Agilent Technologies 1260 Infinity User Manual
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Agilent Technologies 1260 Infinity User Manual

Agilent Technologies 1260 Infinity User Manual

Hplc-chip cube interface
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  • Page 1 Agilent 1260 Infinity HPLC-Chip Cube Interface (G4240A ) User Manual...
  • Page 2 Notices Warranty © Agilent Technologies, Inc. 2010, 2012 defined in FAR 52.227-19(c)(1-2) (June 1987). U.S. Government users will receive No part of this manual may be reproduced in The material contained in this docu- no greater than Limited Rights as defined in any form or by any means (including elec- ment is provided “as is,”...
  • Page 3 In This Guide… This manual gives an introduction to HPLC-Chip technology and explains how to install, maintain and troubleshoot you HPLC-Chip Cube. Introduction to HPLC-Chip Technology Introduction to the Chip Cube Site Preparation Installation Operation Maintenance Procedures Consumables and Maintenance Parts Appendix A Appendix A Safety Information HPLC-Chip Cube Interface User’s Guide...
  • Page 4 HPLC-Chip Cube Interface User’s Guide...

  • Page 5: Table Of Contents

    Contents Content Introduction to HPLC-Chip Technology Overview Chip Types The MS Calibration and Diagnosis Chip, Part No. G4240-61001 The Protein ID Chip, Part No. G4240-62001 Modes of Operation More Chips Introduction to the Chip Cube Intended Use Introduction Power Switch Safety Features Main Assemblies Principle of Operation...
  • Page 6 Contents Accessory Kit System Setup Considerations Single Stack Setup Twin Stacks Preparation of Existing Systems Chip Cube Hardware Installation Operation Chip Loading and Unloading Configuration Configure Loading Pump and Analytical Pump Injection Flush Volume Injector Flush Volume and the use of Injector Programs Operational Parameters Setting up Chip Cube Parameters Chip (Valve Positions / Tip)
  • Page 7 Contents Consumables and Maintenance Parts HPLC-Chips Capillaries Fittings Infusion Spare Parts Valve Rotor Cleaning Tools and Replacement Parts Appendix A Safety Information Safety Information Power Cords Operation Safety Symbols Chemical and Biological Safety Lithium Batteries Information The Waste Electrical and Electronic Equipment (WEEE) Directive (2002/96/EC) Legal Notice HPLC-Chip Cube Interface User’s Guide...
  • Page 8 Contents HPLC-Chip Cube Interface User’s Guide...

  • Page 9: Introduction To Hplc-Chip Technology

    Agilent 1260 Infinity HPLC-Chip Cube MS Interface (G4240A) Chip Cube User’s Guide Introduction to HPLC-Chip Technology Overview Chip Types The MS Calibration and Diagnosis Chip, Part No. G4240-61001 The Protein ID Chip, Part No. G4240-62001 This chapter will provide you with a basic understanding of Agilent’s HPLC-Chip technology and an overview of the currently available chips.

  • Page 10: Overview

    Introduction to HPLC-Chip Technology Overview Overview Traditional nanospray mass spectrometry has proven it's potential as a cost-effective, sensitive and reproducible technique for the identification of peptides at femtomol to atomol levels. However, connecting nano capillaries, columns and valves frequently is a tedious procedure and requires user skills and routine.
  • Page 11 Introduction to HPLC-Chip Technology Overview UV Laser UV Laser CAD Drawing CAD Drawing 1. Ablate 1. Ablate Biocompatible, Biocompatible, 4. Trim 4. Trim polyimide film polyimide film X-Y Stage X-Y Stage 2. Clean 2. Clean HV-ESI contact HV-ESI contact 5. Metallize 5.
  • Page 12 Introduction to HPLC-Chip Technology Overview Stator Rotor Figure 2 Schematic of Chip Cube Valve (Side View) Once the valve is closed, both rotors can rotate in either direction. The use of extremely smooth, hard ceramic rotors guarantees chemical inertness and pressure tightness with virtually no wear on the rotors and very little wear of the chip.

  • Page 13: Chip Types

    The second function of this chip is to calibrate the Chip Cube valve (Agilent 1260 Infinity HPLC-Chip Cube - Service Manual, Valve Calibration). Make sure that port 2 is connected to the nano pump and port 6 to waste. Port 5 should be open or connected to the µ-Well-plate Sampler.

  • Page 14: The Protein Id Chip, Part No. G4240-62001

    Introduction to HPLC-Chip Technology Chip Types The Protein ID Chip, Part No. G4240-62001 This HPLC-Chip is the standard chip for one-dimensional separations. It is comprised of a 40 nL enrichment column and a 75 µm x 43mm separation column which is packed with Zorbax C18-SB 5 µm material. The functionality of this chip is equivalent to conventional nanospray LC/MS.
  • Page 15 Introduction to HPLC-Chip Technology Chip Types When sample loading is finished, the valve switches to the Analyze position. The front end of the enrichment column is now connected to the nano pump via port 2. The outlet end of the enrichment column now connects to the analytical column.
  • Page 16 Introduction to HPLC-Chip Technology Chip Types Backflush mode from µ-WPS to waste enrichment column analytical column from nano pump Figure 6 Loading the sample in backflush mode Port 5 of the HPLC-Chip is connected to the µ-well-plate sampler. When the valve is in the loading position, the sample is transferred at a flow of 4 µL/min onto the enrichment column.
  • Page 17 Introduction to HPLC-Chip Technology Chip Types from µ-WPS to waste enrichment column from nano pump analytical column Figure 7 Analysis in backflush mode As the nanoflow gradient starts, the retained peptides are eluted onto the analytical column where they are separated by reversed phase chromatography.

  • Page 18: More Chips

    Introduction to HPLC-Chip Technology Chip Types More Chips Chips with different packing materials and layouts will become available in future. HPLC-Chip Cube Interface User’s Guide...

  • Page 19: Introduction To The Chip Cube

    Agilent 1260 Infinity HPLC-Chip Cube MS Interface (G4240A) Chip Cube User’s Guide Introduction to the Chip Cube Intended Use Introduction Main Assemblies Principle of Operation Agilent Technologies...

  • Page 20: Intended Use

    Introduction to the Chip Cube Intended Use Intended Use The G4240A Chip Cube is designed and tested as “Controlled Office electrical equipment for laboratory use”. The operator of this instrument is advised that if the equipment is used in a manner C A U T I O N not specified in this manual, the protection provided by the equipment may be impaired.

  • Page 21: Introduction

    • the Chip Cube Interface Assembly (also referred to as Spray Chamber). The HPLC-Chip Cube MS Interface The Chip Cube belongs to the Agilent 1260 Infinity HPLC family. It interfaces the HPLC-Chip to the pump and the µ-well-plate sampler of the nano-LC system.

  • Page 22: Power Switch

    Introduction to the Chip Cube Introduction • Extract the sprayer tip from the chip holder. • Position the sprayer tip reproducible in front of the MS inlet. • Provide two independent, concentrically high pressure nano flow valves. The Chip Cube Interface Assembly The Chip Cube Interface Assembly is effectively a spray chamber for use with the Chip Cube.

  • Page 23: Safety Features

    Introduction to the Chip Cube Introduction To disable the CCD camera and illumination system, disconnect the external power supply from line power. For power ratings and line voltage range see “Power Considerations” page 32. Safety Features Different safety features protect the user from hazards when using the Agilent G4240A HPLC-Chip MS Interface.

  • Page 24: Main Assemblies

    Introduction to the Chip Cube Main Assemblies Main Assemblies Figure 9 Chip Cube Front door / chip loading/unloading button Power switch Status indicator Front door Fan grid Chip loading slot HPLC-Chip Cube Interface User’s Guide...
  • Page 25 Introduction to the Chip Cube Main Assemblies Figure 10 Chip Cube main assemblies Attachment bolts Front door Strain relief of capillary guide Stages assembly Stator assembly with capillary cover Manual clamp release knob Chip feeder assembly Front door / chip loading/unloading button HPLC-Chip Cube Interface User’s Guide...

  • Page 26: Principle Of Operation

    Introduction to the Chip Cube Principle of Operation Principle of Operation The HPLC-Chip/MS Cube is a fully automated device for handling HPLC-Chips. One HPLC-Chip can be used at a time. Chip Loading Once the user has placed an HPLC-Chip into the loading slot of the Chip Cube, all subsequent chip loading actions do not require manual interaction.
  • Page 27 Pump Configuration When used with Agilent 1260 Infinity pumps, ChemStation can configure a loading pump and an analytical pump for the Chip Cube. Doing so is highly recommended as it ensures that the pumps switch OFF when the chip position is not in the Operate position.
  • Page 28 Introduction to the Chip Cube Principle of Operation Typical Spray A typical spray is illustrated in Figure The initial position of the HPLC-Chip tip should be in horizontally centered between end cap and counter electrode. Vertically, the tip should be at the same level as the top counter electrode.

  • Page 29: Flow Connections

    Introduction to the Chip Cube Principle of Operation Flow Connections Capillaries With the Chip Cube, only PEEK-coated fused silica capillaries are used as they offer uniform inner diameter, excellent surface smoothness and have a pressure rating suitable for all nano applications. Fused silica is compatible with all common HPLC solvents.
  • Page 30 Ferrules and Fittings In the Agilent G4240A Chip Cube, only PEEK micro fittings with 4 mm threads are used. However, older Agilent 1260 Infinity Series G2226A nano pumps may require 1/32” double winged fingertight fittings (see: “Fittings” on page 84).

  • Page 31: Site Preparation

    Agilent 1260 Infinity HPLC-Chip Cube MS Interface (G4240A) Chip Cube User’s Guide Site Preparation Power Considerations Bench Space Environment Sound Emission Specifications of the G4240A HPLC-Chip Cube In this chapter you will find information about site requirements for sucessful installation and operation of the G4240A Chip Cube.

  • Page 32: Site Requirements For The G4240A Chip Cube

    WA R N I N G still uses some power, even if the power switch on the front panel is turned off. Never use cables other than the ones supplied by Agilent Technologies to ensure WA R N I N G proper functionality and compliance with safety or EMC regulations.

  • Page 33: Environment

    Site Preparation Site Requirements for the G4240A Chip Cube Approximately 15 cm (6 inches) of clear space behind the instrument is required for air circulation and electric connections. One end of the power cord must always be accessible. For detaching the G4240A Chip Cube from the Chip Cube Interface on the mass spectrometer, allow 10 cm (4 inches) of free space between the Chip Cube and the LC stack.

  • Page 34: Sound Emission

    Site Preparation Site Requirements for the G4240A Chip Cube Sound Emission This statement is provided to comply with the requirements of the German Sound Emission Directive of January 18 1991. This product has a sound pressure emission (at the operator position) <70dB (A).
  • Page 35 Site Preparation Site Requirements for the G4240A Chip Cube Table 4 Performance Specifications Type Specification Comment GLP features Number of injection on chip Chip operation time First date of operation Latest date of operation Communication Controller-area-network (CAN), RS 232C, APG remote standard Safety features Enclosure with interlock system, low voltage in maintenance areas,...
  • Page 36 Site Preparation Site Requirements for the G4240A Chip Cube HPLC-Chip Cube Interface User’s Guide...

  • Page 37: Installation

    Agilent 1260 Infinity HPLC-Chip Cube MS Interface (G4240A) Chip Cube User’s Guide Installation Unpacking the Agilent G4240A HPLC-Chip MS Interface System Setup Considerations Preparation of Existing Systems Chip Cube Hardware Installation This chapter describes how to install the HPLC-Chip/MS Cube.

  • Page 38: Unpacking The Agilent G4240A Hplc-Chip Ms Interface

    Check that all parts and materials have been delivered with the HPLC-Chip Cube MS Interface (G4240A). Compare the shipment content with the checklist included in each instrument box. Please report missing or damaged parts to your local Agilent Technologies sales and service office. HPLC-Chip Cube Interface User’s Guide...
  • Page 39 Installation Unpacking the Agilent G4240A HPLC-Chip MS Interface Table 5 Delivery Checklist for the G4240 HPLC-Chip MS Interface Description Quantity Part Number HPLC-Chip/MS Cube G4240-64000 Power cord, local (matching the sockets of your country or region) Chip Cube Interface Assembly G1982-60050 Monitor B/W, 9 inch G1680-63500...

  • Page 40: Accessory Kit

    Installation Unpacking the Agilent G4240A HPLC-Chip MS Interface Accessory Kit Each G4240A Chip Cube comes with an Accessory Kit which contains the tools and consumables needed to install the instrument and make the first runs. Table 6 Chip Cube Accessory Kit G4240-68705 Item Description Part Number...
  • Page 41 Installation Unpacking the Agilent G4240A HPLC-Chip MS Interface Table 6 Chip Cube Accessory Kit G4240-68705 (continued) Item Description Part Number Abrasive micro mesh 8660-0852 for cleaning of valve rotors Rotor mounting tool G4240-68708 HPLC-Chip Cube Interface User’s Guide...

  • Page 42: System Setup Considerations

    Depending on the available bench space the HPLC can be installed in one or two stacks. In order to keep the solvent capillaries as short as possible, the following two configurations are recommended by Agilent Technologies. HPLC-Chip Cube Interface User’s Guide...

  • Page 43: Single Stack Setup

    Installation System Setup Considerations Single Stack Setup Solvent Compartment Chip Cube Degasser Nano pump Capillary Pump Ion Trap MS Micro Well Plate Sampler Cooler (optional) Figure 13 Recommended Single Stack Configuration - (Front View) In this configuration, only one degasser is used so only one A solvent and one B solvent N O T E can be used for each pump.
  • Page 44 Installation System Setup Considerations Solvent Compartment Degasser Remote start/stop cable Nano Pump GPIB or LAN Chip Cube to LC ChemStation Capillary Pump Ion Trap µ-Well Plate Sampler Cooler (optional) CAN cable APG Remote cable Figure 14 Recommended Single Stack Configuration - (Rear View) If a G1330B ALS thermostat is part of the system it must always be installed underneath N O T E the Micro Well Plate Sampler.

  • Page 45: Twin Stacks

    Installation System Setup Considerations Twin Stacks Solvent Cabinet Degasser Autosampler Solvent Cabinet Degasser Capillary Pump µ-Well Plate Sampler Cooler (optional) Chip Cube Figure 15 Recommended Twin Stack Configuration - (Front View) Setting up the HPLC in two stacks requires 36 cm (14.5 inches) of additional bench space. N O T E For bench space and load specification see “Bench Space”...
  • Page 46 Installation System Setup Considerations Solvent Cabinet APG Remote cable Degasser Chip Cube Nano Pump Solvent Cabinet Degasser Capillary Pump Ion Trap µ-Well Plate Sampler Cooler (optional) APG Remote cable CAN connections Figure 16 Recommended Twin Stack Configuration - Rear View) •...

  • Page 47: Preparation Of Existing Systems

    1 Optimize the Agilent 1260 Infinity stack configuration as described in “System Setup Considerations” on page 42. Allow 36 cm (14”) space between Agilent 1260 Infinity stack and mass spectrometer for the N O T E Chip Cube. 2 Update the firmware of all Agilent 1260 Infinity modules to revision A.06.01 or higher.
  • Page 48 Installation Preparation of Existing Systems 3 Upgrade Chemstation to rev. B.01.03 or later as described in the Chemstation Installation Manual. 4 Install MS control software as described in the HPLC Chip MS interface adapter guide. 5 Replace the MS ion source with the Chip Cube Interface Assembly and install the MS side cover and service door supplied with the Chip Cube Interface kit.

  • Page 49: Chip Cube Hardware Installation

    Chip Cube Interface Assembly (spray chamber). 8 Open the Chip Cube front door and connect the Chip Cube to any free CAN port of the Agilent 1260 Infinity stack using the supplied 1 m CAN cable (see Figure 14 on page 44).
  • Page 50 Installation Chip Cube Hardware Installation 14 Adjust the position of the chip tip to the following position. This alignment is typically only required once. HPLC-Chip tip end cap counter electrode HV-capillary nanospray Figure 17 Optimal chip tip position Vertical adjustment The ideal position chip tip is slightly below the face of the MS end cap (see Figure 17).
  • Page 51 Installation Chip Cube Hardware Installation Horizontal Adjustment Turn the thumb wheel on the left bottom side of the Chip Cube. Move the HPLC-Chip tip further towards the counter electrode until the tip is equidistant to both end cap and counter electrode. hand wheel Figure 19 Position of hand wheel...
  • Page 52 Installation Chip Cube Hardware Installation HPLC-Chip Cube Interface User’s Guide...

  • Page 53: Operation

    Agilent 1260 Infinity HPLC-Chip Cube MS Interface (G4240A) Chip Cube User’s Guide Operation Chip Loading and Unloading Configuration Operational Parameters Setting up Chip Cube Parameters Spray Ignition This chapter explains the operational parameters of the Chip Cube and provides practical hints.

  • Page 54: Chip Loading And Unloading

    Operation Chip Loading and Unloading Chip Loading and Unloading The G4240A Chip Cube features a semiautomatic chip handler. One HPLC-Chip at a time can be placed in the loading slot. Once it has been fully inserted, the chip feeder pulls it in and connects it hydraulically and electrically without manual user interaction.

  • Page 55: Configuration

    Operation Configuration Configuration In Chemstation, left-click the Chip Cube icon or select More Chip Cube from the Instrument menu. Click Configuration to access the configuration dialog box. access to configuration dialog Figure 21 More Chip Cube Dialog Configure Loading Pump and Analytical Pump Before working with the system, it is highly recommended to configure both loading pump and analytical pump for the Chip Cube.

  • Page 56: Injection Flush Volume

    Operation Configuration Figure 22 Pump Configuration Dialog box Usually, the G1376A capillary pump is used as loading pump and the G2226A nano pump acts as the analytical pump. Two nano pumps can be used as well. Select the appropriate pumps from the drop down lists in the configuration dialog box.

  • Page 57: Injector Flush Volume And The Use Of Injector Programs

    Injector Flush Volume and the use of Injector Programs The Chip Cube queries all samplers in the Agilent 1260 Infinity LC stack prior to the run (to be more precise: before the sampler(s) start to move and draw sample) and adds the found injection volumes to the Injector Flush Volume.
  • Page 58 Chip Cube is to use “def. Volume” in injector programs instead of defining specific volumes. Example 3: 1260 Infinity LC system, auto sampler with poor injector program • µWPS injection volume in method: 1 µl •...
  • Page 59 Operation Configuration • Injector program: Draw def. vol from sample Wait 10 s Eject into seat Mainpass IFV: 8 µL ✔ Resulting computed value: 1 µL (default inj. Volume) + 8 µL = 9 µL HPLC-Chip Cube Interface User’s Guide...

  • Page 60: Operational Parameters

    Operation Operational Parameters Operational Parameters The table below gives an overview of typical mobile phases, flow rates and gradients for use with the different HPLC-Chips. The maximum operation pressure of the HPLC-Chips is 150 bar. This value is stored in an RFID tag inside the chip holder handle. If the pressure limit of the Chemstation method is set to a higher value as the maximum operating pressure of the HPLC-Chip, Chemstation opens a popup box and notifies the customer.

  • Page 61: Setting Up Chip Cube Parameters

    Operation Setting up Chip Cube Parameters Setting up Chip Cube Parameters Access this dialog box from the Method & Run Control screen by either left-clicking the Chip Cube icon and selecting Set up Chip Cube or by selecting the same command from the Instrument menu. Figure 23 Chip Cube method parameters Chip (Valve Positions / Tip)

  • Page 62: Time

    Operation Setting up Chip Cube Parameters Protein ID Chip G4240-62001 With this chip the two positions, Enrichment and Analysis, are available. The chip valve must be in Enrichment position at the begin of the run in order to load the sample onto the enrichment column. The outer valve is not used with this chip.

  • Page 63: Time Table

    Operation Setting up Chip Cube Parameters Time Table As an alternative to defining of an Injector Flush Volume (see “Injection Flush Volume” on page 56) a time table can be set up to control the Chip Cube valves. This time table provides full control over the valves, but does not correct automatically for changes in loading pump flow rate and injection volume.

  • Page 64: Chip Details Screen

    Operation Setting up Chip Cube Parameters Figure 24 Instrument Actuals In addition to the Chip Cube status, the actuals window displays information about the valves used with the installed chip, user comments, the position of the chip and the total operation time of the chip. This parameter is an important indicator of the remaining chip life time.
  • Page 65 Operation Setting up Chip Cube Parameters Figure 25 Chip Details dialog box These data are retrieved from an RFID tag which is embedded in the handle of the chip holder. Most parameters on the tag are persistent (product number, serial number, revision, max. pressure, column dimensions and packing material data) whereas diagnostic data are updated while the chip is in operation.

  • Page 66: Spray Ignition

    Operation Spray Ignition Spray Ignition Typically, spray ignition does not require any optimization of the chip position. Table 8 Typical instrument parameters for igniting nanospray Parameter Value Remarks • nanopump 0.3 µL/min 98/2 water/ACN (both with 0.1% formic acid) • loading pump 4 µL/ min 98/2 water/ACN (both with 0.1% formic acid) •...
  • Page 67 Operation Spray Ignition HPLC-Chip tip end cap counter electrode HV-capillary nanospray Figure 26 Good nanospray under highly aqueous conditions Depending on the solvent composition the observed Taylor cone will vary in appearance; at high aqueous composition the plume will appear as a straight jet which will change to a fan-type plume as the organic solvent increases.
  • Page 68 Operation Spray Ignition Do not operate the HPLC-Chip at capillary voltages greater than 2400 V or capillary C A U T I O N currents greater 100 nA. Doing so will decrease the lifetime of the HPLC-Chip. Vertical and Horizontal Tip Positioning The tip positioning is set during installation and usually requires no adjustment for optimal performance.

  • Page 69: Conditions To Avoid

    Operation Spray Ignition Conditions to avoid HPLC-Chip tip end cap counter electrode HV-capillary nano spray Figure 27 Spray angled into the MS entrance The spray must not be angled directly into the MS entrance, this will contaminate the C A U T I O N desolvation assembly of the MS and cause high background.
  • Page 70 Operation Spray Ignition hand wheel Figure 28 Position of the hand wheel If neither action is successful, the tip may be damaged. Replace the HPLC-Chip with a new one. HPLC-Chip Cube Interface User’s Guide...

  • Page 71: Maintenance Procedures

    Agilent 1260 Infinity HPLC-Chip Cube MS Interface (G4240A) Chip Cube User’s Guide Maintenance Procedures Maintenance Overview Replacing Capillaries Cleaning the Valve Rotors (quick procedure) Updating the Chip Cube Firmware This chapter describes the routine maintenance tasks that are recommended to maintain best performance of the HPLC-Chip Cube MS Interface (G4240A).

  • Page 72: Maintenance Overview

    Maintenance Procedures Maintenance Overview Maintenance Overview Table 9 Overview of Maintenance Procedures Procedure Typical Frequency Notes Replacing capillaries When broken, blocked or kinked Be aware of the installation procedure for narrow ID capillaries Valve rotor cleaning (quick procedure) When system pressure test is failing Rotors stay assembled Valve rotor cleaning (thorough When quick rotor cleaning does not...

  • Page 73: Replacing Capillaries

    Maintenance Procedures Replacing Capillaries Replacing Capillaries When required When broken, blocked or kinked Tools required Cube wrench, P/N G4240-83800 Hex key 3 mm, 12 cm long, P/N 8710-2411 Parts required Fused silica/PEEK capillary (see Parts) Preparations • Unload the HPLC-Chip Only perform this procedure if the HPLC-Chip Cube MS Interface (G4240A) is mounted C A U T I O N on the MS or sitting flat on a table as the center of gravity shifts and the instrument will...
  • Page 74 Maintenance Procedures Replacing Capillaries 3 Remove the capillary cover (A) and open the thumb screw 4 Loosen the slotted PEEK fitting using the Cube wrench of the strain relief (B). and pull the capillary out. 5 Push the new capillary through the capillary guide until 6 Connect the capillaries as follows to the Chip Cube valve the stopper is flush with the end of the guide tube.
  • Page 75 Maintenance Procedures Replacing Capillaries 7 Route the capillaries exactly as shown below. 8 Reinstall the capillary cover. Do not overtighten the cover screws!. 9 Flip the stages assembly up and close the front pane. HPLC-Chip Cube Interface User’s Guide...

  • Page 76: Cleaning The Valve Rotors (Quick Procedure)

    Maintenance Procedures Cleaning the Valve Rotors (quick procedure) Cleaning the Valve Rotors (quick procedure) When required When system pressure test fails Tools required Lint-free wipe or lint free piece of cloth Parts required none Preparations • Eject the HPLC-Chip, pumps will turn off automatically (if the pumps are configured for Chip Cube) •...
  • Page 77 Maintenance Procedures Cleaning the Valve Rotors (quick procedure) 1 Press the cover button quickly and open the front cover 2 Open the lock screw of the latch lock and pull it out. once the lock has opened. 3 Unlock the stages assembly and flip it out. 4 Open the red thumb screw and remove the capillary guide tubing from the strain relief.
  • Page 78 Maintenance Procedures Cleaning the Valve Rotors (quick procedure) 5 With capillaries and capillary cover still attached, pull the 6 Wipe the outer and inner rotor thoroughly with a methanol stator assembly out carefully and set it aside. dampened lint free wipe or piece of cloth. 7 Make sure no particles are left in the rotors as they will cause leaks.

  • Page 79: Updating The Chip Cube Firmware

    2 Load the firmware into the Chip Cube as described in the documentation. When using the G4240A Chip Cube in an Agilent 1260 Infinity Series system, all other C A U T I O N Agilent 1260 Infinity Series modules must be upgraded to firmware revision 6.x or above (main and resident).
  • Page 80 Maintenance Procedures Updating the Chip Cube Firmware HPLC-Chip Cube Interface User’s Guide...

  • Page 81: Consumables And Maintenance Parts

    Agilent 1260 Infinity HPLC-Chip Cube MS Interface (G4240A) Chip Cube User’s Guide Consumables and Maintenance Parts HPLC-Chips Capillaries Fittings Infusion Spare Parts This chapter provides information on parts for maintenance. Agilent Technologies...

  • Page 82: Hplc-Chips

    Consumables and Maintenance Parts HPLC-Chips HPLC-Chips Table 10 Standard HPLC-Chips Item Description Part Number MS calibration and diagnostic chip G4240-61001 Protein ID Chip G4240-62001 40 nL trap 75um x 43mm 5um 300SB-C18-ZX The HPLC-Chips described in Table 10 are available at product introduction. Different packaging materials, column dimension and chip layouts will become available later.

  • Page 83: Capillaries

    Consumables and Maintenance Parts Capillaries Capillaries Table 11 PEEK Coated Fused Silica Capillaries For Use With Chip Cube Item Description Part Number Nano pump to Chip Cube, 15 µm x 900 mm, orange or red color G4240-87300 Micro Well Plate Sampler to Chip Cube, 25 µm x 1050 mm, yellow color G4240-87301 Syringe pump to Chip Cube (Infusion), 75 µm x 1000 mm, blue color G4240-87303...

  • Page 84: Fittings

    Consumables and Maintenance Parts Fittings Fittings Table 12 Fittings for Use with Chip Cube Item Description Part Number Chip Cube fitting G4240-43200 M4 PEEK fitting, slotted (1 ea.) M4 PEEK fitting 5065-4410 used in µ-well-plate sampler (pack of 10) Finger tight fitting 5065-4422 double winged nut and 1/32”...

  • Page 85: Infusion Spare Parts

    Consumables and Maintenance Parts Infusion Spare Parts Infusion Spare Parts Table 13 Spare Parts for Infusion and MS Calibration Item Description Part Number Manual syringe, 100 µL, with PTFE tipped plunger and removable needle, 22 5183-4545 gauge, 50 mm length for use with syringe pump Microtight syringe adapter 0100-2273 interfaces PEEK coated fused silica capillaries to syringe needle...

  • Page 86: Valve Rotor Cleaning Tools And Replacement Parts

    Consumables and Maintenance Parts Valve Rotor Cleaning Tools and Replacement Parts Valve Rotor Cleaning Tools and Replacement Parts Table 14 Valve Rotor Cleaning Tools and Replacement Parts Item Description Part Number Abrasive micro mesh 8660-0852 for cleaning of valve rotors Inner valve rotor, 3 grooves G4240-23705 Outer valve rotor, 5 grooves...

  • Page 87: Appendix A Safety Information

    Agilent 1260 Infinity HPLC-Chip Cube MS Interface (G4240A) Chip Cube User’s Guide Appendix A Safety Information Safety Information Power Cords Operation Safety Symbols Chemical and Biological Safety Lithium Batteries Information In this chapter you will find important safety information relevant to the Agilent 1260 Infinity HPLC-Chip Cube Interface G4240.

  • Page 88: Safety Information

    Agilent Technologies assumes no liability for the customer’s failure to comply with these requirements. A warning alerts you to a situation that could cause physical injury or damage to the WA R N I N G equipment.

  • Page 89: Operation

    Appendix A Safety Information Safety Information Never operate your instrument from a power outlet that has no ground connection. Never WA R N I N G Agilent Technologies use a power cord other than the power cord designed for your region.

  • Page 90: Safety Symbols

    Appendix A Safety Information Safety Information Safety Symbols Table 15 Safety Symbols used on Instruments and in Manuals Symbol Description Electrosensitive device. The apparatus is marked with this symbol when the user should refer to the Service Manual in order to prevent harm to the operator and to protect the apparatus against damage.

  • Page 91: Chemical And Biological Safety

    Appendix A Safety Information Safety Information Chemical and Biological Safety When working with solvents please observe appropriate safety procedures (for WA R N I N G example goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet supplied by the solvent vendor, especially when toxic or hazardous solvents are used.

  • Page 92: Lithium Batteries Information

    Appendix A Safety Information Lithium Batteries Information Lithium Batteries Information Danger of explosion if battery is incorrectly replaced. Replace only with the same or WA R N I N G equivalent type recommended by the equipment manufacturer. Lithium batteries may not be disposed-off into the domestic waste. Transportation of discharged Lithium batteries through carriers regulated by IATA/ICAO, ADR, RID, IMDG is not allowed.

  • Page 93: The Waste Electrical And Electronic Equipment (Weee) Directive (2002/96/Ec)

    Appendix A Safety Information The Waste Electrical and Electronic Equipment (WEEE) Directive (2002/96/EC) The Waste Electrical and Electronic Equipment (WEEE) Directive (2002/96/EC) Abstract The Waste Electrical and Electronic Equipment (WEEE) Directive (2002/96/EC), adopted by EU Commission on 13 Feb. 2003, is introducing producer responsibility on all Electric and Electronic appliances from 13 Aug.

  • Page 94: Legal Notice

    Appendix A Safety Information Legal Notice Legal Notice The G4240A Chip Cube is sold for research only. By purchasing this instrument, the purchaser is granted the limited right to use only this instrument. Purchase of this instrument does not include any right, express or implied, to use any other patented product, method or process, or to use any other portion or component of any patented system or systems, software, microfluidic devices or reagents, either alone or...
  • Page 96 Introduction to the Chip Cube • Site Preparation • Installation • Operation • Maintenance Procedures • Consumables and Maintenance Parts Appendix A Safety • Information  Agilent Technologies 2010, 2012 Printed in Germany 05/2012 *G4240-90006* *G4240-90006* G4240-90006 Rev. B Agilent Technologies...

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