Agilent Technologies InfinityLab LC Series User Manual page 101

4 Preparing the Sampler/Collector
Preparative Sampler Method Parameter Settings
Default settings (preparative and analytical operation mode): 180 µL with 25 %
methanol.
If precipitation occurs in the sampling flow path, increase the organic
composition of the post-sample plug for preparative applications or switch to
plug setting 3. Be aware that the increase of organic composition decreases the
amount of sample that can be injected on the column. The impact is more severe
for analytical injections.
1290 Infinity II Preparative Open-Bed Sampler/Collector User Manual 101