Ise (Electrolyte Analyzer) - Siemens ADVIA 2400 Operator's Manual

ISE (electrolyte analyzer)

The ISE measures the amount of sodium (Na), potassium (K), and chloride (Cl) in serum
or urine samples through voltage measurement by ion-selective electrodes.
The sample-dilution probe (DPP) aspirates the sample for electrolyte analysis. The
electrolyte analysis uses buffer as reagents.
In a two-stage process, the buffer voltage is measured, then the sample voltage is
measured. The difference between these voltages, the reference voltage, and the
temperatures of the liquids determine the concentration of Na, Cl, and K in the sample.
1)
Reference Electrode
2)
Na Electrode
3)
K Electrode
4)
Cl Electrode
5)
Mixer
Figure 1-26. ISE components
To ensure data accuracy, undiluted sample is aspirated from the sample tray and
electrolyte analysis is always performed before photometric sampling.
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6)
Dilution Probe (DPP)
7)
Sample
8)
BPEV
9)
ISE Degassing Unit
10)
Buffer Solution
11)
Buffer Pump (BP)
12)
Temperature Stabilizer
13)
DPEV
14)
DP
15)
Waste Block
ADVIA 2400 Operator's Guide