Nikon ECLIPSE Ti2-U Instruction Manual page 105

Ph objective
DLL
Generally, objects with a
DL
larger phase contrast
look dark.
Dark Therefore a dark image
contrast appears in a relatively
bright field, which is
similar to an image in BF
microscopy.
DM
Generally, objects with
larger phase contrast
look brighter.
Bright Therefore a bright image
BM
contrast appears in a relatively
dark field, which is
similar to an image in
DF microscopy.
Generally, objects with a
larger phase contrast
look darker.
ADL Therefore a dark image
appears in a relatively
bright field, which is
Dark
similar to an image in BF
contrast
microscopy.
(apodiza-
As compared with an
tion)
ordinary objective for
dark contrast, these
ADH
objectives can enhance
the contrast of a fine
structure by reducing
halo.
Selection of a proper Ph objective according to use
Plan Fluor Ph objectives can also be used for BF microscopy, DIC microscopy, and Epi-FL microscopy.
Plan Apochromat Ph objectives can also be used for BF microscopy, DIC microscopy, and Epi-FL
microscopy (excluding UV excitation).
However, both of these objectives have a built-in phase ring (phase plate ring) and thus image view might
be different from that with an objective dedicated to each relevant microscopy. When accurate microscopy
is required, use the objective dedicated to each microscopy.
Ph module (equivalent to the annular diaphragm in the figure "Optical path diagram for Ph
microscopy")
The Ph module is a ring-shape diaphragm that is
attached to the condenser turret.
An ID code called "Ph code" is attached to each of
the Ph objectives and Ph modules. There are five
codes, PhL and Ph1 to Ph4, depending on the size
of the phase ring and annular diaphragm. To perform
phase contrast microscopy, it is necessary to place a
Ph objective and a Ph module having the same Ph
code (Ph codes are unrelated to the magnifications
of the objectives) in the optical path.
Make sure to use a combination of a Ph objective
and a Ph module having the same code; otherwise
the phase contrast effect is not delivered.
Characteristics of Ph objectives
View Contrast
This is
suitable for
details
observation
with a focus
on micro
contrast.
This microscopy is suitable
for observing the form of and
detecting and calculating
minute fibers or granules
with a focus on macro
contrast.
This is
suitable for
details
observation
with a focus
on micro
contrast.
Latitude
Phase contrast
and absorbing
Half tone
objects (stained
(the use
objects) in the
range is wide)
low and mid
ranges
Hard tone
(the use Transparent
range is object in the low
relatively range
narrow)
Almost all ranges
Phase contrast
and absorbing
Half tone
objects (stained
(the use
objects) in the
range is wide)
low and mid
ranges
Hard tone
(the use Transparent
range is object in the low
relatively range
narrow)
Example of the Ph module
85
Chapter 4 Microscopy Techniques
Application examples
Spores of fungus,
ordinary living cells,
thick specimens,
fungus, stained
specimens, insect
eggs, fat globules,
crystals, etc.
Fungus, protozoa
flagellum, fibrin fibril,
fine granule, section
with a carefully
selected mounting
agent, ultrathin section,
etc.
Fungus, protozoa
flagellum, fibrin fibril,
fine granule, cytometry,
etc.
Spores of fungus,
ordinary living cells,
thick specimens,
fungus, stained
specimens, insect
eggs, fat globules,
crystals, etc.
Fungus, protozoa
flagellum, fibrin fibril,
fine granule, section
with a carefully
selected mounting
agent, ultrathin section,
etc.
Condenser lens to
use for
LWD
N i k o n
M A D E I N C H I N A
Ph code
Ph1