Agilent Technologies 1260 Infinity Quick Start Manual page 54

6
Calibration Procedures
Characterizing the delay volumes: Mixing point to UV detector and Column to UV detector
Autosampler
G7129A/B/C
G7167A/B
G4226A
G1377A
G1367E
G1367D
G1313A
G1329A/B
G1367A/B/C
G5667A
1120 Compact LC
1220 VL Compact LC
1220 Compact LC
G5668A
Example of the
Calculation for a custom 1290 Infinity binary system in a set-up using a
calculation of the
thermostat capillary in the TCC module:
Mixing point to
UV detector delay
Part
tubing volume
Pump
Capillary
Autosampler
Capillary
Capillary
Thermostat
Capillary
54
Syringe: Volume, mL
40 µL: 0.04 + injected volume
100 µL: 0.062 + injected volume
900 µL: 0.177 + injected volume
20 µL: 0.08 + injected volume
40 µL: 0.118 + injected volume
100 µL: 0.3 + injected volume
8 µL: 0.048 + injected volume
40 µL: 0.142 + injected volume
40 µL: 0.118 + injected volume
100 µL: 0.3 + injected volume
40 µL: 0.142 + injected volume
100 µL: 0.3 + injected volume
100 µL: 0.3 + injected volume
100 µL: 0.062 + injected volume
Description
1290 Infinity Binary Pump with V100 mixer
Pump to autosampler (0.17 × 200 mm)
1290 Infinity Autosampler with 20 µL loop
Autosampler to TCC (0.12 × 340 mm)
TCC to column (0.12 × 150 mm)
Thermostat (3 µL)
Column to UV detector (0.12 × 280 mm)
Volume, mL
0.160
0.005
0.080 + injected volume
0.004
0.002
0.003
0.003
Purification Solution - Developer's Guide