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3
Column Modules
Before trimming the column for the first time make sure the unions are pointed away from
C AU T I O N
the column module as noted in the paragraph above. The union must also be slid to the
position furthest away from the module. This union position will result in the maximum
length of column available for future column trims.
1 Slide the knurled nut and ferrule over the capillary column.
Wear safety glasses to protect your eyes from flying particles while handling, cutting, or
WAR N IN G
installing glass or fused silica capillary columns. Use care in handling these columns to
prevent puncture wounds.
1 Trim the capillary column to a length approximately equal to, but not past the end of the
2 Slide the union bracket onto the post so that the column stops in the end of the union.
3 Slide the ferrule and knurled nut up to the ULM union and tighten the nut using your fingers
To undo this connection, simply remove the knurled nut and gently pull back on the ferrule.
Because the rear part of the re-usable ferrule poorly fits the ULM union, it is easy to pull back
with a small pair of tweezers to dislodge the ferrule if it appears to be stuck in the union. If the
ferrule has not been overly tightened, it should slide off of the column leaving minimal debris
on the column. Typically, these ferrules can be re-used many times.
Alternately, for the In and Out column segments, undo the connection by loosening the nut and
simply pulling the column out. Leave the ferrule in place. To reconnect, insert the column, then
tighten the nut. If changing In or Out column segment diameters, remove the nut from the old
segment and leave the ferrule in place on the column (for future re-use). Install the new
segment using a new ferrule as described above.
60
support post as shown.
Unions with a small inner bore do not allow 0.4 mm and 0.5 mm outer diameter tubing to
pass through the union. These columns must stop in the end of the union. At this position,
the bracket should be firmly clamped onto the post using a 6.4 mm (1/4-inch) open end
wrench.
until the nut is snug (note that this is LESS than finger tight). Because the seal at the ferrule
occurs near the tip of the ferrule, over tightening will crush the tip of the ferrule and the
capillary column. It is recommended that the knurled nut be tightened until it feels snug,
and then tightened an additional 10 degrees.
User Guide
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