Nikon ECLIPSE Ti2-U Instruction Manual page 113

Combination of IMSI objectives and DIC prisms (condenser side/objective side)
To perform IMSI microscopy, it is necessary to use a correct combination of an IMSI objective, and DIC
prisms (condenser side/objective side). Referring to the following table, check the correct combination of the
objective, the condenser module and the DIC slider, and then place the optical components of the correct
combination in the optical path.
In addition to standard combinations, there are combinations focusing on high contrast and resolution. Select
the best combination as required.
Objective
Plan Apo VC 100X Oil
Plan LWD IMSI 100XC
Description in the parentheses in the DIC condenser module column indicates the color of the identification label supplied with each module.
Note on performing BF microscopy using IMSI objectives
When performing BF microscopy by using an IMSI objective, remove the DIC slider from the optical
path, and then place the polarizer slider and the condenser turret at hollow positions.
Combination of IMSI objectives and DIC prisms
Applica-
ble
WD
correction
(mm)
collar
Condenser
(mm)
IMSI N2 Dry
0.13 None
background +
black text)
IMSI N2 Dry
0.95 0.6-1.3
background +
black text)
LWD condenser lens (white text)
Standard combination
(Focus on the contrast)
DIC slider
module
(white
100x I
(white
100x III
93
Chapter 4 Microscopy Techniques
Focus on the resolution
Condenser
DIC slider
module
IMSI NR Dry
(white
100x I-R
background +
black text)