Nikon ECLIPSE Ti2-U Instruction Manual page 72

Chapter 3 Usage of Components
Attaching a polarizer and adjusting the azimuth (to form Crossed Nicols)
1. Place the polarizer on the condenser mount.
Do not tighten the clamp screw at this stage.
2. Remove the DIC condenser module and the
DIC slider from the optical path.
3. Fully open the aperture diaphragm of the
condenser.
4. Focus on the specimen, and move the stage
to move the field of view to the portion of the
specimen where nothing is visible (or the
portion of a prepared specimen where a
cover glass is placed).
5. Place the analyzer and polarizer into the
optical path.
6. Return the rotational part of the polarizer to
the reference position.
Aligning the white indicator on the polarizer with
the center of the graduations, tighten the
rotational part clamp screw.
Confirm that the steel ball on the tip of the clamp
screw is in the groove on the rotational part.
7. Tighten the clamp screw to fix the rotational
part in place.
8. Turn the entire polarizer by hand so that the
white index mark that was fixed in place in
step 6 is lined up with the index mark on the
condenser holder.
9. Remove one of the eyepieces and attach the
centering telescope.
10. Turn the entire polarizer until a dark cross
appears.
11. With the dark cross in view, tighten the
clamp screw using a hexagonal screwdriver
so that the polarizer is fixed in place.
12. Replace the centering telescope with the
removed eyepiece.
If no dark cross is observed
• The higher the magnification, the easier the dark cross (Crossed Nicols) is to distinguish.
If it is difficult to see a dark cross with a low-magnification objective, replace it with a high-
er-magnification objective and retry.
• A dark cross may not be visible with some objectives.
In this case, remove the centering telescope and reinstall the removed eyepiece, increase the
illumination, and then observe the specimen plane. Turn the polarizer, and tighten the clamp screw at
the position where the field is the darkest.
The condenser turret can be turned by removing the positioning pin on the condenser turret using a
flathead screwdriver. By doing this, the azimuth can be fine-tuned by turning the condenser and
polarizer at the same time.
Attaching a polarizer
White index
mark
Scale plate
Index mark on the
condenser holder
Adjusting and fixing the polarizer
Dark cross on the pupil plane
52
Rotational part clamp
screw
Polarizer
rotation
lever
Clamp
screw