Tirf Attachment - Nikon TIRF Instructions Manual

TIRF attachment

3
The main optical unit of the TIRF system incorporates a collimator lens, condenser lens, ND filters,
and a field diaphragm. Excitation light from a laser unit is guided into this unit through an optical-fiber
cable. Excitation light emitted by this unit forms an image on the pupil surface of the objective.
To protect your eyes and skin from exposure to the laser, do not detach the TIRF attachment
from the microscope.
(1) Optical-fiber
cable
(2) Laser position
adjustment knobs
I Name and Function of Each Part
3 TIRF attachment
(3) Field diaphragm
centering knob
(4) ND filter
(5) Indices
PlanApo 60x/1.45
PlanApo100x/1.45
PlanApo60x/1.4
(5) Condenser lens
locking screw
(5) Focal point
adjustment knob
TIRF attachment
- 11 -
(3) Field diaphragm
open/close knob
I