-inch • Union between nebulizer and diverter valve • Stainless steel tubing • Cotton swabs • Methanol Attention: To avoid contaminating the nebulizer, always wear gloves when installing the STOP fused silica capillary. Installing the ThermaBeam Mass Detector...
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Page 117: Section 3.10, Shutting Down
Procedure To install and cut the fused silica capillary: 1. Ensure that the system is shut down as described in Section 3.10, Shutting Down the Integrity System. 2. Remove the nebulizer as follows: a. Disconnect the nebulizer wiring harness. b. Disconnect LC tubing from the union on the nebulizer bracket (Figure 3-27). -
Page 118: Fused Silica Capillary In Nebulizer
(Figure 3-28). 13. Secure the compression screw in the bottom plate of the nebulizer, but do not tighten completely (Figure 3-28). Installing the ThermaBeam Mass Detector... -
Page 119: Installing The Fused Silica Capillary
14. Pull the fused silica capillary down just below the surface of the nebulizer tip. Wipe any excess polyamide coating material from the top surface of the nebulizer tip with a methanol-soaked cotton swab. Nebulizer Bracket Bottom Plate Compression Screws Ferrule PEEK Sleeve Slot... -
Page 120: Connecting The Capillary And Union
22. Lift the union and capillary out from the slot at the bottom of the bracket. Install one insulating washer below the union and place the union and washer into the slot in the side of the bracket (Figure 3-30). Installing the ThermaBeam Mass Detector... -
Page 121: Connecting The Union To The Nebulizer Bracket
23. On the outside of the bracket, install in sequence the second insulating washer, flat washer, spring washer, and hex nut to secure the union into the slot in the side of the bracket (Figure 3-30). Attention: To prevent the union from rotating while it is being secured, hold STOP the union in place with the 5/16-inch wrench while you tighten the nut with the 9/16-inch wrench. -
Page 122: Installing Other Lc Fluid Lines
To connect the LC fluid outlet line from the 996/2996 PDA Detector to the ThermaBeam Mass Detector diverter valve: 1. Pass the free end of the 996/2996 PDA Detector outlet fluid line through the opening in the left side panel of the ThermaBeam Mass Detector (Figure 3-32). -
Page 123: Connecting The Waste Line
Connecting the CBM to the 996/2996 PDA Detector To connect the Column Bypass Module (CBM) to the 996/2996 PDA Detector LC fluid inlet line, see the Waters Column Bypass Module Operator’s Guide, Section 1.3, Making Fluidic Connections. Making Fluidic Connections 123... -
Page 124: Connecting The Helium Supply
Tubing. 3.7 Connecting the Helium Supply The operation of the ThermaBeam Mass Detector requires a supply of helium to help nebulize LC effluent. To connect helium to the Column Bypass Module, see the Waters Column Bypass Module Installation and Maintenance Guide. -
Page 125: Helium Inlet Port
ThermaBeam Mass Detector, as shown in Figure 3-33. TMD Rear Panel TP01297 Figure 3-33 Helium Inlet Port 5. Open the front panel door of the ThermaBeam Mass Detector and locate the helium flowmeter and the nebulizer (Figure 3-34). Helium Supply to Nebulizer... -
Page 126: Helium Flowmeter Knob
Manual Tune window to be sure the helium valve in the TMD is on. 10. Turn the helium flowmeter knob counterclockwise until the flowmeter float indicates the required setting. Installing the ThermaBeam Mass Detector... -
Page 127: Making Electrical Connections
3.8.1 Making IEEE-488 Interface Connections Overview The ThermaBeam Mass Detector communicates with the Empower Personal workstation through the IEEE-488 bus interface. The other devices in the Integrity System connect to the IEEE-488 interface from the connector on the rear panel of the 2690/2695 Separations Module. -
Page 128: Cable Lengths
To prevent intermittent IEEE-488 communication failures, use the cables supplied with the system. The maximum allowable total cable length in one system is 6.5 feet (2 m) times the numbers of devices or 65 feet (20 m), whichever is smaller. Installing the ThermaBeam Mass Detector... -
Page 129: Ieee-448 Network Bus Addresses
Each device on the IEEE-488 network bus must have a unique address. Table 3-2 lists the addresses assigned to the Integrity System components at the factory. Table 3-2 IEEE-448 Network Bus Addresses Instrument Address Address-Setting Method ThermaBeam Mass Detector DIP switch 996/2996 PDA Detector DIP Switch 2690/2695 Separations Software Module Note: If you add or remove a device or power off a device, you must do a scan of all the connected busLAC/E instruments so they can be recognized by the software. -
Page 130: Ieee-488 Address Switch On The Thermabeam Mass Detector
Figure 3-37 IEEE-488 Address Switch on the ThermaBeam Mass Detector If the switches are not set correctly, set them to the correct positions, then reset the detector (power off, then power on the unit). Perform the IEEE-488 startup sequence as described in Section 3.9, Starting Up the... -
Page 131: Connecting The Pirani Gauge Signal Cable
Stage 2 vacuum pump (Figure 3-39). Connect one end of the cable to the front or rear 9-pin D-connector on the ThermaBeam Mass Detector. Connect the other end to the connector on the top of the Pirani gauge, as shown in Figure 3-39. -
Page 132: Connecting The Ms Ok Cable
3.8.3 Connecting the MS OK Cable The MS OK cable is a 2-wire event cable that transmits the MS OK output signal from the ThermaBeam Mass Detector to the Stop Flow input terminal on the 2690/2695 Separations Module. The MS OK output signal stops the sample flow if there is insufficient helium pressure to the mass spectrometer. - Page 133 4. Strip the white lead at the free end of the MS OK cable, insert it into the pin 5 slot on the ThermaBeam Mass Detector rear panel, and tighten the screw. 5. Strip the black lead, insert it into the pin 6 slot on the ThermaBeam Mass Detector rear panel, and tighten the screw.
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Page 134: Connecting The Ac Power Cord
To attach the ac power cord to the ThermaBeam Detector: 1. Verify that the ThermaBeam Mass Detector power switch (located on the lower- right front panel) is in the Off position. Installing the ThermaBeam Mass Detector... -
Page 135: Starting Up The Integrity System
Figure 3-41 AC Power Connection 3. Insert the power cord into a properly grounded ac power outlet. Attention: To prevent damage to the ThermaBeam Mass Detector, use only STOP the power cord supplied with the system (rated for 20 A). -
Page 136: Isolation Valve Settings For Tmd Startup (With Cold Trap)
For specific and necessary startup procedures, refer to the user’s manual or installation and maintenance guide for each of the instruments listed above. Immediately after you power on the ThermaBeam Mass Detector, it runs an internal diagnostic routine. If the ThermaBeam Mass Detector is functioning properly, the front panel indicator lights glow continuously. -
Page 137: Isolation Valve Settings For Tmd Startup (Without Cold Trap)
(shown in ) come on within 10 seconds after powering on the ThermaBeam Mass Detector. If the lights do not turn on, power off the TMD and shut all the isolation valves on the vacuum pumps. To diagnose the Chapter 5, Troubleshooting. -
Page 138: Shutting Down The Integrity System
5. Cold trap Shutting Down the TMD This section describes how to manually shut down the ThermaBeam Mass Detector. For more information about how to shut down the system and about how to create a method set to automatically perform shutdown, see the Waters Integrity System Getting Started Guide. -
Page 139: Run Samples
Figure 3-42 Run Samples 2. Select Diagnostics > TMD Manual Tune. The Manual Tune window appears (Figure 3-43). 3. In the TMD Control panel, select from the MS Enable list. This powers off the heaters, high voltage supplies, helium supply, and the filament. The LC fluid is diverted to waste. -
Page 140: Manual Tune Window With Ms Enable Off
To avoid burn injury, allow the heater components to cool down before you remove any parts. 5. Shut off power to the ThermaBeam Mass Detector with the main power switch on the lower-right front panel. The system vents to the atmosphere (through the vent valve) for approximately 2 minutes after power is shut off. -
Page 141: Isolation Valve Settings For Tmd Startup (With Cold Trap)
Table 3-5 Isolation Valve Settings for TMD Startup (with Cold Trap) Isolation Valve Valve Position V1 (Stage 1 Pump) Shut V2 (Cold Trap Inlet) Open V3 (Cold Trap Outlet) Open V4 (Stage 2 Pump) Open Table 3-6 Isolation Valve Settings for TMD Startup (without Cold Trap) Isolation Valve Valve Position V1 (Stage 1 Pump) -
Page 142: Running Diagnostic Tests
Chapter 4 Running Diagnostic Tests This chapter describes the diagnostic tools available for the ThermaBeam Mass Detector. The diagnostic tools include: • Startup tests • Front panel indicator lights • User-initiated diagnostic tests • Malfunction state • System Status window display... -
Page 143: Indicator Lights
4.2 Indicator Lights The front panel indicator lights (Figure 4-1) provide diagnostic information about the operation of the ThermaBeam Mass Detector. The ThermaBeam Mass Detector is operating properly when the Power, Interlock OK, and Status indicator lights illuminate continuously. TP01304... -
Page 144: Status Indicator Light
Interlock OK Indicator Light When the Interlock OK indicator light is on continuously, it indicates that the ac power switch is on and verifies the following ac interlock conditions: • The vacuum chamber lid is closed. • The internal safety panel is in place. •... -
Page 145: Troubleshooting With The Front Panel Indicator Lights
• Check outlet for power. • Check fuses. Fuse blown Section 6.7.2, Replacing Fuses. Fan failure Contact Waters Technical Service. Interlock OK indicator light The ac interlock switch Verify that the interior safety panel is in off, fan running. is open place and all covers are secured. -
Page 146: User-Initiated Diagnostic Tests
Section 4.2, Indicator Lights), contact Waters Technical Service at 800 252-4752, U.S. and Canadian customers only. Other customers, call your local Waters subsidiary or your local Waters Technical Service Representative, or call Waters corporate headquarters in Milford, Massachusetts (U.S.A.) for assistance. -
Page 147: Empower Quickstart: Run Samples Diagnostics Window
Figure 4-2 Empower QuickStart: Run Samples Diagnostics Window Figure 4-3 Empower Pro: Run Sample Diagnostics Window Running Diagnostic Tests... -
Page 148: Malfunction State
4.4 Malfunction State Overview ThermaBeam Mass Detector (TMD) firmware continuously monitors the vacuum chamber pressure and the compartment temperature of the TMD. The TMD enters the Malfunction state if the firmware detects either of the following conditions: –4 • Inadequate vacuum (greater than 5 × 10 torr) in the vacuum chamber •... -
Page 149: Manual Tune Error Message
In addition, the error message that is generated varies according to whether you are in the Manual Tune window or Run Samples when the Malfunction state occurs. In the Manual Tune Window If the TMD enters the Malfunction state while you are in the Manual Tune window, the following error message appears (Figure 4-5). -
Page 150: Inadequate Vacuum In Vacuum Chamber
In Run Samples If the TMD enters the Malfunction state while you are in Run Samples, Instrument failed appears in the System Status Message box at the bottom of the window, and Shutdown appears in the TMD status box at the right of the window (see Figure 4-6). -
Page 151: Tmd Diagnostics Window In The Malfunction State
Figure 4-7 TMD Diagnostics Window in the Malfunction State Using the Manual Tune Window When you select Diagnostics > TMD Manual Tune from Run Samples, the Manual Tune window will appear. It allows you to monitor the vacuum chamber pressure in the Temperatures and Pressures section of the window (Figure 4-8). -
Page 152: Tmd Compartment Temperature Failure
Figure 4-8 Manual Tune Window in the Malfunction State 4.4.2 TMD Compartment Temperature Failure When high TMD compartment temperature causes a Malfunction state, the following behavior may be seen: • The Message Center displays an Instrument Failure message. • When initially accessed, the TMD Diagnostics window first appears as shown in Figure 4-7, then displays a time-out error (Figure... -
Page 153: Invoking The Malfunction State For Troubleshooting
Figure 4-9 Time-Out Error Message Figure 4-10 Manual Tune Malfunction State Message 4.4.3 Invoking the Malfunction State for Troubleshooting You can intentionally invoke the Malfunction state to help you troubleshoot the Integrity System. To intentionally invoke the Malfunction state: 1. Power off the TMD, then power on the TMD. 2. -
Page 154: System Status Information
Note: In a client/server configuration, always shut down the network server first. 3. Power off the TMD. 4. Power on the TMD. 5. When the TMD status LED stops cycling and is steadily lit, power on the Empower Personal workstation. Note: In a client/server configuration, always start up the network server first. -
Page 155: System Status Information
Table 4-2 System Status Information MS Enable Category Subsystem MS Enable On MS Enable Off Standby System Status +28 Vdc QC Warmup In Progress/ In Progress/ In Progress/ Complete Complete Complete Enclosure Ok/Fail Ok/Fail Ok/Fail Temperature Pump Down Timer Ok/Wait Ok/Wait Ok/Wait Chamber Vacuum... -
Page 156: Displaying The System Status Dialog Box
4.5.2 Displaying the System Status Dialog Box The minimum configuration that allows the display of the System Status dialog box includes connection of the ThermaBeam Mass Detector to the following: • Stage 1 and Stage 2 vacuum pumps • Properly regulated helium supply •... -
Page 157: Run Samples
To display the System Status dialog box: 1. Access Run Samples by clicking Run Samples in the Empower Pro or QuickStart window, then selecting the appropriate chromatographic system. Run Samples appears (Figure 4-11). Figure 4-11 Run Samples Running Diagnostic Tests... -
Page 158: Manual Tune Window
2. Select Diagnostics > Manual Tune. The Manual Tune window appears (Figure 4-12). Figure 4-12 Manual Tune Window System Status Information 158... -
Page 159: Tmd System Status Dialog Box
3. Select View > System Status. The TMD System Status dialog box appears (Figure 4-13). Figure 4-13 TMD System Status Dialog Box Running Diagnostic Tests... -
Page 160: Chapter 5 Troubleshooting
Maintenance. Caution: To avoid the possibility of electric shock, do not remove the side or rear panels of the ThermaBeam Mass Detector. The ThermaBeam Mass Detector control circuitry and power supply do not contain user-serviceable components. Contacting Waters Technical Service If you encounter any problems when troubleshooting the TMD, contact Waters Technical Service at 800 252-4752, U.S. -
Page 161: Connecting Test Equipment
Section 4.5, System Status Information), you can gain other system operational information by connecting test equipment to the ThermaBeam Mass Detector. You connect test equipment at the Service Signal connector located on the rear panel of the ThermaBeam Mass Detector (Figure 5-1). -
Page 162: Service Signals
The Service Signal connector, shown in Figure 5-1, provides access to the following ThermaBeam Mass Detector service signals: • Sync – Synchronization for the ion signal oscilloscope trace. Connect to pin 4 (Sync terminal). • Ion Signal – Ion detector scan output signal for display on an oscilloscope. Connect to pin 3 (Ion Signal terminal). - Page 163 Table 5-1 Cold Cathode Vacuum Gauge Calibration Data (Continued) Output Voltage (V) Pressure (torr) –7 5.000 9.5 × 10 –6 5.200 1.2 × 10 –6 5.400 1.5 × 10 –6 5.600 1.9 × 10 –6 5.800 2.4 × 10 –6 6.000 2.9 ×...
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Page 164: Troubleshooting The Thermabeam Mass Detector
To avoid serious health and fire hazards, never spray (nebulized) solvent into the air. Preliminary Troubleshooting Steps To start troubleshooting the ThermaBeam Mass Detector: 1. Check the Empower Message Center for error messages. 2. Check the System Status dialog box for the current states of the instrument components (see Section 4.5.1, System Status... -
Page 165: Troubleshooting The Thermabeam Mass Detector
Detector but not with the TMD, then you know that the LC system is functioning properly but the TMD is not functioning properly. Table 5-2 lists some performance-related symptoms that may occur, their possible causes, and corrective actions. Table 5-2 Troubleshooting the ThermaBeam Mass Detector Symptom Possible Cause Corrective Action Status indicator light IEEE-488 not Check the IEEE-488 connections. - Page 166 Table 5-2 Troubleshooting the ThermaBeam Mass Detector (Continued) Symptom Possible Cause Corrective Action No peaks in Manual Improper tune or Load known good tune and look for peaks at 28 Tune, or Easy Tune optimization ) and 32 (O ), or with calibration gas on, at fails (signal level low 69 (PTA).
- Page 167 Table 5-2 Troubleshooting the ThermaBeam Mass Detector (Continued) Symptom Possible Cause Corrective Action Loss of chromato- Inadequate vacuum Check the vacuum system as described in graphic signal Section 5.3, Troubleshooting the Vacuum System. Dirty ion volume or Clean the ion volume as described in dirty ThermaBeam Section 6.6.2, Cleaning the Ionizer Compo-...
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Page 168: Switching To The Backup Filament
Table 5-2 Troubleshooting the ThermaBeam Mass Detector (Continued) Symptom Possible Cause Corrective Action Reduced response to Dynode not powered Power on the dynode in the Manual Tune high masses window (see the “Preparing the TMD for Tuning” topic in the Empower Help). -
Page 169: Switching Filaments In The Manual Tune Window
To switch to the backup filament: 1. In Run Samples, select Diagnostics > TMD Manual Tune. 2. Select from the MS Enable list (Figure 5-3). Figure 5-3 Switching Filaments in the Manual Tune Window 3. Click the Filament 2 option button to activate Filament 2. If you were using Filament 2, then activate Filament 1. -
Page 170: Verifying Helium Flow
Observe the peak pattern in the Manual Tune window for a representative PTA ion mass spectrum. 5.2.2 Verifying Helium Flow This section describes how to verify helium flow in the ThermaBeam Mass Detector. To verify adequate nebulizer gas flow: 1. In the Manual Tune window, select... -
Page 171: Verifying Helium Flow In The Manual Tune Window
Figure 5-4 Verifying Helium Flow in the Manual Tune Window 3. Select He Valve to turn on the nebulizer gas flow. 4. Check that the ball float in the helium flowmeter is at the correct setting (see Section 2.4, Setting the Helium Flow Rate). -
Page 172: Troubleshooting The Vacuum System
5.3 Troubleshooting the Vacuum System Problems with the vacuum system can occur because of: • Air leaks – Interfere with timely evacuation of the chamber and may prevent attaining sufficient vacuum. • Water adsorption – A vacuum system that has been open to the atmosphere for several hours can adsorb a significant amount of water vapor. -
Page 173: Troubleshooting The Vacuum System
Table 2-1, Opening and Shutting Isolation and the cold trap not Valves. in the correct position Stage 1 or Stage 2 Contact Waters Technical Service. vacuum pump failure Leak in the Therma- Check the condition of the O-rings in the inter- Beam Mass Detector face assembly. - Page 174 Table 5-3 Troubleshooting the Vacuum System (Continued) Symptom Possible Cause Corrective Action Interface pressure too Isolation valve on Switch the isolation valve to the Open position. low (less than 1.5 × Stage 2 pump closed –2 torr, with no LC or Fixed leak filter Change the fixed leak filter as described in helium flow)
- Page 175 Section 6.7.5, Rebuilding the Cold Cathode Vacuum Gauge. Turbomolecular pump Contact Waters Technical Service. problem Chamber pressure too Excessive helium flow Ensure the helium inlet pressure on the helium high when helium tank regulator is set between 80 and 85 psi valve open (552 and 586 kPa).
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Page 176: Locating And Repairing Air Leaks
Vacuum oil discolored Change the oil (see Section 6.2.2, Changing the Vacuum Pump Oil). Vacuum pump failure Contact Waters Technical Service. Vent valve not Contact Waters Technical Service. functioning 5.3.1 Locating and Repairing Air Leaks Air leaks can: • Result in inadequate chamber vacuum •... - Page 177 To prevent air leaks at these sites, wipe the O-ring with 100% methanol and dry with a lint-free cloth before you: • Close the vacuum chamber lid • Assemble the nebulizer Determining the Size of an Air Leak To determine the size of an air leak: 1.
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Page 178: Detecting A Failure In The Vacuum Pumps
Attention: To avoid damage to the system, do not use vacuum grease on any STOP connections in the ThermaBeam Mass Detector. Repairing Compression Fittings To correct a leak at a compression fitting: 1. Further tighten the fitting using only a modest amount of torque. If this does not fix the leak, do not continue tightening. -
Page 179: Troubleshooting The Ieee-488 Interface
Section 6.2.2, Changing the Vacuum Pump Oil. If none of these actions corrects the problem, contact Waters Technical Service at 800 252-4752, U.S. and Canadian customers only. Other customers, call your local Waters subsidiary or Technical Service Representative, or call Waters corporate headquarters in Milford, Massachusetts (U.S.A.) for assistance. - Page 180 Table 5-5 Troubleshooting IEEE-488 Bus Communications (Continued) Symptom Possible Cause Corrective Action ThermaBeam Mass Detector not Incorrect bus Ensure that the IEEE-488 address is set responding to the Empower address correctly by the DIP switches on the rear Personal workstation (continued) panel of the TMD.
- Page 181 Enter Run Samples, then check the inside TMD Message Center for more information as cabinet too high described in Section 4.4, Malfunction State. If the cabinet temperature is too high, shut down the Integrity System and contact Waters Technical Service. Troubleshooting...
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Page 182: Chapter 6 Maintenance
Contacting Waters Technical Service If you encounter any problems replacing parts in the TMD, contact Waters Technical Service at 800 252-4752, U.S. and Canadian customers only. Other customers, call your local Waters subsidiary or Technical Service Representative, or call Waters corporate headquarters in Milford, Massachusetts (U.S.A.) for assistance. -
Page 183: Shutting Down The Thermabeam Mass Detector
Cold trap (optional) outlet line (V2) c. Stage 1 vacuum pump (V1) d. Stage 2 vacuum pump (V4) 3. Power off the ThermaBeam Mass Detector by turning the power switch on the lower right front panel to the position. -
Page 184: Starting Up The Thermabeam Mass Detector
Open b. Stage 2 vacuum pump (V2) – Open 4. Power on the ThermaBeam Mass Detector by turning the ac power switch on the lower right front panel to the position. 5. Reboot the computer so that the Empower software recognizes the ThermaBeam Mass Detector. -
Page 185: Maintaining The Vacuum Pumps
Note: The TMD may take up to 1 hour to reach the proper vacuum level. System diagnostics report a failure if the system fails to reach the proper vacuum level. You can read the vacuum pressure in the Empower MS Manual Tune window or in Run Samples. Note: Do not access the Manual Tune or Run Samples windows until the TMD Status light is continuously lit, or until the TMD is in the Malfunction state. -
Page 186: Vacuum System Fittings
• Material Safety Data Sheets for vacuum oil and for the chemicals that you are using in your application Vacuum System Fittings You install components of the vacuum system, such as vacuum hoses, exhaust hoses, and isolation valves, using the following fittings: •... -
Page 187: Checking The Vacuum Pump Oil
Change the oil every 2 to 4 weeks, or more frequently if the oil turns dark in color. Waters recommends that you change vacuum pump oil when you do other maintenance. Power off only the pump on which you are doing maintenance. Change the oil while the oil is still warm. -
Page 188: Required Materials
Required Materials You need the following materials to change the oil: • Oil (use only the oil recommended by Waters) • Rubber gloves • Container in which to drain the oil (at least 1-L capacity) • Funnel (if required for container type) - Page 189 3. Elevate the pump at least 6 to 8 inches (15.2 to 20.3 cm) off the supporting surface if it is not already elevated. Caution: To avoid lifting or burn injury, use caution when lifting the vacuum pump. Lift the pump by the lifting bracket using suitable lifting equipment. Be sure to wear gloves.
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Page 190: Emptying The Exhaust Trap Bucket
Oil Filler Plug MAX Oil Level Sight Glass MIN Oil Level TP01199 Figure 6-4 Filling the Oil 12. Allow a few minutes for the oil to drain into the pump and recheck the level. Add more oil as necessary, but avoid overfilling the pump. Note: Be aware that it is normal for the oil level to drop about 10% after you power on the pump. -
Page 191: Emptying The Exhaust Trap Bucket
To clean the Pirani gauge: 1. Ensure that the system is shut down as described in Section 6.1.1, Shutting Down the ThermaBeam Mass Detector. 2. Ensure that the Stage 2 vacuum pump isolation valve (V4) is in the Shut position. -
Page 192: Removing The Pirani Gauge
4. Remove the swing clamp and NW25 center ring from the base of the Pirani gauge to remove the gauge from the NW25 elbow (Figure 6-6). Pirani Gauge Swing Clamp TP01335 Figure 6-6 Removing the Pirani Gauge 5. Remove the C-ring and screen from the bottom of the gauge with the snap-ring pliers (Figure 6-7). -
Page 193: Changing The Oil Mist Filter Elements
6. Hold the gauge upside down and pour 5 to 10 mL of methanol into the opening of the gauge. 7. Swirl the methanol inside the gauge for 1 to 2 minutes. 8. Pour out the methanol from the inside of the gauge. 9. -
Page 194: Removing The Oil Mist Filter
6-8): 1. Shut down the system as described in Section 6.1.1, Shutting Down the ThermaBeam Mass Detector. 2. Power off the Stage 1 vacuum pump with the power switch on the side of the pump, then unplug the power cord to the vacuum pump from the ac outlet. -
Page 195: Accessing The Oil Mist Filter Element
Oil Mist Filter Element Figure 6-9 Accessing the Oil Mist Filter Element 5. Remove the filter element and dispose of it properly. 6. Wipe clean the inside of the oil mist filter housing and the outside if necessary. 7. Check that the O-ring on the bottom of the new filter element is seated correctly, then insert the filter element (Figure 6-10). -
Page 196: Inserting A New Oil Mist Filter Element
O-Ring Figure 6-10 Inserting a New Oil Mist Filter Element 8. Rejoin the two halves of the oil mist filter housing. 9. Reinstall and secure the four Allen screws. 10. Plug the power cord to the vacuum pump in the ac outlet. 11. -
Page 197: Changing The Oil Wick Cartridge
Section 6.1.1, Shutting Down the ThermaBeam Mass Detector. 2. Open the front door of the ThermaBeam Mass Detector to access the safety panel. Remove the screws that hold the safety panel in place and remove the safety panel (see Figure 6-11). -
Page 198: Moving The Diverter Valve Assembly
3. Remove the nebulizer (as described in Section 6.5, Cleaning LC/MS Interface Components). 4. Disconnect the divert valve assembly by unscrewing the screws on the bottom right of the assembly and on the rear panel of the assembly (Figure 6-12). 5. -
Page 199: Accessing The Turbomolecular Pump
To avoid oil contamination, be careful not to dip oil on the inside STOP of the ThermaBeam Mass Detector. 10. Insert the new oil wick cartridge in the bottom of the turbomolecular pump. 11. Reinstall the bottom plate of the turbomolecular pump by using the turbo pump spanner tool to turn the plate clockwise. -
Page 200: Defrosting The Optional Cold Trap
15. Reinstall the nebulizer, as described in Section 6.5, Cleaning LC/MS Interface Components. 16. Reinstall the safety panel and close the front door of the ThermaBeam Mass Detector. 17. Start up the system as described in Section 6.1.2, Starting Up the ThermaBeam Mass Detector. -
Page 201: Drain Outlet On The Cold Trap
3. Power off the cold trap with the power switch on the front of the unit (Figure 6-14). 4. Elevate the cold trap to make it easier to drain the liquid. 5. Place a container under the drain outlet (Figure 6-14). -
Page 202: Removing The Flange Seal From The Cold Trap
Isolation Valve Isolation Valve Acrylic Flange Baffle Assembly TP01324 Figure 6-15 Removing the Flange Seal from the Cold Trap 8. Close the drain valve. 9. Pour warm water at a maximum temperature of 50 °C (122 °F) into the condenser chamber. -
Page 203: Cleaning Lc/Ms Interface Components
To remove the nebulizer, the expansion region, and the momentum separator (Figure 6-17): 1. Shut down the system as described in Section 6.1.1, Shutting Down the ThermaBeam Mass Detector. Caution: To avoid burn injury, allow the nebulizer to cool before you disassemble it. -
Page 204: Disconnecting The Nebulizer
Wiring Harness Connector Helium Tubing from Flowmeter Tubing to Diverter Valve TP05020 Figure 6-16 Disconnecting the Nebulizer 5. Turn the nebulizer counterclockwise to disengage the locking pins, and then pull the nebulizer down and away from the expansion region (Figure 6-17). -
Page 205: Cleaning The Nebulizer
Installing the Fused Silica Capillary. 8. Remove the expansion region by turning the collar on the expansion region counterclockwise (Figure 6-18). 9. Pull the expansion region and momentum separator down and away from the ThermaBeam Mass Detector (Figure 6-18). Collar Expansion Region TP01328... -
Page 206: Removing The Momentum Separator From The Expansion Region
Attention: To avoid contaminating the LC/MS interface, always wear clean STOP gloves when you handle the momentum separator. Powder-free rubber gloves can be used if you are sure that components have cooled sufficiently; otherwise use lint-free cloth gloves. 10. Unscrew the momentum separator (counterclockwise) to remove it from the expansion region (Figure 6-19). -
Page 207: Cleaning The Expansion Region
3. Use a cotton swab with pumice and distilled water to clean the top surface of the expansion region (Figure 6-20). Use a small, 1/16-inch nylon brush to clean the aperture of the expansion region. Aperture Inner Surfaces TP01300 Figure 6-20 Cleaning the Expansion Region 4. -
Page 208: Cleaning The Momentum Separator
TP01299 Figure 6-21 Cleaning the Momentum Separator 4. Rinse with hot distilled water. 5. Rinse thoroughly with methanol to displace the water. 6. Sonicate the momentum separator in a beaker of distilled water for 5 minutes and then in a beaker of methanol for 5 minutes. 7. -
Page 209: Cleaning Vacuum Chamber Components
6.6.1 Replacing a Filament The ThermaBeam Mass Detector has two filaments; one filament is used as a backup. If a filament fails during a run, you can manually switch to the other filament without opening the vacuum chamber (the run is not interrupted). -
Page 210: Replacing The Filament
Note: To reduce the amount of water adsorbed by the system, keep the vacuum chamber lid closed and latched whenever possible when you are performing maintenance on chamber components. Caution: To avoid burn injury, allow ion source temperatures to cool before you handle the filament. -
Page 211: Cleaning The Ionizer Components
Removing and Cleaning the Ion Volume This procedure describes how to clean the ion volume subassembly. To remove and clean the ion volume: 1. Shut down the system as described in Section 6.1.1, Shutting Down the ThermaBeam Mass Detector. Maintenance... -
Page 212: Removing The Ion Volume
2. Open the vacuum chamber lid after the vacuum chamber has vented. Note: To reduce the amount of water adsorbed by the system, keep the vacuum chamber lid closed and latched whenever possible when you are performing maintenance on chamber components. Caution: To avoid burn injury, allow ion source temperatures to cool before you disassemble the ion source. -
Page 213: Cleaning The Ion Volume
11. Proceed to the next two discussions if you need to clean the remaining parts of the ionizer and the extractor lens. Otherwise, close the vacuum chamber lid and start up the system as described in Section 6.1.2, Starting Up the ThermaBeam Mass Detector. Removing and Cleaning the Ionizer To remove and clean the ionizer: 1. -
Page 214: Disconnecting The Ionizer Wiring Harness
Note: To reduce the amount of water vapor adsorbed by the system, keep the vacuum chamber lid closed and latched whenever possible when you are performing maintenance on chamber components. Caution: To avoid burn injury, allow ion source temperatures to cool before you disassemble the ion source. -
Page 215: Removing The Ionizer
W at er s TP01322 Figure 6-26 Removing the Ionizer 5. Remove the filaments from the Vespel™ holder by pulling them straight up and away from the ionizer (see Figure 6-23). 6. Remove the ion volume from the ionizer by pressing on the retaining spring and pulling the ion volume straight out from the ionizer (see Figure 6-23). -
Page 216: Cleaning The Ionizer
Ionizer Wiring Filament Harness Channels Aperture TP01287 Figure 6-27 Cleaning the Ionizer 11. Use a cotton swab with pumice and distilled water to clean the filament channels (see Figure 6-27). 12. Use a cotton swab with pumice and distilled water to clean the aperture at the base of the ionizer (see Figure 6-27). -
Page 217: Cleaning The Extractor Lens
TP01289 Figure 6-28 Cleaning the Extractor Lens 2. Rinse the extractor lens thoroughly with distilled water to remove all traces of pumice. 3. Rinse the extractor lens thoroughly with methanol to displace the water. 4. Dry thoroughly with a lint-free cloth. Reinstalling the Ionizer To reinstall the ionizer: 1. -
Page 218: Cleaning The Quadrupole Mass Filter
Procedure To clean the quadrupole mass filter: 1. Shut down the system as described in Section 6.1.1, Shutting Down the ThermaBeam Mass Detector. 2. Open the vacuum chamber lid after the vacuum chamber has vented. Note: To reduce the amount of water vapor adsorbed by the system, keep the vacuum chamber lid closed and latched whenever possible when you are performing maintenance on chamber components. -
Page 219: Disconnecting The Quadrupole Mass Filter
Wiring Thumbscrews TP01259 Harness and Clamps Figure 6-29 Disconnecting the Quadrupole Mass Filter 5. Disconnect the wiring harness from the vacuum chamber wiring harness connector (Figure 6-29). 6. Remove the quadrupole canister from the vacuum chamber (Figure 6-30). TP01323 Figure 6-30 Removing the Quadrupole Mass Filter Attention: To prevent damage to the quadrupole mass filter, take STOP... -
Page 220: Cleaning The Quadrupole Mass Filter
7. Use the nylon bristle brush, pumice, and distilled water to clean between the prequad filters and the quadrupole poles (Figure 6-31). Rotate the canister as you run the brush up and down the center of the quadrupole poles. Note: Use only the nylon bristle brush supplied in the Integrity System Startup Kit. -
Page 221: Miscellaneous Maintenance Procedures
6.7.2 Replacing Fuses This section describes how to change fuses on the ThermaBeam Mass Detector. The ThermaBeam Mass Detector fuses are located in the fuse holder on the rear panel just below the ac power cord connector. To replace the fuses on the ThermaBeam Detector: 1. -
Page 222: Replacing The Fixed Leak Filter
Replace fuses only with fuses of the same type and rating of the STOP previous fuses. To check the correct fuse rating, see the rear panel of the ThermaBeam Mass Detector 5. Reinstall the fuse holder. 6. Reinstall the power cord in the ac outlet. -
Page 223: Refilling The Calibrant Vial
1. Shut down the system as described in Section 6.1.1, Shutting Down the ThermaBeam Mass Detector. 2. Open the front door of the ThermaBeam Mass Detector and remove the safety panel (see Figure 6-11). 3. Use the TORX screwdriver to remove the filter from the end of the fixed leak tubing (Figure 6-33). -
Page 224: Pta Calibrant Vial
1. Shut down the system as described in Section 6.1.1, Shutting Down the ThermaBeam Mass Detector. 2. Open the front door of the ThermaBeam Mass Detector and remove the safety panel (see Figure 6-11). 3. Turn the nut holding the calibrant vial counterclockwise to loosen the vial, then pull... -
Page 225: Rebuilding The Cold Cathode Vacuum Gauge
6. Reinstall the safety panel and shut the front door of the ThermaBeam Mass Detector. 7. Start up the ThermaBeam detector as described in Section 6.1.2, Starting Up the ThermaBeam Mass Detector. 8. Check the chamber pressure value in the Manual Tune window and make a note of the reading. -
Page 226: Removing The Cold Cathode Vacuum Gauge
Section 6.1.1, Shutting Down the ThermaBeam Mass Detector. 2. Use a screwdriver to remove the right side panel and the top rear panel of the ThermaBeam Mass Detector. Removing the Gauge 3. Open the swing clamp to remove the cold cathode vacuum gauge (Figure 6-35). -
Page 227: Cold Cathode Vacuum Gauge Components
7. Remove the C-ring with a pair of C-ring pliers to release the cathode cup and the electrode. Nickel C-Ring Striker Cathode Cup Plate Collar Electrode O-Ring Body Tube Magnet Housing TP01337 Figure 6-36 Cold Cathode Vacuum Gauge Components Cleaning the Gauge 8. - Page 228 22. Use the NW25 center ring and the swing clamp to reinstall the cold cathode vacuum gauge. 23. Reinstall the side panel and the top rear panel of the ThermaBeam Mass Detector. 24. Start up the ThermaBeam Detector as described in Section 6.1.2, Starting Up the...
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Page 229: Replacing The Ion Detector Assembly
To replace the ion detector: 1. Shut down the system as described in Section 6.1.1, Shutting Down the ThermaBeam Mass Detector. 2. Open the vacuum chamber lid after the system has vented. Note:... -
Page 230: Removing The Ion Detector
Screws on the TP01259 Detector Front Plate Figure 6-37 Removing the Ion Detector 4. Pull the detector straight out from the vacuum chamber. 5. Install a new detector. 6. Close the vacuum chamber lid and secure the latch. 7. Start up the system as described in Section 6.1.2, Starting Up the ThermaBeam Mass Detector. -
Page 231: Appendix A Specifications
Appendix A Specifications ™ This appendix contains the following types of specifications for the ThermaBeam Mass Detector: • Operational (Table A-1) • Mechanical (Table A-2) • Environmental (Table A-3) • Power (Table A-4) • Communications (Table A-5) • Regulatory compliance (Table A-6) Table A-1 Operational Specifications... -
Page 232: Mechanical Specifications
Chamber operating pressure range to 10 torr Table A-3 Environmental Specifications Condition Specification Temperature, ambient for ThermaBeam Mass 10 to 32 °C (50 to 90 °F) Detector and LC instruments Stable to ±2 °C Humidity 20 to 80%, noncondensing Exhaust handling requirements... -
Page 233: Power Specifications
Condition Specification Voltage requirement 100 to 240 Vac at 50/60 Hz Power requirement • ThermaBeam Mass Detector: 800 VA • 2690/2695 Separations Module: 950 VA (maximum) • Computer and monitor: 980 VA • Vacuum pumps and cold trap: see the manufacturer’s manuals... - Page 234 IEC 801-2, ESD Susceptibility IEC 801-3, Radiated Susceptibility - Electric Field IEC 801-4, ESD Susceptibility - Burst Interference Transients Note: The ThermaBeam Mass Detector is manufactured according to current Good ® Manufacturing Practices and the quality management system of Waters manufacturing facility in Milford, Massachusetts (U.S.A.) which complies with the International...
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Page 235: Spare Parts And System Tubing
This appendix lists the spare parts that are recommended for customer installation and describes the tubing that is included in the Integrity System. Parts not listed in this appendix require installation by a trained Waters service representative. B.1 Spare Parts Table B-1 lists spare parts and their part numbers. - Page 236 Table B-1 Spare Parts (Continued) Category Item Part Number ThermaBeam Mass Detector Capillary, Fused Silica WAT240789 58 µm × 360 µm × 1 m (Continued) Capillary, Fused Silica WAT240788 51 µm × 360 µm × 1 m Capillary Fittings Kit...
- Page 237 Table B-1 Spare Parts (Continued) Category Item Part Number Vacuum System (Continued) Tool, Turbo Pump Spanner WAT241110 Vacuum Pump Oil, Case of 12, 1 L WAT241183 Viton Seal, MS Turbo Pump WAT240812 Miscellaneous 996/2996 MS Flow Cell WAT057462 Alkylphenone Standard Kit WAT241190 Caffeine Standard Kit WAT241140...
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Page 238: Integrity System Tubing
Waste Low Volume Module Flow Cell Vent Transducer In-line Sample Filter Loop ThermaBeam Mass Detector Injector In-line 25 µl N e b u l i z e r Filter Syringe A s s e m b l y Seal Union... - Page 239 Table B-2 Integrity System Tubing (Continued) Length Number Description (inches) (inches) Assy, Tube, Out Right Head, In Left Head, TFE 0.040 Assy, Tube, Wash Pump Out, In Right Head, TFE 0.040 23.5 Assy, Tube, Reservoir to Pump Inlet, TFE 0.040 29.0 Assy, Tube, Left Head Outlet to Reservoir, TFE 0.040...
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Page 240: Index
AC power supply See also Fluidic connections connecting power cord correcting air leak controlling vent valve installing AC safety interlocks Contacting Waters Technical Service Air ballast. See Gas ballast control knob Controller printed circuit board Air leaks Conventions, documentation determining size fixing... - Page 241 power on Expansion region user-initiated cleaning Diagnostic tools removing front panel indicator lights Extractor lens malfunction state cleaning overview description startup tests system status Diverter valve 996/2996 PDA Detector connection Filament auto-waste setting powering on after maintenance description protecting nebulizer connection replacing waste line connection switching to backup...
- Page 242 Gas ballast/oil return assembly status connecting to oil mist filter Inline restrictor description oil return tubing component function Installation installing on vacuum pumps helium requirements making fluidic connections required materials vacuum pumps Integrity System Hardware control conceptual view Helium configuration changing supply connecting the IEEE-488 cables connecting to inlet port...
- Page 243 Isolation valves cleaning ionizer components connecting to Stage 1 pump cleaning LC/MS interface connecting to Stage 2 pump cleaning Pirani gauge description cleaning quadrupole mass filter opening and shutting defrosting cold trap preventing overpressure overview rebuilding cold cathode vacuum gauge refilling calibrant vial replacing a filament LC flow...
- Page 244 Mobile phases compatibility with Integrity system handling Particle beam technology Mode selector control knob, setting high Perfluorotributylamine. See PTA vacuum Pirani gauge Momentum separator cleaning cleaning connecting removing connecting the vacuum pump signal MS OK cable connecting cable to the 600S Controller description measuring interface pressure output signal, stopping the LC flow...
- Page 245 Temperatures Site requirements compartment electrical controlling in Manual Tune window environmental nebulizer exhaust Test equipment, connecting vacuum pumps ThermaBeam Mass Detector Solvents features compatibility with Integrity System front view venting and trapping inspecting Specifications internal components Stage 1 pump internal electronics...
- Page 246 troubleshooting, overview tuning unpacking User-initiated diagnostic tests, description vacuum required Thermal pneumatic nebulization, definition Thermal pneumatic nebulizer. See Nebulizer TMD Communications Error dialog box Vacuum TMD compartment temperature failure, caused by bad vacuum pump malfunction state inadequate in chamber TMD Diagnostics dialog box setting high Troubleshooting Vacuum chamber...
- Page 247 Voltage, vacuum pump changing checking Voltages changing in Manual Tune window vacuum pump requirements verifying on vacuum pumps Waste line, connecting to divert valve Water adsorption, in vacuum system Waters Technical Service Index 247...
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