Quantitative Analysis And Spectrum Measurement; Quantitative Analysis Introduction; Quantitative Analysis Program Overview; Quantitative Analysis Program - Jasco V-530 Instruction Manual

3. Quantitative Analysis and Spectrum Measurement

T hi s sect i on descri bes quant i t at i ve anal ysi s and spect rum m easurem ent . T he param et ers are descri bed
onl y bri ef l y i n order t o cl ari f y t he operat i on f l ow . F ol l ow t he procedures out l i ned bel ow i n order t o becom e
f am i l i ar w i t h t he operat i on of V -500W . F or m ore det ai l ed i nf orm at i on, see t he sect i on f or each program .
3. 1 Q uant i t a t i v e A nal y si s I n t r oduct i o n
T he f ol l ow i ng sect i ons bri ef l y descri be t he quant i t at i ve anal ysi s program and i t s operat i on f l ow , f ol l ow ed by
t he procedure f or creat i ng cal i brat i on curves, unknow n sam pl e m easurem ent , and savi ng and pri nt i ng
resul t s.
3. 1. 1 Q uant i t at i ve anal ysi s program overvi ew

3.1.1.1 Quantitative analysis program

T he quant i t at i ve anal ysi s program has t he f ol l ow i ng f eat ures.
(1) T hree m et hods of anal ysi s out l i ned.
1) 1-w avel engt h quant i t at i ve anal ysi s. F i g. 3. 1 (1).
U sed f or norm al sol ut i on sam pl e.
2) 2-w avel engt h quant i t at i ve anal ysi s. F i g. 3. 1 (2).
U sed f or basel i ne correct i on.
3) 3-w avel engt h quant i t at i ve anal ysi s. F i g. 3. 1 (3).
U sed f or basel i ne correct i on.
T he f ol l ow i ng f orm ul a i s used f or 3-w avel engt h quant i t at i ve anal ysi s. W L1 i s t he w avel engt h and E
(1) t he absorbance at t hat w avel engt h.
WL 1
(Peak)
(1)
1-wavelength
(2)
T he cal i brat i on curve can be sel ect ed f rom t he m odes show n bel ow , accordi ng t o t he appl i cat i on.
-
| WL 1
DAbs = -
E ( ) 1
WL 2 WL 1
(Base 1) (Peak)
(2)
2-wavelength
F i gure 3. 1 Q uant i t at i ve anal ysi s m et hods
× + -
WL 2 | E ( ) | 3 WL 3 WL
-
| WL 3 WL 2 |
D Abs
E(2)
WL 2
(Base 1)
10
×
1 | E ( ) 2
E(1)
E(3) D Abs
WL 1 WL 3
(Peak) (Base2)
(3)
3-wavelength