Nikon ECLIPSE Ti2-U Instruction Manual page 69

Inverted research microscope
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NAMC condenser slit image and slit diaphragm adjustment
In the NAMC condenser module, there are two
apertures (slit diaphragms) indicated by (1) and (2) in
the right figure, and a space called "bridge" indicated
by (3) between the apertures.
A polarizing plate is in aperture (2) and the
brightness of (2) is changed by changing the azimuth
of the polarizer.
Slit adjustment
Observe the slit image using the centering telescope.
Adjust the orientation of the slit diaphragm by turning
the NAMC condenser module so that apertures (1)
and (2) are parallel to gray zone (G) of the
modulator.
Adjust the position of the slit to cause the portion of
the slit aperture image (1), which does not change its
brightness by moving the polarizer, to overlap with
the gray zone (G).
 Operation:
Module adjustment
Turn the centering screw.
Turn the module through
the module rotation slot.
Fine adjustment of the NAMC condenser slit diaphragms
Images obtained by NAMC microscopy vary depending on the slit diaphragm adjustment of the NAMC
condenser module.
Emphasizing on 3D effects:
Align the inner side plane (A) of the objective's gray
zone (G) with the inner plane of aperture (1).
Reducing the contrast degradation due to
leakage light:
Align the inner side plane (A) of the objective's gray zone (G)
with the center of the bridge section (3).
Polarizing plate
The position of the slit
diaphragm is changed.
The orientation of the slit
diaphragm is changed.
(2)
Objective pupil
(3) Bridge section
Slit diaphragm image
Centering screw
Slit diaphragm adjustment
(2)
Objective pupil
(3) Bridge section
(2)
Objective pupil
(3) Bridge section
49
Chapter 3 Usage of Components
(1)
Dark region (D)
Gray region (G)
Centering screw
Module rotation slot
(A)
(1)
Dark region (D)
Gray region (G)
(A)
(1)
Dark region (D)
Gray region (G)

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