Endress+Hauser analytikjena Biometra TOne Operating Manual
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Endress+Hauser analytikjena Biometra TOne Operating Manual

High-performance thermal cycler for dna amplification by pcr
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Operating Manual
Biometra TOne
High-performance thermal cycler for
DNA amplification by PCR

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Summary of Contents for Endress+Hauser analytikjena Biometra TOne

  • Page 1 Operating Manual Biometra TOne High-performance thermal cycler for DNA amplification by PCR...
  • Page 2 Biometra GmbH (Part of Analytik Jena GmbH) Manufacturer Rudolf-Wissell-Str. 30 37079 Göttingen  Germany Phone + 49 551 50 68 6-0 + 49 551 50 68 6-66 Email Info@analytik-jena.com Biometra GmbH Service Rudolf-Wissell-Str. 15 37079 Göttingen  Germany Phone + 49 3641 777407 Email Service@analytik-jena.com http://www.analytik-jena.com...

  • Page 3: Table Of Contents

    Biometra TOne Contents Contents Basic information ....................... 7 Notes on the operating manual ..................7 Purpose ..........................7 Safety and warning instructions ................9 General notes ........................9 Safety markings on the Biometra TOne ................9 Technical condition ......................10 Requirements for the operating personnel ..............
  • Page 4 Contents Biometra TOne Block status ........................33 Quick start function ......................34 Incubation mode ......................35 Creating, editing and saving programs ..............37 Programming modes ....................... 37 Creating a new program/using a program template ............. 38 Assigning program names ....................40 Setting the heated lid temperature ................
  • Page 5 Biometra TOne Contents 10.6 Backup ..........................79 10.7 Cycler Info ........................80 10.8 Contact..........................81 Adaptation of programs ................... 83 Quick reference guide ....................85 Troubleshooting ....................... 90 13.1 Administrator password forgotten ................. 90 13.2 Slow heating and cooling ....................90 13.3 Auto restart ........................
  • Page 6 Figures Biometra TOne Fig. 23 Temperature gradient on a 96 well sample block............45 Fig. 24 Linear Gradient Tool screen .................... 46 Fig. 25 Standard gradient programming screen ................. 47 Fig. 26 Biometra TOne spreadsheet program view ..............47 Fig. 27 Gaphical program view....................

  • Page 7: Basic Information

    Biometra TOne Basic information Basic information Notes on the operating manual The Biometra TOne is intended for operation by qualified specialist personnel observing this operating manual. The operating manual contains information about the design and operation of the Bi- ometra TOne and provides personnel familiar with PCR technology with the necessary know-how for the safe handling of the equipment and its components.
  • Page 8 Basic information Biometra TOne information and program parameters can be entered directly on the screen. Due to the high ramp rates, the TOne thermal cycler is suitable for fast PCR applications, thereby helping to shorten program run times.

  • Page 9: Safety And Warning Instructions

    Biometra TOne Safety and warning instructions Safety and warning instructions General notes For your own safety and to ensure error-free and safe operation of the Biometra TOne, please read this section carefully before commissioning. Besides the safety instructions in this user manual and the local safety regulations that apply to the operation of the device, the general applicable regulations regarding acci- dent prevention, occupational health and safety and environmental protection have to be observed and complied with.

  • Page 10: Technical Condition

    Safety and warning instructions Biometra TOne Keep ventilation slits clear Keep the ventilation slits on the underside and at the rear of the device clear. Make sure that there are no objects underneath the device (e.g., paper) that may be sucked against the ventilation slot and thus inter- fere with ventilation.

  • Page 11: Safety Instructions: During Operation

    Biometra TOne Safety and warning instructions Safety instructions: during operation 2.6.1 General The operator of the Biometra TOne must make sure before each commissioning that the condition of the device including the safety equipment is sound. The following has to be observed: ...

  • Page 12: Handling Of Samples, Auxiliary And Operating Materials

    Safety and warning instructions Biometra TOne  The electrical components must be checked regularly by a qualified electrician. Any defects, such as loose connections, faulty or damaged cables, must be repaired with- out delay.  Before opening the device it must be switched off at the power switch and the mains plug must be disconnected from the mains outlet! ...

  • Page 13: Behavior During Emergencies

    Biometra TOne Safety and warning instructions  Only use original spare parts, wear parts and consumables. These have been tested and guarantee safe operation. Behavior during emergencies In case of danger or accidents, immediately switch off the Biometra TOne using the main switch on the equipment backplate.

  • Page 14: Function And Setup

    Function and setup Biometra TOne Function and setup The Biometra TOne thermal cycler is available in the following models:  Biometra TOne 96 with aluminum block  Biometra TOne 96G with aluminum block and gradient function The device is controlled by a 7' ' touchscreen display and an easy-to-use user interface. PCR protocols and run log files can be saved to a connected USB stick.

  • Page 15: High Performance Smart Lid

    Biometra TOne Function and setup prevent condensed water from penetrating into the Peltier elements underneath the sam- ple block and other parts of the electronics. The sealing protects the Peltier elements and prolongs the lifetime of the device. High Performance Smart Lid The Biometra TOne heated lid has been optimized to heat twice as fast as with older models and thus helps to shorten protocol run times.

  • Page 16: Easy Programming

    Function and setup Biometra TOne Easy programming Creating new PCR programs takes a lot of time if for each single step all parameters have to be set manually. The Biometra TOne software offers several pre-installed program templates for different applications. The program templates provide a general protocol structure that can be easily adapted for the current experiment.

  • Page 17: Order Numbers

    Biometra TOne Function and setup used for manual hotstart applications. After inserting the samples into the heating block, the PCR protocol can be started just by pressing "continue". Order numbers Instruments incl. block and base unit Model Block type Gradient range Order number TOne 96 Aluminum...

  • Page 18: Installation

    Installation Biometra TOne Installation Scope of delivery 1. Thermal cycler 2. Mains cable 3. Operating instructions 4. Quick reference guide Please keep the original packaging in case the device needs to be returned. The shipping box for the Biometra TOne thermal cycler contains a specially developed foam system for the transport of high quality electronic devices.

  • Page 19: Commissioning

    Biometra TOne Installation Commissioning 4.4.1 General setup WARNING Danger of electric shock! Prior to commissioning, please make sure that the operating voltage set on the device is the same as the mains voltage (see section "Setting the oper- ating voltage" p. 18) NOTICE Do not cover the ventilation slits! Ensure that the ventilation slits are clear.
  • Page 20 Installation Biometra TOne  For an alternative direct connection of the thermal cycler, the thermal cycler must be changed to a network connection with a fixed IP (e.g. IP 192.168.0.30, subnet mask 255.255.255.0) (see section "Network" p. 65). The network card must also be changed to a fixed IP(V4) via the network adapter settings under Windows (e.g.

  • Page 21: Control Elements

    Biometra TOne Control elements Control elements Biometra TOne front view Dial for adjusting the contact pressure Lid closing button Display USB port Fig. 3 Front view of the Biometra TOne Biometra TOne rear view The Ethernet port, mains plug and mains switch are located at the rear of the Biometra TOne.

  • Page 22: High Performance Smart Lid (Hpsl)

    Control elements Biometra TOne Ethernet port Mains switch Fuse holder Mains plug Fig. 4 Rear view of the Biometra TOne High Performance Smart Lid (HPSL) The Biometra TOne has a height-adjustable lid for optimal contact pressure on the sam- ples. 5.3.1 Closing the lid 1.

  • Page 23: Opening The Lid

    Biometra TOne Control elements Fig. 5 Placement of sample tubes in the block 5.3.2 Opening the lid NOTICE Never open the lid under pressure! Always follow the sequence below when opening the lid. If the lid is opened under pres- sure, the locking mechanism may be damaged.

  • Page 24: Keypad

    Control elements Biometra TOne Fig. 6 Keyboard The keyboard offers the following special keys: Space Shift Delete single character Delete all characters Back Forward Note: For some functions the maximum number of characters is limited. Usernames, passwords and program names can have a maximum of 13 characters and user initials a maximum of 3 characters.

  • Page 25: Cursor Keys

    Biometra TOne Control elements The keypad can be used to enter digits, dots, minus signs and infinite. In addition the following special keys are available: Delete single character Delete all characters Infinite 5.4.3 Cursor keys The Biometra TOne software uses lists, tables or graphical representations. Whenever the number of entries becomes higher than the maximum number that can be displayed on a screen, the software will show cursor keys for navigation.
  • Page 26 Control elements Biometra TOne Left Down Confirm Cancel Login User management New user Delete user Edit user Incubation Start incubation Programs New program Open template Copy program/copy all programs Delete program Edit program Start program Quick select program or user...
  • Page 27 Biometra TOne Control elements Stop program Done Save program Pause program Continue program Skip step Graphical view Spreadsheet view Gradient display Edit step Insert step Delete step Temperature incre- ment Time increment Heating rate Gradient Linear Gradient Tool Standard gradient programming Preheating OFF...
  • Page 28 Control elements Biometra TOne Preheating ON Tools Settings Date and time Automatic user lo- gout Beeper Network Display brightness Display darker Display brighter Touchscreen calibra- tion Factory settings Documentation Run log file Run log file overview Save run log file to Save all run log files to USB View run log file...
  • Page 29 Biometra TOne Control elements Log file self-test Error log file Extended self-test Start extended self- test Stop extended self- test Backup file Save backup file Load backup file Cycler information...

  • Page 30: Operation

    Operation Biometra TOne Operation Power on self-test After the Biometra TOne is switched on, the device performs an automatic self-test (power on self-test). During the test the device shows the following screen: Fig. 8 Start screen For the power on self-test a log file is created and stored in the thermal cycler memory (see section "Power On Logfile"...

  • Page 31: Login

    Biometra TOne Operation Fig. 9 Login screen Login After starting the Biometra TOne, the login screen is displayed. To log in as an existing user, press Login. Login In the next screen select a user by pressing the corresponding button. Fig.

  • Page 32: Home Screen

    Operation Biometra TOne Note: The position of the buttons can change. The button for the most recently logged in user is always shown in the first position at the top left of the screen. All the other buttons become arranged from top left to bottom right according to the date and time of the latest user login.

  • Page 33: Block Status

    Biometra TOne Operation Command Function Section Programs Viewing, running and editing programs See section "Creating, editing and saving programs" p. 37 Block Shows the current block status. If a block is free, a See section "Block status" p. 33 and section "Quick touch on the button leads to a list of previously used or start function"...

  • Page 34: Quick Start Function

    Operation Biometra TOne Running Yellow Program view Pause Blue Program view Quick start function The Biometra TOne software offers a quick start function for previously used programs. To use the quick start function, press the block status display button in the login screen (see section "Login screen"...

  • Page 35: Incubation Mode

    Biometra TOne Operation Fig. 14 Biometra TOne program quick start screen Select the program for quick start in the table and press the Start button to start the se- lected program. If necessary, the program can be edited before the start or a new program can be created based on a template (see section "Programming modes"...

  • Page 36: Fig. 15 Biometra Tone Incubation Mode Screen

    Operation Biometra TOne Note: To set the time to endless, use the button. To start incubation, press the Start button. Start Fig. 15 Biometra TOne incubation mode screen During incubation the following commands can be used: Stop Pause Continue Skip Note: To stop the incubation, use the corresponding command.

  • Page 37: Creating, Editing And Saving Programs

    Biometra TOne Creating, editing and saving programs Creating, editing and saving programs Programming modes The Biometra TOne software offers spreadsheet or graphical programming. Use the but- tons to toggle between the two modes. The following screenshots show the spreadsheet and graphical programming mode. Graphical view Spreadsheet view Fig.

  • Page 38: Creating A New Program/Using A Program Template

    Creating, editing and saving programs Biometra TOne Both programming modes show the same information but in different ways. In the spreadsheet mode all parameters are summarized in a table. In the graphical mode sym- bols are displayed for some parameters (see the table below). Toggle to the spreadsheet mode to view the settings for these parameters.

  • Page 39: Fig. 18 Program Overview Screen

    Biometra TOne Creating, editing and saving programs Fig. 18 Program overview screen 3. In the program overview screen, press the New from template button: New from template 4. The program template screen opens (see Fig. 20). Search for a program template using the cursor keys and press the corresponding button to select it.

  • Page 40: Assigning Program Names

    Creating, editing and saving programs Biometra TOne spreadsheet form. By using the program preview function, templates can be checked be- fore they are opened. 5. To open a program template, press the Open template button. Open template Fig. 20 Program template screen Assigning program names Each template has a specific name which is superimposed in the programming screen.

  • Page 41: Preheating The Heated Lid

    Biometra TOne Creating, editing and saving programs Fig. 21 Heated lid temperature setting screen NOTICE The heated lid automatically switches off at a temperature difference of >75 °C between the block and the heated lid. At these low block temperatures, sample condensation on the tube lid is no longer to be expected.

  • Page 42: Editing All Parameters Of A Step

    Creating, editing and saving programs Biometra TOne Parameter Description and limits Section Temperature Temperature for a step in °C. The target temperature can be set be- See section "Editing all pa- tween 3.0°C and 99.0°C in tenths of a degree. rameters of a step"...

  • Page 43: Programming Loops

    Biometra TOne Creating, editing and saving programs Fig. 22 Pprogram step editing screen Note: To program a step at least the temperature and the hold time must be defined. To set the temperature, touch the corresponding input field and enter a value between 3.0°C and 99.0°C.

  • Page 44: Programming A Temperature Increment

    Creating, editing and saving programs Biometra TOne To program a loop, select the last step of the loop and enter the step number to which the program should return in the Go To input field (see Fig. 22). To define the number of repetitions, enter a number in the Cycles field (see Fig.

  • Page 45: Adjusting The Ramp Rate

    Biometra TOne Creating, editing and saving programs 7.6.1.4 Adjusting the ramp rate The average ramp rate can be adjusted for each step. This adjustment can be useful if PCR programs are transferred from slower devices to the Biometra TOne or for special PCR applications, for example, telomerase PCR.

  • Page 46: Fig. 24 Linear Gradient Tool Screen

    Creating, editing and saving programs Biometra TOne Sample replicates are distributed across the columns of the sample block and tested for the temperature giving the optimal result. To program a gradient step press the Grad button. Grad There are two options to program a gradient. To toggle between the two options, press the corresponding button on the Biometra TOne gradient programming screen (see Fig.

  • Page 47: Direct Programming Of Program Parameters For A Step

    Biometra TOne Creating, editing and saving programs effective temperatures for all the other columns are automatically calculated and dis- played as a bar chart. Fig. 25 Standard gradient programming screen 7.6.2 Direct programming of program parameters for a step The Biometra TOne software allows individual parameters (temperature, hold time, loops, temperature and time increment, as well as the ramp rate) for a step to be edited directly in the graphical or spreadsheet view.

  • Page 48: Fig. 27 Gaphical Program View

    Creating, editing and saving programs Biometra TOne ② Hold time h:mm:ss ③ Loops ④ Number of cycles ⑤ Temperature increment ∆T (°C) ⑥ Time increment ∆t (s) ⑦ Ramp rate ∆R (°C/s) To edit program parameters in the graphical view, press the corresponding input field for the parameter that you want to edit for the step (see Fig.

  • Page 49: Fig. 28 Individual Program Parameter Editing

    Biometra TOne Creating, editing and saving programs This window allows you to enter the desired value for the parameter using the keyboard. Fig. 28 Individual program parameter editing If the sample block is gradient-enabled, you also have the option of programming a gra- dient step.

  • Page 50: Inserting A Step

    Creating, editing and saving programs Biometra TOne Inserting a step To insert a step, select a program step on the Biometra TOne spreadsheet or graphical programming screen and press the Insert button. Insert When you press the Insert button, the screen for editing program steps opens. Enter the desired parameters for the new step.

  • Page 51: Fig. 30 Program Overview Screen

    Biometra TOne Creating, editing and saving programs Note: The user directory of the logged in user is automatically preselected. Note: If a new program is to be saved, the first free storage location in the directory of the currently logged in user is automatically selected. However, the storage location can be changed using the cursor keys above and below the lists.

  • Page 52: Fig. 31 Quick Select Screen

    Creating, editing and saving programs Biometra TOne Fig. 31 Quick select screen Before saving the program there is still the possibility of changing the program name. To do this, press the Save as button and enter the new program name using the key- board.

  • Page 53: Starting, Coping And Deleting Programs

    Biometra TOne Starting, coping and deleting programs Starting, coping and deleting programs Starting a program The Biometra TOne software offers a quick start function for previously started pro- grams (see section "Quick start function" p. 34). This section describes the process of selecting and starting stored programs from a user account.

  • Page 54: Copying A Program

    Starting, coping and deleting programs Biometra TOne Fig. 33 Biometra TOne program overview screen with preview Copying a program To copy a program, touch the corresponding buttons to select a user directory and a pro- gram on the Biometra TOne program overview screen (see Fig. 32). If the number of user directories or programs is higher than the maximum number that can be displayed in the list, the buttons above and below the lists can be used to scroll up and down.

  • Page 55: Copying All Programs

    Biometra TOne Starting, coping and deleting programs Fig. 34 Program overview screen Copying all programs To copy all programs, touch the corresponding button to select a user directory on the Biometra TOne program overview screen (see Fig. 32). If the number of user directories or programs is higher than the maximum number that can be displayed in the list, the buttons above and below the lists can be used to scroll up and down.

  • Page 56: Deleting A Program

    Starting, coping and deleting programs Biometra TOne Fig. 35 Program overview screen Deleting a program To delete a program, touch the corresponding buttons to select a user directory and a program on the Biometra TOne program overview screen (see Fig. 32). If the number of user directories or programs is higher than the maximum number that can be displayed in the list, the buttons above and below the lists can be used to scroll up and down.

  • Page 57: Deleting All Programs

    Biometra TOne Starting, coping and deleting programs Deleting all programs To delete all programs, touch the corresponding buttons to select a user directory on the Biometra TOne program overview screen (see Fig. 32). If the number of user directories is higher than the maximum number that can be displayed in the list, the buttons above and below the lists can be used to scroll up and down.

  • Page 58: Running, Pausing, Continuing And Stopping Programs

    Running, pausing, continuing and stopping programs Biometra TOne Running, pausing, continuing and stopping programs Display during a run After a program has been started (see section "Starting a program" p. 53) the Biometra TOne software can display the currently running program in a spreadsheet or graphical view.

  • Page 59: Pausing A Program

    Biometra TOne Running, pausing, continuing and stopping programs Gradient view Fig. 38 Displays during the run in a graphical, spreadsheet and gradient view The following parameters are summarized in the headline in the table:  Program name  Sample block temperature ...

  • Page 60: Fig. 39 Run Display Screens During A Program Pause

    Running, pausing, continuing and stopping programs Biometra TOne Pause The word Pause is shown and the pause time is counted in the Remain.Time field. Spreadsheet view Graphical view Fig. 39 Run display screens during a program pause Note: Pressing the Pause button requires manual interaction. The Biometra TOne soft- ware also allows pauses to be programmed (see section "Editing steps"...

  • Page 61: Continuing The Program

    Biometra TOne Running, pausing, continuing and stopping programs Continuing the program To continue a paused program, press the Continue button on the TOne run display screen (see Fig. 39). Continue The Biometra TOne will continue the program from exactly the point at which it was paused.

  • Page 62: Tools

    Tools Biometra TOne Tools The Biometra TOne software provides the following tools: Settings Defines basic device settings See section "Configuration" p. 62 User Management Tool for user management See section "User management" p. 68 Documentation Access to stored log files See section "Documentation"...

  • Page 63: Setting The Date And Time

    Biometra TOne Tools Network Defines network settings See section "Network" p. 65 Display brightness Adjusts the display brightness See section "Display brightness" p. 66 Touch screen calibration Calibrates the touchscreen See section "Screen calibration" p. 67 Factory settings Resets all the settings See section "Factory settings"...

  • Page 64: Automatic User Logout

    Tools Biometra TOne Activate the corresponding input field and use the keypad on the right side of the date and time entry screen to enter digits. The following buttons may also be helpful when completing the fields: Delete single character Delete all characters Go to the next input field Note: The following buttons are inactive on the date and time entry screen and cannot be...

  • Page 65: Configuring The Beeper

    Biometra TOne Tools Note: The following buttons are inactive on the automatic user logout screen and can- not be used: 10.1.3 Configuring the beeper The Biometra TOne has a beeper which, if switched on, will issue an acoustic signal when a PCR program is finished. The beeper can be switched ON or OFF by activating the corresponding checkbox.

  • Page 66: Network Users

    Tools Biometra TOne management. If DHCP is activated, the device automatically receives the network config- uration settings. If the static checkbox is activated, you can enter the IP address, the subnet mask and the port number using the keypad on the right side of the screen. The following buttons may be helpful when completing the fields: Delete single character Delete all characters...

  • Page 67: Screen Calibration

    Biometra TOne Tools Fig. 47 Display brightness adjustment screen 10.1.6 Screen calibration To calibrate the screen, follow the instructions and touch the screen at the center of the displayed circle. This procedure is repeated three times at different positions on the dis- play.

  • Page 68: User Management

    Tools Biometra TOne Fig. 49 Confirmation prompt Using the factory settings tool will delete all users and programs! Use a backup file to restore the system (see section "Backup" p. 79). 10.2 User management The Biometra TOne can manage up to 90 user directories. User management allows new users to be created.

  • Page 69: Creating A User

    Biometra TOne Tools Down To select a user, press on the corresponding row in the table. 10.2.1 Creating a user To create a new user, press the New User button. New user In the next screen enter the  Username (up to 13 characters or numbers) ...

  • Page 70: Delete User

    Tools Biometra TOne Edit On the next screen the user name and the language setting can be changed. Fig. 52 User data screen Note: For the administrator "Admin" the user name and the initials "ADM" cannot be modified. It is recommended to change the password for the administrator "Admin" after the first system start and to create a backup of the system.

  • Page 71: Documentation

    Biometra TOne Tools After confirmation of the confirmation prompt the user and all of his programs will be deleted. 10.3 Documentation The Biometra TOne software provides the following documentation tools: Run Log File Documentation of PCR runs See section "Run log file" p. 71 Power On Log File Result of the latest initial self-test See section "Power On Logfile"...

  • Page 72: Fig. 55 Biometra Tone Run Log File Overview Screen

    Tools Biometra TOne Fig. 55 Biometra TOne run log file overview screen The run log files are sorted by date and time. The run log file for the program that was last started is shown at the first position in the table. The initials of the user who started the program, the number of the sample block being used, the program name, the direc- tory and the program number are also shown.

  • Page 73: Fig. 56 Biometra Tone Run Log File Screen

    Biometra TOne Tools  Sample block type  Block serial number  Block number  Messages Fig. 56 Biometra TOne run log file screen To view the corresponding program, press the View Program button. View program The Biometra TOne offers the option to show the program in graphical from or as a spreadsheet (see section "Programming modes"...

  • Page 74: Power On Logfile

    Tools Biometra TOne Fig. 57 Program view screen Use the buttons to toggle between the overview screen, the run log file screen and the view program screen. Overview Run Log Files View Run Log File Show Program To export all the saved run log files on USB, press the Save all Run Log Files on USB button.

  • Page 75: Extended Self-Test Log File

    Biometra TOne Tools Fig. 58 Power on log file screen 10.3.3 Extended self-test log file During the extended self-test the Biometra TOne checks several functions and compo- nents like cooler, thermal tracking, heating and cooling rate, refrigeration, gradient, heated lid and regulation (see section "Extended self-test" p. 76). Press on the correspond- ing button to see the results for a specific test summarized in spreadsheet form.

  • Page 76: Extended Self-Test

    Tools Biometra TOne Fig. 60 Biometra TOne error log file screen 10.4 Extended self-test NOTICE The Extended Self-Test must be performed under the following conditions to achieve re- producible and meaningful results:  Ambient temperature in the range 20 °C to 25 °C ...

  • Page 77: Fig. 61 How To Load The Sample Block With Plasticware For The Extended Self-Test

    Biometra TOne Tools Regulation Tests if the sample block is controlled properly. for gradient-capable models only The Biometra TOne software will automatically recommend at regular intervals to carry out an extended self-test. Although it is not absolutely necessary to perform the test, it is highly recommendable to follow the prompt and to let the device check itself.

  • Page 78: Service Info File (Sinf)

    Tools Biometra TOne The software provides a progress overview during the extended self-test. When a test has finished, the result "Passed" or "Failed" is displayed. For the currently running test, the status "Running" is shown and all other tests for which no result is shown are not fin- ished yet.

  • Page 79: Backup

    Biometra TOne Tools Fig. 64 Service info file creation screen To create a service info file, connect a USB stick to the Biometra TOne and press the Info File to USB button. Info File to USB The service info file can be sent by e-mail to the Biometra service department. For con- tact details, refer to the inside front cover.

  • Page 80: Cycler Info

    Tools Biometra TOne Fig. 65 Backup file screen Connect a USB stick to the device and press the Save backup file button to create a backup file. Save backup file To load a backup file, select it in the list and press the Load backup file button. Load backup file Backup files that are no longer used can be deleted using the functions: Delete all backup files...

  • Page 81: Contact

    Biometra TOne Tools  Revision number power board  Software version power board  Software version power board logic  Revision number processor board  Software version processor board  Revision number IO  Software version IO The information is summarized in two screens. Press the System/Cycler Info button to toggle between the two screens.

  • Page 82: Fig. 67 Service Department Contact Details Screen

    Tools Biometra TOne Fig. 67 Service department contact details screen...

  • Page 83: Adaptation Of Programs

    Biometra TOne Adaptation of programs Adaptation of programs If programs are exchanged between different thermal cycler models or the sample block is exchanged, programs might need to be adapted to the installed sample block type prior to the start or during editing. The following table provides an overview of possible pro- gram adaptations: Cause Example...
  • Page 84 Adaptation of programs Biometra TOne The heating and A program for a 96 well alu- The heating and cooling rate will be cooling rate is not minum block is to be started increased to the maximum possible reached. on a 96 silver block. value for the installed sample block type.

  • Page 85: Quick Reference Guide

    Biometra TOne Quick reference guide Quick reference guide WARNING Before initial operation, check the correct operating voltage is set on the voltage selec- tion switch on the underside of the device. Do not open the housing! Danger of electric shock! CAUTION The sample block and the heated lid can reach high temperatures during operation.
  • Page 86 Quick reference guide Biometra TOne  Press Incubate to incubate the sample block at a constant temperature.  Press Tools to access the system settings, user management, documentation, backup tool or self-test functions.  Press Logout to log out.  Press Programs on the TOne home screen The program overview screen opens: Editing, saving and copy- ing programs ...
  • Page 87 Biometra TOne Quick reference guide  Use the cursor keys above the keyboard to switch between program steps. The num- ber of the currently activated step is displayed on a blue background in the table at the top of the screen. ...
  • Page 88 Quick reference guide Biometra TOne heating and cooling rate) must be activated using the button to the left of the graph- ical display.  To insert a program step, select a step and press Insert. The screen for editing pro- gram steps opens.
  • Page 89 Biometra TOne Quick reference guide Spreadsheet view Graphical view  To pause an active program, press Pause. The color of the highlighted step changes to blue and the message Pause is shown in the Step field.  To continue a paused program, press Continue. ...

  • Page 90: Troubleshooting

    Troubleshooting Biometra TOne Troubleshooting 13.1 Administrator password forgotten If the administrator has forgotten his password, use the "Factory settings" function (see section "Factory settings" p. 67) to reset the system and download a backup file for re- covery (see section "Backup" p. 79). 13.2 Slow heating and cooling The Biometra TOne is equipped with strong fans that remove the heat from the heat sink.

  • Page 91: Adaptation Of Programs From Other Thermal Cyclers

    Biometra TOne Troubleshooting 13.5 Adaptation of programs from other thermal cyclers Since the Biometra TOne thermal cycler is a very fast device, it may be necessary to re- duce the heating and cooling ramp when running protocols from other (slower) thermal cyclers (see section "Adjusting the ramp rate"...

  • Page 92: Maintenance And Care

    Maintenance and care Biometra TOne Maintenance and care The Biometra TOne is mainly maintenance-free. The care and maintenance tasks which can be performed by the customer are limited to the cleaning and disinfection of the housing and sample block. All maintenance work and repairs beyond this scope must only be performed by Bi- ometra GmbH customer service personnel or authorized and trained persons.

  • Page 93: Disinfecting The Device

    Biometra TOne Maintenance and care 14.2 Disinfecting the device WARNING Biological hazard! Clean the Biometra TOne with particular care after analyzing poten- tially infectious material. Wear suitable protective equipment, e.g., protective gloves. NOTICE The only suitable cleaning method for the housing is wipe disinfection. When using spray disinfectants there is a risk that the liquid enters the sensitive elec- tronic system through the ventilation slits.

  • Page 94: Service

    Service Biometra TOne Service If you have any problems with the device, please contact our customer service depart- ment or your local Biometra dealer. For the address of the Biometra customer service department refer to the inside front cover of this operating manual. Please observe the return information (see section "Device return information"...

  • Page 95: Packing The Biometra Tone

    Biometra TOne Service 15.2 Packing the Biometra TOne Biometra uses an extra designed packaging system with inserts of foamed material. Important: The thermal cycler is only protected from transport damage if the original packaging is used and the packing instructions are followed. Biometra is not liable for transport damage due to incorrect packaging.

  • Page 96: Disposal

    Disposal Biometra TOne Disposal The operator of the Biometra TOne must dispose of any waste materials that occur dur- ing measurements (sample materials) in accordance with statutory and local regula- tions. At the end of its service life, the Biometra TOne and all its electronic components must be disposed of as electronic waste in accordance with valid regulations.

  • Page 97: Technical Specifications

    Biometra TOne Technical specifications Technical specifications Order number 846-x-070-311 846-x-070-301 Name Biometra TOne 96 Biometra TOne 96G Capacity 96 x 0.2 ml tubes, 96 well micro plates or 8 well strips Block material Aluminum Block coating Special alloy Max. heating rate* 4.0 °C/sec Max.
  • Page 98 Technical specifications Biometra TOne Weight 11.5 kg * measured inside the block The network cable must be at least performance class Cat 5e and the cable configuration has to be STP x = 2 for 230 V, 4 for 115 V, 5 for 100 V...

  • Page 99: Declaration Of Conformity

    Biometra TOne Declaration of conformity Declaration of conformity...

  • Page 100: Index

    Index Biometra TOne Index Auto restart 89 Personnel 10 Backup 79 Power on self-test 30 Block status 33 Program Care 91 Continue 61 Cleaning the housing 91 Copy 54 Configuration 62 Copy all 55 Decontamination 92 Delete 56 Disinfection instructions 92 Delete all 57 Display Gradient 45...
  • Page 101 Biometra TOne Index Date and time 63 Cycler Info 80 Display brightness 66 Extended self-test 76 Factory settings 67 Service info file 78 Network 65 Touch screen Screen calibration 67 Calibration 67 User management 68 User management Step Creating a user 69 Skip 61 Delete user 70 Tools 62...

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