Calibration Failed - Agilent Technologies 1260 Infinity User Manual

Calibration Failed

The intensity maximum was not found during wavelength calibration.
Calibration 0 Failed: Zero-order calibration failed.
Calibration 1 Failed: 656 nm calibration failed.
During zero-order and 656 nm calibration, the detector searches for the
intensity maximum. If the maximum is not detected within the scan range, the
error message is generated.
Probable cause
1 Lamp is OFF.
2 Incorrect flow cell installation.
3 Flow cell contamination or air bubbles.
4 Intensity too low.
5 Current step value too far from maximum.
6 Misaligned/defective grating assembly.
7 ProbableCause?
Agilent 1260 Infinity Variable Wavelength Detector User Manual
Error Information
Suggested actions
Switch on the lamp.
Ensure the flow cell are installed correctly.
Clean/replace flow cell windows or remove air
bubbles.
Replace lamp.
Enter a different calibration step value (different
scan range). Repeat the calibration.
Run the grating-motor test to determine if the
grating assembly is defective. If defective,
replace the optical unit.
Exchange the VWM board.
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