Microscope Adjustment - Nikon DS-L1 Instructions Manual

When the camera head is mounted on a microscope, adjust the microscope as described below.
(1) Use the Appropriate Filters
When capturing a color image:
Insert the NCB filter into the optical path.
When capturing a black & white image:
Insert the appropriate filter into the optical path, depending on the photographic purpose.
Generally, inserting a green interference filter (GIF) will heighten contrast. Contrast is
enhanced by inserting a filter with a color complementary to the specimen.
Supplement
* The method for inserting and removing a filter varies from microscope to
microscope. Refer to your microscope's instruction manual.
* A third-party color compensation filter (CC filter) can be inserted into the
illumination optical path of the microscope to compensate for changes in
color balance caused by the length of the exposure time.
* When using a phase contrast microscope or an interference microscope (two
luminous fluxes or multiple luminous fluxes), you can enhance contrast
using a green interference filter (GIF) or a monochrome interference filter
(IF).
* Some microscopes may require a heat-wave absorption filter.
Terms
What is an NCB filter?
An NCB (neutral color balance) filter is a color-balancing compensation filter used to adjust
color temperature to daylight values in microscopes that use a halogen bulb as a light
source.
What is a complementary color?
For example, green and magenta, red and cyan, and blue and yellow are all pairs of
complementary colors. When complementary colors overlap, their respective hues cancel
each other out.
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Microscope Adjustment

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