Agilent Technologies InfinityLab LC Series User Manual page 99

Table 9
Column
Mobile phase
Gradient
Flow rate
Column temperature
Injection volume
FLD settings
This shows the
isofluorescence plot
of emission spectra
for 15 PNAs
(5 µg/ml) with a
fixed excitation
wavelength
(260 nm).
Figure 32
Agilent InfinityLab LC Series 1260 Infinity II FLD User Manual
Conditions for Optimization of PNA analysis according to figures below
Optimization of the time-program for the emission wavelength
Optimizing the Detector
Vydac, 2.1 x 200 mm, PNA, 5 µm
A = water; B = acetonitrile (50 : 50)
3 minutes, 60%
14 minutes, 90%
22 minutes, 100%
0.4 ml/min
18 °C
5 µl
PMT 12,
response time 4 s,
step size 5 nm
Method Development
5
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